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UniProtKB - Q8TDZ2 (MICA1_HUMAN)

Entry version 181 (12 Aug 2020)
Sequence version 2 (15 Mar 2004)
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Protein

[F-actin]-monooxygenase MICAL1

Gene

MICAL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (By similarity). In the absence of actin, it also functions as a NADPH oxidase producing H2O2 (PubMed:21864500, PubMed:26845023). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels (By similarity). May act as Rab effector protein and play a role in vesicle trafficking.By similarityCurated4 Publications

Caution

The reaction mechanism is subject to discussion. Some work suggest MICAL enzymes directly oxidize actin methionine residues to produce methionine-(R)-S-oxide. Other publications suggest that the enzyme functions as a NADPH oxidase producing H2O2 (EC 1.6.3.1) and that it is the produced H2O2 that is responsible for the methionine-(R)-S-oxide production.By similarity2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

FAD1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.28 sec(-1) for NADPH for the NADPH oxidase reaction.1 Publication
  1. KM=4.7 µM for F-actin (at saturing NADPH concentration)1 Publication
  2. KM=375 µM for NADPH (for the NADPH oxidase reaction)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei95FADBy similarity1
    Binding sitei181FAD; via amide nitrogen and carbonyl oxygenBy similarity1
    Binding sitei293FADBy similarity1
    Binding sitei393FADBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi697Zinc 1Combined sources1
    Metal bindingi700Zinc 1Combined sources1
    Metal bindingi718Zinc 1; via pros nitrogenCombined sources1
    Metal bindingi721Zinc 1Combined sources1
    Metal bindingi724Zinc 2Combined sources1
    Metal bindingi727Zinc 2Combined sources1
    Metal bindingi747Zinc 2Combined sources1
    Metal bindingi750Zinc 2; via pros nitrogenCombined sources1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi114 – 116FADBy similarity3
    Nucleotide bindingi121 – 123FADBy similarity3

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    Complete GO annotation on QuickGO ...

    GO - Biological processi

    Complete GO annotation on QuickGO ...

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionActin-binding, Monooxygenase, Oxidoreductase
    LigandFAD, Flavoprotein, Metal-binding, NADP, Zinc

    Enzyme and pathway databases

    Pathway Commons web resource for biological pathway data

    More...    PathwayCommonsi    		Q8TDZ2

    Reactome - a knowledgebase of biological pathways and processes

    More...    Reactomei    		R-HSA-983231, Factors involved in megakaryocyte development and platelet production

    SignaLink: a signaling pathway resource with multi-layered regulatory networks

    More...    SignaLinki    		Q8TDZ2

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    [F-actin]-monooxygenase MICAL1Curated (EC:1.14.13.225By similarity)
    Alternative name(s):
    Molecule interacting with CasL protein 1
    Short name:
    MICAL-1
    NEDD9-interacting protein with calponin homology and LIM domains
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:MICAL1
    Synonyms:MICAL, NICAL
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 6

    Organism-specific databases

    Eukaryotic Pathogen Database Resources

    More...    EuPathDBi    		HostDB:ENSG00000135596.17

    Human Gene Nomenclature Database

    More...    HGNCi    		HGNC:20619, MICAL1

    Online Mendelian Inheritance in Man (OMIM)

    More...    MIMi    		607129, gene

    neXtProt; the human protein knowledge platform

    More...    neXtProti    		NX_Q8TDZ2

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Organism-specific databases

    DisGeNET

    More...    DisGeNETi    		64780

    MalaCards human disease database

    More...    MalaCardsi    		MICAL1

    Open Targets

    More...    OpenTargetsi    		ENSG00000135596

    The Pharmacogenetics and Pharmacogenomics Knowledge Base

    More...    PharmGKBi    		PA134900249

    Miscellaneous databases

    Pharos NIH Druggable Genome Knowledgebase

    More...    Pharosi    		Q8TDZ2, Tbio

    Polymorphism and mutation databases

    BioMuta curated single-nucleotide variation and disease association database

    More...    BioMutai    		MICAL1

    Domain mapping of disease mutations (DMDM)

    More...    DMDMi    		45593495

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000758421 – 1067[F-actin]-monooxygenase MICAL1Add BLAST1067

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei475PhosphothreonineBy similarity1
    Modified residuei617PhosphoserineCombined sources1
    Modified residuei872PhosphoserineCombined sources1
    Modified residuei875PhosphoserineCombined sources1
    Modified residuei876PhosphoserineCombined sources1
    Modified residuei1057PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    Encyclopedia of Proteome Dynamics

    More...    EPDi    		Q8TDZ2

    jPOST - Japan Proteome Standard Repository/Database

    More...    jPOSTi    		Q8TDZ2

    MassIVE - Mass Spectrometry Interactive Virtual Environment

    More...    MassIVEi    		Q8TDZ2

    MaxQB - The MaxQuant DataBase

    More...    MaxQBi    		Q8TDZ2

    PaxDb, a database of protein abundance averages across all three domains of life

    More...    PaxDbi    		Q8TDZ2

    PeptideAtlas

    More...    PeptideAtlasi    		Q8TDZ2

    PRoteomics IDEntifications database

    More...    PRIDEi    		Q8TDZ2

    ProteomicsDB: a multi-organism proteome resource

    More...    ProteomicsDBi    		74376 [Q8TDZ2-1]
    74377 [Q8TDZ2-2]
    74378 [Q8TDZ2-3]
    74379 [Q8TDZ2-4]

    PTM databases

    iPTMnet integrated resource for PTMs in systems biology context

    More...    iPTMneti    		Q8TDZ2

    MetOSite database of methionine sulfoxide sites

    More...    MetOSitei    		Q8TDZ2

    Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

    More...    PhosphoSitePlusi    		Q8TDZ2

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

    Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.1 Publication

    Gene expression databases

    Bgee dataBase for Gene Expression Evolution

    More...    Bgeei    		ENSG00000135596, Expressed in blood and 202 other tissues

    ExpressionAtlas, Differential and Baseline Expression

    More...    ExpressionAtlasi    		Q8TDZ2, baseline and differential

    Genevisible search portal to normalized and curated expression data from Genevestigator

    More...    Genevisiblei    		Q8TDZ2, HS

    Organism-specific databases

    Human Protein Atlas

    More...    HPAi    		ENSG00000135596, Low tissue specificity

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Interacts with STK38 and STK38L (By similarity).

    Interacts with RAB1B, RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); binding to RAB1B is of low affinity compared to other Rab proteins; at least in case of RAB8A and RAB10 can bind 2 molecules of the Rab proteins simultaneously; ternary complex formation of RAB8A, RAB13 and MICAL1 is possible. Associates with the SH3 domain of NEDD9.

    Interacts with VIM and PLXNA3.

    Interacts with RAB1B.

    By similarity5 Publications

    <p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

    GO - Molecular functioni

    Complete GO annotation on QuickGO ...

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...    BioGRIDi    		122290, 28 interactors

    CORUM comprehensive resource of mammalian protein complexes

    More...    CORUMi    		Q8TDZ2

    Protein interaction database and analysis system

    More...    IntActi    		Q8TDZ2, 23 interactors

    Molecular INTeraction database

    More...    MINTi    		Q8TDZ2

    STRING: functional protein association networks

    More...    STRINGi    		9606.ENSP00000351664

    Miscellaneous databases

    RNAct, Protein-RNA interaction predictions for model organisms.

    More...    RNActi    		Q8TDZ2, protein

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    11067
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...    SMRi    		Q8TDZ2

    Database of comparative protein structure models

    More...    ModBasei    		Search...

    Protein Data Bank in Europe - Knowledge Base

    More...    PDBe-KBi    		Search...

    Miscellaneous databases

    Relative evolutionary importance of amino acids within a protein sequence

    More...    EvolutionaryTracei    		Q8TDZ2

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini508 – 612Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST105
    Domaini695 – 757LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST63
    Domaini918 – 1067bMERBPROSITE-ProRule annotation2 PublicationsAdd BLAST150

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 489Monooxygenase domainBy similarityAdd BLAST489

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili646 – 666Sequence analysisAdd BLAST21
    Coiled coili919 – 962Sequence analysisAdd BLAST44
    Coiled coili999 – 1027Sequence analysisAdd BLAST29

    <p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    The bivalent Mical/EHBP Rab binding (bMERB) domain, mediates binding to predominantly Rab8, Rab10, Rab10, Rab13 and Rab15 (in their GTP-bound forms).1 Publication
    The C-terminal coiled coil part contains the plexin-interacting region.

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the Mical family.Curated

    Keywords - Domaini

    Coiled coil, LIM domain

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...    eggNOGi    		KOG1700, Eukaryota

    Ensembl GeneTree

    More...    GeneTreei    		ENSGT00940000159117

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...    HOGENOMi    		CLU_000329_0_0_1

    InParanoid: Eukaryotic Ortholog Groups

    More...    InParanoidi    		Q8TDZ2

    KEGG Orthology (KO)

    More...    KOi    		K19947

    Identification of Orthologs from Complete Genome Data

    More...    OMAi    		CMHRAEN

    Database of Orthologous Groups

    More...    OrthoDBi    		430978at2759

    Database for complete collections of gene phylogenies

    More...    PhylomeDBi    		Q8TDZ2

    TreeFam database of animal gene trees

    More...    TreeFami    		TF324129

    Family and domain databases

    Conserved Domains Database

    More...    CDDi    		cd00014, CH, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...    Gene3Di    		1.10.418.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...    InterProi    		View protein in InterPro
    IPR022735, bMERB_dom
    IPR001715, CH-domain
    IPR036872, CH_dom_sf
    IPR002938, FAD-bd
    IPR036188, FAD/NAD-bd_sf
    IPR029937, MICAL1
    IPR001781, Znf_LIM

    The PANTHER Classification System

    More...    PANTHERi    		PTHR24206:SF59, PTHR24206:SF59, 1 hit

    Pfam protein domain database

    More...    Pfami    		View protein in Pfam
    PF00307, CH, 1 hit
    PF12130, DUF3585, 1 hit
    PF01494, FAD_binding_3, 1 hit
    PF00412, LIM, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...    SMARTi    		View protein in SMART
    SM00033, CH, 1 hit
    SM00132, LIM, 1 hit

    Superfamily database of structural and functional annotation

    More...    SUPFAMi    		SSF47576, SSF47576, 1 hit
    SSF51905, SSF51905, 1 hit

    PROSITE; a protein domain and family database

    More...    PROSITEi    		View protein in PROSITE
    PS51848, BMERB, 1 hit
    PS50021, CH, 1 hit
    PS00478, LIM_DOMAIN_1, 1 hit
    PS50023, LIM_DOMAIN_2, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    This entry describes 4 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q8TDZ2-1) [UniParc]FASTAAdd to basket
    Also known as: MICAL-1a

    This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MASPTSTNPA HAHFESFLQA QLCQDVLSSF QELCGALGLE PGGGLPQYHK 
            60         70         80         90        100
    IKDQLNYWSA KSLWTKLDKR AGQPVYQQGR ACTSTKCLVV GAGPCGLRVA 
           110        120        130        140        150
    VELALLGARV VLVEKRTKFS RHNVLHLWPF TIHDLRALGA KKFYGRFCTG 
           160        170        180        190        200
    TLDHISIRQL QLLLLKVALL LGVEIHWGVT FTGLQPPPRK GSGWRAQLQP 
           210        220        230        240        250
    NPPAQLANYE FDVLISAAGG KFVPEGFKVR EMRGKLAIGI TANFVNGRTV 
           260        270        280        290        300
    EETQVPEISG VARIYNQSFF QSLLKATGID LENIVYYKDD THYFVMTAKK 
           310        320        330        340        350
    QCLLRLGVLR QDWPDTNRLL GSANVVPEAL QRFTRAAADF ATHGKLGKLE 
           360        370        380        390        400
    FAQDAHGQPD VSAFDFTSMM RAESSARVQE KHGARLLLGL VGDCLVEPFW 
           410        420        430        440        450
    PLGTGVARGF LAAFDAAWMV KRWAEGAESL EVLAERESLY QLLSQTSPEN 
           460        470        480        490        500
    MHRNVAQYGL DPATRYPNLN LRAVTPNQVR DLYDVLAKEP VQRNNDKTDT 
           510        520        530        540        550
    GMPATGSAGT QEELLRWCQE QTAGYPGVHV SDLSSSWADG LALCALVYRL 
           560        570        580        590        600
    QPGLLEPSEL QGLGALEATA WALKVAENEL GITPVVSAQA VVAGSDPLGL 
           610        620        630        640        650
    IAYLSHFHSA FKSMAHSPGP VSQASPGTSS AVLFLSKLQR TLQRSRAKEN 
           660        670        680        690        700
    AEDAGGKKLR LEMEAETPST EVPPDPEPGV PLTPPSQHQE AGAGDLCALC 
           710        720        730        740        750
    GEHLYVLERL CVNGHFFHRS CFRCHTCEAT LWPGGYEQHP GDGHFYCLQH 
           760        770        780        790        800
    LPQTDHKAEG SDRGPESPEL PTPSENSMPP GLSTPTASQE GAGPVPDPSQ 
           810        820        830        840        850
    PTRRQIRLSS PERQRLSSLN LTPDPEMEPP PKPPRSCSAL ARHALESSFV 
           860        870        880        890        900
    GWGLPVQSPQ ALVAMEKEEK ESPFSSEEEE EDVPLDSDVE QALQTFAKTS 
           910        920        930        940        950
    GTMNNYPTWR RTLLRRAKEE EMKRFCKAQT IQRRLNEIEA ALRELEAEGV 
           960        970        980        990       1000
    KLELALRRQS SSPEQQKKLW VGQLLQLVDK KNSLVAEEAE LMITVQELNL 
          1010       1020       1030       1040       1050
    EEKQWQLDQE LRGYMNREEN LKTAADRQAE DQVLRKLVDL VNQRDALIRF 
          1060 
    QEERRLSELA LGTGAQG
    Length:1,067
    Mass (Da):117,875
    Last modified:March 15, 2004 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i78C1BBF3E3CCD56B
    GO
    Isoform 2 (identifier: Q8TDZ2-2) [UniParc]FASTAAdd to basket
    Also known as: MICAL-1b

    The sequence of this isoform differs from the canonical sequence as follows:
         312-397: Missing.

    Show »
    Length:981
    Mass (Da):108,552
    Checksum:i8EC7E9C12989C1E9
    GO
    Isoform 3 (identifier: Q8TDZ2-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-735: Missing.
         736-768: YEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESP → MPRLTFAPKGWPHPPTSLHPGQVTDQTTWWLFQ

    Show »
    Length:332
    Mass (Da):37,715
    Checksum:i7FF1E955AE2204DB
    GO
    Isoform 4 (identifier: Q8TDZ2-4) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MSCLSHSSLPSCCPPQEASM

    Show »
    Length:1,086
    Mass (Da):119,821
    Checksum:i947094500071E091
    GO

    <p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

    There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    		Gene namesLengthAnnotation
    H0Y6Z4H0Y6Z4_HUMAN
    [F-actin]-monooxygenase MICAL1
    MICAL1
    		252Annotation score:

    Annotation score:2 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
    Q5TED7Q5TED7_HUMAN
    [F-actin]-monooxygenase MICAL1
    MICAL1
    		244Annotation score:

    Annotation score:1 out of 5

    <p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti767S → C in BAB15124 (PubMed:14702039).Curated1
    Sequence conflicti816L → S in BAH12301 (PubMed:14702039).Curated1
    Sequence conflicti877E → V in BAH12301 (PubMed:14702039).Curated1
    Sequence conflicti887S → L in CAB59266 (PubMed:17974005).Curated1
    Sequence conflicti981K → N in BAB13949 (PubMed:14702039).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_06706312A → S. Corresponds to variant dbSNP:rs4946977Ensembl.1
    Natural variantiVAR_05015312A → T. Corresponds to variant dbSNP:rs4946977Ensembl.1
    Natural variantiVAR_050154153D → A. Corresponds to variant dbSNP:rs34726911Ensembl.1
    Natural variantiVAR_067064195R → H. Corresponds to variant dbSNP:rs34699467Ensembl.1
    Natural variantiVAR_036191309L → M in a breast cancer sample; somatic mutation. 1 Publication1
    Natural variantiVAR_067065453R → C1 PublicationCorresponds to variant dbSNP:rs17854785Ensembl.1
    Natural variantiVAR_067066624A → T1 PublicationCorresponds to variant dbSNP:rs17850590Ensembl.1
    Natural variantiVAR_017903758A → E. Corresponds to variant dbSNP:rs9320288Ensembl.1
    Natural variantiVAR_067067758A → K Requires 2 nucleotide substitutions. 3 PublicationsCorresponds to variant dbSNP:rs35260632Ensembl.1
    Natural variantiVAR_067068758A → S. Corresponds to variant dbSNP:rs59056467Ensembl.1
    Natural variantiVAR_061355758A → T. Corresponds to variant dbSNP:rs59056467Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0096371 – 735Missing in isoform 3. 1 PublicationAdd BLAST735
    Alternative sequenceiVSP_0425901M → MSCLSHSSLPSCCPPQEASM in isoform 4. 1 Publication1
    Alternative sequenceiVSP_009639312 – 397Missing in isoform 2. 1 PublicationAdd BLAST86
    Alternative sequenceiVSP_009638736 – 768YEQHP…GPESP → MPRLTFAPKGWPHPPTSLHP GQVTDQTTWWLFQ in isoform 3. 1 PublicationAdd BLAST33

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...    EMBLi

    GenBank nucleotide sequence database

    More...    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...    DDBJi
    Links Updated
    		AB048948 mRNA Translation: BAB86289.1
    AK024500 mRNA Translation: BAB15790.1
    AK021999 mRNA Translation: BAB13949.1
    AK025392 mRNA Translation: BAB15124.1
    AK296284 mRNA Translation: BAH12301.1
    AL109947 Genomic DNA No translation available.
    CH471051 Genomic DNA Translation: EAW48344.1
    CH471051 Genomic DNA Translation: EAW48345.1
    BC009972 mRNA Translation: AAH09972.2
    BC042144 mRNA Translation: AAH42144.1
    BC052983 mRNA Translation: AAH52983.1
    AL122098 mRNA Translation: CAB59266.1

    The Consensus CDS (CCDS) project

    More...    CCDSi    		CCDS5076.1 [Q8TDZ2-1]
    CCDS55047.1 [Q8TDZ2-2]
    CCDS69170.1 [Q8TDZ2-4]

    Protein sequence database of the Protein Information Resource

    More...    PIRi    		T34532

    NCBI Reference Sequences

    More...    RefSeqi    		NP_001152763.1, NM_001159291.1 [Q8TDZ2-2]
    NP_001273542.1, NM_001286613.1 [Q8TDZ2-4]
    NP_073602.3, NM_022765.3 [Q8TDZ2-1]

    Genome annotation databases

    Ensembl eukaryotic genome annotation project

    More...    Ensembli    		ENST00000358577; ENSP00000351385; ENSG00000135596 [Q8TDZ2-2]
    ENST00000358807; ENSP00000351664; ENSG00000135596 [Q8TDZ2-1]
    ENST00000630715; ENSP00000486901; ENSG00000135596 [Q8TDZ2-4]

    Database of genes from NCBI RefSeq genomes

    More...    GeneIDi    		64780

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...    KEGGi    		hsa:64780

    UCSC genome browser

    More...    UCSCi    		uc003ptk.4, human [Q8TDZ2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		AB048948 mRNA Translation: BAB86289.1
    AK024500 mRNA Translation: BAB15790.1
    AK021999 mRNA Translation: BAB13949.1
    AK025392 mRNA Translation: BAB15124.1
    AK296284 mRNA Translation: BAH12301.1
    AL109947 Genomic DNA No translation available.
    CH471051 Genomic DNA Translation: EAW48344.1
    CH471051 Genomic DNA Translation: EAW48345.1
    BC009972 mRNA Translation: AAH09972.2
    BC042144 mRNA Translation: AAH42144.1
    BC052983 mRNA Translation: AAH52983.1
    AL122098 mRNA Translation: CAB59266.1
    CCDSiCCDS5076.1 [Q8TDZ2-1]
    CCDS55047.1 [Q8TDZ2-2]
    CCDS69170.1 [Q8TDZ2-4]
    PIRiT34532
    RefSeqiNP_001152763.1, NM_001159291.1 [Q8TDZ2-2]
    NP_001273542.1, NM_001286613.1 [Q8TDZ2-4]
    NP_073602.3, NM_022765.3 [Q8TDZ2-1]

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...    PDBei

    Protein Data Bank RCSB

    More...    RCSB PDBi

    Protein Data Bank Japan

    More...    PDBji
    Links Updated
    		PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1WYLNMR-A510-612[»]
    2CO8NMR-A687-755[»]
    2DK9NMR-A506-614[»]
    5LE0X-ray3.30B918-1067[»]
    5LPNX-ray2.80B918-1067[»]
    SMRiQ8TDZ2
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein-protein interaction databases

    BioGRIDi122290, 28 interactors
    CORUMiQ8TDZ2
    IntActiQ8TDZ2, 23 interactors
    MINTiQ8TDZ2
    STRINGi9606.ENSP00000351664

    PTM databases

    iPTMnetiQ8TDZ2
    MetOSiteiQ8TDZ2
    PhosphoSitePlusiQ8TDZ2

    Polymorphism and mutation databases

    BioMutaiMICAL1
    DMDMi45593495

    Proteomic databases

    EPDiQ8TDZ2
    jPOSTiQ8TDZ2
    MassIVEiQ8TDZ2
    MaxQBiQ8TDZ2
    PaxDbiQ8TDZ2
    PeptideAtlasiQ8TDZ2
    PRIDEiQ8TDZ2
    ProteomicsDBi74376 [Q8TDZ2-1]
    74377 [Q8TDZ2-2]
    74378 [Q8TDZ2-3]
    74379 [Q8TDZ2-4]

    Protocols and materials databases

    Antibodypedia a portal for validated antibodies

    More...    Antibodypediai    		32258, 256 antibodies

    Genome annotation databases

    EnsembliENST00000358577; ENSP00000351385; ENSG00000135596 [Q8TDZ2-2]
    ENST00000358807; ENSP00000351664; ENSG00000135596 [Q8TDZ2-1]
    ENST00000630715; ENSP00000486901; ENSG00000135596 [Q8TDZ2-4]
    GeneIDi64780
    KEGGihsa:64780
    UCSCiuc003ptk.4, human [Q8TDZ2-1]

    Organism-specific databases

    Comparative Toxicogenomics Database

    More...    CTDi    		64780
    DisGeNETi64780
    EuPathDBiHostDB:ENSG00000135596.17

    GeneCards: human genes, protein and diseases

    More...    GeneCardsi    		MICAL1
    HGNCiHGNC:20619, MICAL1
    HPAiENSG00000135596, Low tissue specificity
    MalaCardsiMICAL1
    MIMi607129, gene
    neXtProtiNX_Q8TDZ2
    OpenTargetsiENSG00000135596
    PharmGKBiPA134900249

    GenAtlas: human gene database

    More...    GenAtlasi    		Search...

    Phylogenomic databases

    eggNOGiKOG1700, Eukaryota
    GeneTreeiENSGT00940000159117
    HOGENOMiCLU_000329_0_0_1
    InParanoidiQ8TDZ2
    KOiK19947
    OMAiCMHRAEN
    OrthoDBi430978at2759
    PhylomeDBiQ8TDZ2
    TreeFamiTF324129

    Enzyme and pathway databases

    PathwayCommonsiQ8TDZ2
    ReactomeiR-HSA-983231, Factors involved in megakaryocyte development and platelet production
    SignaLinkiQ8TDZ2

    Miscellaneous databases

    BioGRID ORCS database of CRISPR phenotype screens

    More...    BioGRID-ORCSi    		64780, 3 hits in 871 CRISPR screens

    ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

    More...    ChiTaRSi    		MICAL1, human
    EvolutionaryTraceiQ8TDZ2

    The Gene Wiki collection of pages on human genes and proteins

    More...    GeneWikii    		MICAL1

    Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

    More...    GenomeRNAii    		64780
    PharosiQ8TDZ2, Tbio

    Protein Ontology

    More...    PROi    		PR:Q8TDZ2
    RNActiQ8TDZ2, protein

    The Stanford Online Universal Resource for Clones and ESTs

    More...    SOURCEi    		Search...

    Gene expression databases

    BgeeiENSG00000135596, Expressed in blood and 202 other tissues
    ExpressionAtlasiQ8TDZ2, baseline and differential
    GenevisibleiQ8TDZ2, HS

    Family and domain databases

    CDDicd00014, CH, 1 hit
    Gene3Di1.10.418.10, 1 hit
    InterProiView protein in InterPro
    IPR022735, bMERB_dom
    IPR001715, CH-domain
    IPR036872, CH_dom_sf
    IPR002938, FAD-bd
    IPR036188, FAD/NAD-bd_sf
    IPR029937, MICAL1
    IPR001781, Znf_LIM
    PANTHERiPTHR24206:SF59, PTHR24206:SF59, 1 hit
    PfamiView protein in Pfam
    PF00307, CH, 1 hit
    PF12130, DUF3585, 1 hit
    PF01494, FAD_binding_3, 1 hit
    PF00412, LIM, 1 hit
    SMARTiView protein in SMART
    SM00033, CH, 1 hit
    SM00132, LIM, 1 hit
    SUPFAMiSSF47576, SSF47576, 1 hit
    SSF51905, SSF51905, 1 hit
    PROSITEiView protein in PROSITE
    PS51848, BMERB, 1 hit
    PS50021, CH, 1 hit
    PS00478, LIM_DOMAIN_1, 1 hit
    PS50023, LIM_DOMAIN_2, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...    ProtoNeti    		Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...    MobiDBi    		Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMICA1_HUMAN
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8TDZ2
    Secondary accession number(s): B7Z3R5
    , E1P5F0, Q7Z633, Q8IVS9, Q96G47, Q9H6X6, Q9H7I0, Q9HAA1, Q9UFF7
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: March 15, 2004
    Last modified: August 12, 2020
    This is version 181 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families
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