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Entry version 189 (25 May 2022)
Sequence version 2 (15 Mar 2004)
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Protein

[F-actin]-monooxygenase MICAL1

Gene

MICAL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed:29343822).

In the absence of actin, it also functions as a NADPH oxidase producing H2O2 (PubMed:21864500, PubMed:26845023, PubMed:29343822).

Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels (By similarity).

May act as Rab effector protein and play a role in vesicle trafficking.

By similarity1 Publication5 Publications

Caution

The reaction mechanism is subject to discussion. Some work suggest MICAL enzymes directly oxidize actin methionine residues to produce methionine-(R)-S-oxide. Other publications suggest that the enzyme functions as a NADPH oxidase producing H2O2 (EC 1.6.3.1) and that it is the produced H2O2 that is responsible for the methionine-(R)-S-oxide production.By similarity2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

FAD1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.28 sec(-1) for NADPH for the NADPH oxidase reaction.1 Publication
  1. KM=4.7 µM for F-actin (at saturating NADPH concentration)1 Publication
  2. KM=375 µM for NADPH (for the NADPH oxidase reaction)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei95FADBy similarity1
Binding sitei181FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei293FADBy similarity1
Binding sitei393FADBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi697Zinc 1Combined sources1
Metal bindingi700Zinc 1Combined sources1
Metal bindingi718Zinc 1; via pros nitrogenCombined sources1
Metal bindingi721Zinc 1Combined sources1
Metal bindingi724Zinc 2Combined sources1
Metal bindingi727Zinc 2Combined sources1
Metal bindingi747Zinc 2Combined sources1
Metal bindingi750Zinc 2; via pros nitrogenCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi114 – 116FADBy similarity3
Nucleotide bindingi121 – 123FADBy similarity3

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActin-binding, Monooxygenase, Oxidoreductase
LigandFAD, Flavoprotein, Metal-binding, NADP, Zinc

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
1.14.13.225, 2681

Pathway Commons web resource for biological pathway data

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PathwayCommonsi
Q8TDZ2

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-HSA-983231, Factors involved in megakaryocyte development and platelet production

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
Q8TDZ2

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...
SignaLinki
Q8TDZ2

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
[F-actin]-monooxygenase MICAL1Curated (EC:1.14.13.2251 Publication, EC:1.6.3.13 Publications)
Alternative name(s):
Molecule interacting with CasL protein 1
Short name:
MICAL-1
NEDD9-interacting protein with calponin homology and LIM domains
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:MICAL1
Synonyms:MICAL, NICAL
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 6

Organism-specific databases

Human Gene Nomenclature Database

More...
HGNCi
HGNC:20619, MICAL1

Online Mendelian Inheritance in Man (OMIM)

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MIMi
607129, gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_Q8TDZ2

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
HostDB:ENSG00000135596

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

DisGeNET

More...
DisGeNETi
64780

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

More...
GeneReviewsi
MICAL1

MalaCards human disease database

More...
MalaCardsi
MICAL1

Open Targets

More...
OpenTargetsi
ENSG00000135596

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...
PharmGKBi
PA134900249

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...
Pharosi
Q8TDZ2, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...
BioMutai
MICAL1

Domain mapping of disease mutations (DMDM)

More...
DMDMi
45593495

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000758421 – 1067[F-actin]-monooxygenase MICAL1Add BLAST1067

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei475PhosphothreonineBy similarity1
Modified residuei617PhosphoserineCombined sources1
Modified residuei872PhosphoserineCombined sources1
Modified residuei875PhosphoserineCombined sources1
Modified residuei876PhosphoserineCombined sources1
Modified residuei1057PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
Q8TDZ2

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q8TDZ2

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...
MassIVEi
Q8TDZ2

MaxQB - The MaxQuant DataBase

More...
MaxQBi
Q8TDZ2

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q8TDZ2

PeptideAtlas

More...
PeptideAtlasi
Q8TDZ2

PRoteomics IDEntifications database

More...
PRIDEi
Q8TDZ2

ProteomicsDB: a multi-organism proteome resource

More...
ProteomicsDBi
74376 [Q8TDZ2-1]
74377 [Q8TDZ2-2]
74378 [Q8TDZ2-3]
74379 [Q8TDZ2-4]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...
GlyGeni
Q8TDZ2, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q8TDZ2

MetOSite database of methionine sulfoxide sites

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MetOSitei
Q8TDZ2

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q8TDZ2

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSG00000135596, Expressed in blood and 203 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q8TDZ2, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q8TDZ2, HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
ENSG00000135596, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with STK38 and STK38L (By similarity).

Interacts with RAB1B, RAB8A, RAB10, RAB13 and RAB15 (in their GTP-bound forms); binding to RAB1B is of low affinity compared to other Rab proteins; at least in case of RAB8A and RAB10 can bind 2 molecules of the Rab proteins simultaneously; ternary complex formation of RAB8A, RAB13 and MICAL1 is possible. Associates with the SH3 domain of NEDD9.

Interacts with VIM and PLXNA3.

Interacts with RAB1B.

By similarity5 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
122290, 73 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q8TDZ2

Protein interaction database and analysis system

More...
IntActi
Q8TDZ2, 24 interactors

Molecular INTeraction database

More...
MINTi
Q8TDZ2

STRING: functional protein association networks

More...
STRINGi
9606.ENSP00000351664

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q8TDZ2, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11067
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...
AlphaFoldDBi
Q8TDZ2

Biological Magnetic Resonance Data Bank

More...
BMRBi
Q8TDZ2

Small Angle Scattering Biological Data Bank

More...
SASBDBi
Q8TDZ2

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q8TDZ2

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q8TDZ2

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini508 – 612Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST105
Domaini695 – 757LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST63
Domaini918 – 1067bMERBPROSITE-ProRule annotation2 PublicationsAdd BLAST150

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 489Monooxygenase domainBy similarityAdd BLAST489
Regioni645 – 688DisorderedSequence analysisAdd BLAST44
Regioni755 – 838DisorderedSequence analysisAdd BLAST84
Regioni867 – 886DisorderedSequence analysisAdd BLAST20

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili646 – 666Sequence analysisAdd BLAST21
Coiled coili919 – 962Sequence analysisAdd BLAST44
Coiled coili999 – 1027Sequence analysisAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi645 – 665Basic and acidic residuesSequence analysisAdd BLAST21
Compositional biasi776 – 795Polar residuesSequence analysisAdd BLAST20
Compositional biasi802 – 821Polar residuesSequence analysisAdd BLAST20

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The bivalent Mical/EHBP Rab binding (bMERB) domain, mediates binding to predominantly Rab8, Rab10, Rab10, Rab13 and Rab15 (in their GTP-bound forms).1 Publication
The C-terminal coiled coil part contains the plexin-interacting region.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Mical family.Curated

Keywords - Domaini

Coiled coil, LIM domain

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1700, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00940000159117

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_000329_0_0_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q8TDZ2

Identification of Orthologs from Complete Genome Data

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OMAi
CKAQSIQ

Database of Orthologous Groups

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OrthoDBi
430978at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q8TDZ2

TreeFam database of animal gene trees

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TreeFami
TF324129

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00014, CH, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.10.418.10, 1 hit
3.50.50.60, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR022735, bMERB_dom
IPR001715, CH-domain
IPR036872, CH_dom_sf
IPR002938, FAD-bd
IPR036188, FAD/NAD-bd_sf
IPR045268, LIMA-like
IPR029937, MICAL1
IPR001781, Znf_LIM

The PANTHER Classification System

More...
PANTHERi
PTHR24206, PTHR24206, 1 hit
PTHR24206:SF59, PTHR24206:SF59, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00307, CH, 1 hit
PF12130, DUF3585, 1 hit
PF01494, FAD_binding_3, 1 hit
PF00412, LIM, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00033, CH, 1 hit
SM00132, LIM, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47576, SSF47576, 1 hit
SSF51905, SSF51905, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51848, BMERB, 1 hit
PS50021, CH, 1 hit
PS00478, LIM_DOMAIN_1, 1 hit
PS50023, LIM_DOMAIN_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 4 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8TDZ2-1) [UniParc]FASTAAdd to basket
Also known as: MICAL-1a

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASPTSTNPA HAHFESFLQA QLCQDVLSSF QELCGALGLE PGGGLPQYHK
60 70 80 90 100
IKDQLNYWSA KSLWTKLDKR AGQPVYQQGR ACTSTKCLVV GAGPCGLRVA
110 120 130 140 150
VELALLGARV VLVEKRTKFS RHNVLHLWPF TIHDLRALGA KKFYGRFCTG
160 170 180 190 200
TLDHISIRQL QLLLLKVALL LGVEIHWGVT FTGLQPPPRK GSGWRAQLQP
210 220 230 240 250
NPPAQLANYE FDVLISAAGG KFVPEGFKVR EMRGKLAIGI TANFVNGRTV
260 270 280 290 300
EETQVPEISG VARIYNQSFF QSLLKATGID LENIVYYKDD THYFVMTAKK
310 320 330 340 350
QCLLRLGVLR QDWPDTNRLL GSANVVPEAL QRFTRAAADF ATHGKLGKLE
360 370 380 390 400
FAQDAHGQPD VSAFDFTSMM RAESSARVQE KHGARLLLGL VGDCLVEPFW
410 420 430 440 450
PLGTGVARGF LAAFDAAWMV KRWAEGAESL EVLAERESLY QLLSQTSPEN
460 470 480 490 500
MHRNVAQYGL DPATRYPNLN LRAVTPNQVR DLYDVLAKEP VQRNNDKTDT
510 520 530 540 550
GMPATGSAGT QEELLRWCQE QTAGYPGVHV SDLSSSWADG LALCALVYRL
560 570 580 590 600
QPGLLEPSEL QGLGALEATA WALKVAENEL GITPVVSAQA VVAGSDPLGL
610 620 630 640 650
IAYLSHFHSA FKSMAHSPGP VSQASPGTSS AVLFLSKLQR TLQRSRAKEN
660 670 680 690 700
AEDAGGKKLR LEMEAETPST EVPPDPEPGV PLTPPSQHQE AGAGDLCALC
710 720 730 740 750
GEHLYVLERL CVNGHFFHRS CFRCHTCEAT LWPGGYEQHP GDGHFYCLQH
760 770 780 790 800
LPQTDHKAEG SDRGPESPEL PTPSENSMPP GLSTPTASQE GAGPVPDPSQ
810 820 830 840 850
PTRRQIRLSS PERQRLSSLN LTPDPEMEPP PKPPRSCSAL ARHALESSFV
860 870 880 890 900
GWGLPVQSPQ ALVAMEKEEK ESPFSSEEEE EDVPLDSDVE QALQTFAKTS
910 920 930 940 950
GTMNNYPTWR RTLLRRAKEE EMKRFCKAQT IQRRLNEIEA ALRELEAEGV
960 970 980 990 1000
KLELALRRQS SSPEQQKKLW VGQLLQLVDK KNSLVAEEAE LMITVQELNL
1010 1020 1030 1040 1050
EEKQWQLDQE LRGYMNREEN LKTAADRQAE DQVLRKLVDL VNQRDALIRF
1060
QEERRLSELA LGTGAQG
Length:1,067
Mass (Da):117,875
Last modified:March 15, 2004 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i78C1BBF3E3CCD56B
GO
Isoform 2 (identifier: Q8TDZ2-2) [UniParc]FASTAAdd to basket
Also known as: MICAL-1b

The sequence of this isoform differs from the canonical sequence as follows:
     312-397: Missing.

Show »
Length:981
Mass (Da):108,552
Checksum:i8EC7E9C12989C1E9
GO
Isoform 3 (identifier: Q8TDZ2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-735: Missing.
     736-768: YEQHPGDGHFYCLQHLPQTDHKAEGSDRGPESP → MPRLTFAPKGWPHPPTSLHPGQVTDQTTWWLFQ

Show »
Length:332
Mass (Da):37,715
Checksum:i7FF1E955AE2204DB
GO
Isoform 4 (identifier: Q8TDZ2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSCLSHSSLPSCCPPQEASM

Show »
Length:1,086
Mass (Da):119,821
Checksum:i947094500071E091
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y6Z4H0Y6Z4_HUMAN
[F-actin]-monooxygenase MICAL1
MICAL1
252Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
Q5TED7Q5TED7_HUMAN
[F-actin]-monooxygenase MICAL1
MICAL1
244Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti767S → C in BAB15124 (PubMed:14702039).Curated1
Sequence conflicti816L → S in BAH12301 (PubMed:14702039).Curated1
Sequence conflicti877E → V in BAH12301 (PubMed:14702039).Curated1
Sequence conflicti887S → L in CAB59266 (PubMed:17974005).Curated1
Sequence conflicti981K → N in BAB13949 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_06706312A → S. Corresponds to variant dbSNP:rs4946977Ensembl.1
Natural variantiVAR_05015312A → T. Corresponds to variant dbSNP:rs4946977Ensembl.1
Natural variantiVAR_050154153D → A. Corresponds to variant dbSNP:rs34726911Ensembl.1
Natural variantiVAR_067064195R → H. Corresponds to variant dbSNP:rs34699467EnsemblClinVar.1
Natural variantiVAR_036191309L → M in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_067065453R → C1 PublicationCorresponds to variant dbSNP:rs17854785Ensembl.1
Natural variantiVAR_067066624A → T1 PublicationCorresponds to variant dbSNP:rs17850590Ensembl.1
Natural variantiVAR_017903758A → E. Corresponds to variant dbSNP:rs9320288Ensembl.1
Natural variantiVAR_067067758A → K Requires 2 nucleotide substitutions. 3 PublicationsCorresponds to variant dbSNP:rs35260632Ensembl.1
Natural variantiVAR_067068758A → S. Corresponds to variant dbSNP:rs59056467Ensembl.1
Natural variantiVAR_061355758A → T. Corresponds to variant dbSNP:rs59056467Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0096371 – 735Missing in isoform 3. 1 PublicationAdd BLAST735
Alternative sequenceiVSP_0425901M → MSCLSHSSLPSCCPPQEASM in isoform 4. 1 Publication1
Alternative sequenceiVSP_009639312 – 397Missing in isoform 2. 1 PublicationAdd BLAST86
Alternative sequenceiVSP_009638736 – 768YEQHP…GPESP → MPRLTFAPKGWPHPPTSLHP GQVTDQTTWWLFQ in isoform 3. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AB048948 mRNA Translation: BAB86289.1
AK024500 mRNA Translation: BAB15790.1
AK021999 mRNA Translation: BAB13949.1
AK025392 mRNA Translation: BAB15124.1
AK296284 mRNA Translation: BAH12301.1
AL109947 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48344.1
CH471051 Genomic DNA Translation: EAW48345.1
BC009972 mRNA Translation: AAH09972.2
BC042144 mRNA Translation: AAH42144.1
BC052983 mRNA Translation: AAH52983.1
AL122098 mRNA Translation: CAB59266.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS5076.1 [Q8TDZ2-1]
CCDS55047.1 [Q8TDZ2-2]
CCDS69170.1 [Q8TDZ2-4]

Protein sequence database of the Protein Information Resource

More...
PIRi
T34532

NCBI Reference Sequences

More...
RefSeqi
NP_001152763.1, NM_001159291.1 [Q8TDZ2-2]
NP_001273542.1, NM_001286613.1 [Q8TDZ2-4]
NP_073602.3, NM_022765.3 [Q8TDZ2-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000358577.7; ENSP00000351385.3; ENSG00000135596.18 [Q8TDZ2-2]
ENST00000358807.8; ENSP00000351664.3; ENSG00000135596.18
ENST00000630715.2; ENSP00000486901.1; ENSG00000135596.18 [Q8TDZ2-4]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
64780

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:64780

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...
MANE-Selecti
ENST00000358807.8; ENSP00000351664.3; NM_022765.4; NP_073602.3

UCSC genome browser

More...
UCSCi
uc003ptk.4, human [Q8TDZ2-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048948 mRNA Translation: BAB86289.1
AK024500 mRNA Translation: BAB15790.1
AK021999 mRNA Translation: BAB13949.1
AK025392 mRNA Translation: BAB15124.1
AK296284 mRNA Translation: BAH12301.1
AL109947 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48344.1
CH471051 Genomic DNA Translation: EAW48345.1
BC009972 mRNA Translation: AAH09972.2
BC042144 mRNA Translation: AAH42144.1
BC052983 mRNA Translation: AAH52983.1
AL122098 mRNA Translation: CAB59266.1
CCDSiCCDS5076.1 [Q8TDZ2-1]
CCDS55047.1 [Q8TDZ2-2]
CCDS69170.1 [Q8TDZ2-4]
PIRiT34532
RefSeqiNP_001152763.1, NM_001159291.1 [Q8TDZ2-2]
NP_001273542.1, NM_001286613.1 [Q8TDZ2-4]
NP_073602.3, NM_022765.3 [Q8TDZ2-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WYLNMR-A510-612[»]
2CO8NMR-A687-755[»]
2DK9NMR-A506-614[»]
5LE0X-ray3.30B918-1067[»]
5LPNX-ray2.80B918-1067[»]
6KU0X-ray1.60B/D799-822[»]
AlphaFoldDBiQ8TDZ2
BMRBiQ8TDZ2
SASBDBiQ8TDZ2
SMRiQ8TDZ2
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi122290, 73 interactors
CORUMiQ8TDZ2
IntActiQ8TDZ2, 24 interactors
MINTiQ8TDZ2
STRINGi9606.ENSP00000351664

PTM databases

GlyGeniQ8TDZ2, 1 site, 1 O-linked glycan (1 site)
iPTMnetiQ8TDZ2
MetOSiteiQ8TDZ2
PhosphoSitePlusiQ8TDZ2

Genetic variation databases

BioMutaiMICAL1
DMDMi45593495

Proteomic databases

EPDiQ8TDZ2
jPOSTiQ8TDZ2
MassIVEiQ8TDZ2
MaxQBiQ8TDZ2
PaxDbiQ8TDZ2
PeptideAtlasiQ8TDZ2
PRIDEiQ8TDZ2
ProteomicsDBi74376 [Q8TDZ2-1]
74377 [Q8TDZ2-2]
74378 [Q8TDZ2-3]
74379 [Q8TDZ2-4]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
32258, 265 antibodies from 24 providers

The DNASU plasmid repository

More...
DNASUi
64780

Genome annotation databases

EnsembliENST00000358577.7; ENSP00000351385.3; ENSG00000135596.18 [Q8TDZ2-2]
ENST00000358807.8; ENSP00000351664.3; ENSG00000135596.18
ENST00000630715.2; ENSP00000486901.1; ENSG00000135596.18 [Q8TDZ2-4]
GeneIDi64780
KEGGihsa:64780
MANE-SelectiENST00000358807.8; ENSP00000351664.3; NM_022765.4; NP_073602.3
UCSCiuc003ptk.4, human [Q8TDZ2-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
64780
DisGeNETi64780

GeneCards: human genes, protein and diseases

More...
GeneCardsi
MICAL1
GeneReviewsiMICAL1
HGNCiHGNC:20619, MICAL1
HPAiENSG00000135596, Low tissue specificity
MalaCardsiMICAL1
MIMi607129, gene
neXtProtiNX_Q8TDZ2
OpenTargetsiENSG00000135596
PharmGKBiPA134900249
VEuPathDBiHostDB:ENSG00000135596

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1700, Eukaryota
GeneTreeiENSGT00940000159117
HOGENOMiCLU_000329_0_0_1
InParanoidiQ8TDZ2
OMAiCKAQSIQ
OrthoDBi430978at2759
PhylomeDBiQ8TDZ2
TreeFamiTF324129

Enzyme and pathway databases

BRENDAi1.14.13.225, 2681
PathwayCommonsiQ8TDZ2
ReactomeiR-HSA-983231, Factors involved in megakaryocyte development and platelet production
SABIO-RKiQ8TDZ2
SignaLinkiQ8TDZ2

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
64780, 10 hits in 1078 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
MICAL1, human
EvolutionaryTraceiQ8TDZ2

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
MICAL1

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
64780
PharosiQ8TDZ2, Tbio

Protein Ontology

More...
PROi
PR:Q8TDZ2
RNActiQ8TDZ2, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000135596, Expressed in blood and 203 other tissues
ExpressionAtlasiQ8TDZ2, baseline and differential
GenevisibleiQ8TDZ2, HS

Family and domain databases

CDDicd00014, CH, 1 hit
Gene3Di1.10.418.10, 1 hit
3.50.50.60, 1 hit
InterProiView protein in InterPro
IPR022735, bMERB_dom
IPR001715, CH-domain
IPR036872, CH_dom_sf
IPR002938, FAD-bd
IPR036188, FAD/NAD-bd_sf
IPR045268, LIMA-like
IPR029937, MICAL1
IPR001781, Znf_LIM
PANTHERiPTHR24206, PTHR24206, 1 hit
PTHR24206:SF59, PTHR24206:SF59, 1 hit
PfamiView protein in Pfam
PF00307, CH, 1 hit
PF12130, DUF3585, 1 hit
PF01494, FAD_binding_3, 1 hit
PF00412, LIM, 1 hit
SMARTiView protein in SMART
SM00033, CH, 1 hit
SM00132, LIM, 1 hit
SUPFAMiSSF47576, SSF47576, 1 hit
SSF51905, SSF51905, 1 hit
PROSITEiView protein in PROSITE
PS51848, BMERB, 1 hit
PS50021, CH, 1 hit
PS00478, LIM_DOMAIN_1, 1 hit
PS50023, LIM_DOMAIN_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMICA1_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8TDZ2
Secondary accession number(s): B7Z3R5
, E1P5F0, Q7Z633, Q8IVS9, Q96G47, Q9H6X6, Q9H7I0, Q9HAA1, Q9UFF7
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 15, 2004
Last modified: May 25, 2022
This is version 189 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
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