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UniProtKB - Q8TMG0 (ICMT_METAC)
Protein
Protein-S-isoprenylcysteine O-methyltransferase
Gene
MA_2698
Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Functioni
Carboxyl methyltransferase with activity toward prenyl lipids. In vitro, displays activity toward N-acetyl-S-farnesyl-L-cysteine (AFC) and S-farnesylthioacetic acid (FTA), an analog of AFC.
1 PublicationCatalytic activityi
- [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1001 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 108 | S-adenosyl-L-methionine; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 121 | S-adenosyl-L-methionine1 Publication | 1 | |
Binding sitei | 163 | SubstrateBy similarity | 1 | |
Binding sitei | 167 | S-adenosyl-L-methionine1 Publication | 1 |
GO - Molecular functioni
- protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-S-isoprenylcysteine O-methyltransferaseCurated (EC:2.1.1.1001 Publication)Alternative name(s): Isoprenylcysteine carboxyl methyltransferase1 Publication Short name: ICMT1 Publication |
Gene namesi | Ordered Locus Names:MA_2698Imported |
Organismi | Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) |
Taxonomic identifieri | 188937 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Stenosarchaea group › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 | Extracellular1 Publication | 1 | |
Transmembranei | 2 – 28 | Helical1 PublicationAdd BLAST | 27 | |
Topological domaini | 29 – 37 | Cytoplasmic1 Publication | 9 | |
Transmembranei | 38 – 62 | Helical1 PublicationAdd BLAST | 25 | |
Topological domaini | 63 – 73 | Extracellular1 PublicationAdd BLAST | 11 | |
Transmembranei | 74 – 98 | Helical1 PublicationAdd BLAST | 25 | |
Topological domaini | 99 – 125 | Cytoplasmic1 PublicationAdd BLAST | 27 | |
Transmembranei | 126 – 144 | Helical1 PublicationAdd BLAST | 19 | |
Topological domaini | 145 | Extracellular1 Publication | 1 | |
Transmembranei | 146 – 166 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 167 – 194 | Cytoplasmic1 PublicationAdd BLAST | 28 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 113 | H → A: Abolishes FTA methylation. 1 Publication | 1 | |
Mutagenesisi | 126 | H → A: Abolishes FTA methylation. 1 Publication | 1 | |
Mutagenesisi | 163 | R → A: Abolishes FTA methylation. 1 Publication | 1 | |
Mutagenesisi | 167 | E → A: Abolishes FTA methylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000449360 | 1 – 194 | Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST | 194 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q8TMG0 |
SMRi | Q8TMG0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 113 – 116 | S-adenosyl-L-methionine binding1 Publication | 4 | |
Regioni | 126 – 129 | S-adenosyl-L-methionine binding1 Publication | 4 |
Sequence similaritiesi
Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
HOGENOMi | CLU_065200_1_1_2 |
InParanoidi | Q8TMG0 |
OMAi | FSLWLFR |
OrthoDBi | 94584at2157 |
PhylomeDBi | Q8TMG0 |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8TMG0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNENLWKICF IVMFIIWVFV RKVYGTRAMK NKSKKKVRPN FEKSLVFLNF
60 70 80 90 100
IGMVFLPLTA VFSSYLDSFN INLPDSIRLF ALIVTFLNIG LFTKIHKDLG
110 120 130 140 150
NNWSAILEIK DGHKLVKEGI YKNIRHPMYA HLWLWVITQG IILSNWVVLI
160 170 180 190
FGIVAWAILY FIRVPKEEEL LIEEFGDEYI EYMGKTGRLF PKVV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE010299 Genomic DNA Translation: AAM06077.1 |
RefSeqi | WP_011022658.1, NC_003552.1 |
Genome annotation databases
EnsemblBacteriai | AAM06077; AAM06077; MA_2698 |
GeneIDi | 1474591 |
KEGGi | mac:MA_2698 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE010299 Genomic DNA Translation: AAM06077.1 |
RefSeqi | WP_011022658.1, NC_003552.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4A2N | X-ray | 3.40 | B | 1-194 | [»] | |
AlphaFoldDBi | Q8TMG0 | |||||
SMRi | Q8TMG0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
EnsemblBacteriai | AAM06077; AAM06077; MA_2698 |
GeneIDi | 1474591 |
KEGGi | mac:MA_2698 |
Phylogenomic databases
HOGENOMi | CLU_065200_1_1_2 |
InParanoidi | Q8TMG0 |
OMAi | FSLWLFR |
OrthoDBi | 94584at2157 |
PhylomeDBi | Q8TMG0 |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ICMT_METAC | |
Accessioni | Q8TMG0Primary (citable) accession number: Q8TMG0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 22, 2020 |
Last sequence update: | June 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 93 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families