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Protein

Probable tRNA-splicing endonuclease subunit sen2

Gene

sen2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei281By similarity1
Active sitei289By similarity1
Active sitei325By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-splicing endonuclease subunit sen2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease sen2
Gene namesi
Name:sen2
ORF Names:SPAPB17E12.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAPB17E12.07c
PomBaseiSPAPB17E12.07c sen2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094611 – 380Probable tRNA-splicing endonuclease subunit sen2Add BLAST380

Proteomic databases

MaxQBiQ8TFH7
PaxDbiQ8TFH7
PRIDEiQ8TFH7

Interactioni

Subunit structurei

Heterotetramer composed of sen2, sen15, sen34 and sen54. Interacts directly with sen54 (By similarity).By similarity

Protein-protein interaction databases

STRINGi4896.SPAPB17E12.07c.1

Structurei

3D structure databases

ProteinModelPortaliQ8TFH7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

HOGENOMiHOG000159660
InParanoidiQ8TFH7
KOiK15322
OMAiWLHCVNR
OrthoDBiEOG092C2YVE
PhylomeDBiQ8TFH7

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006676 tRNA_splic
IPR016589 tRNA_splic_SEN2
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PIRSFiPIRSF011789 tRNA_splic_SEN2, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TFH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKNHEVYKD ALPISLAYPL PPIILTNPLT WIPYIYRYLF RKTPRQVQWQ
60 70 80 90 100
CQLHESDLSC VVTDSEAIKK FWTSGFFGKG NLSRSEPTWH TRTKRSLGLL
110 120 130 140 150
GFDEDLVAEE VTARRRFQRK QFKAQRAYRE NRARERQLLL ENGKPIPASL
160 170 180 190 200
EEDAELPEYL TKSLKDFSRV SENPYHITSV PNVEHLQLTF PEAFFLASLG
210 220 230 240 250
VLRINYENPN FELLPILKLF ANIVANSVAL THDYSLQQSH EDPIIEPDNK
260 270 280 290 300
FLTELAAYFY FRQQGWVVKN GTKFSVDFLL YKKGPVFSHA EFAILLIPCV
310 320 330 340 350
GNKQKYNMQW HEVHCLNRVI AQVKKSLILC YVQCPSIEDF NKIWKNQASM
360 370 380
NEWDWAESVL RQYLIRCVTL RRWVPSRNRD
Length:380
Mass (Da):44,608
Last modified:June 1, 2002 - v1
Checksum:iE458D08F0D920249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAD27500.1
RefSeqiNP_001018222.1, NM_001018702.2

Genome annotation databases

EnsemblFungiiSPAPB17E12.07c.1; SPAPB17E12.07c.1:pep; SPAPB17E12.07c
GeneIDi3361398
KEGGispo:SPAPB17E12.07c

Similar proteinsi

Entry informationi

Entry nameiSEN2_SCHPO
AccessioniPrimary (citable) accession number: Q8TFH7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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