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Protein

Phosphoribosyl-AMP cyclohydrolase

Gene

hisI

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.UniRule annotation

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi: L-histidine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG), ATP phosphoribosyltransferase regulatory subunit (hisZ)
  2. Phosphoribosyl-ATP pyrophosphatase (hisE)
  3. Phosphoribosyl-AMP cyclohydrolase (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. no protein annotated in this organism
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92MagnesiumUniRule annotation1
Metal bindingi93Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi94MagnesiumUniRule annotation1
Metal bindingi96MagnesiumUniRule annotation1
Metal bindingi111Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi118Zinc; shared with dimeric partnerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Histidine biosynthesis
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciAGRO:ATU1750-MONOMER
UniPathwayi
UPA00031;UER00008

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosyl-AMP cyclohydrolaseUniRule annotation (EC:3.5.4.19UniRule annotation)
Short name:
PRA-CHUniRule annotation
Gene namesi
Name:hisIUniRule annotation
Ordered Locus Names:Atu1750
ORF Names:AGR_C_3213
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001364561 – 150Phosphoribosyl-AMP cyclohydrolaseAdd BLAST150

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu1750

Structurei

3D structure databases

ProteinModelPortaliQ8UEK7
SMRiQ8UEK7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PRA-CH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K8F Bacteria
COG0139 LUCA
HOGENOMiHOG000277504
KOiK01496
OMAiTGYRSCF

Family and domain databases

Gene3Di3.10.20.400, 1 hit
HAMAPiMF_01021 HisI, 1 hit
InterProiView protein in InterPro
IPR026660 PRA-CH
IPR002496 PRib_AMP_CycHydrolase_dom
IPR038019 PRib_AMP_CycHydrolase_sf
PfamiView protein in Pfam
PF01502 PRA-CH, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002610 PRA_CycHdrlase, 1 hit
SUPFAMiSSF141734 SSF141734, 1 hit

Sequencei

Sequence statusi: Complete.

Q8UEK7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSIPFPSAPA DKEALENAGL FSPKFDAHGL VTAVVTDARD GELLMVAHMN
60 70 80 90 100
AEALSLTLET GIAHYYSRSR DKIWKKGETS GNLQTVKEFR TDCDQDAVWL
110 120 130 140 150
KVSVAGHDAT CHTGRRSCFY RTVELSNGDA VTKITDDTRH FDPATTYSNT
Length:150
Mass (Da):16,483
Last modified:May 27, 2002 - v1
Checksum:iA70554EE4F2799ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA Translation: AAK87520.2
PIRiAG2791
G97570
RefSeqiNP_354735.2, NC_003062.2
WP_010971841.1, NC_003062.2

Genome annotation databases

EnsemblBacteriaiAAK87520; AAK87520; Atu1750
GeneIDi1133788
KEGGiatu:Atu1750
PATRICifig|176299.10.peg.1763

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA Translation: AAK87520.2
PIRiAG2791
G97570
RefSeqiNP_354735.2, NC_003062.2
WP_010971841.1, NC_003062.2

3D structure databases

ProteinModelPortaliQ8UEK7
SMRiQ8UEK7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu1750

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK87520; AAK87520; Atu1750
GeneIDi1133788
KEGGiatu:Atu1750
PATRICifig|176299.10.peg.1763

Phylogenomic databases

eggNOGiENOG4105K8F Bacteria
COG0139 LUCA
HOGENOMiHOG000277504
KOiK01496
OMAiTGYRSCF

Enzyme and pathway databases

UniPathwayi
UPA00031;UER00008

BioCyciAGRO:ATU1750-MONOMER

Family and domain databases

Gene3Di3.10.20.400, 1 hit
HAMAPiMF_01021 HisI, 1 hit
InterProiView protein in InterPro
IPR026660 PRA-CH
IPR002496 PRib_AMP_CycHydrolase_dom
IPR038019 PRib_AMP_CycHydrolase_sf
PfamiView protein in Pfam
PF01502 PRA-CH, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002610 PRA_CycHdrlase, 1 hit
SUPFAMiSSF141734 SSF141734, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHIS3_AGRFC
AccessioniPrimary (citable) accession number: Q8UEK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: May 27, 2002
Last modified: October 10, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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