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Entry version 111 (07 Apr 2021)
Sequence version 1 (01 Jun 2002)
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Protein

NAD(P)H sulfur oxidoreductase (CoA-dependent)

Gene

nsr

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the CoA-dependent reduction of elemental sulfur (S0) to produce hydrogen sulfide (PubMed:17449625, PubMed:24723088).

Can use both NADPH and NADH, but shows a preference for NADPH (PubMed:24723088).

May enable S0 to be used, via sulfide, for iron-sulfur cluster synthesis by SipA (Probable). Also shows coenzyme A disulfide reductase (CoADR) activity with both NADH and NADPH (PubMed:15720393, PubMed:17449625).

However, CoADR specific activity is about 20-fold lower than the sulfur reduction assay and CoADR activity appears to be an artifactual side reaction and is not thought to have any physiological relevance (PubMed:17449625).

Also shows NAD(P)H oxidase activity with both NADH and NADPH (PubMed:24723088).

1 Publication3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

FAD2 PublicationsNote: Binds 1 FAD per subunit.By similarity

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=8.5 mM for NADPH1 Publication
  2. KM=3.3 mM for NADH1 Publication
  3. KM=18 µM for CoA1 Publication
  4. KM=10 µM for CoA-disulfide1 Publication

    pH dependencei

    Optimum pH is 6.5 for sulfide production.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei24Coenzyme ABy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei45Redox-activeBy similarity1
    Binding sitei72Coenzyme ABy similarity1
    Binding sitei82FAD; via amide nitrogen and carbonyl oxygenBy similarity1
    Binding sitei280FADBy similarity1
    Binding sitei298FAD; via amide nitrogenBy similarity1
    Binding sitei302Coenzyme ABy similarity1
    Binding sitei358Coenzyme A; via carbonyl oxygenBy similarity1
    Binding sitei422FAD; via carbonyl oxygenBy similarity1
    Binding sitei430Coenzyme ABy similarity1
    Binding sitei438Coenzyme ABy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi13 – 14FADBy similarity2
    Nucleotide bindingi35 – 36FADBy similarity2
    Nucleotide bindingi42 – 44FADBy similarity3

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MetaCyc:MONOMER-18049
    PFUR186497:G1FZR-1220-MONOMER

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    1.8.1.18, 5243

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    NAD(P)H sulfur oxidoreductase (CoA-dependent)Curated (EC:1.8.1.181 Publication)
    Short name:
    NSR1 Publication
    Alternative name(s):
    Coenzyme A disulfide reductase1 Publication (EC:1.8.1.141 Publication)
    Short name:
    CoA-disulfide reductaseCurated
    Short name:
    CoADR1 Publication
    NAD(P)H oxidaseCurated (EC:1.6.3.-1 Publication)
    Short name:
    NOX1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:nsr1 Publication
    Ordered Locus Names:PF1186
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri186497 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001013 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Deletion mutant does not exhibit an obvious phenotype and produces sulfide and acetate in amounts comparable to those produced by the parent strain.1 Publication

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001846981 – 442NAD(P)H sulfur oxidoreductase (CoA-dependent)Add BLAST442

    Proteomic databases

    PRoteomics IDEntifications database

    More...
    PRIDEi
    Q8U1M0

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    Up-regulated up to sevenfold by elemental sulfur addition.1 Publication

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    2 Publications

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    186497.PF1186

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    Q8U1M0

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni41 – 45Coenzyme A bindingBy similarity5
    Regioni62 – 63Coenzyme A bindingBy similarity2

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    arCOG01069, Archaea

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_003291_1_3_2

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    WVSHAPC

    Database of Orthologous Groups

    More...
    OrthoDBi
    40511at2157

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.50.50.60, 3 hits

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR017758, CoA_disulphide_reductase
    IPR036188, FAD/NAD-bd_sf
    IPR023753, FAD/NAD-binding_dom
    IPR016156, FAD/NAD-linked_Rdtase_dimer_sf
    IPR004099, Pyr_nucl-diS_OxRdtase_dimer

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF07992, Pyr_redox_2, 1 hit
    PF02852, Pyr_redox_dim, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF51905, SSF51905, 1 hit
    SSF55424, SSF55424, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR03385, CoA_CoA_reduc, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    Q8U1M0-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MEKKKVVIIG GGAAGMSAAS RVKRLRPEWD VKVFEATEWV SHAPCGIPYV
    60 70 80 90 100
    VEGIAPKEKL MHYPPEVFIK KRGIDLHLKA EVIEVETGYV RVRENGEEKS
    110 120 130 140 150
    YEWDYLVFAN GASPQIPEIE GVDLPGVFTA DLPPDAVAIT EYMEKNKVED
    160 170 180 190 200
    VVIIGTGYIA LEMAEAFVTR GKNVTLIGRS ERVLRKTFDK EITDIVEEKL
    210 220 230 240 250
    RQHLNLRLHE KTLSIEGRER VEKVITDGGE YKADLVIIAT GIKPNVELAK
    260 270 280 290 300
    QLGVKIGETG AIWTNEKMQT SVENVYAAGD VAETKHVITG KRVWIPLAPA
    310 320 330 340 350
    GNKMGYVAGS NIAGKEIEFP GVLGTSITKF MDLEIGKTGL TENEAVKEGY
    360 370 380 390 400
    DVRTAFIKAN TKPHYYPGGR EIWLKGVVDN ETNRLLGVQA VGAEILPRID
    410 420 430 440
    SAAAMITAGF TTKDVFFTDL AYAPPFAPVW DPLIVLARVL KF
    Length:442
    Mass (Da):48,720
    Last modified:June 1, 2002 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2329E5F596F1CA6A
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AE009950 Genomic DNA Translation: AAL81310.1

    NCBI Reference Sequences

    More...
    RefSeqi
    WP_011012327.1, NZ_CP023154.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAL81310; AAL81310; PF1186

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    41712995

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    pfu:PF1186

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|186497.12.peg.1247

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE009950 Genomic DNA Translation: AAL81310.1
    RefSeqiWP_011012327.1, NZ_CP023154.1

    3D structure databases

    SMRiQ8U1M0
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi186497.PF1186

    Proteomic databases

    PRIDEiQ8U1M0

    Genome annotation databases

    EnsemblBacteriaiAAL81310; AAL81310; PF1186
    GeneIDi41712995
    KEGGipfu:PF1186
    PATRICifig|186497.12.peg.1247

    Phylogenomic databases

    eggNOGiarCOG01069, Archaea
    HOGENOMiCLU_003291_1_3_2
    OMAiWVSHAPC
    OrthoDBi40511at2157

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18049
    PFUR186497:G1FZR-1220-MONOMER
    BRENDAi1.8.1.18, 5243

    Family and domain databases

    Gene3Di3.50.50.60, 3 hits
    InterProiView protein in InterPro
    IPR017758, CoA_disulphide_reductase
    IPR036188, FAD/NAD-bd_sf
    IPR023753, FAD/NAD-binding_dom
    IPR016156, FAD/NAD-linked_Rdtase_dimer_sf
    IPR004099, Pyr_nucl-diS_OxRdtase_dimer
    PfamiView protein in Pfam
    PF07992, Pyr_redox_2, 1 hit
    PF02852, Pyr_redox_dim, 1 hit
    SUPFAMiSSF51905, SSF51905, 1 hit
    SSF55424, SSF55424, 1 hit
    TIGRFAMsiTIGR03385, CoA_CoA_reduc, 1 hit

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNSR_PYRFU
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8U1M0
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
    Last sequence update: June 1, 2002
    Last modified: April 7, 2021
    This is version 111 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
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