Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thermostable carboxypeptidase 1

Gene

PF0456

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues, but not Pro, Gly, Asp and Glu.1 Publication

Catalytic activityi

Release of a C-terminal amino acid with broad specificity, except for -Pro.1 Publication

Cofactori

Co2+2 Publications, Mn2+2 PublicationsNote: Binds 1 cobalt ion per subunit. Can also utilize Mn2+ (in vitro). Is not active with zinc ions.2 Publications

Activity regulationi

EDTA and DTT reversibly abolish carboxypeptidase activity.1 Publication

pH dependencei

Optimum pH is 6.2-6.6.1 Publication

Temperature dependencei

Optimum temperature is 90-100 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi269Cobalt; catalytic1 Publication1
Active sitei270Proton donor/acceptorBy similarity1
Metal bindingi273Cobalt; catalytic1 Publication1
Metal bindingi299Cobalt1 Publication1

GO - Molecular functioni

  • cobalt ion binding Source: UniProtKB
  • metallocarboxypeptidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandCobalt, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.17.B5 5243

Protein family/group databases

MEROPSiM32.002

Names & Taxonomyi

Protein namesi
Recommended name:
Thermostable carboxypeptidase 1 (EC:3.4.17.19)
Alternative name(s):
Carboxypeptidase Pfu
Short name:
PfuCP
Gene namesi
Ordered Locus Names:PF0456
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004288341 – 499Thermostable carboxypeptidase 1Add BLAST499

Proteomic databases

PRIDEiQ8U3L0

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi186497.PF0456

Structurei

Secondary structure

1499
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8U3L0
SMRiQ8U3L0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U3L0

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M32 family.Curated

Phylogenomic databases

eggNOGiarCOG04247 Archaea
COG2317 LUCA
HOGENOMiHOG000040803
KOiK01299
OMAiEFGHALY
OrthoDBiPOG093Z01FT

Family and domain databases

CDDicd06460 M32_Taq, 1 hit
InterProiView protein in InterPro
IPR001333 Peptidase_M32_Taq
PANTHERiPTHR34217 PTHR34217, 1 hit
PfamiView protein in Pfam
PF02074 Peptidase_M32, 1 hit
PIRSFiPIRSF006615 Zn_crbxpep_Taq, 1 hit
PRINTSiPR00998 CRBOXYPTASET

Sequencei

Sequence statusi: Complete.

Q8U3L0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEVFQNETI KQILAKYRRI WAIGHAQSVL GWDLEVNMPK EGILERSVAQ
60 70 80 90 100
GELSVLSHEL LLHPEFVNLV EKAKGLENLN EYERGIVRVL DRSIRIARAF
110 120 130 140 150
PPEFIREVSE TTSLATKAWE EAKAKDDFSK FEPWLDKIIS LAKRAAEYLG
160 170 180 190 200
YEEEPYDALL DLYEEGLRTR DVEKMFEVLE KKLKPLLDKI LEEGKVPREH
210 220 230 240 250
PLEKEKYERE WMERVNLWIL QKFGFPLGTR ARLDVSAHPF TTEFGIRDVR
260 270 280 290 300
ITTRYEGYDF RRTILSTVHE FGHALYELQQ DERFMFTPIA GGVSLGIHES
310 320 330 340 350
QSRFWENIIG RSKEFVELIY PVLKENLPFM SNYTPEDVYL YFNIVRPDFI
360 370 380 390 400
RTEADVVTYN FHILLRFKLE RLMVSEEIKA KDLPEMWNDE MERLLGIRPR
410 420 430 440 450
KYSEGILQDI HWAHGSIGYF PTYTIGTLLS AQLYYHIKKD IPDFEEKVAK
460 470 480 490
AEFDPIKAWL REKIHRWGSI YPPKELLKKA IGEDMDAEYF VRWVKEKYL
Length:499
Mass (Da):59,043
Last modified:June 1, 2002 - v1
Checksum:i627A8347CD899BC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA Translation: AAL80580.1
RefSeqiWP_011011573.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL80580; AAL80580; PF0456
GeneIDi1468297
KEGGipfu:PF0456
PATRICifig|186497.12.peg.480

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA Translation: AAL80580.1
RefSeqiWP_011011573.1, NC_003413.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K9XX-ray2.30A/B/C/D1-499[»]
1KA2X-ray2.20A1-499[»]
1KA4X-ray3.00A1-499[»]
ProteinModelPortaliQ8U3L0
SMRiQ8U3L0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0456

Protein family/group databases

MEROPSiM32.002

Proteomic databases

PRIDEiQ8U3L0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80580; AAL80580; PF0456
GeneIDi1468297
KEGGipfu:PF0456
PATRICifig|186497.12.peg.480

Phylogenomic databases

eggNOGiarCOG04247 Archaea
COG2317 LUCA
HOGENOMiHOG000040803
KOiK01299
OMAiEFGHALY
OrthoDBiPOG093Z01FT

Enzyme and pathway databases

BRENDAi3.4.17.B5 5243

Miscellaneous databases

EvolutionaryTraceiQ8U3L0

Family and domain databases

CDDicd06460 M32_Taq, 1 hit
InterProiView protein in InterPro
IPR001333 Peptidase_M32_Taq
PANTHERiPTHR34217 PTHR34217, 1 hit
PfamiView protein in Pfam
PF02074 Peptidase_M32, 1 hit
PIRSFiPIRSF006615 Zn_crbxpep_Taq, 1 hit
PRINTSiPR00998 CRBOXYPTASET
ProtoNetiSearch...

Entry informationi

Entry nameiCBP1_PYRFU
AccessioniPrimary (citable) accession number: Q8U3L0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: June 1, 2002
Last modified: September 12, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again