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Protein

Uridylate kinase

Gene

pyrH

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor.1 Publication

Catalytic activityi

ATP + UMP = ADP + UDP.

Activity regulationi

Inhibited by UTP.By similarity

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UDP from UMP (UMPK route).
Proteins known to be involved in this subpathway in this organism are:
  1. Uridylate kinase (pyrH)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP from UMP (UMPK route), the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi6Magnesium 11 Publication1
Binding sitei44UMP; via amide nitrogen1 Publication1
Binding sitei45ATP; via amide nitrogen1
Binding sitei49ATPBy similarity1
Binding sitei66UMP1 Publication1
Metal bindingi120Magnesium 11 Publication1
Metal bindingi121Magnesium 11 Publication1
Metal bindingi121Magnesium 21 Publication1
Binding sitei140ATP1
Binding sitei141ATP1
Binding sitei146ATP; via amide nitrogen and carbonyl oxygen1
Binding sitei149ATP1
Binding sitei179UMP; via amide nitrogen1 Publication1
Metal bindingi182Magnesium 2; via carbonyl oxygen1 Publication1
Binding sitei182ATP; via amide nitrogen1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 10ATP2
Nucleotide bindingi114 – 120UMP1 Publication7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPyrimidine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPFUR186497:G1FZR-1442-MONOMER
BRENDAi2.7.4.22 5243
UniPathwayi
UPA00159;UER00275

Names & Taxonomyi

Protein namesi
Recommended name:
Uridylate kinase (EC:2.7.4.22)
Short name:
UK
Alternative name(s):
Uridine monophosphate kinase
Short name:
UMP kinase
Short name:
UMPK
Gene namesi
Name:pyrH
Ordered Locus Names:PF1407
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001439231 – 225Uridylate kinaseAdd BLAST225

Proteomic databases

PRIDEiQ8U122

Interactioni

Subunit structurei

Homohexamer; trimer of dimers.2 Publications

Protein-protein interaction databases

STRINGi186497.PF1407

Structurei

Secondary structure

1225
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8U122
SMRiQ8U122
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8U122

Family & Domainsi

Sequence similaritiesi

Belongs to the UMP kinase family.Curated

Phylogenomic databases

eggNOGiarCOG00858 Archaea
COG0528 LUCA
HOGENOMiHOG000047188
KOiK09903
OMAiMGGTHPG
OrthoDBiPOG093Z0AM3

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_01220_A PyrH_A, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR011817 Uridylate_kinase
IPR011818 Uridylate_kinase_arch/spir
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF005650 Uridylate_kin, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR02076 pyrH_arch, 1 hit

Sequencei

Sequence statusi: Complete.

Q8U122-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRIVFDIGGS VLVPENPDID FIKEIAYQLT KVSEDHEVAV VVGGGKLARK
60 70 80 90 100
YIEVAEKFNS SETFKDFIGI QITRANAMLL IAALREKAYP VVVEDFWEAW
110 120 130 140 150
KAVQLKKIPV MGGTHPGHTT DAVAALLAEF LKADLLVVIT NVDGVYTADP
160 170 180 190 200
KKDPTAKKIK KMKPEELLEI VGKGIEKAGS SSVIDPLAAK IIARSGIKTI
210 220
VIGKEDAKDL FRVIKGDHNG TTIEP
Length:225
Mass (Da):24,491
Last modified:June 1, 2002 - v1
Checksum:i510D151A02F8D534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA Translation: AAL81531.1
RefSeqiWP_011012554.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL81531; AAL81531; PF1407
GeneIDi1469283
KEGGipfu:PF1407
PATRICifig|186497.12.peg.1470

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA Translation: AAL81531.1
RefSeqiWP_011012554.1, NC_003413.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BMUX-ray2.55A/B1-225[»]
2BRIX-ray3.00A/B1-225[»]
2BRXX-ray2.40A/B1-225[»]
2JI5X-ray2.45A/B1-225[»]
ProteinModelPortaliQ8U122
SMRiQ8U122
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1407

Proteomic databases

PRIDEiQ8U122

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81531; AAL81531; PF1407
GeneIDi1469283
KEGGipfu:PF1407
PATRICifig|186497.12.peg.1470

Phylogenomic databases

eggNOGiarCOG00858 Archaea
COG0528 LUCA
HOGENOMiHOG000047188
KOiK09903
OMAiMGGTHPG
OrthoDBiPOG093Z0AM3

Enzyme and pathway databases

UniPathwayi
UPA00159;UER00275

BioCyciPFUR186497:G1FZR-1442-MONOMER
BRENDAi2.7.4.22 5243

Miscellaneous databases

EvolutionaryTraceiQ8U122

Family and domain databases

Gene3Di3.40.1160.10, 1 hit
HAMAPiMF_01220_A PyrH_A, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR011817 Uridylate_kinase
IPR011818 Uridylate_kinase_arch/spir
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PIRSFiPIRSF005650 Uridylate_kin, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
TIGRFAMsiTIGR02076 pyrH_arch, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPYRH_PYRFU
AccessioniPrimary (citable) accession number: Q8U122
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: June 1, 2002
Last modified: September 12, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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