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Protein

Phosphoethanolamine/phosphocholine phosphatase

Gene

PHOSPHO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.

Catalytic activityi

O-phosphoethanolamine + H2O = ethanolamine + phosphate.1 Publication
Phosphocholine + H2O = choline + phosphate.1 Publication

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=3 µM for PEA1 Publication
  2. KM=11.4 µM for PCho1 Publication

    pH dependencei

    Optimum pH is 6.7.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei32NucleophileCurated1
    Metal bindingi32MagnesiumCurated1
    Active sitei34Proton donorBy similarity1
    Metal bindingi34MagnesiumBy similarity1
    Binding sitei43SubstrateCurated1
    Binding sitei123SubstrateCurated1
    Metal bindingi203MagnesiumCurated1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processMineral balance
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS16266-MONOMER
    BRENDAi3.1.3.75 2681
    ReactomeiR-HSA-1483191 Synthesis of PC
    R-HSA-1483213 Synthesis of PE

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoethanolamine/phosphocholine phosphatase (EC:3.1.3.75)
    Gene namesi
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 17

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000173868.11
    HGNCiHGNC:16815 PHOSPHO1
    neXtProtiNX_Q8TCT1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi32D → N: Abolishes phosphatase activity. 1 Publication1
    Mutagenesisi43D → N: Strongly reduces reactivity toward PEA and PCho substrates. Abolishes phosphatase activity; when associated with N-123. 1 Publication1
    Mutagenesisi123D → N: Strongly reduces reactivity toward PEA and PCho substrates. Abolishes phosphatase activity; when associated with N-43. 1 Publication1
    Mutagenesisi203D → S: Abolishes phosphatase activity. 1 Publication1

    Organism-specific databases

    DisGeNETi162466
    OpenTargetsiENSG00000173868
    PharmGKBiPA33276

    Chemistry databases

    ChEMBLiCHEMBL6113
    DrugBankiDB00122 Choline

    Polymorphism and mutation databases

    BioMutaiPHOSPHO1
    DMDMi74715842

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000688291 – 267Phosphoethanolamine/phosphocholine phosphataseAdd BLAST267

    Proteomic databases

    PaxDbiQ8TCT1
    PeptideAtlasiQ8TCT1
    PRIDEiQ8TCT1
    ProteomicsDBi74155
    74156 [Q8TCT1-2]

    PTM databases

    DEPODiQ8TCT1
    iPTMnetiQ8TCT1
    PhosphoSitePlusiQ8TCT1

    Expressioni

    Tissue specificityi

    Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.1 Publication

    Gene expression databases

    BgeeiENSG00000173868
    CleanExiHS_PHOSPHO1
    ExpressionAtlasiQ8TCT1 baseline and differential
    GenevisibleiQ8TCT1 HS

    Organism-specific databases

    HPAiHPA053016
    HPA065025

    Interactioni

    Protein-protein interaction databases

    BioGridi127819, 5 interactors
    IntActiQ8TCT1, 51 interactors
    STRINGi9606.ENSP00000406909

    Chemistry databases

    BindingDBiQ8TCT1

    Structurei

    3D structure databases

    ProteinModelPortaliQ8TCT1
    SMRiQ8TCT1
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiKOG3120 Eukaryota
    ENOG4111NAQ LUCA
    GeneTreeiENSGT00390000007741
    HOGENOMiHOG000231038
    HOVERGENiHBG080058
    InParanoidiQ8TCT1
    KOiK06124
    OMAiSPGMGDL
    OrthoDBiEOG091G0GZ1
    PhylomeDBiQ8TCT1
    TreeFamiTF300112

    Family and domain databases

    Gene3Di3.40.50.1000, 1 hit
    InterProiView protein in InterPro
    IPR036412 HAD-like_sf
    IPR023214 HAD_sf
    IPR016965 Pase_PHOSPHO-typ
    IPR006384 PyrdxlP_Pase-rel
    PANTHERiPTHR20889 PTHR20889, 1 hit
    PfamiView protein in Pfam
    PF06888 Put_Phosphatase, 1 hit
    PIRSFiPIRSF031051 PyrdxlP_Pase_PHOSPHO2, 1 hit
    SUPFAMiSSF56784 SSF56784, 1 hit
    TIGRFAMsiTIGR01489 DKMTPPase-SF, 1 hit

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q8TCT1-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MSGCFPVSGL RCLSRDGRMA AQGAPRFLLT FDFDETIVDE NSDDSIVRAA
    60 70 80 90 100
    PGQRLPESLR ATYREGFYNE YMQRVFKYLG EQGVRPRDLS AIYEAIPLSP
    110 120 130 140 150
    GMSDLLQFVA KQGACFEVIL ISDANTFGVE SSLRAAGHHS LFRRILSNPS
    160 170 180 190 200
    GPDARGLLAL RPFHTHSCAR CPANMCKHKV LSDYLRERAH DGVHFERLFY
    210 220 230 240 250
    VGDGANDFCP MGLLAGGDVA FPRRGYPMHR LIQEAQKAEP SSFRASVVPW
    260
    ETAADVRLHL QQVLKSC
    Length:267
    Mass (Da):29,713
    Last modified:June 1, 2002 - v1
    Checksum:i539F65C749D39E81
    GO
    Isoform 2 (identifier: Q8TCT1-2) [UniParc]FASTAAdd to basket
    Also known as: PHOSPHO1-3a

    The sequence of this isoform differs from the canonical sequence as follows:
         1-15: MSGCFPVSGLRCLSR → MCQRLWPANQPLPGGLLPRPLSLAPSSSSSCCSPPCSQ

    Show »
    Length:290
    Mass (Da):32,068
    Checksum:iE7E5C497A540C458
    GO
    Isoform 3 (identifier: Q8TCT1-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-15: MSGCFPVSGLRCLSR → MCQRLWPWPANQPLPGGLLPRPLSLAPSSSSSCCSPPCSQ

    Note: No experimental confirmation available.
    Show »
    Length:292
    Mass (Da):32,351
    Checksum:i8D7E3926D78350B5
    GO

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0406241 – 15MSGCF…RCLSR → MCQRLWPANQPLPGGLLPRP LSLAPSSSSSCCSPPCSQ in isoform 2. 1 PublicationAdd BLAST15
    Alternative sequenceiVSP_0457091 – 15MSGCF…RCLSR → MCQRLWPWPANQPLPGGLLP RPLSLAPSSSSSCCSPPCSQ in isoform 3. 1 PublicationAdd BLAST15

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ457189 mRNA Translation: CAD29803.1
    AC004797 Genomic DNA No translation available.
    BC029931 mRNA No translation available.
    BC117187 mRNA Translation: AAI17188.1
    CCDSiCCDS11547.1 [Q8TCT1-1]
    CCDS45726.1 [Q8TCT1-3]
    RefSeqiNP_001137276.1, NM_001143804.1 [Q8TCT1-3]
    NP_848595.1, NM_178500.3 [Q8TCT1-1]
    UniGeneiHs.405607

    Genome annotation databases

    EnsembliENST00000310544; ENSP00000311925; ENSG00000173868 [Q8TCT1-1]
    ENST00000413580; ENSP00000406909; ENSG00000173868 [Q8TCT1-3]
    ENST00000514112; ENSP00000427694; ENSG00000173868 [Q8TCT1-3]
    GeneIDi162466
    KEGGihsa:162466
    UCSCiuc002ios.2 human [Q8TCT1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiPHOP1_HUMAN
    AccessioniPrimary (citable) accession number: Q8TCT1
    Secondary accession number(s): E9PAM0, Q17RU6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: June 1, 2002
    Last modified: July 18, 2018
    This is version 119 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. SIMILARITY comments
      Index of protein domains and families

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