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Protein

Mediator of RNA polymerase II transcription subunit 25

Gene

Med25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 25
Alternative name(s):
Mediator complex subunit 25
Short name:
mMED25
Gene namesi
Name:Med25
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1922863 Med25

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003049531 – 745Mediator of RNA polymerase II transcription subunit 25Add BLAST745

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei723Asymmetric dimethylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ8VCB2
PeptideAtlasiQ8VCB2
PRIDEiQ8VCB2

PTM databases

iPTMnetiQ8VCB2
PhosphoSitePlusiQ8VCB2

Expressioni

Gene expression databases

BgeeiENSMUSG00000002968 Expressed in 270 organ(s), highest expression level in dorsal pancreas
ExpressionAtlasiQ8VCB2 baseline and differential
GenevisibleiQ8VCB2 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CREBBP. Interacts with ESR1, GR and THRB in a ligand-dependent fashion. Binds the Herpes simplex virus activator VP16 (By similarity). Interacts with RARA and RXRA in a ligand-dependent fashion.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217616, 1 interactor
ComplexPortaliCPX-3264 Core mediator complex
IntActiQ8VCB2, 5 interactors
MINTiQ8VCB2
STRINGi10090.ENSMUSP00000003049

Structurei

3D structure databases

ProteinModelPortaliQ8VCB2
SMRiQ8VCB2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 226Interaction with the Mediator complexBy similarityAdd BLAST226
Regioni389 – 543Interaction with VP16By similarityAdd BLAST155
Regioni395 – 545Interaction with CREBBPBy similarityAdd BLAST151
Regioni563 – 652Interaction with RARABy similarityAdd BLAST90
Regioni639 – 705Interaction with RARABy similarityAdd BLAST67

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi645 – 649LXXLL motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi246 – 365Pro-richAdd BLAST120
Compositional biasi546 – 611Gln-richAdd BLAST66

Sequence similaritiesi

Belongs to the Mediator complex subunit 25 family.Curated

Phylogenomic databases

eggNOGiENOG410IHD9 Eukaryota
ENOG410Z0HI LUCA
GeneTreeiENSGT00520000055653
HOGENOMiHOG000113538
HOVERGENiHBG108127
InParanoidiQ8VCB2
KOiK15168
OrthoDBiEOG091G04LS
PhylomeDBiQ8VCB2
TreeFamiTF329598

Family and domain databases

Gene3Di2.40.290.30, 1 hit
InterProiView protein in InterPro
IPR021394 Med25_PTOV
IPR021406 Mediator_Med25_NR-box
IPR021397 Mediator_Med25_SD1
IPR021419 Mediator_Med25_VWA
IPR038196 PTOV_dom_sf
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF11232 Med25, 1 hit
PF11244 Med25_NR-box, 1 hit
PF11235 Med25_SD1, 1 hit
PF11265 Med25_VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q8VCB2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEELRKHYL LPAIEYFNGG
60 70 80 90 100
PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG
110 120 130 140 150
IKFMGGGGES CSLIAEGLST ALQLFDDFKK MREQIGQTHR VCLLICNSPP
160 170 180 190 200
YLLPAVESTT YSGCTTESLV QKIGERGIHF SIVSPRKLPA LRLLFEKAAP
210 220 230 240 250
PALLEPLQQP ADVSQDPRHM VLVRGLVLPV GGSSTSGSLQ TKQAVPLPPA
260 270 280 290 300
PASAATLSAA PPQALPPVPP QYQVPGNLSA AQVAAQNAVE AAKSQKAGLG
310 320 330 340 350
PRFSPINPLQ QAAPGVGPPF SQAPAPPLAP VPPGAPKPPP ASQPSLVSTV
360 370 380 390 400
APGPVLAAPA QPGAPSLAGT VTPGGVNGPS AAQLGGPALG GQQSVSNKLL
410 420 430 440 450
AWSGVLEWQE KPKPASVDAN TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM
460 470 480 490 500
QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL ESLKGLYRIM GNGFAGCVHF
510 520 530 540 550
PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV ITNHKQVQQQ
560 570 580 590 600
KLEQQRGMGA QQAPPVLGPI LEEQARPPQN LLQLRAPQPQ PQGAVGASAA
610 620 630 640 650
TGQPQPQGAT QAPTGAPQGP PGAAPGPPPS GPILRPQNPG ANPQLRSLLL
660 670 680 690 700
NPAPPQTGVP PPQASLHHLQ PPGAPTLLPP HQSMGQPQLG PQLLHPPPAQ
710 720 730 740
SWPTQLPQRA PLPGQMLLSG GPRGPVPQPG LQPSVMEDDI LMDLI
Length:745
Mass (Da):78,113
Last modified:March 1, 2002 - v1
Checksum:iA8C63CF063BECEEA
GO
Isoform 2 (identifier: Q8VCB2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.
     558-581: Missing.

Show »
Length:618
Mass (Da):64,506
Checksum:iFF8E7568526AC470
GO
Isoform 3 (identifier: Q8VCB2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-102: K → KPVLGAEPYP...YEDSTTFGQM

Note: No experimental confirmation available.
Show »
Length:822
Mass (Da):86,585
Checksum:i76EE6D0B4A35AF61
GO
Isoform 4 (identifier: Q8VCB2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-745: Missing.

Show »
Length:102
Mass (Da):10,940
Checksum:i5AF83A18D99C2CEC
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A140LHG7A0A140LHG7_MOUSE
Mediator of RNA polymerase II trans...
Med25
801Annotation score:
A0A140LIH0A0A140LIH0_MOUSE
Mediator of RNA polymerase II trans...
Med25
599Annotation score:
A0A140LHQ0A0A140LHQ0_MOUSE
Mediator of RNA polymerase II trans...
Med25
742Annotation score:
A0A140LIL1A0A140LIL1_MOUSE
Mediator of RNA polymerase II trans...
Med25
199Annotation score:
A0A140LI51A0A140LI51_MOUSE
Mediator of RNA polymerase II trans...
Med25
242Annotation score:
A0A140LJ84A0A140LJ84_MOUSE
Mediator of RNA polymerase II trans...
Med25
69Annotation score:
A0A140LHM8A0A140LHM8_MOUSE
Mediator of RNA polymerase II trans...
Med25
103Annotation score:
A0A140LHI1A0A140LHI1_MOUSE
Mediator of RNA polymerase II trans...
Med25
164Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0281471 – 103Missing in isoform 2. 1 PublicationAdd BLAST103
Alternative sequenceiVSP_028148102K → KPVLGAEPYPDPARMSNSLS LLPANGIPRKLSSGIVKCRC LLRTSGRNPHLEFWEAPRPS LLHIRDGAYEDSTTFGQM in isoform 3. 1 Publication1
Alternative sequenceiVSP_028149103 – 745Missing in isoform 4. 1 PublicationAdd BLAST643
Alternative sequenceiVSP_028150558 – 581Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK148368 mRNA Translation: BAE28509.1
AK149867 mRNA Translation: BAE29135.1
BC021333 mRNA Translation: AAH21333.1
BC031138 mRNA Translation: AAH31138.1
BC071206 mRNA Translation: AAH71206.1
CCDSiCCDS21220.1 [Q8VCB2-1]
CCDS85291.1 [Q8VCB2-2]
RefSeqiNP_001318135.1, NM_001331206.1
NP_001318137.1, NM_001331208.1 [Q8VCB2-2]
NP_083641.1, NM_029365.3 [Q8VCB2-1]
UniGeneiMm.157778

Genome annotation databases

EnsembliENSMUST00000003049; ENSMUSP00000003049; ENSMUSG00000002968 [Q8VCB2-1]
ENSMUST00000207278; ENSMUSP00000146595; ENSMUSG00000002968 [Q8VCB2-2]
GeneIDi75613
KEGGimmu:75613
UCSCiuc009grm.1 mouse [Q8VCB2-2]
uc009grn.1 mouse [Q8VCB2-1]
uc009grs.1 mouse [Q8VCB2-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK148368 mRNA Translation: BAE28509.1
AK149867 mRNA Translation: BAE29135.1
BC021333 mRNA Translation: AAH21333.1
BC031138 mRNA Translation: AAH31138.1
BC071206 mRNA Translation: AAH71206.1
CCDSiCCDS21220.1 [Q8VCB2-1]
CCDS85291.1 [Q8VCB2-2]
RefSeqiNP_001318135.1, NM_001331206.1
NP_001318137.1, NM_001331208.1 [Q8VCB2-2]
NP_083641.1, NM_029365.3 [Q8VCB2-1]
UniGeneiMm.157778

3D structure databases

ProteinModelPortaliQ8VCB2
SMRiQ8VCB2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217616, 1 interactor
ComplexPortaliCPX-3264 Core mediator complex
IntActiQ8VCB2, 5 interactors
MINTiQ8VCB2
STRINGi10090.ENSMUSP00000003049

PTM databases

iPTMnetiQ8VCB2
PhosphoSitePlusiQ8VCB2

Proteomic databases

PaxDbiQ8VCB2
PeptideAtlasiQ8VCB2
PRIDEiQ8VCB2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003049; ENSMUSP00000003049; ENSMUSG00000002968 [Q8VCB2-1]
ENSMUST00000207278; ENSMUSP00000146595; ENSMUSG00000002968 [Q8VCB2-2]
GeneIDi75613
KEGGimmu:75613
UCSCiuc009grm.1 mouse [Q8VCB2-2]
uc009grn.1 mouse [Q8VCB2-1]
uc009grs.1 mouse [Q8VCB2-3]

Organism-specific databases

CTDi81857
MGIiMGI:1922863 Med25

Phylogenomic databases

eggNOGiENOG410IHD9 Eukaryota
ENOG410Z0HI LUCA
GeneTreeiENSGT00520000055653
HOGENOMiHOG000113538
HOVERGENiHBG108127
InParanoidiQ8VCB2
KOiK15168
OrthoDBiEOG091G04LS
PhylomeDBiQ8VCB2
TreeFamiTF329598

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Miscellaneous databases

ChiTaRSiMed25 mouse
PROiPR:Q8VCB2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002968 Expressed in 270 organ(s), highest expression level in dorsal pancreas
ExpressionAtlasiQ8VCB2 baseline and differential
GenevisibleiQ8VCB2 MM

Family and domain databases

Gene3Di2.40.290.30, 1 hit
InterProiView protein in InterPro
IPR021394 Med25_PTOV
IPR021406 Mediator_Med25_NR-box
IPR021397 Mediator_Med25_SD1
IPR021419 Mediator_Med25_VWA
IPR038196 PTOV_dom_sf
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF11232 Med25, 1 hit
PF11244 Med25_NR-box, 1 hit
PF11235 Med25_SD1, 1 hit
PF11265 Med25_VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED25_MOUSE
AccessioniPrimary (citable) accession number: Q8VCB2
Secondary accession number(s): Q3UDX6, Q3UFQ1, Q6IR27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 1, 2002
Last modified: September 12, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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