UniProtKB - Q8U4J3 (RFCS_PYRFU)
Protein
Replication factor C small subunit
Gene
rfcS
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Functioni
Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.2 Publications
Miscellaneous
The intein interrupts the potential ATP-binding site.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: InterPro
- endonuclease activity Source: InterPro
- four-way junction helicase activity Source: InterPro
GO - Biological processi
- DNA recombination Source: InterPro
- DNA repair Source: InterPro
- DNA replication Source: UniProtKB-KW
- intein-mediated protein splicing Source: InterPro
Keywordsi
Biological process | DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | PFUR186497:G1FZR-97-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Replication factor C small subunitShort name: RFC small subunit Alternative name(s): Clamp loader small subunit PfuRFC small subunit Cleaved into the following chain: |
Gene namesi | Name:rfcS Ordered Locus Names:PF0093 |
Organismi | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
Taxonomic identifieri | 186497 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 785 | E → A: Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-795; A-796; A-831 and A-835. 1 Publication | 1 | |
Mutagenesisi | 795 | E → A: Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-796; A-831 and A-835. 1 Publication | 1 | |
Mutagenesisi | 796 | D → A: Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-831 and A-835. 1 Publication | 1 | |
Mutagenesisi | 831 | E → A: Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-796 and A-835. 1 Publication | 1 | |
Mutagenesisi | 835 | E → A: Decreases the stability of the PCNA-RFC complex and reduces the clamp-loading activity; when associated with A-785; A-795; A-796 and A-831. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000030376 | 1 – 59 | Replication factor C small subunit, 1st partSequence analysisAdd BLAST | 59 | |
ChainiPRO_0000030377 | 60 – 584 | Pfu RFC inteinSequence analysisAdd BLAST | 525 | |
ChainiPRO_0000030378 | 585 – 852 | Replication factor C small subunit, 2nd partSequence analysisAdd BLAST | 268 |
Post-translational modificationi
This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated
Keywords - PTMi
Autocatalytic cleavage, Protein splicingProteomic databases
PRIDEi | Q8U4J3 |
Interactioni
Subunit structurei
Heteromultimer composed of three to four small subunits (RfcS) and one to two large subunits (RfcL).
1 PublicationProtein-protein interaction databases
STRINGi | 186497.PF0093 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8U4J3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8U4J3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 183 – 306 | DOD-type homing endonucleasePROSITE-ProRule annotationAdd BLAST | 124 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG00469, Archaea arCOG03154, Archaea |
HOGENOMi | CLU_015698_0_0_2 |
OMAi | DGHADSK |
OrthoDBi | 37207at2157 |
Family and domain databases
Gene3Di | 3.10.28.10, 1 hit |
InterProi | View protein in InterPro IPR003959, ATPase_AAA_core IPR008921, DNA_pol3_clamp-load_cplx_C IPR003586, Hint_dom_C IPR003587, Hint_dom_N IPR036844, Hint_dom_sf IPR027434, Homing_endonucl IPR006142, INTEIN IPR030934, Intein_C IPR004042, Intein_endonuc IPR006141, Intein_N IPR004860, LAGLIDADG_2 IPR027417, P-loop_NTPase IPR013748, Rep_factorC_C IPR008824, RuvB-like_N |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF14528, LAGLIDADG_3, 1 hit PF08542, Rep_fac_C, 1 hit PF05496, RuvB_N, 1 hit |
PRINTSi | PR00379, INTEIN |
SMARTi | View protein in SMART SM00305, HintC, 1 hit SM00306, HintN, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF51294, SSF51294, 1 hit SSF52540, SSF52540, 2 hits SSF55608, SSF55608, 1 hit |
TIGRFAMsi | TIGR01443, intein_Cterm, 1 hit TIGR01445, intein_Nterm, 1 hit |
PROSITEi | View protein in PROSITE PS50818, INTEIN_C_TER, 1 hit PS50819, INTEIN_ENDONUCLEASE, 1 hit PS50817, INTEIN_N_TER, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8U4J3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEEIREVKV LEKPWVEKYR PQRLDDIVGQ EHIVKRLKHY VKTGSMPHLL
60 70 80 90 100
FAGPPGVGKC LTGDTKVIAN GQLFELGELV EKLSGGRFGP TPVKGLKVLG
110 120 130 140 150
IDEDGKLREF EVQYVYKDRT DRLIKIKTQL GRELKVTPYH PLLVNRENGE
160 170 180 190 200
IKWIKAEELK PGDKLAIPSF LPLITGENPL AEWLGYFMGS GYAYPSNSVI
210 220 230 240 250
TFTNEDPLIR QRFMELTEKL FPDAKIRERI HADGTPEVYV VSRKAWSLVN
260 270 280 290 300
SISLTLIPRE GWKGIRSFLR AYSDCNGRIE SDAIVLSTDN NDMAQQIAYA
310 320 330 340 350
LASFGIIAKM DGEDVIISGS DNIERFLNEI GFSTQSKLKE AQKLIRKTNV
360 370 380 390 400
RSDGLKINYE LISYVKDRLR LNVNDKRNLS YRNAKELSWE LMKEIYYRLE
410 420 430 440 450
ELERLKKVLS EPILIDWNEV AKKSDEVIEK AKIRAEKLLE YIKGERKPSF
460 470 480 490 500
KEYIEIAKVL GINVERTIEA MKIFAKRYSS YAEIGRKLGT WNFNVKTILE
510 520 530 540 550
SDTVDNVEIL EKIRKIELEL IEEILSDGKL KEGIAYLIFL FQNELYWDEI
560 570 580 590 600
TEVKELRGDF IIYDLHVPGY HNFIAGNMPT VVHNTTAALA LARELFGENW
610 620 630 640 650
RHNFLELNAS DERGINVIRE KVKEFARTKP IGGASFKIIF LDEADALTQD
660 670 680 690 700
AQQALRRTME MFSSNVRFIL SCNYSSKIIE PIQSRCAIFR FRPLRDEDIA
710 720 730 740 750
KRLRYIAENE GLELTEEGLQ AILYIAEGDM RRAINILQAA AALDKKITDE
760 770 780 790 800
NVFMVASRAR PEDIREMMLL ALKGNFLKAR EKLREILLKQ GLSGEDVLVQ
810 820 830 840 850
MHKEVFNLPI EEPKKVLLAD KIGEYNFRLV EGANEIIQLE ALLAQFTLIG
KK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE009950 Genomic DNA Translation: AAL80217.1 AB037375 Genomic DNA Translation: BAB03291.1 AB037375 Genomic DNA Translation: BAB03292.1 |
RefSeqi | WP_011011205.1, NZ_CP023154.1 |
Genome annotation databases
EnsemblBacteriai | AAL80217; AAL80217; PF0093 |
GeneIDi | 41711880 |
KEGGi | pfu:PF0093 |
PATRICi | fig|186497.12.peg.97 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE009950 Genomic DNA Translation: AAL80217.1 AB037375 Genomic DNA Translation: BAB03291.1 AB037375 Genomic DNA Translation: BAB03292.1 |
RefSeqi | WP_011011205.1, NZ_CP023154.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1IQP | X-ray | 2.80 | A/B/C/D/E/F | 1-59 | [»] | |
A/B/C/D/E/F | 585-852 | [»] | ||||
SMRi | Q8U4J3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 186497.PF0093 |
Proteomic databases
PRIDEi | Q8U4J3 |
Genome annotation databases
EnsemblBacteriai | AAL80217; AAL80217; PF0093 |
GeneIDi | 41711880 |
KEGGi | pfu:PF0093 |
PATRICi | fig|186497.12.peg.97 |
Phylogenomic databases
eggNOGi | arCOG00469, Archaea arCOG03154, Archaea |
HOGENOMi | CLU_015698_0_0_2 |
OMAi | DGHADSK |
OrthoDBi | 37207at2157 |
Enzyme and pathway databases
BioCyci | PFUR186497:G1FZR-97-MONOMER |
Miscellaneous databases
EvolutionaryTracei | Q8U4J3 |
Family and domain databases
Gene3Di | 3.10.28.10, 1 hit |
InterProi | View protein in InterPro IPR003959, ATPase_AAA_core IPR008921, DNA_pol3_clamp-load_cplx_C IPR003586, Hint_dom_C IPR003587, Hint_dom_N IPR036844, Hint_dom_sf IPR027434, Homing_endonucl IPR006142, INTEIN IPR030934, Intein_C IPR004042, Intein_endonuc IPR006141, Intein_N IPR004860, LAGLIDADG_2 IPR027417, P-loop_NTPase IPR013748, Rep_factorC_C IPR008824, RuvB-like_N |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF14528, LAGLIDADG_3, 1 hit PF08542, Rep_fac_C, 1 hit PF05496, RuvB_N, 1 hit |
PRINTSi | PR00379, INTEIN |
SMARTi | View protein in SMART SM00305, HintC, 1 hit SM00306, HintN, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF51294, SSF51294, 1 hit SSF52540, SSF52540, 2 hits SSF55608, SSF55608, 1 hit |
TIGRFAMsi | TIGR01443, intein_Cterm, 1 hit TIGR01445, intein_Nterm, 1 hit |
PROSITEi | View protein in PROSITE PS50818, INTEIN_C_TER, 1 hit PS50819, INTEIN_ENDONUCLEASE, 1 hit PS50817, INTEIN_N_TER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFCS_PYRFU | |
Accessioni | Q8U4J3Primary (citable) accession number: Q8U4J3 Secondary accession number(s): Q9P9H2, Q9P9H3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 16, 2004 |
Last sequence update: | June 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Intein-containing proteins
List of intein-containing protein entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families