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Protein

Spike glycoprotein

Gene

S

Organism
Bovine coronavirus (strain 98TXSF-110-LUN) (BCoV-LUN) (BCV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.UniRule annotation
Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.UniRule annotation
Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.UniRule annotation

GO - Biological processi

Keywordsi

Biological processFusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virulence, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Spike glycoproteinUniRule annotation
Short name:
S glycoproteinUniRule annotation
Alternative name(s):
E2UniRule annotation
Peplomer proteinUniRule annotation
Cleaved into the following 3 chains:
Spike protein S1UniRule annotation
Spike protein S2UniRule annotation
Spike protein S2'UniRule annotation
Gene namesi
Name:SUniRule annotation
ORF Names:3
OrganismiBovine coronavirus (strain 98TXSF-110-LUN) (BCoV-LUN) (BCV)
Taxonomic identifieri233264 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000008571 Componenti: Genome

Subcellular locationi

  • Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host endoplasmic reticulum-Golgi intermediate compartment membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host cell membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Note: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini14 – 1307ExtracellularUniRule annotationAdd BLAST1294
Transmembranei1308 – 1328HelicalUniRule annotationAdd BLAST21
Topological domaini1329 – 1363CytoplasmicUniRule annotationAdd BLAST35

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 13UniRule annotationAdd BLAST13
ChainiPRO_000028391314 – 1363Spike glycoproteinAdd BLAST1350
ChainiPRO_000028391414 – 768Spike protein S1Add BLAST755
ChainiPRO_0000283915769 – 1363Spike protein S2Add BLAST595
ChainiPRO_0000444072914 – 1363Spike protein S2'UniRule annotationAdd BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi133N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi198N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi331 ↔ 356UniRule annotation
Glycosylationi359N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi374 ↔ 427UniRule annotation
Glycosylationi437N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi649N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi676N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi696N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi714N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi739N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi788N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi937N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi1194N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi1224N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi1234N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi1253N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi1267N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi1288N-linked (GlcNAc...) asparagine; by hostUniRule annotation1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. The precursor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins. Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor.UniRule annotation
The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei768 – 769Cleavage; by hostBy similarity2
Sitei913 – 914CleavageUniRule annotation2

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiQ8V436

Interactioni

Subunit structurei

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8V436
SMRiQ8V436
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni314 – 634Receptor-binding domainUniRule annotationAdd BLAST321
Regioni878 – 900Fusion peptideUniRule annotationAdd BLAST23
Regioni1014 – 1064Heptad repeat 1UniRule annotationAdd BLAST51
Regioni1258 – 1296Heptad repeat 2UniRule annotationAdd BLAST39

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1043 – 1087UniRule annotationAdd BLAST45
Coiled coili1269 – 1297UniRule annotationAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1359 – 1363KxHxxUniRule annotation5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1329 – 1346Cys-richAdd BLAST18

Sequence similaritiesi

Belongs to the betacoronaviruses spike protein family.UniRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG0900000Z

Family and domain databases

Gene3Di1.20.5.790, 1 hit
HAMAPiMF_04099 BETA_CORONA_SPIKE, 1 hit
InterProiView protein in InterPro
IPR002552 Corona_S2
IPR027400 S_HR2
IPR032500 Spike_N
IPR018548 Spike_rcpt-bd
IPR036326 Spike_rcpt-bd_sf
PfamiView protein in Pfam
PF01601 Corona_S2, 1 hit
PF16451 Spike_NTD, 1 hit
PF09408 Spike_rec_bind, 1 hit
SUPFAMiSSF143587 SSF143587, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8V436-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLILLISLP TAFAVIGDLK CTTVSINDVD TGVPSISTDT VDVTKGLGTY
60 70 80 90 100
YVLDRVYLNT TLLLNGYYPT SGSTYRNMAL KGTLLLSTLW FKPPFLSDFT
110 120 130 140 150
NGIFAKVKNT KVIKDGVMYS EFPAITIGST FVNTSYSVVV QPHTTILGNK
160 170 180 190 200
LQGFLEISVC QYTMCEYPNT ICNPNLGNRR VELWHWDTGV VSCLYKRNFT
210 220 230 240 250
YDVNADYLYF HFYQEGGTFY AYFTDTGVVT KFLFNVYLGT VLSHYYVMPL
260 270 280 290 300
TCNSALTLEY WVTPLTSKQY LLAFNQDGVI FNAVDCKSDF MSEIKCKTLS
310 320 330 340 350
IAPSTGVYEL NGYTVQPIAD VYRRIPNLPD CNIEAWLNDK SVPSPLNWER
360 370 380 390 400
KTFSNCNFNM SSLMSFIQAD SFTCNNIDAA KIYGMCFSSI TIDKFAIPNG
410 420 430 440 450
RKVDLQLGNL GYLQSFNYRI DTTATSCQLY YNLPAANVSV SRFNPSTWNR
460 470 480 490 500
RFGFTEQSVF KPQPAGVFTD HDVVYAQHCF KAPTNFCPCK LDGSLCVGNG
510 520 530 540 550
PGIDAGYKTS GIGTCPAGTN YLTCHNAAQC DCLCTPDPIT SKATGPYKCP
560 570 580 590 600
QTKYLVGIGE HCSGLAIKSD HCGGNPCTCQ PQAFLGWSVD SCLQGDRCNI
610 620 630 640 650
FANFILHDVN SGTTCSTDLQ KSNTDIILGV CVNYDLYGIT GQGIFVEVNA
660 670 680 690 700
TYYNSWQNLL YDSNGNLYGF RDYLTNRTFM IRSCYSGRVS AAFHANSSEP
710 720 730 740 750
ALLFRNIKCN YVFNNTLSRQ LQPINYFDSY LGCVVNADNS TSSVVQTCDL
760 770 780 790 800
TVGSGYCVDY STKRRSRRSI TTGYRFTNFE PFTVNSVNDS LEPVGGLYEI
810 820 830 840 850
QIPSEFTIGN MEEFIQTSSP KVTIDCSAFV CGDYAACKSQ LVEYGSFCDN
860 870 880 890 900
INAILTEVNE LLDTTQLQVA NSLMNGVTLS TKLKDGVNFN VDDINFSPVL
910 920 930 940 950
GCLGSDCNKV SSRSAIEDLL FSKVKLSDVG FVEAYNNCTG GAEIRDLICV
960 970 980 990 1000
QSYNGIKVLP PLLSENQISG YTLAATSASL FPPWSAAAGV PFYLNVQYRI
1010 1020 1030 1040 1050
NGIGVTMDVL SQNQKLIANA FNNALGAIQE GFDATNSALV KIQAVVNANA
1060 1070 1080 1090 1100
ETLNNLLQQL SNRFGAISSS LQEILSRLDA LEAQAQIDRL INGRLTALNA
1110 1120 1130 1140 1150
YVSQQLSDST LVKFSAAQAM EKVNECVKSQ SSRINFCGNG NHIISLVQNA
1160 1170 1180 1190 1200
PYGLYFIHFS YVPTKYVTAK VSPGLCIAGG RGIAPKSGYF VNVNNTWMFT
1210 1220 1230 1240 1250
GSGYYYPEPI TGNNVVVMST CAVNYTKAPD VMLNISTPNL PDFKEELDQW
1260 1270 1280 1290 1300
FKNQTSVAPD LSLDYINVTF LDLQDEMNRL QEAIKVLNQS YINLKDIGTY
1310 1320 1330 1340 1350
EYYVKWPWYV WLLIGFAGVA MLVLLFFICC CTGCGTSCFK KCGGCCDDYT
1360
GHQELVIKTS HED
Length:1,363
Mass (Da):150,543
Last modified:March 1, 2002 - v1
Checksum:i6824B55A588502D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF391542 Genomic RNA Translation: AAL57308.1

Similar proteinsi

Entry informationi

Entry nameiSPIKE_CVBLU
AccessioniPrimary (citable) accession number: Q8V436
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 1, 2002
Last modified: June 20, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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