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Entry version 117 (02 Dec 2020)
Sequence version 1 (01 Jun 2002)
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Protein

Ion-translocating oxidoreductase complex subunit B

Gene

rnfB

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Part of a membrane-bound complex that couples electron transfer with translocation of ions across the membrane. Catalyzes Na+ transport, most probably coupled to electron transfer from reduced ferredoxin to methanophenazine and heterodisulfide reductase. Involved in heterodisulfide reduction during methanogenesis from acetate.1 Publication

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterUniRule annotation1 PublicationNote: Binds 5 [4Fe-4S] clusters.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi50Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi58Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi75Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi138Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi142Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi148Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi152Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi172Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi175Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi178Iron-sulfur 3 (4Fe-4S)UniRule annotation1
Metal bindingi182Iron-sulfur 2 (4Fe-4S)UniRule annotation1
Metal bindingi217Iron-sulfur 4 (4Fe-4S)UniRule annotation1
Metal bindingi220Iron-sulfur 4 (4Fe-4S)UniRule annotation1
Metal bindingi223Iron-sulfur 4 (4Fe-4S)UniRule annotation1
Metal bindingi227Iron-sulfur 4 (4Fe-4S)UniRule annotation1
Metal bindingi246Iron-sulfur 5 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi249Iron-sulfur 5 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi252Iron-sulfur 5 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi256Iron-sulfur 5 (4Fe-4S)PROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTranslocase
Biological processElectron transport, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MACE188937:G1FZT-706-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
3.D.6.1.3, the ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ion-translocating oxidoreductase complex subunit BUniRule annotationCurated (EC:7.2.1.-UniRule annotationCurated)
Alternative name(s):
Rnf electron transport complex subunit BUniRule annotationCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:rnfBUniRule annotation
Ordered Locus Names:MA_0664Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri188937 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiArchaeaEuryarchaeotaStenosarchaea groupMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002487 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei5 – 25HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Deletion of the rnf operon abolishes growth on acetate and ferredoxin:heterodisulfide oxidoreductase-coupled Na+ transport.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004435191 – 264Ion-translocating oxidoreductase complex subunit BAdd BLAST264

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

The Rnf complex is probably composed of eight subunits, including RnfA, RnfB, RnfC, RnfD, RnfE and RnfG.

UniRule annotation1 Publication

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q8TSX9

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini33 – 924Fe-4SUniRule annotationAdd BLAST60
Domaini127 – 1624Fe-4S ferredoxin-type 1UniRule annotationAdd BLAST36
Domaini163 – 1924Fe-4S ferredoxin-type 2UniRule annotationAdd BLAST30
Domaini207 – 2364Fe-4S ferredoxin-type 3UniRule annotationAdd BLAST30
Domaini237 – 2644Fe-4S ferredoxin-type 4UniRule annotationAdd BLAST28

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily.UniRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_053470_0_0_2

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q8TSX9

Identification of Orthologs from Complete Genome Data

More...
OMAi
MLPGANC

Database of Orthologous Groups

More...
OrthoDBi
111853at2157

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q8TSX9

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
3.20.20.20, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00463, RsxB_RnfB, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR007202, 4Fe-4S_dom
IPR017896, 4Fe4S_Fe-S-bd
IPR017900, 4Fe4S_Fe_S_CS
IPR011005, Dihydropteroate_synth-like
IPR010207, Elect_transpt_cplx_RnfB/RsxB

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00037, Fer4, 1 hit
PF13187, Fer4_9, 1 hit
PF04060, FeS, 1 hit

TIGRFAMs; a protein family database

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TIGRFAMsi
TIGR01944, rnfB, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00197, 2FE2S_FER_1, 1 hit
PS51656, 4FE4S, 1 hit
PS00198, 4FE4S_FER_1, 3 hits
PS51379, 4FE4S_FER_2, 4 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q8TSX9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSVLINSIA VLAGLGFAVG VMLVIASKVF KIDSNPLIDD VASLLPGANC
60 70 80 90 100
GGCGFAGCAA CAEAIVEQGA PVNSCPVGGF EVAKQIGALL GQEVTESEKE
110 120 130 140 150
FPFVRCQGGN QHCTTLYDYH GVENCKVALM LCDSRKGCTY GCLGLGTCVQ
160 170 180 190 200
ACQFGALSMG EDGFPVVNKA LCTSCGNCIA ACPNGVLTFA RDSEKVHVLC
210 220 230 240 250
RSHDKGKDVK AVCEVGCIGC KKCEKECPAG AIRVTEFLAE IDQEKCTACG
260
ACVAICPQKA IELR
Length:264
Mass (Da):27,352
Last modified:June 1, 2002 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i693976388A3BEEDD
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM04106.1

NCBI Reference Sequences

More...
RefSeqi
WP_011020711.1, NC_003552.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAM04106; AAM04106; MA_0664

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
1472556

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mac:MA_0664

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM04106.1
RefSeqiWP_011020711.1, NC_003552.1

3D structure databases

SMRiQ8TSX9
ModBaseiSearch...

Protein family/group databases

TCDBi3.D.6.1.3, the ion (h(+) or na(+))-translocating nadh:ferredoxin oxidoreductase (nfo or rnf) family

Genome annotation databases

EnsemblBacteriaiAAM04106; AAM04106; MA_0664
GeneIDi1472556
KEGGimac:MA_0664

Phylogenomic databases

HOGENOMiCLU_053470_0_0_2
InParanoidiQ8TSX9
OMAiMLPGANC
OrthoDBi111853at2157
PhylomeDBiQ8TSX9

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-706-MONOMER

Family and domain databases

Gene3Di3.20.20.20, 1 hit
HAMAPiMF_00463, RsxB_RnfB, 1 hit
InterProiView protein in InterPro
IPR007202, 4Fe-4S_dom
IPR017896, 4Fe4S_Fe-S-bd
IPR017900, 4Fe4S_Fe_S_CS
IPR011005, Dihydropteroate_synth-like
IPR010207, Elect_transpt_cplx_RnfB/RsxB
PfamiView protein in Pfam
PF00037, Fer4, 1 hit
PF13187, Fer4_9, 1 hit
PF04060, FeS, 1 hit
TIGRFAMsiTIGR01944, rnfB, 1 hit
PROSITEiView protein in PROSITE
PS00197, 2FE2S_FER_1, 1 hit
PS51656, 4FE4S, 1 hit
PS00198, 4FE4S_FER_1, 3 hits
PS51379, 4FE4S_FER_2, 4 hits

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRNFB_METAC
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8TSX9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2018
Last sequence update: June 1, 2002
Last modified: December 2, 2020
This is version 117 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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