Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

F-box only protein 25

Gene

FBXO25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • actin binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processUbl conjugation pathway

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
F-box only protein 25
Gene namesi
Name:FBXO25
Synonyms:FBX25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000147364.16
HGNCiHGNC:13596 FBXO25
MIMi609098 gene
neXtProtiNX_Q8TCJ0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi244S → L: Loss of SKP1-binding. 1 Publication1

Organism-specific databases

DisGeNETi26260
OpenTargetsiENSG00000147364
PharmGKBiPA28038

Polymorphism and mutation databases

BioMutaiFBXO25

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001199111 – 367F-box only protein 25Add BLAST367

Proteomic databases

MaxQBiQ8TCJ0
PaxDbiQ8TCJ0
PeptideAtlasiQ8TCJ0
PRIDEiQ8TCJ0
ProteomicsDBi74143
74144 [Q8TCJ0-2]
74145 [Q8TCJ0-3]

PTM databases

iPTMnetiQ8TCJ0
PhosphoSitePlusiQ8TCJ0

Expressioni

Tissue specificityi

Expressed in all brain tissue observed.1 Publication

Gene expression databases

BgeeiENSG00000147364 Expressed in 199 organ(s), highest expression level in testis
CleanExiHS_FBXO25
ExpressionAtlasiQ8TCJ0 baseline and differential
GenevisibleiQ8TCJ0 HS

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO25, SKP1, CUL1 and RBX1. Interacts directly with SKP1 and CUL1. Interacts (via C-terminus) with beta-actin (via N-terminus).2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117646, 194 interactors
IntActiQ8TCJ0, 12 interactors
STRINGi9606.ENSP00000276326

Structurei

3D structure databases

ProteinModelPortaliQ8TCJ0
SMRiQ8TCJ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 274F-boxAdd BLAST49

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 83Interaction with beta-actinAdd BLAST83

Domaini

The F-box is necessary for the interaction with SKP1.By similarity

Phylogenomic databases

eggNOGiKOG3926 Eukaryota
ENOG410YPWK LUCA
GeneTreeiENSGT00390000004915
HOGENOMiHOG000263478
HOVERGENiHBG051572
InParanoidiQ8TCJ0
KOiK10305
OMAiWDIVTLG
OrthoDBiEOG091G0M92
PhylomeDBiQ8TCJ0
TreeFamiTF313070

Family and domain databases

InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
SUPFAMiSSF81383 SSF81383, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8TCJ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MPFLGQDWRS PGWSWIKTED GWKRCESCSQ KLERENNRCN ISHSIILNSE
60 70 80 90 100
DGEIFNNEEH EYASKKRKKD HFRNDTNTQS FYREKWIYVH KESTKERHGY
110 120 130 140 150
CTLGEAFNRL DFSSAIQDIR RFNYVVKLLQ LIAKSQLTSL SGVAQKNYFN
160 170 180 190 200
ILDKIVQKVL DDHHNPRLIK DLLQDLSSTL CILIRGVGKS VLVGNINIWI
210 220 230 240 250
CRLETILAWQ QQLQDLQMTK QVNNGLTLSD LPLHMLNNIL YRFSDGWDII
260 270 280 290 300
TLGQVTPTLY MLSEDRQLWK KLCQYHFAEK QFCRHLILSE KGHIEWKLMY
310 320 330 340 350
FALQKHYPAK EQYGDTLHFC RHCSILFWKD YHLALLFKDS GHPCTAADPD
360
SCFTPVSPQH FIDLFKF
Note: No experimental confirmation available.
Length:367
Mass (Da):43,313
Last modified:March 15, 2005 - v3
Checksum:i1C1C6AD43A92754F
GO
Isoform 2 (identifier: Q8TCJ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     331-339: Missing.

Show »
Length:358
Mass (Da):42,211
Checksum:i11CCEFCA20F3BF37
GO
Isoform 3 (identifier: Q8TCJ0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.
     68-96: KKDHFRNDTNTQSFYREKWIYVHKESTKE → MEKYSIMKSMNMHRKKGKRTILEMTQILK
     331-339: Missing.

Note: No experimental confirmation available.
Show »
Length:291
Mass (Da):34,067
Checksum:i4222DEAEA61A7487
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RFH9E5RFH9_HUMAN
F-box only protein 25
FBXO25
124Annotation score:
H0YBS4H0YBS4_HUMAN
F-box only protein 25
FBXO25
29Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87 – 96IYVHKESTKE → LILTSVLLFQ in AAF04526 (PubMed:10531035).Curated10
Sequence conflicti121R → T in AAF04526 (PubMed:10531035).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04904336N → D. Corresponds to variant dbSNP:rs17665340Ensembl.1
Natural variantiVAR_06116738R → H1 PublicationCorresponds to variant dbSNP:rs10090550Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0130601 – 67Missing in isoform 3. 1 PublicationAdd BLAST67
Alternative sequenceiVSP_01306168 – 96KKDHF…ESTKE → MEKYSIMKSMNMHRKKGKRT ILEMTQILK in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_007374331 – 339Missing in isoform 2 and isoform 3. 4 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB070270 mRNA Translation: BAB85128.1
AF419858 mRNA Translation: AAP97293.1
BC020249 mRNA Translation: AAH20249.1
BC050393 mRNA Translation: AAH50393.1
AF174605 mRNA Translation: AAF04526.1
CCDSiCCDS5952.1 [Q8TCJ0-2]
CCDS5953.1 [Q8TCJ0-1]
CCDS5954.1 [Q8TCJ0-3]
RefSeqiNP_036305.2, NM_012173.3 [Q8TCJ0-3]
NP_904356.1, NM_183420.1 [Q8TCJ0-2]
NP_904357.1, NM_183421.1 [Q8TCJ0-1]
XP_011533050.1, XM_011534748.2 [Q8TCJ0-1]
XP_016868802.1, XM_017013313.1 [Q8TCJ0-1]
UniGeneiHs.438454

Genome annotation databases

EnsembliENST00000276326; ENSP00000276326; ENSG00000147364 [Q8TCJ0-3]
ENST00000350302; ENSP00000342077; ENSG00000147364 [Q8TCJ0-2]
ENST00000352684; ENSP00000341345; ENSG00000147364 [Q8TCJ0-3]
ENST00000382824; ENSP00000372274; ENSG00000147364 [Q8TCJ0-1]
GeneIDi26260
KEGGihsa:26260
UCSCiuc003woy.4 human [Q8TCJ0-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB070270 mRNA Translation: BAB85128.1
AF419858 mRNA Translation: AAP97293.1
BC020249 mRNA Translation: AAH20249.1
BC050393 mRNA Translation: AAH50393.1
AF174605 mRNA Translation: AAF04526.1
CCDSiCCDS5952.1 [Q8TCJ0-2]
CCDS5953.1 [Q8TCJ0-1]
CCDS5954.1 [Q8TCJ0-3]
RefSeqiNP_036305.2, NM_012173.3 [Q8TCJ0-3]
NP_904356.1, NM_183420.1 [Q8TCJ0-2]
NP_904357.1, NM_183421.1 [Q8TCJ0-1]
XP_011533050.1, XM_011534748.2 [Q8TCJ0-1]
XP_016868802.1, XM_017013313.1 [Q8TCJ0-1]
UniGeneiHs.438454

3D structure databases

ProteinModelPortaliQ8TCJ0
SMRiQ8TCJ0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117646, 194 interactors
IntActiQ8TCJ0, 12 interactors
STRINGi9606.ENSP00000276326

PTM databases

iPTMnetiQ8TCJ0
PhosphoSitePlusiQ8TCJ0

Polymorphism and mutation databases

BioMutaiFBXO25

Proteomic databases

MaxQBiQ8TCJ0
PaxDbiQ8TCJ0
PeptideAtlasiQ8TCJ0
PRIDEiQ8TCJ0
ProteomicsDBi74143
74144 [Q8TCJ0-2]
74145 [Q8TCJ0-3]

Protocols and materials databases

DNASUi26260
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276326; ENSP00000276326; ENSG00000147364 [Q8TCJ0-3]
ENST00000350302; ENSP00000342077; ENSG00000147364 [Q8TCJ0-2]
ENST00000352684; ENSP00000341345; ENSG00000147364 [Q8TCJ0-3]
ENST00000382824; ENSP00000372274; ENSG00000147364 [Q8TCJ0-1]
GeneIDi26260
KEGGihsa:26260
UCSCiuc003woy.4 human [Q8TCJ0-1]

Organism-specific databases

CTDi26260
DisGeNETi26260
EuPathDBiHostDB:ENSG00000147364.16
GeneCardsiFBXO25
H-InvDBiHIX0057066
HGNCiHGNC:13596 FBXO25
MIMi609098 gene
neXtProtiNX_Q8TCJ0
OpenTargetsiENSG00000147364
PharmGKBiPA28038
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3926 Eukaryota
ENOG410YPWK LUCA
GeneTreeiENSGT00390000004915
HOGENOMiHOG000263478
HOVERGENiHBG051572
InParanoidiQ8TCJ0
KOiK10305
OMAiWDIVTLG
OrthoDBiEOG091G0M92
PhylomeDBiQ8TCJ0
TreeFamiTF313070

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

ChiTaRSiFBXO25 human
GenomeRNAii26260
PROiPR:Q8TCJ0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000147364 Expressed in 199 organ(s), highest expression level in testis
CleanExiHS_FBXO25
ExpressionAtlasiQ8TCJ0 baseline and differential
GenevisibleiQ8TCJ0 HS

Family and domain databases

InterProiView protein in InterPro
IPR036047 F-box-like_dom_sf
SUPFAMiSSF81383 SSF81383, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFBX25_HUMAN
AccessioniPrimary (citable) accession number: Q8TCJ0
Secondary accession number(s): Q6PJ83, Q7Z4V4, Q9UKB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: March 15, 2005
Last modified: September 12, 2018
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again