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Protein

Serine/threonine-protein kinase haspin

Gene

HASPIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+2 Publications

Activity regulationi

Constitutive activity that does not require phosphorylation. Specifically inhibited by 3-(1H-indazol-5-yl)-N-propylimidazo[1,2-b]pyridazin-6-amine (CHR-6494).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei511ATP1
Active sitei649Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi490 – 498ATPPROSITE-ProRule annotationBy similarity9
Nucleotide bindingi606 – 611ATP6
Nucleotide bindingi649 – 654ATP6
Nucleotide bindingi687 – 689ATP3

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • histone kinase activity (H3-T3 specific) Source: UniProtKB
  • protein kinase activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ8TF76

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase haspin (EC:2.7.11.1)
Alternative name(s):
Germ cell-specific gene 2 protein
H-haspin
Haploid germ cell-specific nuclear protein kinase
Gene namesi
Name:HASPINImported
Synonyms:GSG2Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000177602.5
HGNCiHGNC:19682 HASPIN
MIMi609240 gene
neXtProtiNX_Q8TF76

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi492E → A: Markedly reduced affinity for histone H3 and reduced histone H3 phosphorylation. 1 Publication1
Mutagenesisi511K → A: Strongly reduced enzyme activity. 1 Publication1
Mutagenesisi651H → A: Strongly reduced enzyme activity, markedly reduced affinity for histone H3. 2 Publications1
Mutagenesisi707D → L: Markedly reduced affinity for histone H3 and reduced histone H3 phosphorylation. 1 Publication1
Mutagenesisi709D → N: Markedly reduced affinity for histone H3 and reduced histone H3 phosphorylation. 1 Publication1
Mutagenesisi713G → F: Markedly reduced affinity for histone H3 and reduced histone H3 phosphorylation. 1 Publication1
Mutagenesisi716D → L: Markedly reduced histone H3 phosphorylation. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000177602
PharmGKBiPA134909705

Chemistry databases

ChEMBLiCHEMBL1075163
DrugBankiDB04604 5-iodotubercidin
DB07995 N-[2-(4-BROMOCINNAMYLAMINO)ETHYL]-5-ISOQUINOLINE SULFONAMIDE
GuidetoPHARMACOLOGYi2028

Polymorphism and mutation databases

BioMutaiGSG2
DMDMi296439330

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859891 – 798Serine/threonine-protein kinase haspinAdd BLAST798

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei58PhosphoserineCombined sources1
Modified residuei93Phosphoserine; by AURKBCombined sources1 Publication1
Modified residuei97PhosphothreonineCombined sources1
Modified residuei143Phosphoserine; by AURKB1 Publication1
Modified residuei147PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated on both serine and threonine residues (By similarity). Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis.By similarity2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TF76
MaxQBiQ8TF76
PaxDbiQ8TF76
PeptideAtlasiQ8TF76
PRIDEiQ8TF76
ProteomicsDBi74576
74577 [Q8TF76-2]

PTM databases

iPTMnetiQ8TF76
PhosphoSitePlusiQ8TF76

Expressioni

Tissue specificityi

Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.2 Publications

Gene expression databases

BgeeiENSG00000177602 Expressed in 79 organ(s), highest expression level in secondary oocyte
CleanExiHS_GSG2
GenevisibleiQ8TF76 HS

Organism-specific databases

HPAiHPA027422
HPA030698

Interactioni

Binary interactionsi

Protein-protein interaction databases

BioGridi123814, 34 interactors
DIPiDIP-38174N
IntActiQ8TF76, 12 interactors
MINTiQ8TF76
STRINGi9606.ENSP00000325290

Chemistry databases

BindingDBiQ8TF76

Structurei

Secondary structure

1798
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8TF76
SMRiQ8TF76
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TF76

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini484 – 798Protein kinasePROSITE-ProRule annotationCuratedAdd BLAST315

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2464 Eukaryota
COG5072 LUCA
GeneTreeiENSGT00390000013015
HOGENOMiHOG000168256
HOVERGENiHBG049009
InParanoidiQ8TF76
KOiK16315
OMAiLCQHSLF
OrthoDBiEOG091G07NN
PhylomeDBiQ8TF76
TreeFamiTF313895

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 12 Publications (identifier: Q8TF76-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAASLPGPGS RLFRTYGAAD GRRQRRPGRE AAQWFPPQDR RRFFNSSGSS
60 70 80 90 100
DASIGDPSQS DDPDDPDDPD FPGSPVRRRR RRPGGRVPKD RPSLTVTPKR
110 120 130 140 150
WKLRARPSLT VTPRRLGLRA RPPQKCSTPC GPLRLPPFPS RDSGRLSPDL
160 170 180 190 200
SVCGQPRDGD ELGISASLFS SLASPCPGSP TPRDSVISIG TSACLVAASA
210 220 230 240 250
VPSGLHLPEV SLDRASLPCS QEEATGGAKD TRMVHQTRAS LRSVLFGLMN
260 270 280 290 300
SGTPEDSEFR ADGKNMRESC CKRKLVVGNG PEGPGLSSTG KRRATGQDSC
310 320 330 340 350
QERGLQEAVR REHQEASVPK GRIVPRGIDR LERTRSSRKS KHQEATETSL
360 370 380 390 400
LHSHRFKKGQ KLGKDSFPTQ DLTPLQNVCF WTKTRASFSF HKKKIVTDVS
410 420 430 440 450
EVCSIYTTAT SLSGSLLSEC SNRPVMNRTS GAPSSWHSSS MYLLSPLNTL
460 470 480 490 500
SISNKKASDA EKVYGECSQK GPVPFSHCLP TEKLQRCEKI GEGVFGEVFQ
510 520 530 540 550
TIADHTPVAI KIIAIEGPDL VNGSHQKTFE EILPEIIISK ELSLLSGEVC
560 570 580 590 600
NRTEGFIGLN SVHCVQGSYP PLLLKAWDHY NSTKGSANDR PDFFKDDQLF
610 620 630 640 650
IVLEFEFGGI DLEQMRTKLS SLATAKSILH QLTASLAVAE ASLRFEHRDL
660 670 680 690 700
HWGNVLLKKT SLKKLHYTLN GKSSTIPSCG LQVSIIDYTL SRLERDGIVV
710 720 730 740 750
FCDVSMDEDL FTGDGDYQFD IYRLMKKENN NRWGEYHPYS NVLWLHYLTD
760 770 780 790
KMLKQMTFKT KCNTPAMKQI KRKIQEFHRT MLNFSSATDL LCQHSLFK
Length:798
Mass (Da):88,495
Last modified:May 18, 2010 - v3
Checksum:iE0EDF8F0C58ADA31
GO
Isoform 21 Publication (identifier: Q8TF76-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     314-314: Q → H
     315-798: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:314
Mass (Da):33,850
Checksum:i7F8CC086036ED24E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti273R → S in BAB71255 (PubMed:14702039).Curated1
Sequence conflicti339K → E in AAH47457 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04054076V → E1 PublicationCorresponds to variant dbSNP:rs11653889Ensembl.1
Natural variantiVAR_04054182R → C4 PublicationsCorresponds to variant dbSNP:rs9907144Ensembl.1
Natural variantiVAR_040542145R → H1 PublicationCorresponds to variant dbSNP:rs55991903Ensembl.1
Natural variantiVAR_027405204G → D3 PublicationsCorresponds to variant dbSNP:rs220462Ensembl.1
Natural variantiVAR_040543283G → S1 PublicationCorresponds to variant dbSNP:rs56224301Ensembl.1
Natural variantiVAR_040544301Q → L1 PublicationCorresponds to variant dbSNP:rs55649477Ensembl.1
Natural variantiVAR_027406328I → T3 PublicationsCorresponds to variant dbSNP:rs220461Ensembl.1
Natural variantiVAR_027407378V → A3 PublicationsCorresponds to variant dbSNP:rs3809806Ensembl.1
Natural variantiVAR_027408422N → D. Corresponds to variant dbSNP:rs7223226Ensembl.1
Natural variantiVAR_040545706M → V1 PublicationCorresponds to variant dbSNP:rs56134695Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050671314Q → H in isoform 2. 1 Publication1
Alternative sequenceiVSP_050672315 – 798Missing in isoform 2. 1 PublicationAdd BLAST484

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039834 mRNA Translation: BAB21938.3
AF289865 mRNA Translation: AAK30300.1
AK056691 mRNA Translation: BAB71255.1
AC116914 Genomic DNA No translation available.
BC047457 mRNA Translation: AAH47457.1
CCDSiCCDS11036.1 [Q8TF76-1]
RefSeqiNP_114171.2, NM_031965.2 [Q8TF76-1]
UniGeneiHs.534059

Genome annotation databases

EnsembliENST00000325418; ENSP00000325290; ENSG00000177602 [Q8TF76-1]
GeneIDi83903
KEGGihsa:83903
UCSCiuc002fwp.4 human [Q8TF76-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039834 mRNA Translation: BAB21938.3
AF289865 mRNA Translation: AAK30300.1
AK056691 mRNA Translation: BAB71255.1
AC116914 Genomic DNA No translation available.
BC047457 mRNA Translation: AAH47457.1
CCDSiCCDS11036.1 [Q8TF76-1]
RefSeqiNP_114171.2, NM_031965.2 [Q8TF76-1]
UniGeneiHs.534059

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VUWX-ray1.80A465-798[»]
2WB8X-ray2.15A452-798[»]
3DLZX-ray1.85A465-798[»]
3E7VX-ray2.00A471-798[»]
3F2NX-ray1.80A465-798[»]
3FMDX-ray2.00A470-798[»]
3IQ7X-ray2.00A465-798[»]
4OUCX-ray1.90A465-798[»]
4QTCX-ray1.40A465-798[»]
5HTBX-ray1.70A465-798[»]
5HTCX-ray1.50A465-798[»]
6G34X-ray1.76A465-798[»]
6G35X-ray1.55A465-798[»]
6G36X-ray1.46A465-798[»]
6G37X-ray1.48A465-798[»]
6G38X-ray1.47A465-798[»]
6G39X-ray1.45A465-798[»]
6G3AX-ray1.43A465-798[»]
ProteinModelPortaliQ8TF76
SMRiQ8TF76
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123814, 34 interactors
DIPiDIP-38174N
IntActiQ8TF76, 12 interactors
MINTiQ8TF76
STRINGi9606.ENSP00000325290

Chemistry databases

BindingDBiQ8TF76
ChEMBLiCHEMBL1075163
DrugBankiDB04604 5-iodotubercidin
DB07995 N-[2-(4-BROMOCINNAMYLAMINO)ETHYL]-5-ISOQUINOLINE SULFONAMIDE
GuidetoPHARMACOLOGYi2028

PTM databases

iPTMnetiQ8TF76
PhosphoSitePlusiQ8TF76

Polymorphism and mutation databases

BioMutaiGSG2
DMDMi296439330

Proteomic databases

EPDiQ8TF76
MaxQBiQ8TF76
PaxDbiQ8TF76
PeptideAtlasiQ8TF76
PRIDEiQ8TF76
ProteomicsDBi74576
74577 [Q8TF76-2]

Protocols and materials databases

DNASUi83903
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325418; ENSP00000325290; ENSG00000177602 [Q8TF76-1]
GeneIDi83903
KEGGihsa:83903
UCSCiuc002fwp.4 human [Q8TF76-1]

Organism-specific databases

CTDi83903
EuPathDBiHostDB:ENSG00000177602.5
GeneCardsiHASPIN
H-InvDBiHIX0013433
HGNCiHGNC:19682 HASPIN
HPAiHPA027422
HPA030698
MIMi609240 gene
neXtProtiNX_Q8TF76
OpenTargetsiENSG00000177602
PharmGKBiPA134909705
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2464 Eukaryota
COG5072 LUCA
GeneTreeiENSGT00390000013015
HOGENOMiHOG000168256
HOVERGENiHBG049009
InParanoidiQ8TF76
KOiK16315
OMAiLCQHSLF
OrthoDBiEOG091G07NN
PhylomeDBiQ8TF76
TreeFamiTF313895

Enzyme and pathway databases

SignaLinkiQ8TF76

Miscellaneous databases

EvolutionaryTraceiQ8TF76
GeneWikiiGSG2
GenomeRNAii83903
PROiPR:Q8TF76
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000177602 Expressed in 79 organ(s), highest expression level in secondary oocyte
CleanExiHS_GSG2
GenevisibleiQ8TF76 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHASP_HUMAN
AccessioniPrimary (citable) accession number: Q8TF76
Secondary accession number(s): Q5U5K3, Q96MN1, Q9BXS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 18, 2010
Last modified: November 7, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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