Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein

Gene

PREX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • GTPase activator activity Source: UniProtKB
  • guanyl-nucleotide exchange factor activity Source: Reactome
  • phospholipid binding Source: UniProtKB
  • Rac guanyl-nucleotide exchange factor activity Source: GO_Central
  • Rho guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-HSA-193648 NRAGE signals death through JNK
R-HSA-194840 Rho GTPase cycle
R-HSA-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
Short name:
P-Rex1
Short name:
PtdIns(3,4,5)-dependent Rac exchanger 1
Gene namesi
Name:PREX1
Synonyms:KIAA1415
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000124126.13
HGNCiHGNC:32594 PREX1
MIMi606905 gene
neXtProtiNX_Q8TCU6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi57580
OpenTargetsiENSG00000124126
PharmGKBiPA164725018

Polymorphism and mutation databases

BioMutaiPREX1
DMDMi148886999

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809651 – 1659Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 proteinAdd BLAST1659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei319PhosphoserineCombined sources1
Modified residuei1001PhosphoserineBy similarity1
Modified residuei1195PhosphoserineBy similarity1
Modified residuei1200PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8TCU6
MaxQBiQ8TCU6
PaxDbiQ8TCU6
PeptideAtlasiQ8TCU6
PRIDEiQ8TCU6
ProteomicsDBi74175
74176 [Q8TCU6-2]
74177 [Q8TCU6-3]

PTM databases

iPTMnetiQ8TCU6
PhosphoSitePlusiQ8TCU6

Expressioni

Tissue specificityi

Mainly expressed in peripheral blood leukocytes and brain. Expressed at intermediate level in spleen and lymph nodes, and weakly expressed in other tissues.

Gene expression databases

BgeeiENSG00000124126 Expressed in 190 organ(s), highest expression level in blood
ExpressionAtlasiQ8TCU6 baseline and differential
GenevisibleiQ8TCU6 HS

Organism-specific databases

HPAiHPA001927

Interactioni

Subunit structurei

Interacts preferentially with RAC2. Interacts with RAC1. Interacts with AUTS2.By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121633, 9 interactors
DIPiDIP-46975N
IntActiQ8TCU6, 13 interactors
STRINGi9606.ENSP00000361009

Structurei

Secondary structure

11659
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8TCU6
SMRiQ8TCU6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8TCU6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 240DHPROSITE-ProRule annotationAdd BLAST192
Domaini271 – 392PHPROSITE-ProRule annotationAdd BLAST122
Domaini421 – 496DEP 1PROSITE-ProRule annotationAdd BLAST76
Domaini523 – 597DEP 2PROSITE-ProRule annotationAdd BLAST75
Domaini625 – 703PDZPROSITE-ProRule annotationAdd BLAST79

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410ITRX Eukaryota
ENOG410Z6ZS LUCA
GeneTreeiENSGT00930000150835
HOVERGENiHBG053677
InParanoidiQ8TCU6
KOiK12365
OMAiMPQYEFR
OrthoDBiEOG091G00MJ
PhylomeDBiQ8TCU6
TreeFamiTF328639

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
Gene3Di1.10.10.10, 2 hits
1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000591 DEP_dom
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR001478 PDZ
IPR036034 PDZ_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00610 DEP, 2 hits
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00049 DEP, 2 hits
SM00228 PDZ, 2 hits
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
SSF48065 SSF48065, 1 hit
SSF50156 SSF50156, 2 hits
PROSITEiView protein in PROSITE
PS50186 DEP, 2 hits
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50106 PDZ, 1 hit
PS50003 PH_DOMAIN, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8TCU6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEAPSGSEPG GDGAGDCAHP DPRAPGAAAP SSGPGPCAAA RESERQLRLR
60 70 80 90 100
LCVLNEILGT ERDYVGTLRF LQSAFLHRIR QNVADSVEKG LTEENVKVLF
110 120 130 140 150
SNIEDILEVH KDFLAALEYC LHPEPQSQHE LGNVFLKFKD KFCVYEEYCS
160 170 180 190 200
NHEKALRLLV ELNKIPTVRA FLLSCMLLGG RKTTDIPLEG YLLSPIQRIC
210 220 230 240 250
KYPLLLKELA KRTPGKHPDH PAVQSALQAM KTVCSNINET KRQMEKLEAL
260 270 280 290 300
EQLQSHIEGW EGSNLTDICT QLLLQGTLLK ISAGNIQERA FFLFDNLLVY
310 320 330 340 350
CKRKSRVTGS KKSTKRTKSI NGSLYIFRGR INTEVMEVEN VEDGTADYHS
360 370 380 390 400
NGYTVTNGWK IHNTAKNKWF VCMAKTAEEK QKWLDAIIRE REQRESLKLG
410 420 430 440 450
MERDAYVMIA EKGEKLYHMM MNKKVNLIKD RRRKLSTVPK CFLGNEFVAW
460 470 480 490 500
LLEIGEISKT EEGVNLGQAL LENGIIHHVS DKHQFKNEQV MYRFRYDDGT
510 520 530 540 550
YKARSELEDI MSKGVRLYCR LHSLYTPVIK DRDYHLKTYK SVLPGSKLVD
560 570 580 590 600
WLLAQGDCQT REEAVALGVG LCNNGFMHHV LEKSEFRDES QYFRFHADEE
610 620 630 640 650
MEGTSSKNKQ LRNDFKLVEN ILAKRLLILP QEEDYGFDIE EKNKAVVVKS
660 670 680 690 700
VQRGSLAEVA GLQVGRKIYS INEDLVFLRP FSEVESILNQ SFCSRRPLRL
710 720 730 740 750
LVATKAKEII KIPDQPDTLC FQIRGAAPPY VYAVGRGSEA MAAGLCAGQC
760 770 780 790 800
ILKVNGSNVM NDGAPEVLEH FQAFRSRREE ALGLYQWIYH THEDAQEARA
810 820 830 840 850
SQEASTEDPS GEQAQEEDQA DSAFPLLSLG PRLSLCEDSP MVTLTVDNVH
860 870 880 890 900
LEHGVVYEYV STAGVRCHVL EKIVEPRGCF GLTAKILEAF AANDSVFVEN
910 920 930 940 950
CRRLMALSSA IVTMPHFEFR NICDTKLESI GQRIACYQEF AAQLKSRVSP
960 970 980 990 1000
PFKQAPLEPH PLCGLDFCPT NCHINLMEVS YPKTTPSVGR SFSIRFGRKP
1010 1020 1030 1040 1050
SLIGLDPEQG HLNPMSYTQH CITTMAAPSW KCLPAAEGDP QGQGLHDGSF
1060 1070 1080 1090 1100
GPASGTLGQE DRGLSFLLKQ EDREIQDAYL QLFTKLDVAL KEMKQYVTQI
1110 1120 1130 1140 1150
NRLLSTITEP TSGGSCDASL AEEASSLPLV SEESEMDRSD HGGIKKVCFK
1160 1170 1180 1190 1200
VAEEDQEDSG HDTMSYRDSY SECNSNRDSV LSYTSVRSNS SYLGSDEMGS
1210 1220 1230 1240 1250
GDELPCDMRI PSDKQDKLHG CLEHLFNQVD SINALLKGPV MSRAFEETKH
1260 1270 1280 1290 1300
FPMNHSLQEF KQKEECTIRG RSLIQISIQE DPWNLPNSIK TLVDNIQRYV
1310 1320 1330 1340 1350
EDGKNQLLLA LLKCTDTELQ LRRDAIFCQA LVAAVCTFSK QLLAALGYRY
1360 1370 1380 1390 1400
NNNGEYEESS RDASRKWLEQ VAATGVLLHC QSLLSPATVK EERTMLEDIW
1410 1420 1430 1440 1450
VTLSELDNVT FSFKQLDENY VANTNVFYHI EGSRQALKVI FYLDSYHFSK
1460 1470 1480 1490 1500
LPSRLEGGAS LRLHTALFTK VLENVEGLPS PGSQAAEDLQ QDINAQSLEK
1510 1520 1530 1540 1550
VQQYYRKLRA FYLERSNLPT DASTTAVKID QLIRPINALD ELCRLMKSFV
1560 1570 1580 1590 1600
HPKPGAAGSV GAGLIPISSE LCYRLGACQM VMCGTGMQRS TLSVSLEQAA
1610 1620 1630 1640 1650
ILARSHGLLP KCIMQATDIM RKQGPRVEIL AKNLRVKDQM PQGAPRLYRL

CQPPVDGDL
Length:1,659
Mass (Da):186,203
Last modified:May 29, 2007 - v3
Checksum:i159419355ED0F00F
GO
Isoform 2 (identifier: Q8TCU6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-703: Missing.
     704-708: TKAKE → MGLEQ

Show »
Length:956
Mass (Da):106,093
Checksum:iE2DCA9099F0CE2C7
GO
Isoform 3 (identifier: Q8TCU6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1471-1561: VLENVEGLPS...PKPGAAGSVG → GELQAWGRED...TCPRMPAPRR
     1562-1659: Missing.

Note: Gene prediction based on EST data. No experimental confirmation available.
Show »
Length:1,561
Mass (Da):175,847
Checksum:iCACDE55657E3A86D
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YDZ4H0YDZ4_HUMAN
Phosphatidylinositol 3,4,5-trisphos...
PREX1
883Annotation score:
A0A087WY11A0A087WY11_HUMAN
Phosphatidylinositol 3,4,5-trisphos...
PREX1
12Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti910A → V in CAB66514 (PubMed:11230166).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061798659V → M1 PublicationCorresponds to variant dbSNP:rs55904123Ensembl.1
Natural variantiVAR_057191696R → C. Corresponds to variant dbSNP:rs7271583Ensembl.1
Natural variantiVAR_061799786Q → H1 PublicationCorresponds to variant dbSNP:rs41283558Ensembl.1
Natural variantiVAR_0571921118A → T1 PublicationCorresponds to variant dbSNP:rs6012504Ensembl.1
Natural variantiVAR_0232101240V → I1 PublicationCorresponds to variant dbSNP:rs16993997Ensembl.1
Natural variantiVAR_0232111340K → E5 PublicationsCorresponds to variant dbSNP:rs2664521Ensembl.1
Natural variantiVAR_0232121559S → T. Corresponds to variant dbSNP:rs3936192Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0018181 – 703Missing in isoform 2. 1 PublicationAdd BLAST703
Alternative sequenceiVSP_001819704 – 708TKAKE → MGLEQ in isoform 2. 1 Publication5
Alternative sequenceiVSP_0150901471 – 1561VLENV…AGSVG → GELQAWGREDHGGRAKATKH TGSQPPARPRHLPSQCWRTW RGCLLQAARPRRICSRTSTR SPWRKFSSITANSGHFTWSG LTCPRMPAPRR in isoform 3. CuratedAdd BLAST91
Alternative sequenceiVSP_0150911562 – 1659Missing in isoform 3. CuratedAdd BLAST98

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ320261 mRNA Translation: CAC86401.1
AL136579 mRNA Translation: CAB66514.1
AL035106 Genomic DNA No translation available.
AL133342 Genomic DNA No translation available.
AL445192 Genomic DNA No translation available.
AB037836 mRNA Translation: BAA92653.2
CH471077 Genomic DNA Translation: EAW75684.1
CH471077 Genomic DNA Translation: EAW75685.1
BC053616 mRNA Translation: AAH53616.1
AL832913 mRNA Translation: CAH10614.1
CCDSiCCDS13410.1 [Q8TCU6-1]
RefSeqiNP_065871.2, NM_020820.3
UniGeneiHs.153310

Genome annotation databases

EnsembliENST00000371941; ENSP00000361009; ENSG00000124126 [Q8TCU6-1]
GeneIDi57580
KEGGihsa:57580
UCSCiuc002xtw.2 human [Q8TCU6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ320261 mRNA Translation: CAC86401.1
AL136579 mRNA Translation: CAB66514.1
AL035106 Genomic DNA No translation available.
AL133342 Genomic DNA No translation available.
AL445192 Genomic DNA No translation available.
AB037836 mRNA Translation: BAA92653.2
CH471077 Genomic DNA Translation: EAW75684.1
CH471077 Genomic DNA Translation: EAW75685.1
BC053616 mRNA Translation: AAH53616.1
AL832913 mRNA Translation: CAH10614.1
CCDSiCCDS13410.1 [Q8TCU6-1]
RefSeqiNP_065871.2, NM_020820.3
UniGeneiHs.153310

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QIKX-ray2.28A607-706[»]
4YONX-ray1.95A1-404[»]
5D27X-ray1.92A245-408[»]
5D3VX-ray1.85A/B245-408[»]
5D3WX-ray1.85A/B245-408[»]
5D3XX-ray1.69A/B245-408[»]
5D3YX-ray1.95A/B245-408[»]
5FI0X-ray3.28A/C/E/G38-408[»]
5FI1X-ray3.20A38-408[»]
ProteinModelPortaliQ8TCU6
SMRiQ8TCU6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121633, 9 interactors
DIPiDIP-46975N
IntActiQ8TCU6, 13 interactors
STRINGi9606.ENSP00000361009

PTM databases

iPTMnetiQ8TCU6
PhosphoSitePlusiQ8TCU6

Polymorphism and mutation databases

BioMutaiPREX1
DMDMi148886999

Proteomic databases

EPDiQ8TCU6
MaxQBiQ8TCU6
PaxDbiQ8TCU6
PeptideAtlasiQ8TCU6
PRIDEiQ8TCU6
ProteomicsDBi74175
74176 [Q8TCU6-2]
74177 [Q8TCU6-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371941; ENSP00000361009; ENSG00000124126 [Q8TCU6-1]
GeneIDi57580
KEGGihsa:57580
UCSCiuc002xtw.2 human [Q8TCU6-1]

Organism-specific databases

CTDi57580
DisGeNETi57580
EuPathDBiHostDB:ENSG00000124126.13
GeneCardsiPREX1
H-InvDBiHIX0138059
HGNCiHGNC:32594 PREX1
HPAiHPA001927
MIMi606905 gene
neXtProtiNX_Q8TCU6
OpenTargetsiENSG00000124126
PharmGKBiPA164725018
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITRX Eukaryota
ENOG410Z6ZS LUCA
GeneTreeiENSGT00930000150835
HOVERGENiHBG053677
InParanoidiQ8TCU6
KOiK12365
OMAiMPQYEFR
OrthoDBiEOG091G00MJ
PhylomeDBiQ8TCU6
TreeFamiTF328639

Enzyme and pathway databases

ReactomeiR-HSA-193648 NRAGE signals death through JNK
R-HSA-194840 Rho GTPase cycle
R-HSA-416482 G alpha (12/13) signalling events

Miscellaneous databases

ChiTaRSiPREX1 human
EvolutionaryTraceiQ8TCU6
GeneWikiiPREX1
GenomeRNAii57580
PROiPR:Q8TCU6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000124126 Expressed in 190 organ(s), highest expression level in blood
ExpressionAtlasiQ8TCU6 baseline and differential
GenevisibleiQ8TCU6 HS

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
Gene3Di1.10.10.10, 2 hits
1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000591 DEP_dom
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR001478 PDZ
IPR036034 PDZ_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00610 DEP, 2 hits
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00049 DEP, 2 hits
SM00228 PDZ, 2 hits
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits
SSF48065 SSF48065, 1 hit
SSF50156 SSF50156, 2 hits
PROSITEiView protein in PROSITE
PS50186 DEP, 2 hits
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50106 PDZ, 1 hit
PS50003 PH_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPREX1_HUMAN
AccessioniPrimary (citable) accession number: Q8TCU6
Secondary accession number(s): E1P5X9
, Q5JS95, Q5JS96, Q69YL2, Q7Z2L9, Q9BQH0, Q9BX55, Q9H4Q6, Q9P2D2, Q9UGQ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: May 29, 2007
Last modified: November 7, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again