UniProtKB - Q8TCG2 (P4K2B_HUMAN)
Protein
Phosphatidylinositol 4-kinase type 2-beta
Gene
PI4K2B
Organism
Homo sapiens (Human)
Status
Functioni
Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking.1 Publication
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H+EC:2.7.1.67
Activity regulationi
Recruited and activated by membrane association. Binding to the microsomal membrane has been shown to enhance its activity.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 148 | ATPBy similarity | 1 | |
Binding sitei | 344 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 127 – 133 | ATPBy similarity | 7 | |
Nucleotide bindingi | 257 – 260 | ATPBy similarity | 4 |
GO - Molecular functioni
- 1-phosphatidylinositol 4-kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- endosome organization Source: GO_Central
- Golgi organization Source: GO_Central
- phosphatidylinositol biosynthetic process Source: Reactome
- phosphatidylinositol phosphorylation Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00529-MONOMER |
BRENDAi | 2.7.1.67, 2681 |
PathwayCommonsi | Q8TCG2 |
Reactomei | R-HSA-1483248, Synthesis of PIPs at the ER membrane R-HSA-1660499, Synthesis of PIPs at the plasma membrane R-HSA-1660514, Synthesis of PIPs at the Golgi membrane R-HSA-1660516, Synthesis of PIPs at the early endosome membrane |
SignaLinki | Q8TCG2 |
SIGNORi | Q8TCG2 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol 4-kinase type 2-beta (EC:2.7.1.67)Alternative name(s): Phosphatidylinositol 4-kinase type II-beta Short name: PI4KII-BETA |
Gene namesi | Name:PI4K2B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18215, PI4K2B |
MIMi | 612101, gene |
neXtProti | NX_Q8TCG2 |
VEuPathDBi | HostDB:ENSG00000038210.12 |
Subcellular locationi
Other locations
Note: Mostly cytoplasmic but also found associated with the plasma membrane, the Golgi and endosomes. Compared to PI4K2A, a larger fraction of PI4K2B is cytosolic due to a smaller extent of palmitoylation. Translocates to membranes where it is recruited by PDGF stimulation by a Rac-GTP-dependent mechanism.
Cytosol
- cytosol Source: Reactome
Endosome
- endosome Source: GO_Central
Golgi apparatus
- trans-Golgi network Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 55300 |
OpenTargetsi | ENSG00000038210 |
PharmGKBi | PA142671173 |
Miscellaneous databases
Pharosi | Q8TCG2, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3220 |
DrugBanki | DB02709, Resveratrol |
DrugCentrali | Q8TCG2 |
GuidetoPHARMACOLOGYi | 2499 |
Genetic variation databases
BioMutai | PI4K2B |
DMDMi | 74715788 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000285164 | 1 – 481 | Phosphatidylinositol 4-kinase type 2-betaAdd BLAST | 481 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 17 | PhosphoserineCombined sources | 1 | |
Modified residuei | 45 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q8TCG2 |
jPOSTi | Q8TCG2 |
MassIVEi | Q8TCG2 |
MaxQBi | Q8TCG2 |
PaxDbi | Q8TCG2 |
PeptideAtlasi | Q8TCG2 |
PRIDEi | Q8TCG2 |
ProteomicsDBi | 74134 |
PTM databases
iPTMneti | Q8TCG2 |
PhosphoSitePlusi | Q8TCG2 |
SwissPalmi | Q8TCG2 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000038210, Expressed in secondary oocyte and 201 other tissues |
ExpressionAtlasi | Q8TCG2, baseline and differential |
Genevisiblei | Q8TCG2, HS |
Organism-specific databases
HPAi | ENSG00000038210, Tissue enhanced (intestine) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 120587, 35 interactors |
IntActi | Q8TCG2, 24 interactors |
STRINGi | 9606.ENSP00000264864 |
Miscellaneous databases
RNActi | Q8TCG2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q8TCG2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 129 – 436 | PI3K/PI4KAdd BLAST | 308 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 153 – 155 | Important for substrate bindingBy similarity | 3 | |
Regioni | 161 – 174 | Important for interaction with membranesBy similarityAdd BLAST | 14 | |
Regioni | 264 – 272 | Important for interaction with membranesBy similarity | 9 | |
Regioni | 357 – 366 | Important for interaction with membranesBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 19 – 22 | Poly-Glu | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2381, Eukaryota |
GeneTreei | ENSGT00390000010434 |
HOGENOMi | CLU_032516_1_0_1 |
InParanoidi | Q8TCG2 |
OMAi | ECGVFPE |
OrthoDBi | 1273723at2759 |
PhylomeDBi | Q8TCG2 |
TreeFami | TF314740 |
Family and domain databases
InterProi | View protein in InterPro IPR039756, Lsb6/PI4K2 IPR000403, PI3/4_kinase_cat_dom |
PANTHERi | PTHR12865, PTHR12865, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8TCG2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEDPSEPDRL ASADGGSPEE EEDGEREPLL PRIAWAHPRR GAPGSAVRLL
60 70 80 90 100
DAAGEEGEAG DEELPLPPGD VGVSRSSSAE LDRSRPAVSV TIGTSEMNAF
110 120 130 140 150
LDDPEFADIM LRAEQAIEVG IFPERISQGS SGSYFVKDPK RKIIGVFKPK
160 170 180 190 200
SEEPYGQLNP KWTKYVHKVC CPCCFGRGCL IPNQGYLSEA GAYLVDNKLH
210 220 230 240 250
LSIVPKTKVV WLVSETFNYN AIDRAKSRGK KYALEKVPKV GRKFHRIGLP
260 270 280 290 300
PKIGSFQLFV EGYKEAEYWL RKFEADPLPE NIRKQFQSQF ERLVILDYII
310 320 330 340 350
RNTDRGNDNW LVRYEKQKCE KEIDHKESKW IDDEEFLIKI AAIDNGLAFP
360 370 380 390 400
FKHPDEWRAY PFHWAWLPQA KVPFSEEIRN LILPYISDMN FVQDLCEDLY
410 420 430 440 450
ELFKTDKGFD KATFESQMSV MRGQILNLTQ ALRDGKSPFQ LVQIPCVIVE
460 470 480
RSQGGSQGRI VHLSNSFTQT VNCRKPFFSS W
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG5E9Z4 | G5E9Z4_HUMAN | Phosphatidylinositol 4-kinase type ... | PI4K2B hCG_19614 | 385 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_031974 | 78 | S → PCombined sources4 PublicationsCorresponds to variant dbSNP:rs313549Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY065990 mRNA Translation: AAL47580.1 AF411320 mRNA Translation: AAL04154.1 AY091514 mRNA Translation: AAM12049.1 AK001967 mRNA Translation: BAA92006.1 BC051749 mRNA Translation: AAH51749.1 |
CCDSi | CCDS3433.1 |
RefSeqi | NP_060793.2, NM_018323.3 |
Genome annotation databases
Ensembli | ENST00000264864; ENSP00000264864; ENSG00000038210 |
GeneIDi | 55300 |
KEGGi | hsa:55300 |
UCSCi | uc003grk.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY065990 mRNA Translation: AAL47580.1 AF411320 mRNA Translation: AAL04154.1 AY091514 mRNA Translation: AAM12049.1 AK001967 mRNA Translation: BAA92006.1 BC051749 mRNA Translation: AAH51749.1 |
CCDSi | CCDS3433.1 |
RefSeqi | NP_060793.2, NM_018323.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4WTV | X-ray | 1.90 | A/B | 90-165 | [»] | |
A/B | 176-450 | [»] | ||||
SMRi | Q8TCG2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120587, 35 interactors |
IntActi | Q8TCG2, 24 interactors |
STRINGi | 9606.ENSP00000264864 |
Chemistry databases
ChEMBLi | CHEMBL3220 |
DrugBanki | DB02709, Resveratrol |
DrugCentrali | Q8TCG2 |
GuidetoPHARMACOLOGYi | 2499 |
PTM databases
iPTMneti | Q8TCG2 |
PhosphoSitePlusi | Q8TCG2 |
SwissPalmi | Q8TCG2 |
Genetic variation databases
BioMutai | PI4K2B |
DMDMi | 74715788 |
Proteomic databases
EPDi | Q8TCG2 |
jPOSTi | Q8TCG2 |
MassIVEi | Q8TCG2 |
MaxQBi | Q8TCG2 |
PaxDbi | Q8TCG2 |
PeptideAtlasi | Q8TCG2 |
PRIDEi | Q8TCG2 |
ProteomicsDBi | 74134 |
Protocols and materials databases
Antibodypediai | 1318, 180 antibodies |
DNASUi | 55300 |
Genome annotation databases
Ensembli | ENST00000264864; ENSP00000264864; ENSG00000038210 |
GeneIDi | 55300 |
KEGGi | hsa:55300 |
UCSCi | uc003grk.3, human |
Organism-specific databases
CTDi | 55300 |
DisGeNETi | 55300 |
GeneCardsi | PI4K2B |
HGNCi | HGNC:18215, PI4K2B |
HPAi | ENSG00000038210, Tissue enhanced (intestine) |
MIMi | 612101, gene |
neXtProti | NX_Q8TCG2 |
OpenTargetsi | ENSG00000038210 |
PharmGKBi | PA142671173 |
VEuPathDBi | HostDB:ENSG00000038210.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2381, Eukaryota |
GeneTreei | ENSGT00390000010434 |
HOGENOMi | CLU_032516_1_0_1 |
InParanoidi | Q8TCG2 |
OMAi | ECGVFPE |
OrthoDBi | 1273723at2759 |
PhylomeDBi | Q8TCG2 |
TreeFami | TF314740 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS00529-MONOMER |
BRENDAi | 2.7.1.67, 2681 |
PathwayCommonsi | Q8TCG2 |
Reactomei | R-HSA-1483248, Synthesis of PIPs at the ER membrane R-HSA-1660499, Synthesis of PIPs at the plasma membrane R-HSA-1660514, Synthesis of PIPs at the Golgi membrane R-HSA-1660516, Synthesis of PIPs at the early endosome membrane |
SignaLinki | Q8TCG2 |
SIGNORi | Q8TCG2 |
Miscellaneous databases
BioGRID-ORCSi | 55300, 7 hits in 875 CRISPR screens |
ChiTaRSi | PI4K2B, human |
GeneWikii | PI4K2B |
GenomeRNAii | 55300 |
Pharosi | Q8TCG2, Tbio |
PROi | PR:Q8TCG2 |
RNActi | Q8TCG2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000038210, Expressed in secondary oocyte and 201 other tissues |
ExpressionAtlasi | Q8TCG2, baseline and differential |
Genevisiblei | Q8TCG2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR039756, Lsb6/PI4K2 IPR000403, PI3/4_kinase_cat_dom |
PANTHERi | PTHR12865, PTHR12865, 1 hit |
Pfami | View protein in Pfam PF00454, PI3_PI4_kinase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | P4K2B_HUMAN | |
Accessioni | Q8TCG2Primary (citable) accession number: Q8TCG2 Secondary accession number(s): Q9NUW2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | June 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families