UniProtKB - Q8TDX7 (NEK7_HUMAN)
Serine/threonine-protein kinase Nek7
NEK7
Functioni
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132).
Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596).
Phosphorylates RPS6KB1 (By similarity).
Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).
Essential protein for NLRP3 inflammasome assembly and activation that acts downstream stimuli such as K+ efflux. Dispensable for the induction of core inflammasome components, but necessary for subsequent formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division. Exerts its effects on the NLRP3 inflammasome independently of its kinase activity (PubMed:26642356).
By similarity5 PublicationsCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 63 | ATP | 1 | |
Sitei | 97 | Autoinhibitory | 1 | |
Active sitei | 161 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 40 – 48 | ATP | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cellular response to potassium ion Source: UniProtKB
- positive regulation of NLRP3 inflammasome complex assembly Source: UniProtKB
- positive regulation of telomerase activity Source: BHF-UCL
- positive regulation of telomere capping Source: BHF-UCL
- positive regulation of telomere maintenance via telomerase Source: BHF-UCL
- protein phosphorylation Source: UniProtKB
- regulation of mitotic cell cycle Source: UniProtKB
- spindle assembly Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8TDX7 |
Reactomei | R-HSA-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q8TDX7 |
SIGNORi | Q8TDX7 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase Nek7 (EC:2.7.11.1)Alternative name(s): Never in mitosis A-related kinase 7 Short name: NimA-related protein kinase 7 |
Gene namesi | Name:NEK7 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:13386, NEK7 |
MIMi | 606848, gene |
neXtProti | NX_Q8TDX7 |
VEuPathDBi | HostDB:ENSG00000151414 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- spindle pole 1 Publication
- centrosome 2 Publications
Note: Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity).By similarity1 Publication
Cytoskeleton
- microtubule Source: UniProtKB-KW
- microtubule organizing center Source: UniProtKB-SubCell
- spindle pole Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: HPA
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 28 | Y → A: Significant decrease in catalytic activity; when associated with A-31. 1 Publication | 1 | |
Mutagenesisi | 31 | L → A: Significant decrease in catalytic activity; when associated with A-28. 1 Publication | 1 | |
Mutagenesisi | 33 | N → D: Significant decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 97 | Y → A: 5-fold increase in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 97 | Y → F or L: Moderate increase in catalytic activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 140609 |
OpenTargetsi | ENSG00000151414 |
PharmGKBi | PA31551 |
Miscellaneous databases
Pharosi | Q8TDX7, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4849 |
DrugCentrali | Q8TDX7 |
Genetic variation databases
BioMutai | NEK7 |
DMDMi | 37537965 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086430 | 1 – 302 | Serine/threonine-protein kinase Nek7Add BLAST | 302 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 5 | PhosphoserineCombined sources | 1 | |
Modified residuei | 195 | Phosphoserine; by NEK91 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-1621 |
EPDi | Q8TDX7 |
jPOSTi | Q8TDX7 |
MassIVEi | Q8TDX7 |
MaxQBi | Q8TDX7 |
PaxDbi | Q8TDX7 |
PeptideAtlasi | Q8TDX7 |
PRIDEi | Q8TDX7 |
ProteomicsDBi | 74364 [Q8TDX7-1] 74365 [Q8TDX7-2] |
PTM databases
GlyGeni | Q8TDX7, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | Q8TDX7 |
MetOSitei | Q8TDX7 |
PhosphoSitePlusi | Q8TDX7 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000151414, Expressed in heart left ventricle and 251 other tissues |
ExpressionAtlasi | Q8TDX7, baseline and differential |
Genevisiblei | Q8TDX7, HS |
Organism-specific databases
HPAi | ENSG00000151414, Tissue enhanced (heart) |
Interactioni
Subunit structurei
Monomer (PubMed:21320329).
Interacts with NEK9 (PubMed:19414596).
Interacts with ANKS3; this interaction alters the subcellular distribution of NEK7 by preventing its nuclear translocation (PubMed:26188091).
Interacts (via N-terminus) with NLRP3 (via LRR repeat domain); the interaction is required for the formation of the complex NLRP3:PYCARD, oligomerization of PYCARD and activation of CASP1 (By similarity).
By similarity3 PublicationsBinary interactionsi
Q8TDX7
Isoform 1 [Q8TDX7-1]
With | #Exp. | IntAct |
---|---|---|
NLRP3 - isoform 2 [Q96P20-1] | 6 | EBI-16193799,EBI-14029575 |
Protein-protein interaction databases
BioGRIDi | 126636, 58 interactors |
DIPi | DIP-50780N |
IntActi | Q8TDX7, 96 interactors |
MINTi | Q8TDX7 |
STRINGi | 9606.ENSP00000356355 |
Chemistry databases
BindingDBi | Q8TDX7 |
Miscellaneous databases
RNActi | Q8TDX7, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q8TDX7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q8TDX7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 299 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 266 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 21 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 20 – 33 | NTE motifAdd BLAST | 14 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0591, Eukaryota |
GeneTreei | ENSGT00940000156725 |
HOGENOMi | CLU_000288_63_23_1 |
InParanoidi | Q8TDX7 |
OMAi | MSRMIKH |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q8TDX7 |
TreeFami | TF105135 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDEQSQGMQG PPVPQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR
60 70 80 90 100
AACLLDGVPV ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS
110 120 130 140 150
FIEDNELNIV LELADAGDLS RMIKHFKKQK RLIPERTVWK YFVQLCSALE
160 170 180 190 200
HMHSRRVMHR DIKPANVFIT ATGVVKLGDL GLGRFFSSKT TAAHSLVGTP
210 220 230 240 250
YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK MNLYSLCKKI
260 270 280 290 300
EQCDYPPLPS DHYSEELRQL VNMCINPDPE KRPDVTYVYD VAKRMHACTA
SS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8WAG2 | F8WAG2_HUMAN | Serine/threonine-protein kinase Nek... | NEK7 | 82 | Annotation score: | ||
F5H3U7 | F5H3U7_HUMAN | Serine/threonine-protein kinase Nek... | NEK7 | 62 | Annotation score: | ||
C9J1H8 | C9J1H8_HUMAN | Serine/threonine-protein kinase Nek... | NEK7 | 32 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_040924 | 35 | R → G1 PublicationCorresponds to variant dbSNP:rs55833332Ensembl. | 1 | |
Natural variantiVAR_040925 | 275 | I → M in an ovarian serous carcinoma sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_041243 | 67 – 119 | IFDLM…DAGDL → VCLLPVWERRVCALNACYFL EIIKGSESLQYMATLTNLFE NLPVSHHGSFAKK in isoform 2. 1 PublicationAdd BLAST | 53 | |
Alternative sequenceiVSP_041244 | 120 – 302 | Missing in isoform 2. 1 PublicationAdd BLAST | 183 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB062450 mRNA Translation: BAB85632.1 CK819069 mRNA No translation available. AC093419 Genomic DNA No translation available. AC093561 Genomic DNA No translation available. CH471067 Genomic DNA Translation: EAW91294.1 |
CCDSi | CCDS1394.1 [Q8TDX7-1] |
RefSeqi | NP_598001.1, NM_133494.2 [Q8TDX7-1] |
Genome annotation databases
Ensembli | ENST00000367383; ENSP00000356353; ENSG00000151414 [Q8TDX7-2] ENST00000367385; ENSP00000356355; ENSG00000151414 ENST00000538004; ENSP00000444621; ENSG00000151414 |
GeneIDi | 140609 |
KEGGi | hsa:140609 |
MANE-Selecti | ENST00000367385.9; ENSP00000356355.4; NM_133494.3; NP_598001.1 |
UCSCi | uc001gun.5, human [Q8TDX7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB062450 mRNA Translation: BAB85632.1 CK819069 mRNA No translation available. AC093419 Genomic DNA No translation available. AC093561 Genomic DNA No translation available. CH471067 Genomic DNA Translation: EAW91294.1 |
CCDSi | CCDS1394.1 [Q8TDX7-1] |
RefSeqi | NP_598001.1, NM_133494.2 [Q8TDX7-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2WQM | X-ray | 2.10 | A | 1-302 | [»] | |
2WQN | X-ray | 2.30 | A | 1-302 | [»] | |
5DE2 | X-ray | 2.78 | A/B | 1-302 | [»] | |
6NPY | electron microscopy | 3.80 | B | 1-302 | [»] | |
6S73 | X-ray | 3.50 | A/B/C/D | 1-302 | [»] | |
6S75 | X-ray | 3.30 | A/B/C/D | 1-302 | [»] | |
6S76 | X-ray | 3.38 | A/B/C/D | 1-302 | [»] | |
SMRi | Q8TDX7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 126636, 58 interactors |
DIPi | DIP-50780N |
IntActi | Q8TDX7, 96 interactors |
MINTi | Q8TDX7 |
STRINGi | 9606.ENSP00000356355 |
Chemistry databases
BindingDBi | Q8TDX7 |
ChEMBLi | CHEMBL4849 |
DrugCentrali | Q8TDX7 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
GlyGeni | Q8TDX7, 2 sites, 1 O-linked glycan (2 sites) |
iPTMneti | Q8TDX7 |
MetOSitei | Q8TDX7 |
PhosphoSitePlusi | Q8TDX7 |
Genetic variation databases
BioMutai | NEK7 |
DMDMi | 37537965 |
Proteomic databases
CPTACi | CPTAC-1621 |
EPDi | Q8TDX7 |
jPOSTi | Q8TDX7 |
MassIVEi | Q8TDX7 |
MaxQBi | Q8TDX7 |
PaxDbi | Q8TDX7 |
PeptideAtlasi | Q8TDX7 |
PRIDEi | Q8TDX7 |
ProteomicsDBi | 74364 [Q8TDX7-1] 74365 [Q8TDX7-2] |
Protocols and materials databases
Antibodypediai | 20630, 365 antibodies from 37 providers |
DNASUi | 140609 |
Genome annotation databases
Ensembli | ENST00000367383; ENSP00000356353; ENSG00000151414 [Q8TDX7-2] ENST00000367385; ENSP00000356355; ENSG00000151414 ENST00000538004; ENSP00000444621; ENSG00000151414 |
GeneIDi | 140609 |
KEGGi | hsa:140609 |
MANE-Selecti | ENST00000367385.9; ENSP00000356355.4; NM_133494.3; NP_598001.1 |
UCSCi | uc001gun.5, human [Q8TDX7-1] |
Organism-specific databases
CTDi | 140609 |
DisGeNETi | 140609 |
GeneCardsi | NEK7 |
HGNCi | HGNC:13386, NEK7 |
HPAi | ENSG00000151414, Tissue enhanced (heart) |
MIMi | 606848, gene |
neXtProti | NX_Q8TDX7 |
OpenTargetsi | ENSG00000151414 |
PharmGKBi | PA31551 |
VEuPathDBi | HostDB:ENSG00000151414 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0591, Eukaryota |
GeneTreei | ENSGT00940000156725 |
HOGENOMi | CLU_000288_63_23_1 |
InParanoidi | Q8TDX7 |
OMAi | MSRMIKH |
OrthoDBi | 1318335at2759 |
PhylomeDBi | Q8TDX7 |
TreeFami | TF105135 |
Enzyme and pathway databases
PathwayCommonsi | Q8TDX7 |
Reactomei | R-HSA-2980767, Activation of NIMA Kinases NEK9, NEK6, NEK7 R-HSA-3301854, Nuclear Pore Complex (NPC) Disassembly R-HSA-9648025, EML4 and NUDC in mitotic spindle formation |
SignaLinki | Q8TDX7 |
SIGNORi | Q8TDX7 |
Miscellaneous databases
BioGRID-ORCSi | 140609, 10 hits in 1079 CRISPR screens |
ChiTaRSi | NEK7, human |
EvolutionaryTracei | Q8TDX7 |
GenomeRNAii | 140609 |
Pharosi | Q8TDX7, Tchem |
PROi | PR:Q8TDX7 |
RNActi | Q8TDX7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000151414, Expressed in heart left ventricle and 251 other tissues |
ExpressionAtlasi | Q8TDX7, baseline and differential |
Genevisiblei | Q8TDX7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NEK7_HUMAN | |
Accessioni | Q8TDX7Primary (citable) accession number: Q8TDX7 Secondary accession number(s): A6NGT8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | June 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families