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Entry version 91 (08 May 2019)
Sequence version 1 (01 Jun 2002)
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Protein

Acetate--CoA ligase [ADP-forming] II subunit alpha

Gene

acdAII

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reversible formation of acetate and ATP from acetyl-CoA by using ADP and phosphate. Can use other substrates such as phenylacetyl-CoA, indoleacetyl-CoA and isobutyryl-CoA, but not succinyl-CoA. Seems to be involved primarily in the degradation of aryl-CoA esters to the corresponding acids. Participates in the conversion of acetyl-CoA to acetate and in the degradation of branched-chain amino acids via branched-chain-acyl-CoA esters.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 115 sec(-1) for ADP. kcat is 21 sec(-1) for GDP. kcat is 117 sec(-1) for phosphate. kcat is 42 sec(-1) for acetyl-CoA. kcat is 8 sec(-1) for isobutyryl-CoA. kcat is 138 sec(-1) for phenylacetyl-CoA. kcat is 68 sec(-1) for ATP. kcat is 27 sec(-1) for GTP. kcat is 70 sec(-1) for CoA. kcat is 67 sec(-1) for acetate. kcat is 22 sec(-1) for isobutyrate. kcat is 66 sec(-1) for indoleacetate. kcat is 89 sec(-1) for phenylacetate.1 Publication
  1. KM=61 µM for ADP (at 80 degrees Celsius)1 Publication
  2. KM=236 µM for GDP (at 80 degrees Celsius)1 Publication
  3. KM=580 µM for phosphate (at 80 degrees Celsius)1 Publication
  4. KM=26 µM for acetyl-CoA (at 80 degrees Celsius)1 Publication
  5. KM=12 µM for isobutyryl-CoA (at 80 degrees Celsius)1 Publication
  6. KM=4 µM for phenylacetyl-CoA (at 80 degrees Celsius)1 Publication
  7. KM=326 µM for ATP (at 80 degrees Celsius)1 Publication
  8. KM=770 µM for GTP (at 80 degrees Celsius)1 Publication
  9. KM=74 µM for CoA (at 80 degrees Celsius)1 Publication
  10. KM=10700 µM for acetate (at 80 degrees Celsius)1 Publication
  11. KM=5800 µM for isobutyrate (at 80 degrees Celsius)1 Publication
  12. KM=2000 µM for indoleacetate (at 80 degrees Celsius)1 Publication
  13. KM=768 µM for phenylacetate (at 80 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 9.0 (at 80 degrees Celsius).1 Publication

    Temperature dependencei

    Optimum temperature is above 90 degrees Celsius (at pH 8.0).1 Publication

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionLigase
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    PFUR186497:G1FZR-556-MONOMER

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    Q8U3D6

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Acetate--CoA ligase [ADP-forming] II subunit alphaCurated (EC:6.2.1.131 Publication)
    Alternative name(s):
    ADP-forming acetyl coenzyme A synthetase II subunit alphaCurated
    Short name:
    ACS II subunit alphaCurated
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:acdAII1 Publication
    Ordered Locus Names:PF0532Imported
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri186497 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001013 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004305221 – 457Acetate--CoA ligase [ADP-forming] II subunit alphaAdd BLAST457

    Proteomic databases

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q8U3D6

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Heterotetramer of two alpha and two beta subunits.

    1 Publication

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    186497.PF0532

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q8U3D6

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    arCOG01340 Archaea
    COG1042 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000220089

    KEGG Orthology (KO)

    More...
    KOi
    K01905

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    ICVVPTF

    Database of Orthologous Groups

    More...
    OrthoDBi
    15044at2157

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.40.50.261, 2 hits

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR014089 AcCoA-synth-alpha
    IPR003781 CoA-bd
    IPR036291 NAD(P)-bd_dom_sf
    IPR032875 Succ_CoA_lig_flav_dom
    IPR016102 Succinyl-CoA_synth-like

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF13380 CoA_binding_2, 1 hit
    PF13607 Succ_CoA_lig, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00881 CoA_binding, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF51735 SSF51735, 1 hit
    SSF52210 SSF52210, 2 hits

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR02717 AcCoA-syn-alpha, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    Q8U3D6-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MLDYFFNPRG IAVIGASNDP KKLGYEVFKN LKEYQGGKVY PVNVREEEVQ
    60 70 80 90 100
    GVKAYKSVKE IPGEVDLAII VVPKKFVKQT LIECGEKGVK GVVIITAGFG
    110 120 130 140 150
    ETGEEGKREE KELVEIAHKY GMRIIGPNCV GIMNTHANLN ATFITVAKKG
    160 170 180 190 200
    NVAFISQSGA LGAGIVYKTI KEDIGFSKFI SVGNMADLDF ADLMEYLADT
    210 220 230 240 250
    QEDKAIALYI EGIKDGRRFI EVAKKVTKKK PVIALKAGKS ESGSRAAASH
    260 270 280 290 300
    TGSLAGSWKI YEAAFKQSGV LVANTIDEML SMARAFTQPL PKGNRVAIMT
    310 320 330 340 350
    NAGGPGVLTA DEIDKRGLKL ANLEEKTIEE LRSFLPPMAA VKNPVDMIAS
    360 370 380 390 400
    ARGEDYYRTA KLLLQDPNVD ILIAICVVPT FAGMTPTEHA EGIIRAVKEV
    410 420 430 440 450
    NNGKPVLALF MAGYVSEKAK ELLEKNGIPT YERPEDVAAA AYALVQQAKN

    VGGGVNG
    Length:457
    Mass (Da):49,263
    Last modified:June 1, 2002 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF28D69E0412EC53A
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AE009950 Genomic DNA Translation: AAL80656.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_011011649.1, NC_003413.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAL80656; AAL80656; PF0532

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    1468373

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    pfu:PF0532

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|186497.12.peg.556

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE009950 Genomic DNA Translation: AAL80656.1
    RefSeqiWP_011011649.1, NC_003413.1

    3D structure databases

    SMRiQ8U3D6
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi186497.PF0532

    Proteomic databases

    PRIDEiQ8U3D6

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL80656; AAL80656; PF0532
    GeneIDi1468373
    KEGGipfu:PF0532
    PATRICifig|186497.12.peg.556

    Phylogenomic databases

    eggNOGiarCOG01340 Archaea
    COG1042 LUCA
    HOGENOMiHOG000220089
    KOiK01905
    OMAiICVVPTF
    OrthoDBi15044at2157

    Enzyme and pathway databases

    BioCyciPFUR186497:G1FZR-556-MONOMER
    SABIO-RKiQ8U3D6

    Family and domain databases

    Gene3Di3.40.50.261, 2 hits
    InterProiView protein in InterPro
    IPR014089 AcCoA-synth-alpha
    IPR003781 CoA-bd
    IPR036291 NAD(P)-bd_dom_sf
    IPR032875 Succ_CoA_lig_flav_dom
    IPR016102 Succinyl-CoA_synth-like
    PfamiView protein in Pfam
    PF13380 CoA_binding_2, 1 hit
    PF13607 Succ_CoA_lig, 1 hit
    SMARTiView protein in SMART
    SM00881 CoA_binding, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    SSF52210 SSF52210, 2 hits
    TIGRFAMsiTIGR02717 AcCoA-syn-alpha, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiACDA2_PYRFU
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8U3D6
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
    Last sequence update: June 1, 2002
    Last modified: May 8, 2019
    This is version 91 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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