UniProtKB - Q8VBV3 (EXOS2_MOUSE)
Exosome complex component RRP4
Exosc2
Functioni
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC2 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC4 and EXOSC7 (By similarity).
By similarityGO - Molecular functioni
- RNA binding Source: GO_Central
GO - Biological processi
- CUT catabolic process Source: GO_Central
- exonucleolytic catabolism of deadenylated mRNA Source: GO_Central
- exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
- nuclear mRNA surveillance Source: GO_Central
- nuclear polyadenylation-dependent rRNA catabolic process Source: GO_Central
- nuclear polyadenylation-dependent tRNA catabolic process Source: GO_Central
- nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: GO_Central
- polyadenylation-dependent snoRNA 3'-end processing Source: GO_Central
- positive regulation of cell growth Source: MGI
- RNA catabolic process Source: MGI
- RNA processing Source: MGI
- U4 snRNA 3'-end processing Source: GO_Central
Keywordsi
Molecular function | RNA-binding |
Biological process | rRNA processing |
Enzyme and pathway databases
Reactomei | R-MMU-429958, mRNA decay by 3' to 5' exoribonuclease R-MMU-450385, Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA R-MMU-450513, Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA R-MMU-450604, KSRP (KHSRP) binds and destabilizes mRNA R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: Exosome complex component RRP4Alternative name(s): Exosome component 2 Ribosomal RNA-processing protein 4 |
Gene namesi | Name:Exosc2 Synonyms:Rrp4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2385133, Exosc2 |
VEuPathDBi | HostDB:ENSMUSG00000039356 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear exosome (RNase complex) Source: MGI
- nucleolar exosome (RNase complex) Source: ComplexPortal
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- cytoplasmic exosome (RNase complex) Source: GO_Central
- exosome (RNase complex) Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Exosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087130 | 1 – 293 | Exosome complex component RRP4Add BLAST | 293 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 124 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8VBV3 |
MaxQBi | Q8VBV3 |
PaxDbi | Q8VBV3 |
PRIDEi | Q8VBV3 |
ProteomicsDBi | 267673 |
PTM databases
iPTMneti | Q8VBV3 |
PhosphoSitePlusi | Q8VBV3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000039356, Expressed in epiblast (generic) and 248 other tissues |
ExpressionAtlasi | Q8VBV3, baseline and differential |
Genevisiblei | Q8VBV3, MM |
Interactioni
Subunit structurei
Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core (Exo-9) complex which is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric ring of RNase PH domain-containing subunits specifically containing the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and peripheral S1 domain-containing components EXOSC1, EXOSC2 and EXOSC3 located on the top of the ring structure.
Interacts with DIS3.
Interacts with GTPBP1.
Interacts with ZFP36L1 (via N-terminus).
By similarityProtein-protein interaction databases
BioGRIDi | 230671, 3 interactors |
ComplexPortali | CPX-594, Nuclear exosome complex, Dis3-Exosc10 variant CPX-595, Nucleolar exosome complex, Exosc10 variant CPX-596, Cytoplasmic exosome complex, Dis3l variant CPX-598, Exosome complex, Dis3 variant CPX-601, Cytoplasmic exosome complex, Dis3l-Exosc10 variant |
IntActi | Q8VBV3, 1 interactor |
STRINGi | 10090.ENSMUSP00000043519 |
Miscellaneous databases
RNActi | Q8VBV3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 79 – 159 | S1 motifAdd BLAST | 81 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 20 | DisorderedSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3013, Eukaryota |
GeneTreei | ENSGT00940000153596 |
HOGENOMi | CLU_034114_3_1_1 |
InParanoidi | Q8VBV3 |
OMAi | GVNGFIW |
OrthoDBi | 1121868at2759 |
PhylomeDBi | Q8VBV3 |
TreeFami | TF105623 |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit |
InterProi | View protein in InterPro IPR025721, Exosome_cplx_N_dom IPR026699, Exosome_RNA_bind1/RRP40/RRP4 IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR012340, NA-bd_OB-fold |
PANTHERi | PTHR21321, PTHR21321, 1 hit |
Pfami | View protein in Pfam PF14382, ECR1_N, 1 hit PF15985, KH_6, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF54791, SSF54791, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MALEMRLPKA RKPLSESLGR DSKKHLVVPG DTITTDTGFM RGHGTYMGEE
60 70 80 90 100
KLIASVAGSV ERVNKLICVK ALKTRYNGEV GDIVVGRITE VQQKRWKVET
110 120 130 140 150
NSRLDSVLLL SSMNLPGGEL RRRSAEDELA MRGFLQEGDL ISAEVQAVFS
160 170 180 190 200
DGAVSLHTRS LKYGKLGQGV LVQVSPSLVK RQKTHFHDLP CGASVILGNN
210 220 230 240 250
GFIWIYPTPE HKDEDAGGFI ANLEPVALSD REVISRLRNC VVLLVTQRMM
260 270 280 290
LFDTSILYCY EASLAHQIKD ILKPEVMEEI MLETRQRLLD QEG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AV19 | A2AV19_MOUSE | Ribosomal RNA-processing protein 4 | Exosc2 | 247 | Annotation score: | ||
A0A0A6YW57 | A0A0A6YW57_MOUSE | Exosome complex component RRP4 | Exosc2 | 74 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC021485 mRNA Translation: AAH21485.1 BC021807 mRNA Translation: AAH21807.1 |
CCDSi | CCDS15900.1 |
RefSeqi | NP_659135.1, NM_144886.3 |
Genome annotation databases
Ensembli | ENSMUST00000038474; ENSMUSP00000043519; ENSMUSG00000039356 |
GeneIDi | 227715 |
KEGGi | mmu:227715 |
UCSCi | uc008jdy.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC021485 mRNA Translation: AAH21485.1 BC021807 mRNA Translation: AAH21807.1 |
CCDSi | CCDS15900.1 |
RefSeqi | NP_659135.1, NM_144886.3 |
3D structure databases
AlphaFoldDBi | Q8VBV3 |
SMRi | Q8VBV3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 230671, 3 interactors |
ComplexPortali | CPX-594, Nuclear exosome complex, Dis3-Exosc10 variant CPX-595, Nucleolar exosome complex, Exosc10 variant CPX-596, Cytoplasmic exosome complex, Dis3l variant CPX-598, Exosome complex, Dis3 variant CPX-601, Cytoplasmic exosome complex, Dis3l-Exosc10 variant |
IntActi | Q8VBV3, 1 interactor |
STRINGi | 10090.ENSMUSP00000043519 |
PTM databases
iPTMneti | Q8VBV3 |
PhosphoSitePlusi | Q8VBV3 |
Proteomic databases
EPDi | Q8VBV3 |
MaxQBi | Q8VBV3 |
PaxDbi | Q8VBV3 |
PRIDEi | Q8VBV3 |
ProteomicsDBi | 267673 |
Protocols and materials databases
Antibodypediai | 17999, 181 antibodies from 28 providers |
DNASUi | 227715 |
Genome annotation databases
Ensembli | ENSMUST00000038474; ENSMUSP00000043519; ENSMUSG00000039356 |
GeneIDi | 227715 |
KEGGi | mmu:227715 |
UCSCi | uc008jdy.1, mouse |
Organism-specific databases
CTDi | 23404 |
MGIi | MGI:2385133, Exosc2 |
VEuPathDBi | HostDB:ENSMUSG00000039356 |
Phylogenomic databases
eggNOGi | KOG3013, Eukaryota |
GeneTreei | ENSGT00940000153596 |
HOGENOMi | CLU_034114_3_1_1 |
InParanoidi | Q8VBV3 |
OMAi | GVNGFIW |
OrthoDBi | 1121868at2759 |
PhylomeDBi | Q8VBV3 |
TreeFami | TF105623 |
Enzyme and pathway databases
Reactomei | R-MMU-429958, mRNA decay by 3' to 5' exoribonuclease R-MMU-450385, Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA R-MMU-450513, Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA R-MMU-450604, KSRP (KHSRP) binds and destabilizes mRNA R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Miscellaneous databases
BioGRID-ORCSi | 227715, 29 hits in 66 CRISPR screens |
ChiTaRSi | Exosc2, mouse |
PROi | PR:Q8VBV3 |
RNActi | Q8VBV3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039356, Expressed in epiblast (generic) and 248 other tissues |
ExpressionAtlasi | Q8VBV3, baseline and differential |
Genevisiblei | Q8VBV3, MM |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit |
InterProi | View protein in InterPro IPR025721, Exosome_cplx_N_dom IPR026699, Exosome_RNA_bind1/RRP40/RRP4 IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR012340, NA-bd_OB-fold |
PANTHERi | PTHR21321, PTHR21321, 1 hit |
Pfami | View protein in Pfam PF14382, ECR1_N, 1 hit PF15985, KH_6, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF54791, SSF54791, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EXOS2_MOUSE | |
Accessioni | Q8VBV3Primary (citable) accession number: Q8VBV3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | March 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families