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Protein

E3 ubiquitin-protein ligase SH3RF3

Gene

SH3RF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has E3 ubiquitin-protein ligase activity.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri57 – 98RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SH3RF3 (EC:2.3.2.271 Publication)
Alternative name(s):
Plenty of SH3s 2
SH3 domain-containing RING finger protein 3
SH3 multiple domains protein 4
Gene namesi
Name:SH3RF3
Synonyms:POSH21 Publication, SH3MD4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000172985.10
HGNCiHGNC:24699 SH3RF3
neXtProtiNX_Q8TEJ3

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73C → A: Significant reduction in autoubiquitination; when associated with A-75. 1 Publication1
Mutagenesisi75H → A: Significant reduction in autoubiquitination; when associated with A-73. 1 Publication1
Mutagenesisi403I → N: Significant loss of interaction with RAC1; alone or when associated with P-404 and A-406. 1 Publication1
Mutagenesisi404S → P: Reduced JNK activation. Significant loss of interaction with RAC1; alone or when associated with N-403 and A-406. 1 Publication1
Mutagenesisi406P → A: Significant loss of interaction with RAC1; when associated with N-403 and P-404. 1 Publication1

Organism-specific databases

DisGeNETi344558
OpenTargetsiENSG00000172985
PharmGKBiPA162403291

Polymorphism and mutation databases

BioMutaiSH3RF3
DMDMi146325718

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002848831 – 882E3 ubiquitin-protein ligase SH3RF3Add BLAST882

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei400PhosphoserineCombined sources1
Modified residuei797PhosphoserineCombined sources1

Post-translational modificationi

Autoubiquitinated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8TEJ3
MaxQBiQ8TEJ3
PaxDbiQ8TEJ3
PeptideAtlasiQ8TEJ3
PRIDEiQ8TEJ3
ProteomicsDBi74467

PTM databases

iPTMnetiQ8TEJ3
PhosphoSitePlusiQ8TEJ3

Expressioni

Gene expression databases

BgeeiENSG00000172985 Expressed in 179 organ(s), highest expression level in brain
CleanExiHS_SH3RF3
GenevisibleiQ8TEJ3 HS

Organism-specific databases

HPAiHPA044822
HPA048444

Interactioni

Subunit structurei

Interacts (via SH3 domain 3) with PAK2 (PubMed:16374509). Interacts with RAC1 (GTP-bound form) (PubMed:20696164).2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi131307, 39 interactors
DIPiDIP-43763N
IntActiQ8TEJ3, 7 interactors
MINTiQ8TEJ3
STRINGi9606.ENSP00000309186

Structurei

3D structure databases

ProteinModelPortaliQ8TEJ3
SMRiQ8TEJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini194 – 253SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini256 – 319SH3 2PROSITE-ProRule annotationAdd BLAST64
Domaini464 – 525SH3 3PROSITE-ProRule annotationAdd BLAST62
Domaini823 – 882SH3 4PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni369 – 439Interaction with RAC11 PublicationAdd BLAST71

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 34Poly-Arg5
Compositional biasi151 – 155Poly-Gly5

Domaini

The RING finger domain is required for ubiquitin ligase activity and autoubiquitination.1 Publication

Sequence similaritiesi

Belongs to the SH3RF family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri57 – 98RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG410XS5R LUCA
GeneTreeiENSGT00760000119190
HOGENOMiHOG000045564
HOVERGENiHBG069552
InParanoidiQ8TEJ3
KOiK12171
OMAiTTMHPGS
OrthoDBiEOG091G09B6
PhylomeDBiQ8TEJ3
TreeFamiTF105571

Family and domain databases

CDDicd11925 SH3_SH3RF3_3, 1 hit
cd11785 SH3_SH3RF_C, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035816 SH3RF1/SH3RF3_SH3_4
IPR035612 SH3RF3_SH3_3
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00018 SH3_1, 2 hits
PF14604 SH3_9, 2 hits
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00326 SH3, 4 hits
SUPFAMiSSF50044 SSF50044, 4 hits
PROSITEiView protein in PROSITE
PS50002 SH3, 4 hits
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8TEJ3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLGASWLCA SKAAAAAAQS EGDEDRPGER RRRRAAATAA GAGEDMDESS
60 70 80 90 100
LLDLLECSVC LERLDTTAKV LPCQHTFCRR CLESIVCSRH ELRCPECRIL
110 120 130 140 150
VGCGVDELPA NILLVRLLDG IRQRPRAGTS PGGSPPARPI PGQSAAPTLA
160 170 180 190 200
GGGGGAAGST PGSPVFLSAA AGSTAGSLRE LATSRTAPAA KNPCLLPYGK
210 220 230 240 250
ALYSYEGKEP GDLKFNKGDI IVLRRKVDEQ WYHGELHGTQ GFLPASYIQC
260 270 280 290 300
IQPLPHAPPQ GKALYDFEMK DKDQDKDCLT FTKDEILTVL RRVDENWAEG
310 320 330 340 350
MLGDKIGIFP LLYVELNDSA KQLIEMDKPC PAAASSCNAS LPSDSGAVAS
360 370 380 390 400
VAPSPTLSSS GAVSAFQRRV DGKKNTKKRH SFTALSVTHR SSQAASHRHS
410 420 430 440 450
MEISAPVLIS SSDPRAAARI GDLAHLSCAA PTQDVSSSAG STPTAVPRAA
460 470 480 490 500
SVSGEQGTPP KVQLPLNVYL ALYAYKPQKS DELELHKGEM YRVLEKCQDG
510 520 530 540 550
WFKGASLRTG VSGVFPGNYV TPVSRVPAGG AGPPRNNVVG GSPLAKGITT
560 570 580 590 600
TMHPGSGSLS SLATATRPAL PITTPQAHAQ HPTASPPTGS CLRHSAQPTA
610 620 630 640 650
SQARSTISTA AHSAAQAQDR PTATVSPLRT QNSPSRLPAT SLRPHSVVSP
660 670 680 690 700
QHSHQPPVQM CPRPAIPLTS AASAITPPNV SAANLNGEAG GGPIGVLSTS
710 720 730 740 750
SPTNTGCKLD EKKSEKKEKK SGLLKLLAGA STKKKSRSPP SVSPTHDPQV
760 770 780 790 800
AVDALLQGAV GPEVSSLSIH GRAGSCPIES EMQGAMGMEP LHRKAGSLDL
810 820 830 840 850
NFTSPSRQAP LSMAAIRPEP KLLPRERYRV VVSYPPQSEA EIELKEGDIV
860 870 880
FVHKKREDGW YKGTLQRNGR TGLFPGSFVE SF
Length:882
Mass (Da):92,776
Last modified:May 1, 2007 - v2
Checksum:i07DF961AF2C80A08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010906 Genomic DNA No translation available.
AC140485 Genomic DNA No translation available.
AC139850 Genomic DNA No translation available.
AC109344 Genomic DNA No translation available.
DQ307286 mRNA Translation: ABC25188.1
AK074131 mRNA Translation: BAB84957.1
AL831825 mRNA Translation: CAD38539.1
CCDSiCCDS74557.1
RefSeqiNP_001092759.1, NM_001099289.2
UniGeneiHs.171244
Hs.535157
Hs.712087

Genome annotation databases

EnsembliENST00000309415; ENSP00000309186; ENSG00000172985
GeneIDi344558
KEGGihsa:344558
UCSCiuc010ywt.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010906 Genomic DNA No translation available.
AC140485 Genomic DNA No translation available.
AC139850 Genomic DNA No translation available.
AC109344 Genomic DNA No translation available.
DQ307286 mRNA Translation: ABC25188.1
AK074131 mRNA Translation: BAB84957.1
AL831825 mRNA Translation: CAD38539.1
CCDSiCCDS74557.1
RefSeqiNP_001092759.1, NM_001099289.2
UniGeneiHs.171244
Hs.535157
Hs.712087

3D structure databases

ProteinModelPortaliQ8TEJ3
SMRiQ8TEJ3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi131307, 39 interactors
DIPiDIP-43763N
IntActiQ8TEJ3, 7 interactors
MINTiQ8TEJ3
STRINGi9606.ENSP00000309186

PTM databases

iPTMnetiQ8TEJ3
PhosphoSitePlusiQ8TEJ3

Polymorphism and mutation databases

BioMutaiSH3RF3
DMDMi146325718

Proteomic databases

EPDiQ8TEJ3
MaxQBiQ8TEJ3
PaxDbiQ8TEJ3
PeptideAtlasiQ8TEJ3
PRIDEiQ8TEJ3
ProteomicsDBi74467

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000309415; ENSP00000309186; ENSG00000172985
GeneIDi344558
KEGGihsa:344558
UCSCiuc010ywt.2 human

Organism-specific databases

CTDi344558
DisGeNETi344558
EuPathDBiHostDB:ENSG00000172985.10
GeneCardsiSH3RF3
H-InvDBiHIX0023401
HGNCiHGNC:24699 SH3RF3
HPAiHPA044822
HPA048444
neXtProtiNX_Q8TEJ3
OpenTargetsiENSG00000172985
PharmGKBiPA162403291
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG410XS5R LUCA
GeneTreeiENSGT00760000119190
HOGENOMiHOG000045564
HOVERGENiHBG069552
InParanoidiQ8TEJ3
KOiK12171
OMAiTTMHPGS
OrthoDBiEOG091G09B6
PhylomeDBiQ8TEJ3
TreeFamiTF105571

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

ChiTaRSiSH3RF3 human
GenomeRNAii344558
PROiPR:Q8TEJ3

Gene expression databases

BgeeiENSG00000172985 Expressed in 179 organ(s), highest expression level in brain
CleanExiHS_SH3RF3
GenevisibleiQ8TEJ3 HS

Family and domain databases

CDDicd11925 SH3_SH3RF3_3, 1 hit
cd11785 SH3_SH3RF_C, 1 hit
Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035816 SH3RF1/SH3RF3_SH3_4
IPR035612 SH3RF3_SH3_3
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00018 SH3_1, 2 hits
PF14604 SH3_9, 2 hits
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00326 SH3, 4 hits
SUPFAMiSSF50044 SSF50044, 4 hits
PROSITEiView protein in PROSITE
PS50002 SH3, 4 hits
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSH3R3_HUMAN
AccessioniPrimary (citable) accession number: Q8TEJ3
Secondary accession number(s): A0SDZ7, A8MPR1, Q8NDU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 7, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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