UniProtKB - Q8VC90 (ZDH12_MOUSE)
Protein
Palmitoyltransferase ZDHHC12
Gene
Zdhhc12
Organism
Mus musculus (Mouse)
Status
Functioni
Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates (PubMed:25025157). Has a palmitoyltransferase activity toward gephyrin/GPHN, regulating its clustering at synapses and its function in gamma-aminobutyric acid receptor clustering (PubMed:25025157). Thereby, indirectly regulates GABAergic synaptic transmission (PubMed:25025157).1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 127 | S-palmitoyl cysteine intermediate1 Publication | 1 |
GO - Molecular functioni
- palmitoyltransferase activity Source: MGI
- protein-cysteine S-palmitoyltransferase activity Source: UniProtKB
GO - Biological processi
- gephyrin clustering involved in postsynaptic density assembly Source: UniProtKB
- peptidyl-L-cysteine S-palmitoylation Source: UniProtKB
- positive regulation of synaptic transmission, GABAergic Source: UniProtKB
- protein palmitoylation Source: MGI
- protein targeting to membrane Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Names & Taxonomyi
Protein namesi | Recommended name: Palmitoyltransferase ZDHHC121 Publication (EC:2.3.1.2251 Publication)Alternative name(s): DHHC domain-containing cysteine-rich protein 12By similarity Short name: DHHC-12By similarity Zinc finger DHHC domain-containing protein 12Imported |
Gene namesi | Name:Zdhhc12Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913470, Zdhhc12 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicCurated | 9 | |
Transmembranei | 10 – 30 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 31 – 43 | LumenalCuratedAdd BLAST | 13 | |
Transmembranei | 44 – 64 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 65 – 140 | CytoplasmicCuratedAdd BLAST | 76 | |
Transmembranei | 141 – 161 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 162 – 178 | LumenalCuratedAdd BLAST | 17 | |
Transmembranei | 179 – 199 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 200 – 267 | CytoplasmicCuratedAdd BLAST | 68 |
Keywords - Cellular componenti
Endoplasmic reticulum, Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 127 | C → S: Loss of protein-cysteine S-palmitoyltransferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212885 | 1 – 267 | Palmitoyltransferase ZDHHC12Add BLAST | 267 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
EPDi | Q8VC90 |
PeptideAtlasi | Q8VC90 |
PRIDEi | Q8VC90 |
PTM databases
PhosphoSitePlusi | Q8VC90 |
SwissPalmi | Q8VC90 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000015335, Expressed in adult mammalian kidney and 235 other tissues |
Genevisiblei | Q8VC90, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000080521 |
Miscellaneous databases
RNActi | Q8VC90, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 97 – 147 | DHHCPROSITE-ProRule annotationAdd BLAST | 51 |
Domaini
The DHHC domain is required for palmitoyltransferase activity.By similarity
Sequence similaritiesi
Belongs to the DHHC palmitoyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000156902 |
HOGENOMi | CLU_031257_2_0_1 |
InParanoidi | Q8VC90 |
OMAi | RRCRYCM |
OrthoDBi | 1440636at2759 |
PhylomeDBi | Q8VC90 |
TreeFami | TF329809 |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q8VC90-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALWPPLNSG MLVRTGHTVL TWGITLVLFL HDTELRQWEE QGELLLPLTF
60 70 80 90 100
LLLVLSSLLL YLAVSLMDPG YVTTQPQPQG EPKEEQAAMV PQAVPLRRCR
110 120 130 140 150
HCLVLQPLRA RHCRDCRRCV RRYDHHCPWM ENCVGERNHP LFVAYLALQL
160 170 180 190 200
VVLLWGLCLA WSGLQFFQPW GLWLRSTGLL FTTFLLLSFF ALVVALLLAS
210 220 230 240 250
HLYLVARNTT TWEFISSHRI AYLRQRTSNP FDRGPTRNLA HFFCGWPSGP
260
WETLSAEEEE EGSSQVV
Sequence cautioni
The sequence AK013074 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 20 | L → P in AK013074 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 179 | L → F in BAC39965 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006946 | 34 | E → GEPDTAPPRAHPITQ in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK087676 mRNA Translation: BAC39965.1 AK013074 mRNA No translation available. BC021432 mRNA Translation: AAH21432.1 |
CCDSi | CCDS15868.1 [Q8VC90-1] CCDS15869.1 [Q8VC90-2] |
RefSeqi | NP_001032851.1, NM_001037762.1 [Q8VC90-1] NP_079704.2, NM_025428.2 [Q8VC90-2] |
Genome annotation databases
Ensembli | ENSMUST00000081838; ENSMUSP00000080521; ENSMUSG00000015335 [Q8VC90-2] ENSMUST00000102865; ENSMUSP00000099929; ENSMUSG00000015335 [Q8VC90-1] |
GeneIDi | 66220 |
KEGGi | mmu:66220 |
UCSCi | uc008jbd.1, mouse [Q8VC90-2] uc008jbe.1, mouse [Q8VC90-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK087676 mRNA Translation: BAC39965.1 AK013074 mRNA No translation available. BC021432 mRNA Translation: AAH21432.1 |
CCDSi | CCDS15868.1 [Q8VC90-1] CCDS15869.1 [Q8VC90-2] |
RefSeqi | NP_001032851.1, NM_001037762.1 [Q8VC90-1] NP_079704.2, NM_025428.2 [Q8VC90-2] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000080521 |
PTM databases
PhosphoSitePlusi | Q8VC90 |
SwissPalmi | Q8VC90 |
Proteomic databases
EPDi | Q8VC90 |
PeptideAtlasi | Q8VC90 |
PRIDEi | Q8VC90 |
Protocols and materials databases
Antibodypediai | 67200, 60 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000081838; ENSMUSP00000080521; ENSMUSG00000015335 [Q8VC90-2] ENSMUST00000102865; ENSMUSP00000099929; ENSMUSG00000015335 [Q8VC90-1] |
GeneIDi | 66220 |
KEGGi | mmu:66220 |
UCSCi | uc008jbd.1, mouse [Q8VC90-2] uc008jbe.1, mouse [Q8VC90-1] |
Organism-specific databases
CTDi | 84885 |
MGIi | MGI:1913470, Zdhhc12 |
Phylogenomic databases
eggNOGi | KOG1311, Eukaryota |
GeneTreei | ENSGT00940000156902 |
HOGENOMi | CLU_031257_2_0_1 |
InParanoidi | Q8VC90 |
OMAi | RRCRYCM |
OrthoDBi | 1440636at2759 |
PhylomeDBi | Q8VC90 |
TreeFami | TF329809 |
Miscellaneous databases
BioGRID-ORCSi | 66220, 0 hits in 17 CRISPR screens |
PROi | PR:Q8VC90 |
RNActi | Q8VC90, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015335, Expressed in adult mammalian kidney and 235 other tissues |
Genevisiblei | Q8VC90, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001594, Palmitoyltrfase_DHHC |
Pfami | View protein in Pfam PF01529, DHHC, 1 hit |
PROSITEi | View protein in PROSITE PS50216, DHHC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZDH12_MOUSE | |
Accessioni | Q8VC90Primary (citable) accession number: Q8VC90 Secondary accession number(s): Q9CZ24, Q9D0T6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | March 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families