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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]) (RV-A)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in stimulating the translation of viral mRNAs. These mRNAs are capped but not polyadenylated, instead terminating in a conserved sequence 'GACC' at the 3' that is recognized by NSP3, which competes with host PABPC1 for EIF4G1 binding. The interaction between NSP3 and host EIF4G1 stabilizes the EIF4E-EIF4G1 interaction, thereby facilitating the initiation of capped mRNA translation.UniRule annotation3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3UniRule annotation
Short name:
NSP3UniRule annotation
Alternative name(s):
NCVP4UniRule annotation
Non-structural RNA-binding protein 34UniRule annotation
Short name:
NS34UniRule annotation
OrganismiRotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]) (RV-A)
Taxonomic identifieri444185 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003678171 – 313Non-structural protein 3Add BLAST313

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1.UniRule annotationBy similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8UZL8
SMRiQ8UZL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149RNA-bindingUniRule annotationAdd BLAST149
Regioni150 – 206DimerizationUniRule annotationAdd BLAST57
Regioni170 – 234Interaction with host ZC3H7BUniRule annotationAdd BLAST65
Regioni208 – 313Interaction with host EIF4G1UniRule annotationAdd BLAST106

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili166 – 237UniRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the rotavirus NSP3 family.UniRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

HAMAPiMF_04094 ROTA_A_NSP3, 1 hit
MF_04090 ROTA_NSP3, 1 hit
InterProiView protein in InterPro
IPR036082 NSP3_sf
IPR002873 Rotavirus_NSP3
PfamiView protein in Pfam
PF01665 Rota_NSP3, 1 hit
SUPFAMiSSF69903 SSF69903, 1 hit

Sequencei

Sequence statusi: Complete.

Q8UZL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKMESTQQM ASSIINSSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF
60 70 80 90 100
DLVMDDSGVK NNLIGKAITI DQALNGKFSS AIRNRNWMTD SRTVAKLDED
110 120 130 140 150
VNKLRIMLSS KGIDQKMRVL NACFSVKRIP GKSSSIVKCT RLMKDKLERG
160 170 180 190 200
EVEVDDSFVE EKMEVDTIDW KSRYEQLEKR FESLKHRVNE KYNHWVLKAR
210 220 230 240 250
KVNENMNSLQ NVISQQQAHI NELQMYNNKL ERDLQSKIGS VVSSIEWYLR
260 270 280 290 300
SMELSDDVKS DIEQQLNSID QLNPVNAIDD FESILRNLIS DYDRLFIMFK
310
GLLQQCNYTY TYE
Length:313
Mass (Da):36,315
Last modified:March 1, 2002 - v1
Checksum:iC07F5AC22D8F5932
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065842 Genomic RNA Translation: AAL58536.1
DQ391186 Genomic RNA Translation: ABD52871.1

Similar proteinsi

Entry informationi

Entry nameiNSP3_ROTRH
AccessioniPrimary (citable) accession number: Q8UZL8
Secondary accession number(s): Q27PP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2002
Last modified: June 20, 2018
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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