Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium/calmodulin-dependent protein kinase kinase 1

Gene

Camkk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by Ca2+/calmodulin. Binding of calmodulin may relieve intrasteric autoinhibition. Partially inhibited upon phosphorylation by PRCAKA/PKA (By similarity). May be regulated through phosphorylation by CAMK1 and CAMK4.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei157ATPPROSITE-ProRule annotation1
Active sitei275Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 142ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: MGI
  • calmodulin-dependent protein kinase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-442717 CREB phosphorylation through the activation of CaMKK

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase kinase 1 (EC:2.7.11.17)
Short name:
CaM-KK 1
Short name:
CaM-kinase kinase 1
Short name:
CaMKK 1
Alternative name(s):
CaM-kinase IV kinase
Calcium/calmodulin-dependent protein kinase kinase alpha
Short name:
CaM-KK alpha
Short name:
CaM-kinase kinase alpha
Short name:
CaMKK alpha
Gene namesi
Name:Camkk1
Synonyms:Camkk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1891766 Camkk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861421 – 505Calcium/calmodulin-dependent protein kinase kinase 1Add BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67PhosphoserineBy similarity1
Modified residuei74PhosphoserineCombined sources1
Modified residuei78Asymmetric dimethylarginineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Modified residuei108PhosphothreonineBy similarity1
Modified residuei458PhosphoserineCombined sources1
Modified residuei475PhosphoserineBy similarity1
Modified residuei492PhosphoserineCombined sources1

Post-translational modificationi

Appears to be autophosphorylated in a Ca2+/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca2+/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4 (By similarity).By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ8VBY2
MaxQBiQ8VBY2
PaxDbiQ8VBY2
PeptideAtlasiQ8VBY2
PRIDEiQ8VBY2

PTM databases

iPTMnetiQ8VBY2
PhosphoSitePlusiQ8VBY2

Expressioni

Tissue specificityi

Widely expressed. Differentially expressed in various brain regions.1 Publication

Gene expression databases

BgeeiENSMUSG00000020785
ExpressionAtlasiQ8VBY2 baseline and differential
GenevisibleiQ8VBY2 MM

Interactioni

Subunit structurei

Interacts with CAMK4 and calmodulin.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8VBY2, 1 interactor
MINTiQ8VBY2
STRINGi10090.ENSMUSP00000090613

Structurei

3D structure databases

ProteinModelPortaliQ8VBY2
SMRiQ8VBY2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini128 – 409Protein kinasePROSITE-ProRule annotationAdd BLAST282

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 189RP domainAdd BLAST23
Regioni435 – 440Autoinhibitory domainBy similarity6
Regioni438 – 463Calmodulin-bindingBy similarityAdd BLAST26

Domaini

The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0585 Eukaryota
ENOG410YHHF LUCA
GeneTreeiENSGT00530000063214
HOGENOMiHOG000007846
HOVERGENiHBG052262
InParanoidiQ8VBY2
KOiK00908
OMAiLHCQKIV
OrthoDBiEOG091G01DB
PhylomeDBiQ8VBY2
TreeFamiTF313013

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q8VBY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGPAVCCQ DPRAELVDRV AAINVAHLEE ADEGPEPARN GVDPPPRARA
60 70 80 90 100
ASVIPGSASR PTPVRPSLSA RKFSLQERPA GSCLGAQVGP YSTGPASHIS
110 120 130 140 150
PRSWRRPTIE SHRVAISDTE DCVQLNQYKL QSEIGKGAYG VVRLAYNESE
160 170 180 190 200
DRHYAMKVLS KKKLLKQYGF PRRPPPRGSQ ATQGGPAKQL LPLERVYQEI
210 220 230 240 250
AILKKLDHVN VVKLIEVLDD PAEDNLYLVF DLLRKGPVME VPCDKPFPEE
260 270 280 290 300
QARLYLRDII LGLEYLHCQK IVHRDIKPSN LLLGDDGHVK IADFGVSNQF
310 320 330 340 350
EGNDAQLSST AGTPAFMAPE AISDSGQSFS GKALDVWATG VTLYCFVYGK
360 370 380 390 400
CPFIDDYILT LHRKIKNEAV VFPEEPEVSE DLKDLILRML DKNPETRIGV
410 420 430 440 450
SDIKLHPWVT KHGEEPLPSE EEHCSVVEVT EEEVKNSVRL IPSWTTVILV
460 470 480 490 500
KSMLRKRSFG NPFEPQARRE ERSMSAPGSL LMKEGCGEGC KSPELPGVQE

DEAAS
Length:505
Mass (Da):55,838
Last modified:March 1, 2002 - v1
Checksum:i179915A25628F9C4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti99 – 100IS → MC in AAF08348 (PubMed:10560916).Curated2
Sequence conflicti198 – 199QE → HD in AAF08348 (PubMed:10560916).Curated2
Sequence conflicti382L → S in AAF08348 (PubMed:10560916).Curated1
Sequence conflicti417L → I in AAF08348 (PubMed:10560916).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117384 mRNA Translation: AAF08348.1
AF461706
, AF461702, AF461703, AF461705, AF461704 Genomic DNA Translation: AAL67849.1
BC017529 mRNA Translation: AAH17529.1
CCDSiCCDS24993.1
RefSeqiNP_061371.2, NM_018883.2
XP_006533823.1, XM_006533760.3
XP_006533824.1, XM_006533761.3
UniGeneiMm.9998

Genome annotation databases

EnsembliENSMUST00000092937; ENSMUSP00000090613; ENSMUSG00000020785
GeneIDi55984
KEGGimmu:55984
UCSCiuc007jzt.1 mouse

Similar proteinsi

Entry informationi

Entry nameiKKCC1_MOUSE
AccessioniPrimary (citable) accession number: Q8VBY2
Secondary accession number(s): Q9R054
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2002
Last modified: July 18, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health