UniProtKB - Q8TF42 (UBS3B_HUMAN)
Ubiquitin-associated and SH3 domain-containing protein B
UBASH3B
Functioni
Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.
2 PublicationsCatalytic activityi
- EC:3.1.3.481 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 390 | By similarity | 1 | |
Active sitei | 391 | Tele-phosphohistidine intermediateBy similarity | 1 | |
Active sitei | 576 | By similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- phosphoprotein binding Source: Ensembl
- protein tyrosine phosphatase activity Source: GO_Central
- ubiquitin protein ligase binding Source: Ensembl
GO - Biological processi
- collagen-activated tyrosine kinase receptor signaling pathway Source: GO_Central
- negative regulation of bone resorption Source: GO_Central
- negative regulation of osteoclast differentiation Source: Ensembl
- negative regulation of platelet aggregation Source: Ensembl
- negative regulation of protein kinase activity Source: GO_Central
- negative regulation of signal transduction Source: GO_Central
- peptidyl-tyrosine dephosphorylation Source: GO_Central
- platelet aggregation Source: GO_Central
- regulation of osteoclast differentiation Source: GO_Central
- regulation of protein binding Source: Ensembl
- regulation of release of sequestered calcium ion into cytosol Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Enzyme and pathway databases
PathwayCommonsi | Q8TF42 |
SignaLinki | Q8TF42 |
SIGNORi | Q8TF42 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-associated and SH3 domain-containing protein B (EC:3.1.3.48)Alternative name(s): Cbl-interacting protein p70 Suppressor of T-cell receptor signaling 1 Short name: STS-1 T-cell ubiquitin ligand 2 Short name: TULA-2 Tyrosine-protein phosphatase STS1/TULA2 |
Gene namesi | Name:UBASH3B Synonyms:KIAA1959, STS1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29884, UBASH3B |
MIMi | 609201, gene |
neXtProti | NX_Q8TF42 |
VEuPathDBi | HostDB:ENSG00000154127 |
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 84959 |
OpenTargetsi | ENSG00000154127 |
PharmGKBi | PA162407840 |
Miscellaneous databases
Pharosi | Q8TF42, Tbio |
Genetic variation databases
BioMutai | UBASH3B |
DMDMi | 110287974 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000245508 | 1 – 649 | Ubiquitin-associated and SH3 domain-containing protein BAdd BLAST | 649 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Modified residuei | 23 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8TF42 |
jPOSTi | Q8TF42 |
MassIVEi | Q8TF42 |
MaxQBi | Q8TF42 |
PaxDbi | Q8TF42 |
PeptideAtlasi | Q8TF42 |
PRIDEi | Q8TF42 |
ProteomicsDBi | 74552 |
PTM databases
DEPODi | UBASH3B |
GlyGeni | Q8TF42, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q8TF42 |
PhosphoSitePlusi | Q8TF42 |
Expressioni
Gene expression databases
Bgeei | ENSG00000154127, Expressed in cerebellum and 174 other tissues |
Genevisiblei | Q8TF42, HS |
Organism-specific databases
HPAi | ENSG00000154127, Tissue enhanced (brain, lymphoid tissue, placenta) |
Interactioni
Subunit structurei
Homodimer.
Interacts with JAK2 (in vitro) (By similarity).
Interacts with CBL (PubMed:15159412).
Part of a complex containing CBL and activated EGFR (PubMed:15159412).
Interacts with ubiquitin and with mono-ubiquitinated proteins (PubMed:15159412).
Interacts with ZAP70 (ubiquitinated form) (PubMed:26903241).
By similarity2 PublicationsBinary interactionsi
Q8TF42
GO - Molecular functioni
- identical protein binding Source: IntAct
- phosphoprotein binding Source: Ensembl
- ubiquitin protein ligase binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 124390, 144 interactors |
IntActi | Q8TF42, 65 interactors |
MINTi | Q8TF42 |
STRINGi | 9606.ENSP00000284273 |
Miscellaneous databases
RNActi | Q8TF42, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q8TF42 |
SMRi | Q8TF42 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8TF42 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 76 | UBAPROSITE-ProRule annotationAdd BLAST | 50 | |
Domaini | 254 – 319 | SH3PROSITE-ProRule annotationAdd BLAST | 66 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 380 – 649 | Protein tyrosine phosphataseBy similarityAdd BLAST | 270 |
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG3734, Eukaryota |
GeneTreei | ENSGT00940000156097 |
HOGENOMi | CLU_016516_1_0_1 |
InParanoidi | Q8TF42 |
OMAi | DECGTWV |
OrthoDBi | 243749at2759 |
PhylomeDBi | Q8TF42 |
TreeFami | TF313334 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit cd11936, SH3_UBASH3B, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR015940, UBA IPR009060, UBA-like_sf IPR035632, UBASH3B_SH3 |
Pfami | View protein in Pfam PF00300, His_Phos_1, 1 hit PF14604, SH3_9, 1 hit PF00627, UBA, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit SM00165, UBA, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF50044, SSF50044, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit PS50030, UBA, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAQYGHPSPL GMAAREELYS KVTPRRNRQQ RPGTIKHGSA LDVLLSMGFP
60 70 80 90 100
RARAQKALAS TGGRSVQAAC DWLFSHVGDP FLDDPLPREY VLYLRPTGPL
110 120 130 140 150
AQKLSDFWQQ SKQICGKNKA HNIFPHITLC QFFMCEDSKV DALGEALQTT
160 170 180 190 200
VSRWKCKFSA PLPLELYTSS NFIGLFVKED SAEVLKKFAA DFAAEAASKT
210 220 230 240 250
EVHVEPHKKQ LHVTLAYHFQ ASHLPTLEKL AQNIDVKLGC DWVATIFSRD
260 270 280 290 300
IRFANHETLQ VIYPYTPQND DELELVPGDF IFMSPMEQTS TSEGWIYGTS
310 320 330 340 350
LTTGCSGLLP ENYITKADEC STWIFHGSYS ILNTSSSNSL TFGDGVLERR
360 370 380 390 400
PYEDQGLGET TPLTIICQPM QPLRVNSQPG PQKRCLFVCR HGERMDVVFG
410 420 430 440 450
KYWLSQCFDA KGRYIRTNLN MPHSLPQRSG GFRDYEKDAP ITVFGCMQAR
460 470 480 490 500
LVGEALLESN TIIDHVYCSP SLRCVQTAHN ILKGLQQENH LKIRVEPGLF
510 520 530 540 550
EWTKWVAGST LPAWIPPSEL AAANLSVDTT YRPHIPISKL VVSESYDTYI
560 570 580 590 600
SRSFQVTKEI ISECKSKGNN ILIVAHASSL EACTCQLQGL SPQNSKDFVQ
610 620 630 640
MVRKIPYLGF CSCEELGETG IWQLTDPPIL PLTHGPTGGF NWRETLLQE
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 465 | H → R in BAD96563 (Ref. 3) Curated | 1 | |
Sequence conflicti | 465 | H → R in BAD96566 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_052676 | 68 | A → T1 PublicationCorresponds to variant dbSNP:rs12790613Ensembl. | 1 | |
Natural variantiVAR_061923 | 569 | N → S. Corresponds to variant dbSNP:rs35343548Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB075839 mRNA Translation: BAB85545.1 Different initiation. AK075203 mRNA Translation: BAC11468.1 AK222843 mRNA Translation: BAD96563.1 AK222846 mRNA Translation: BAD96566.1 BC007541 mRNA Translation: AAH07541.2 AF425252 mRNA Translation: AAL16953.1 Different initiation. |
CCDSi | CCDS31694.1 |
RefSeqi | NP_116262.2, NM_032873.4 |
Genome annotation databases
Ensembli | ENST00000284273; ENSP00000284273; ENSG00000154127 |
GeneIDi | 84959 |
KEGGi | hsa:84959 |
MANE-Selecti | ENST00000284273.6; ENSP00000284273.5; NM_032873.5; NP_116262.2 |
UCSCi | uc001pyi.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB075839 mRNA Translation: BAB85545.1 Different initiation. AK075203 mRNA Translation: BAC11468.1 AK222843 mRNA Translation: BAD96563.1 AK222846 mRNA Translation: BAD96566.1 BC007541 mRNA Translation: AAH07541.2 AF425252 mRNA Translation: AAL16953.1 Different initiation. |
CCDSi | CCDS31694.1 |
RefSeqi | NP_116262.2, NM_032873.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CPW | NMR | - | A | 26-76 | [»] | |
2E5K | NMR | - | A | 248-328 | [»] | |
5VR6 | X-ray | 1.87 | A/B | 384-649 | [»] | |
5W5G | X-ray | 2.48 | A/B/C | 383-644 | [»] | |
BMRBi | Q8TF42 | |||||
SMRi | Q8TF42 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 124390, 144 interactors |
IntActi | Q8TF42, 65 interactors |
MINTi | Q8TF42 |
STRINGi | 9606.ENSP00000284273 |
PTM databases
DEPODi | UBASH3B |
GlyGeni | Q8TF42, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q8TF42 |
PhosphoSitePlusi | Q8TF42 |
Genetic variation databases
BioMutai | UBASH3B |
DMDMi | 110287974 |
Proteomic databases
EPDi | Q8TF42 |
jPOSTi | Q8TF42 |
MassIVEi | Q8TF42 |
MaxQBi | Q8TF42 |
PaxDbi | Q8TF42 |
PeptideAtlasi | Q8TF42 |
PRIDEi | Q8TF42 |
ProteomicsDBi | 74552 |
Protocols and materials databases
Antibodypediai | 32801, 144 antibodies from 26 providers |
DNASUi | 84959 |
Genome annotation databases
Ensembli | ENST00000284273; ENSP00000284273; ENSG00000154127 |
GeneIDi | 84959 |
KEGGi | hsa:84959 |
MANE-Selecti | ENST00000284273.6; ENSP00000284273.5; NM_032873.5; NP_116262.2 |
UCSCi | uc001pyi.5, human |
Organism-specific databases
CTDi | 84959 |
DisGeNETi | 84959 |
GeneCardsi | UBASH3B |
HGNCi | HGNC:29884, UBASH3B |
HPAi | ENSG00000154127, Tissue enhanced (brain, lymphoid tissue, placenta) |
MIMi | 609201, gene |
neXtProti | NX_Q8TF42 |
OpenTargetsi | ENSG00000154127 |
PharmGKBi | PA162407840 |
VEuPathDBi | HostDB:ENSG00000154127 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3734, Eukaryota |
GeneTreei | ENSGT00940000156097 |
HOGENOMi | CLU_016516_1_0_1 |
InParanoidi | Q8TF42 |
OMAi | DECGTWV |
OrthoDBi | 243749at2759 |
PhylomeDBi | Q8TF42 |
TreeFami | TF313334 |
Enzyme and pathway databases
PathwayCommonsi | Q8TF42 |
SignaLinki | Q8TF42 |
SIGNORi | Q8TF42 |
Miscellaneous databases
BioGRID-ORCSi | 84959, 11 hits in 1047 CRISPR screens |
ChiTaRSi | UBASH3B, human |
EvolutionaryTracei | Q8TF42 |
GeneWikii | STS-1_(gene) |
GenomeRNAii | 84959 |
Pharosi | Q8TF42, Tbio |
PROi | PR:Q8TF42 |
RNActi | Q8TF42, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000154127, Expressed in cerebellum and 174 other tissues |
Genevisiblei | Q8TF42, HS |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit cd11936, SH3_UBASH3B, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR015940, UBA IPR009060, UBA-like_sf IPR035632, UBASH3B_SH3 |
Pfami | View protein in Pfam PF00300, His_Phos_1, 1 hit PF14604, SH3_9, 1 hit PF00627, UBA, 1 hit |
SMARTi | View protein in SMART SM00326, SH3, 1 hit SM00165, UBA, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit SSF50044, SSF50044, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS50002, SH3, 1 hit PS50030, UBA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBS3B_HUMAN | |
Accessioni | Q8TF42Primary (citable) accession number: Q8TF42 Secondary accession number(s): Q53GT5 Q96NZ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2006 |
Last sequence update: | July 11, 2006 | |
Last modified: | February 23, 2022 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references