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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144Zinc 1UniRule annotation1
Metal bindingi147Zinc 1UniRule annotation1
Metal bindingi161Zinc 2UniRule annotation1
Metal bindingi164Zinc 2UniRule annotation1
Metal bindingi187Zinc 2UniRule annotation1
Metal bindingi190Zinc 2UniRule annotation1
Metal bindingi201Zinc 1UniRule annotation1
Metal bindingi204Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri131 – 213CR-typeUniRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-1560-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:MA_1479
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000709481 – 382Chaperone protein DnaJAdd BLAST382

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi188937.MA1479

Structurei

3D structure databases

ProteinModelPortaliQ8TQR1
SMRiQ8TQR1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 70JUniRule annotationAdd BLAST65
Repeati144 – 151CXXCXGXG motif8
Repeati161 – 168CXXCXGXG motif8
Repeati187 – 194CXXCXGXG motif8
Repeati201 – 208CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 114Gly-richAdd BLAST40

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri131 – 213CR-typeUniRule annotationAdd BLAST83

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiarCOG02846 Archaea
COG0484 LUCA
InParanoidiQ8TQR1
KOiK03686
OMAiDMGGFAD
OrthoDBiPOG093Z045I
PhylomeDBiQ8TQR1

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
HAMAPiMF_01152 DnaJ, 1 hit
InterProiView protein in InterPro
IPR012724 DnaJ
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 3 hits
SSF57938 SSF57938, 1 hit
TIGRFAMsiTIGR02349 DnaJ_bact, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS51188 ZF_CR, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TQR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKRDYYEI LGLPKDASVE DIKKTYRKLA LQYHPDRNKD PGAEDKFKEI
60 70 80 90 100
SEAYAVLSDT EKRAQYDRFG HAGIDNQYSA EDIFRGADFG GFGDIFEMFF
110 120 130 140 150
GGGRRGGPMG PRRGSDLQYD LYITFEEAAF GVRKDIDIPR TERCSTCSGT
160 170 180 190 200
GAKPGTSPKR CPTCGGTGQV RTTRSTLGMQ FISTTTCSTC HGRGQIIESP
210 220 230 240 250
CPVCGGAGRV RNKRTITVNV PAGADSGMSL RLSGEGDSGE PGAPSGDLYI
260 270 280 290 300
IIHVMEHRHF KRVDYDVISE LSITFTQAAL GADVMVDTLY GKVKMNIPAG
310 320 330 340 350
TQTHSVFRLR DKGIQRLHGH GKGDQLVRVI IKTPTKLNQE QKELLRQFEN
360 370 380
LSKGKKPQEE EKSKAEKHKK GIFEKVKDAF ES
Length:382
Mass (Da):42,203
Last modified:June 1, 2002 - v1
Checksum:i2875C8AC1CE9C9B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM04893.1
RefSeqiWP_011021493.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM04893; AAM04893; MA_1479
GeneIDi1473367
KEGGimac:MA_1479

Similar proteinsi

Entry informationi

Entry nameiDNAJ_METAC
AccessioniPrimary (citable) accession number: Q8TQR1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2002
Last modified: February 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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