We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - Q8TZR3 (PIMT_PYRFU)
Protein
Protein-L-isoaspartate O-methyltransferase
Gene
pcm
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Functioni
Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins (By similarity).
By similarityCatalytic activityi
- [protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate α-methyl ester + S-adenosyl-L-homocysteineEC:2.1.1.77
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 65 | By similarity | 1 |
GO - Molecular functioni
- protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- protein repair Source: UniProtKB-UniRule
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.77, 5243 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-L-isoaspartate O-methyltransferase (EC:2.1.1.77)Alternative name(s): L-isoaspartyl protein carboxyl methyltransferase Protein L-isoaspartyl methyltransferase Protein-beta-aspartate methyltransferase Short name: PIMT |
Gene namesi | Name:pcm Ordered Locus Names:PF1922 |
Organismi | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) |
Taxonomic identifieri | 186497 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000111923 | 1 – 219 | Protein-L-isoaspartate O-methyltransferaseAdd BLAST | 219 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q8TZR3 |
SMRi | Q8TZR3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8TZR3 |
Family & Domainsi
Sequence similaritiesi
Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.Curated
Phylogenomic databases
eggNOGi | arCOG00976, Archaea |
HOGENOMi | CLU_055432_2_0_2 |
OMAi | TISAIHM |
PhylomeDBi | Q8TZR3 |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
HAMAPi | MF_00090, PIMT, 1 hit |
InterProi | View protein in InterPro IPR000682, PCMT IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11579, PTHR11579, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00080, pimt, 1 hit |
PROSITEi | View protein in PROSITE PS01279, PCMT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8TZR3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMDEKELYEK WMRTVEMLKA EGIIRSKEVE RAFLKYPRYL FVEDKYKKYA
60 70 80 90 100
HIDEPLPIPA GQTVSAPHMV AIMLEIANLK PGMNILEVGT GSGWNAALIS
110 120 130 140 150
EIVKTDVYTI ERIPELVEFA KRNLERAGVK NVHVILGDGS KGFPPKAPYD
160 170 180 190 200
VIIVTAGAPK IPEPLIEQLK IGGKLIIPVG SYHLWQELLE VRKTKDGIKI
210
KNHGGVAFVP LIGEYGWKE
Sequence cautioni
The sequence AAL82046 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE009950 Genomic DNA Translation: AAL82046.1 Different initiation. |
Genome annotation databases
EnsemblBacteriai | AAL82046; AAL82046; PF1922 |
KEGGi | pfu:PF1922 |
PATRICi | fig|186497.12.peg.1993 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE009950 Genomic DNA Translation: AAL82046.1 Different initiation. |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JG1 | X-ray | 1.20 | A | 1-219 | [»] | |
1JG2 | X-ray | 1.50 | A | 1-219 | [»] | |
1JG3 | X-ray | 2.10 | A/B | 1-219 | [»] | |
1JG4 | X-ray | 1.50 | A | 1-219 | [»] | |
AlphaFoldDBi | Q8TZR3 | |||||
SMRi | Q8TZR3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 186497.PF1922 |
Genome annotation databases
EnsemblBacteriai | AAL82046; AAL82046; PF1922 |
KEGGi | pfu:PF1922 |
PATRICi | fig|186497.12.peg.1993 |
Phylogenomic databases
eggNOGi | arCOG00976, Archaea |
HOGENOMi | CLU_055432_2_0_2 |
OMAi | TISAIHM |
PhylomeDBi | Q8TZR3 |
Enzyme and pathway databases
BRENDAi | 2.1.1.77, 5243 |
Miscellaneous databases
EvolutionaryTracei | Q8TZR3 |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
HAMAPi | MF_00090, PIMT, 1 hit |
InterProi | View protein in InterPro IPR000682, PCMT IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11579, PTHR11579, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00080, pimt, 1 hit |
PROSITEi | View protein in PROSITE PS01279, PCMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PIMT_PYRFU | |
Accessioni | Q8TZR3Primary (citable) accession number: Q8TZR3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2002 |
Last sequence update: | October 10, 2002 | |
Last modified: | May 25, 2022 | |
This is version 108 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families