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Protein

Serine/threonine-protein kinase Nek7

Gene

NEK7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Activity regulationi

Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63ATP1
Sitei97Autoinhibitory1
Active sitei161Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 48ATP9

GO - Molecular functioni

GO - Biological processi

  • positive regulation of telomerase activity Source: BHF-UCL
  • positive regulation of telomere capping Source: BHF-UCL
  • positive regulation of telomere maintenance via telomerase Source: BHF-UCL
  • protein phosphorylation Source: UniProtKB
  • regulation of mitotic cell cycle Source: UniProtKB
  • spindle assembly Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
SignaLinkiQ8TDX7
SIGNORiQ8TDX7

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek7 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 7
Short name:
NimA-related protein kinase 7
Gene namesi
Name:NEK7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000151414.14
HGNCiHGNC:13386 NEK7
MIMi606848 gene
neXtProtiNX_Q8TDX7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi28Y → A: Significant decrease in catalytic activity; when associated with A-31. 1 Publication1
Mutagenesisi31L → A: Significant decrease in catalytic activity; when associated with A-28. 1 Publication1
Mutagenesisi33N → D: Significant decrease in catalytic activity. 1 Publication1
Mutagenesisi97Y → A: 5-fold increase in catalytic activity. 1 Publication1
Mutagenesisi97Y → F or L: Moderate increase in catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi140609
OpenTargetsiENSG00000151414
PharmGKBiPA31551

Chemistry databases

ChEMBLiCHEMBL4849

Polymorphism and mutation databases

BioMutaiNEK7
DMDMi37537965

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864301 – 302Serine/threonine-protein kinase Nek7Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei5PhosphoserineCombined sources1
Modified residuei195Phosphoserine; by NEK91 Publication1

Post-translational modificationi

Phosphorylation at Ser-195 required for its activation.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8TDX7
MaxQBiQ8TDX7
PaxDbiQ8TDX7
PeptideAtlasiQ8TDX7
PRIDEiQ8TDX7
ProteomicsDBi74364
74365 [Q8TDX7-2]

PTM databases

iPTMnetiQ8TDX7
PhosphoSitePlusiQ8TDX7

Expressioni

Tissue specificityi

Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.1 Publication

Gene expression databases

BgeeiENSG00000151414 Expressed in 241 organ(s), highest expression level in heart left ventricle
CleanExiHS_NEK7
ExpressionAtlasiQ8TDX7 baseline and differential
GenevisibleiQ8TDX7 HS

Organism-specific databases

HPAiHPA015520
HPA018193

Interactioni

Subunit structurei

Monomer. Interacts with NEK9.2 Publications

Protein-protein interaction databases

BioGridi126636, 37 interactors
DIPiDIP-50780N
IntActiQ8TDX7, 13 interactors
STRINGi9606.ENSP00000356355

Chemistry databases

BindingDBiQ8TDX7

Structurei

Secondary structure

1302
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8TDX7
SMRiQ8TDX7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TDX7

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 299Protein kinasePROSITE-ProRule annotationAdd BLAST266

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 33NTE motifAdd BLAST14

Domaini

Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.1 Publication
The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0591 Eukaryota
ENOG410XNQP LUCA
GeneTreeiENSGT00760000118997
HOGENOMiHOG000233029
HOVERGENiHBG105886
InParanoidiQ8TDX7
KOiK20876
OMAiQLVNICI
OrthoDBiEOG091G0AER
PhylomeDBiQ8TDX7
TreeFamiTF105135

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8TDX7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDEQSQGMQG PPVPQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR
60 70 80 90 100
AACLLDGVPV ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS
110 120 130 140 150
FIEDNELNIV LELADAGDLS RMIKHFKKQK RLIPERTVWK YFVQLCSALE
160 170 180 190 200
HMHSRRVMHR DIKPANVFIT ATGVVKLGDL GLGRFFSSKT TAAHSLVGTP
210 220 230 240 250
YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK MNLYSLCKKI
260 270 280 290 300
EQCDYPPLPS DHYSEELRQL VNMCINPDPE KRPDVTYVYD VAKRMHACTA

SS
Length:302
Mass (Da):34,551
Last modified:June 1, 2002 - v1
Checksum:iC1FC990B34D94D4C
GO
Isoform 2 (identifier: Q8TDX7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-119: IFDLMDAKAR...VLELADAGDL → VCLLPVWERR...VSHHGSFAKK
     120-302: Missing.

Note: No experimental confirmation available.
Show »
Length:119
Mass (Da):13,402
Checksum:iC5CF3EAC5976AE9F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WAG2F8WAG2_HUMAN
Serine/threonine-protein kinase Nek...
NEK7
82Annotation score:
F5H3U7F5H3U7_HUMAN
Serine/threonine-protein kinase Nek...
NEK7
62Annotation score:
C9J1H8C9J1H8_HUMAN
Serine/threonine-protein kinase Nek...
NEK7
32Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04092435R → G1 PublicationCorresponds to variant dbSNP:rs55833332Ensembl.1
Natural variantiVAR_040925275I → M in an ovarian serous carcinoma sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04124367 – 119IFDLM…DAGDL → VCLLPVWERRVCALNACYFL EIIKGSESLQYMATLTNLFE NLPVSHHGSFAKK in isoform 2. 1 PublicationAdd BLAST53
Alternative sequenceiVSP_041244120 – 302Missing in isoform 2. 1 PublicationAdd BLAST183

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB062450 mRNA Translation: BAB85632.1
CK819069 mRNA No translation available.
AC093419 Genomic DNA No translation available.
AC093561 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW91294.1
CCDSiCCDS1394.1 [Q8TDX7-1]
RefSeqiNP_598001.1, NM_133494.2 [Q8TDX7-1]
UniGeneiHs.24119
Hs.745083

Genome annotation databases

EnsembliENST00000367383; ENSP00000356353; ENSG00000151414 [Q8TDX7-2]
ENST00000367385; ENSP00000356355; ENSG00000151414 [Q8TDX7-1]
ENST00000538004; ENSP00000444621; ENSG00000151414 [Q8TDX7-1]
GeneIDi140609
KEGGihsa:140609
UCSCiuc001gun.5 human [Q8TDX7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB062450 mRNA Translation: BAB85632.1
CK819069 mRNA No translation available.
AC093419 Genomic DNA No translation available.
AC093561 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW91294.1
CCDSiCCDS1394.1 [Q8TDX7-1]
RefSeqiNP_598001.1, NM_133494.2 [Q8TDX7-1]
UniGeneiHs.24119
Hs.745083

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WQMX-ray2.10A1-302[»]
2WQNX-ray2.30A1-302[»]
5DE2X-ray2.78A/B1-302[»]
6GT1X-ray3.50A/B/C/D1-302[»]
ProteinModelPortaliQ8TDX7
SMRiQ8TDX7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126636, 37 interactors
DIPiDIP-50780N
IntActiQ8TDX7, 13 interactors
STRINGi9606.ENSP00000356355

Chemistry databases

BindingDBiQ8TDX7
ChEMBLiCHEMBL4849

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiQ8TDX7
PhosphoSitePlusiQ8TDX7

Polymorphism and mutation databases

BioMutaiNEK7
DMDMi37537965

Proteomic databases

EPDiQ8TDX7
MaxQBiQ8TDX7
PaxDbiQ8TDX7
PeptideAtlasiQ8TDX7
PRIDEiQ8TDX7
ProteomicsDBi74364
74365 [Q8TDX7-2]

Protocols and materials databases

DNASUi140609
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367383; ENSP00000356353; ENSG00000151414 [Q8TDX7-2]
ENST00000367385; ENSP00000356355; ENSG00000151414 [Q8TDX7-1]
ENST00000538004; ENSP00000444621; ENSG00000151414 [Q8TDX7-1]
GeneIDi140609
KEGGihsa:140609
UCSCiuc001gun.5 human [Q8TDX7-1]

Organism-specific databases

CTDi140609
DisGeNETi140609
EuPathDBiHostDB:ENSG00000151414.14
GeneCardsiNEK7
HGNCiHGNC:13386 NEK7
HPAiHPA015520
HPA018193
MIMi606848 gene
neXtProtiNX_Q8TDX7
OpenTargetsiENSG00000151414
PharmGKBiPA31551
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0591 Eukaryota
ENOG410XNQP LUCA
GeneTreeiENSGT00760000118997
HOGENOMiHOG000233029
HOVERGENiHBG105886
InParanoidiQ8TDX7
KOiK20876
OMAiQLVNICI
OrthoDBiEOG091G0AER
PhylomeDBiQ8TDX7
TreeFamiTF105135

Enzyme and pathway databases

ReactomeiR-HSA-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7
R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly
SignaLinkiQ8TDX7
SIGNORiQ8TDX7

Miscellaneous databases

ChiTaRSiNEK7 human
EvolutionaryTraceiQ8TDX7
GenomeRNAii140609
PROiPR:Q8TDX7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000151414 Expressed in 241 organ(s), highest expression level in heart left ventricle
CleanExiHS_NEK7
ExpressionAtlasiQ8TDX7 baseline and differential
GenevisibleiQ8TDX7 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR00109 TYRKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNEK7_HUMAN
AccessioniPrimary (citable) accession number: Q8TDX7
Secondary accession number(s): A6NGT8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: June 1, 2002
Last modified: October 10, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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