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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NAD; via amide nitrogenUniRule annotation1
Binding sitei60NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei124NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi152Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi167IronUniRule annotation1
Binding sitei210NAD; via amide nitrogenUniRule annotation1
Binding sitei220GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 53NADUniRule annotation2
Nucleotide bindingi150 – 152NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

BioCyciMKAN190192:G139I-441-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:MK0430Imported
OrganismiMethanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)Imported
Taxonomic identifieri190192 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanopyriMethanopyralesMethanopyraceaeMethanopyrus
Proteomesi
  • UP000001826 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi190192.MK0430

Structurei

3D structure databases

ProteinModelPortaliQ8TY75
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TY75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEREITPIVL REGYEFINDC SESDVIVVGA GPAGLTCAYE LAKSDVDVTI
60 70 80 90 100
VERKLYVGGG MTGGGMLFPA GVIMEETAEV LEEVGVELRP AEAGLLAFNP
110 120 130 140 150
VEAAIKLANA ALEAGARILV GIEVEDVIER RGRVCGVVVN WTAVKAANMH
160 170 180 190 200
VDPLALEAEY TVDATGHEAA VCKLAGIEVK GEGPMWAERG EELVVKHTQE
210 220 230 240
VKPGLFVAGM AASAVKGAYR MGPIFGGMLE SGKKAAEEIL ERLTE
Length:245
Mass (Da):25,869
Last modified:June 1, 2002 - v1
Checksum:i8943D49DF1315885
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009439 Genomic DNA Translation: AAM01645.1

Genome annotation databases

EnsemblBacteriaiAAM01645; AAM01645; MK0430
KEGGimka:MK0430
PATRICifig|190192.8.peg.459

Similar proteinsi

Entry informationi

Entry nameiQ8TY75_METKA
AccessioniPrimary (citable) accession number: Q8TY75
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: July 18, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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