UniProtKB - Q8TDD1 (DDX54_HUMAN)
Protein
ATP-dependent RNA helicase DDX54
Gene
DDX54
Organism
Homo sapiens (Human)
Status
Functioni
Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.1 Publication
Catalytic activityi
- EC:3.6.4.13
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 140 – 147 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- estrogen receptor binding Source: UniProtKB
- RNA binding Source: UniProtKB
- RNA helicase activity Source: UniProtKB
- signaling receptor binding Source: UniProtKB
- transcription corepressor activity Source: UniProtKB
GO - Biological processi
- intracellular estrogen receptor signaling pathway Source: UniProtKB
- RNA metabolic process Source: UniProtKB
- RNA processing Source: UniProtKB
- rRNA processing Source: GO_Central
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q8TDD1 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DDX54 (EC:3.6.4.13)Alternative name(s): ATP-dependent RNA helicase DP97 DEAD box RNA helicase 97 kDa DEAD box protein 54 |
Gene namesi | Name:DDX54 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000123064.12 |
HGNCi | HGNC:20084, DDX54 |
MIMi | 611665, gene |
neXtProti | NX_Q8TDD1 |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
Golgi apparatus
- Golgi apparatus Source: HPA
Nucleus
- nucleolus Source: UniProtKB
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- membrane Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 79039 |
OpenTargetsi | ENSG00000123064 |
PharmGKBi | PA134992026 |
Miscellaneous databases
Pharosi | Q8TDD1, Tbio |
Polymorphism and mutation databases
BioMutai | DDX54 |
DMDMi | 46576615 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055056 | 1 – 881 | ATP-dependent RNA helicase DDX54Add BLAST | 881 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | PhosphoserineCombined sources | 1 | |
Modified residuei | 39 | PhosphoserineCombined sources | 1 | |
Modified residuei | 41 | PhosphoserineCombined sources | 1 | |
Modified residuei | 75 | PhosphoserineCombined sources | 1 | |
Modified residuei | 644 | PhosphoserineCombined sources | 1 | |
Modified residuei | 696 | PhosphoserineCombined sources | 1 | |
Modified residuei | 698 | PhosphoserineCombined sources | 1 | |
Modified residuei | 782 | PhosphoserineCombined sources | 1 | |
Modified residuei | 788 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8TDD1 |
jPOSTi | Q8TDD1 |
MassIVEi | Q8TDD1 |
MaxQBi | Q8TDD1 |
PaxDbi | Q8TDD1 |
PeptideAtlasi | Q8TDD1 |
PRIDEi | Q8TDD1 |
ProteomicsDBi | 74267 [Q8TDD1-1] 74268 [Q8TDD1-2] |
2D gel databases
SWISS-2DPAGEi | Q8TDD1 |
PTM databases
iPTMneti | Q8TDD1 |
PhosphoSitePlusi | Q8TDD1 |
SwissPalmi | Q8TDD1 |
Expressioni
Tissue specificityi
Expressed in the fallopian tube, cervix and uterus. Also expressed in the brain.1 Publication
Gene expression databases
Bgeei | ENSG00000123064, Expressed in layer of synovial tissue and 215 other tissues |
ExpressionAtlasi | Q8TDD1, baseline and differential |
Genevisiblei | Q8TDD1, HS |
Organism-specific databases
HPAi | ENSG00000123064, Low tissue specificity |
Interactioni
Subunit structurei
Interacts in a hormone-dependent manner with nuclear receptors.
GO - Molecular functioni
- estrogen receptor binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 122503, 150 interactors |
IntActi | Q8TDD1, 74 interactors |
MINTi | Q8TDD1 |
STRINGi | 9606.ENSP00000323858 |
Miscellaneous databases
RNActi | Q8TDD1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 127 – 299 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 173 | |
Domaini | 326 – 473 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 148 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 605 – 647 | Interaction with nuclear receptorsAdd BLAST | 43 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 96 – 124 | Q motifAdd BLAST | 29 | |
Motifi | 247 – 250 | DEAD box | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0337, Eukaryota |
GeneTreei | ENSGT00550000075100 |
HOGENOMi | CLU_003041_5_2_1 |
InParanoidi | Q8TDD1 |
OMAi | CVEMDLK |
OrthoDBi | 268859at2759 |
PhylomeDBi | Q8TDD1 |
TreeFami | TF105707 |
Family and domain databases
InterProi | View protein in InterPro IPR012541, DBP10_C IPR033517, DDX54/DBP10 IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
PANTHERi | PTHR24031:SF292, PTHR24031:SF292, 1 hit |
Pfami | View protein in Pfam PF08147, DBP10CT, 1 hit PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM01123, DBP10CT, 1 hit SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8TDD1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA
60 70 80 90 100
EDDARARKLG PGRPLPTFPT SECTSDVEPD TREMVRAQNK KKKKSGGFQS
110 120 130 140 150
MGLSYPVFKG IMKKGYKVPT PIQRKTIPVI LDGKDVVAMA RTGSGKTACF
160 170 180 190 200
LLPMFERLKT HSAQTGARAL ILSPTRELAL QTLKFTKELG KFTGLKTALI
210 220 230 240 250
LGGDRMEDQF AALHENPDII IATPGRLVHV AVEMSLKLQS VEYVVFDEAD
260 270 280 290 300
RLFEMGFAEQ LQEIIARLPG GHQTVLFSAT LPKLLVEFAR AGLTEPVLIR
310 320 330 340 350
LDVDTKLNEQ LKTSFFLVRE DTKAAVLLHL LHNVVRPQDQ TVVFVATKHH
360 370 380 390 400
AEYLTELLTT QRVSCAHIYS ALDPTARKIN LAKFTLGKCS TLIVTDLAAR
410 420 430 440 450
GLDIPLLDNV INYSFPAKGK LFLHRVGRVA RAGRSGTAYS LVAPDEIPYL
460 470 480 490 500
LDLHLFLGRS LTLARPLKEP SGVAGVDGML GRVPQSVVDE EDSGLQSTLE
510 520 530 540 550
ASLELRGLAR VADNAQQQYV RSRPAPSPES IKRAKEMDLV GLGLHPLFSS
560 570 580 590 600
RFEEEELQRL RLVDSIKNYR SRATIFEINA SSRDLCSQVM RAKRQKDRKA
610 620 630 640 650
IARFQQGQQG RQEQQEGPVG PAPSRPALQE KQPEKEEEEE AGESVEDIFS
660 670 680 690 700
EVVGRKRQRS GPNRGAKRRR EEARQRDQEF YIPYRPKDFD SERGLSISGE
710 720 730 740 750
GGAFEQQAAG AVLDLMGDEA QNLTRGRQQL KWDRKKKRFV GQSGQEDKKK
760 770 780 790 800
IKTESGRYIS SSYKRDLYQK WKQKQKIDDR DSDEEGASDR RGPERRGGKR
810 820 830 840 850
DRGQGASRPH APGTPAGRVR PELKTKQQIL KQRRRAQKLH FLQRGGLKQL
860 870 880
SARNRRRVQE LQQGAFGRGA RSKKGKMRKR M
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VRX4 | F8VRX4_HUMAN | ATP-dependent RNA helicase DDX54 | DDX54 | 208 | Annotation score: | ||
H0YHH7 | H0YHH7_HUMAN | ATP-dependent RNA helicase DDX54 | DDX54 | 160 | Annotation score: | ||
H0YHZ9 | H0YHZ9_HUMAN | ATP-dependent RNA helicase DDX54 | DDX54 | 82 | Annotation score: |
Sequence cautioni
The sequence AAN59978 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_083615 | 20 | W → R Found in a patient with a neurodevelopmental disorder; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs774035439EnsemblClinVar. | 1 | |
Natural variantiVAR_083616 | 216 | N → S Found in a patient with a neurodevelopmental disorder; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs764707570EnsemblClinVar. | 1 | |
Natural variantiVAR_083617 | 286 | V → M Found in a patient with a neurodevelopmental disorder; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs148961522EnsemblClinVar. | 1 | |
Natural variantiVAR_083618 | 298 | L → F Found in a patient with a neurodevelopmental disorder; unknown pathological significance. 1 Publication | 1 | |
Natural variantiVAR_052171 | 570 | R → H. Corresponds to variant dbSNP:rs35519289Ensembl. | 1 | |
Natural variantiVAR_083619 | 611 | R → Q Found in a patient with a neurodevelopmental disorder; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs777276705EnsemblClinVar. | 1 | |
Natural variantiVAR_052172 | 693 | R → Q. Corresponds to variant dbSNP:rs11564Ensembl. | 1 | |
Natural variantiVAR_052173 | 712 | V → A. Corresponds to variant dbSNP:rs10354Ensembl. | 1 | |
Natural variantiVAR_033860 | 821 | P → L. Corresponds to variant dbSNP:rs1048889Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040135 | 804 | Q → QA in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF478457 mRNA Translation: AAL85336.1 AC089999 Genomic DNA No translation available. AY148094 mRNA Translation: AAN59978.1 Sequence problems. BC005848 mRNA Translation: AAH05848.2 |
CCDSi | CCDS31907.1 [Q8TDD1-1] CCDS44984.1 [Q8TDD1-2] |
RefSeqi | NP_001104792.1, NM_001111322.1 [Q8TDD1-2] NP_076977.3, NM_024072.3 [Q8TDD1-1] |
Genome annotation databases
Ensembli | ENST00000306014; ENSP00000304072; ENSG00000123064 [Q8TDD1-1] ENST00000314045; ENSP00000323858; ENSG00000123064 [Q8TDD1-2] |
GeneIDi | 79039 |
KEGGi | hsa:79039 |
UCSCi | uc001tup.4, human [Q8TDD1-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF478457 mRNA Translation: AAL85336.1 AC089999 Genomic DNA No translation available. AY148094 mRNA Translation: AAN59978.1 Sequence problems. BC005848 mRNA Translation: AAH05848.2 |
CCDSi | CCDS31907.1 [Q8TDD1-1] CCDS44984.1 [Q8TDD1-2] |
RefSeqi | NP_001104792.1, NM_001111322.1 [Q8TDD1-2] NP_076977.3, NM_024072.3 [Q8TDD1-1] |
3D structure databases
SMRi | Q8TDD1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122503, 150 interactors |
IntActi | Q8TDD1, 74 interactors |
MINTi | Q8TDD1 |
STRINGi | 9606.ENSP00000323858 |
PTM databases
iPTMneti | Q8TDD1 |
PhosphoSitePlusi | Q8TDD1 |
SwissPalmi | Q8TDD1 |
Polymorphism and mutation databases
BioMutai | DDX54 |
DMDMi | 46576615 |
2D gel databases
SWISS-2DPAGEi | Q8TDD1 |
Proteomic databases
EPDi | Q8TDD1 |
jPOSTi | Q8TDD1 |
MassIVEi | Q8TDD1 |
MaxQBi | Q8TDD1 |
PaxDbi | Q8TDD1 |
PeptideAtlasi | Q8TDD1 |
PRIDEi | Q8TDD1 |
ProteomicsDBi | 74267 [Q8TDD1-1] 74268 [Q8TDD1-2] |
Protocols and materials databases
Antibodypediai | 18719, 154 antibodies |
DNASUi | 79039 |
Genome annotation databases
Ensembli | ENST00000306014; ENSP00000304072; ENSG00000123064 [Q8TDD1-1] ENST00000314045; ENSP00000323858; ENSG00000123064 [Q8TDD1-2] |
GeneIDi | 79039 |
KEGGi | hsa:79039 |
UCSCi | uc001tup.4, human [Q8TDD1-1] |
Organism-specific databases
CTDi | 79039 |
DisGeNETi | 79039 |
EuPathDBi | HostDB:ENSG00000123064.12 |
GeneCardsi | DDX54 |
HGNCi | HGNC:20084, DDX54 |
HPAi | ENSG00000123064, Low tissue specificity |
MIMi | 611665, gene |
neXtProti | NX_Q8TDD1 |
OpenTargetsi | ENSG00000123064 |
PharmGKBi | PA134992026 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0337, Eukaryota |
GeneTreei | ENSGT00550000075100 |
HOGENOMi | CLU_003041_5_2_1 |
InParanoidi | Q8TDD1 |
OMAi | CVEMDLK |
OrthoDBi | 268859at2759 |
PhylomeDBi | Q8TDD1 |
TreeFami | TF105707 |
Enzyme and pathway databases
PathwayCommonsi | Q8TDD1 |
Miscellaneous databases
BioGRID-ORCSi | 79039, 722 hits in 851 CRISPR screens |
ChiTaRSi | DDX54, human |
GenomeRNAii | 79039 |
Pharosi | Q8TDD1, Tbio |
PROi | PR:Q8TDD1 |
RNActi | Q8TDD1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000123064, Expressed in layer of synovial tissue and 215 other tissues |
ExpressionAtlasi | Q8TDD1, baseline and differential |
Genevisiblei | Q8TDD1, HS |
Family and domain databases
InterProi | View protein in InterPro IPR012541, DBP10_C IPR033517, DDX54/DBP10 IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
PANTHERi | PTHR24031:SF292, PTHR24031:SF292, 1 hit |
Pfami | View protein in Pfam PF08147, DBP10CT, 1 hit PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM01123, DBP10CT, 1 hit SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDX54_HUMAN | |
Accessioni | Q8TDD1Primary (citable) accession number: Q8TDD1 Secondary accession number(s): Q86YT8, Q9BRZ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | April 26, 2004 | |
Last modified: | December 2, 2020 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations