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Protein

Afadin- and alpha-actinin-binding protein

Gene

Ssx2ip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (PubMed:12446711). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (PubMed:22027834). Involved in ciliogenesis (By similarity). It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell adhesion, Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Afadin- and alpha-actinin-binding protein
Short name:
ADIP
Alternative name(s):
Afadin DIL domain-interacting protein
Gene namesi
Name:Ssx2ip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2139150 Ssx2ip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000644561 – 615Afadin- and alpha-actinin-binding proteinAdd BLAST615

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei290PhosphoserineBy similarity1
Modified residuei293PhosphoserineBy similarity1
Modified residuei313PhosphoserineCombined sources1
Modified residuei319PhosphoserineCombined sources1
Modified residuei537PhosphoserineBy similarity1
Modified residuei541PhosphoserineBy similarity1
Modified residuei543PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8VC66
PaxDbiQ8VC66
PRIDEiQ8VC66

PTM databases

iPTMnetiQ8VC66
PhosphoSitePlusiQ8VC66

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000036825 Expressed in 288 organ(s), highest expression level in olfactory epithelium
ExpressionAtlasiQ8VC66 baseline and differential
GenevisibleiQ8VC66 MM

Interactioni

Subunit structurei

Interacts with SSX2 and SSX3 (By similarity). Does not interact with SSX1 and SSX4 (By similarity). Interacts with afadin and alpha-actinin (PubMed:12446711). Interacts with VAV2 (PubMed:22027834). Interacts with PCM1 (PubMed:24356449). Interacts with WRAP73 (By similarity).By similarity3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8VC66, 7 interactors
STRINGi10090.ENSMUSP00000101759

Structurei

3D structure databases

ProteinModelPortaliQ8VC66
SMRiQ8VC66
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili126 – 227Sequence analysisAdd BLAST102
Coiled coili266 – 293Sequence analysisAdd BLAST28
Coiled coili375 – 461Sequence analysisAdd BLAST87

Sequence similaritiesi

Belongs to the ADIP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IED3 Eukaryota
ENOG410XWZ9 LUCA
GeneTreeiENSGT00390000007688
HOGENOMiHOG000033818
HOVERGENiHBG050460
InParanoidiQ8VC66
KOiK06085
OrthoDBiEOG091G0HRZ
TreeFamiTF332889

Family and domain databases

InterProiView protein in InterPro
IPR021622 Afadin/alpha-actinin-bd
PfamiView protein in Pfam
PF11559 ADIP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q8VC66-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDWMTVTDP VLCTENKNLS QYTSETKMSP SSLYSQQVLC SSVPLSKNVH
60 70 80 90 100
GVFGVFCTGE NIEQSISYLD QELTTFGFPS LYEESKSKEA KRELNIVAVL
110 120 130 140 150
NCMNELLVLQ RKNLLAQESV ETQNLKLGSD MDHLQSCYAK LKEQLETSRR
160 170 180 190 200
EMIGLQERDR QLQCKNRSLH QLLKNEKDEV QKLQNIIASR ATQYNHDVKR
210 220 230 240 250
KEREYNKLKE RLHQLVMNKK DKNIAMDVLN YVGRADGKRG SWRTDKTEAR
260 270 280 290 300
NEDEMYKILL NDYEYRQKQI LMENAELKKV LQQMKKEMIS LLSPQKKKPR
310 320 330 340 350
ERAEDGTGTV AISDIEDDSG ELSRDSVWGL SCDTVREQLT NSIRKQWRIL
360 370 380 390 400
KSHVEKLDNQ ASKVHSEGLN EEDVISRQDH EQETEKLELE IERCKEMIKA
410 420 430 440 450
QQQLLQQQLA TTCDDDTTSL LRDCYLLEEK ERLKEEWTLF KEQKKNFERE
460 470 480 490 500
RRSFTEAAIR LGLERKAFEE ERASWVKQQF LNMTNFDHQN SENVKLFSAF
510 520 530 540 550
SGSSDPDNLI VHSRPRQKKL HSVANGVPAC TSKLTKSLPA SPSTSDFRQT
560 570 580 590 600
HSCVSEHSSI SVLNITPEES KPSEVAREST DQKWSVQSRP SSREGCYSGC
610
SSAFRSAHGD RDDLP
Length:615
Mass (Da):70,956
Last modified:March 1, 2002 - v1
Checksum:iCC41D7078591919F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4J1A9A0A0R4J1A9_MOUSE
Afadin- and alpha-actinin-binding p...
Ssx2ip mCG_120795
614Annotation score:
D3Z474D3Z474_MOUSE
Afadin- and alpha-actinin-binding p...
Ssx2ip
134Annotation score:
D3Z6T5D3Z6T5_MOUSE
Afadin- and alpha-actinin-binding p...
Ssx2ip
44Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47K → R in AAH31527 (PubMed:15489334).Curated1
Sequence conflicti406Missing in BAC33536 (PubMed:16141072).Curated1
Sequence conflicti406Missing in BAC27363 (PubMed:16141072).Curated1
Sequence conflicti503S → SKPG in BAC33536 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532969 mRNA Translation: AAO15015.1
AK049080 mRNA Translation: BAC33536.1
AK031356 mRNA Translation: BAC27363.1
AK043865 mRNA Translation: BAC31684.1
BC021749 mRNA Translation: AAH21749.1
BC031527 mRNA Translation: AAH31527.1
CCDSiCCDS38665.1
RefSeqiNP_001240697.1, NM_001253768.1
NP_001240698.1, NM_001253769.1
NP_001240699.1, NM_001253770.1
NP_620083.1, NM_138744.3
XP_006502467.1, XM_006502404.3
XP_006502468.1, XM_006502405.3
XP_006502469.1, XM_006502406.3
UniGeneiMm.200783

Genome annotation databases

EnsembliENSMUST00000106153; ENSMUSP00000101759; ENSMUSG00000036825
GeneIDi99167
KEGGimmu:99167
UCSCiuc008rrb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF532969 mRNA Translation: AAO15015.1
AK049080 mRNA Translation: BAC33536.1
AK031356 mRNA Translation: BAC27363.1
AK043865 mRNA Translation: BAC31684.1
BC021749 mRNA Translation: AAH21749.1
BC031527 mRNA Translation: AAH31527.1
CCDSiCCDS38665.1
RefSeqiNP_001240697.1, NM_001253768.1
NP_001240698.1, NM_001253769.1
NP_001240699.1, NM_001253770.1
NP_620083.1, NM_138744.3
XP_006502467.1, XM_006502404.3
XP_006502468.1, XM_006502405.3
XP_006502469.1, XM_006502406.3
UniGeneiMm.200783

3D structure databases

ProteinModelPortaliQ8VC66
SMRiQ8VC66
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VC66, 7 interactors
STRINGi10090.ENSMUSP00000101759

PTM databases

iPTMnetiQ8VC66
PhosphoSitePlusiQ8VC66

Proteomic databases

MaxQBiQ8VC66
PaxDbiQ8VC66
PRIDEiQ8VC66

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106153; ENSMUSP00000101759; ENSMUSG00000036825
GeneIDi99167
KEGGimmu:99167
UCSCiuc008rrb.1 mouse

Organism-specific databases

CTDi117178
MGIiMGI:2139150 Ssx2ip

Phylogenomic databases

eggNOGiENOG410IED3 Eukaryota
ENOG410XWZ9 LUCA
GeneTreeiENSGT00390000007688
HOGENOMiHOG000033818
HOVERGENiHBG050460
InParanoidiQ8VC66
KOiK06085
OrthoDBiEOG091G0HRZ
TreeFamiTF332889

Miscellaneous databases

ChiTaRSiSsx2ip mouse
PROiPR:Q8VC66
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036825 Expressed in 288 organ(s), highest expression level in olfactory epithelium
ExpressionAtlasiQ8VC66 baseline and differential
GenevisibleiQ8VC66 MM

Family and domain databases

InterProiView protein in InterPro
IPR021622 Afadin/alpha-actinin-bd
PfamiView protein in Pfam
PF11559 ADIP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiADIP_MOUSE
AccessioniPrimary (citable) accession number: Q8VC66
Secondary accession number(s): Q8BG59, Q8C7X0, Q8K2F7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2002
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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