UBA3

Functionⁱ

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.3 Publications

Miscellaneous

Arg-211 acts as a selectivity gate, preventing misactivation of ubiquitin by this NEDD8-specific E1 complex.

Activity regulationⁱ

Binding of TP53BP2 to the regulatory subunit NAE1 decreases activity.

Pathwayⁱ: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	211	Determines specificity for NEDD8		1
Active siteⁱ	237	Glycyl thioester intermediate		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	100 – 124	ATP1 PublicationAdd BLAST		25
Nucleotide bindingⁱ	148 – 171	ATP1 PublicationAdd BLAST		24

GO - Molecular functionⁱ

acid-amino acid ligase activity Source: InterPro
ATP binding Source: UniProtKB-KW
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 25416956
NEDD8 activating enzyme activity
Source: MGI
Inferred from direct assayⁱ
- 10207026
NEDD8 transferase activity Source: Reactome
nuclear receptor binding Source: Ensembl
protein heterodimerization activity
Source: UniProtKB
Inferred from physical interactionⁱ
- 12740388

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cellular protein modification process
Source: ProtInc
Traceable author statementⁱ
- 9694792
endomitotic cell cycle Source: Ensembl
negative regulation of transcription, DNA-templated Source: Ensembl
post-translational protein modification Source: Reactome
protein neddylation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
proteolysis
Source: ProtInc
Traceable author statementⁱ
- 9694792
regulation of cell cycle Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ligase
Biological process	Cell cycle, Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS07200-MONOMER
BRENDAⁱ	2.3.2.B5 2681 6.2.1.B9 2681
Reactomeⁱ	R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling R-HSA-5676590 NIK-->noncanonical NF-kB signaling R-HSA-8951664 Neddylation R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SIGNORⁱ	Q8TBC4
UniPathwayⁱ	UPA00885

Protein family/group databases

MoonDBⁱ	Q8TBC4 Predicted

MoonDBⁱ

Q8TBC4 Predicted

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: NEDD8-activating enzyme E1 catalytic subunit (EC:6.2.1.-) Alternative name(s): NEDD8-activating enzyme E1C Ubiquitin-activating enzyme E1C Ubiquitin-like modifier-activating enzyme 3 Short name: Ubiquitin-activating enzyme 3
Gene namesⁱ	Name:UBA3 Synonyms:UBE1C
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 3

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000144744.16
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:12470 UBA3
MIMⁱ	603172 gene
neXtProtⁱ	NX_Q8TBC4

Subcellular locationⁱ

GO - Cellular component

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	65	F → G: Reduces affinity for UBE2M. 1 Publication	1
Mutagenesisⁱ	148	I → A: No effect on NEDD8 adenylation. 1 Publication	1
Mutagenesisⁱ	160 – 161	HI → AA: Reduces affinity for UBE2M. 1 Publication	2
Mutagenesisⁱ	167	D → A: Abolishes NEDD8 adenylation. 1 Publication	1
Mutagenesisⁱ	192	P → A: Reduces affinity for UBE2M; when associated with A-195 and A-197. 1 Publication	1
Mutagenesisⁱ	195	I → A: Reduces affinity for UBE2M; when associated with A-192 and A-197. 1 Publication	1
Mutagenesisⁱ	197	P → A: Reduces affinity for UBE2M; when associated with A-192 and A-195. 1 Publication	1
Mutagenesisⁱ	211	R → Q: Abolishes specificity for NEDD8. 1 Publication	1
Mutagenesisⁱ	214	L → A: Reduces affinity for UBE2M; when associated with A-217. 1 Publication	1
Mutagenesisⁱ	217	M → A: Reduces affinity for UBE2M; when associated with A-214. 1 Publication	1
Mutagenesisⁱ	227 – 228	LY → DD: Strongly reduces NEDD8 adenylation. 1 Publication	2
Mutagenesisⁱ	237	C → S: Abolishes thioester intermediate formation. 1 Publication	1
Mutagenesisⁱ	238	T → A: No effect on NEDD8 adenylation; impairs thioester intermediate formation. 1 Publication	1
Mutagenesisⁱ	310	I → A: No effect on NEDD8 adenylation or thioester intermediate formation; impairs NEDD8 transfer to UBE2M. 1 Publication	1
Mutagenesisⁱ	331	I → A: Reduces affinity for UBE2M. 1 Publication	1
Mutagenesisⁱ	352 – 357	YTYTFE → ATATA: Abolishes NEDD8 adenylation. 1 Publication	6
Mutagenesisⁱ	368	S → P: Impairs NEDD8 transfer to UBE2M. 1 Publication	1
Mutagenesisⁱ	369	Q → P: No effect on NEDD8 transfer to UBE2M. 1 Publication	1
Mutagenesisⁱ	370	L → P: Impairs NEDD8 transfer to UBE2M. 1 Publication	1
Mutagenesisⁱ	412	T → A: Impairs NEDD8 transfer to UBE2M. 1 Publication	1
Mutagenesisⁱ	415	L → A: Impairs NEDD8 transfer to UBE2M. 1 Publication	1
Mutagenesisⁱ	418	V → A: Impairs NEDD8 transfer to UBE2M. 1 Publication	1
Mutagenesisⁱ	421	I → A: Impairs NEDD8 transfer to UBE2M. 1 Publication	1
Mutagenesisⁱ	424	R → A: No effect on NEDD8 transfer to UBE2M. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	9039
Open Targets More...OpenTargetsⁱ	ENSG00000144744
PharmGKBⁱ	PA162407622

Chemistry databases

ChEMBLⁱ	CHEMBL2016430

ChEMBLⁱ

CHEMBL2016430

Polymorphism and mutation databases

BioMutaⁱ	UBA3
DMDMⁱ	83305811

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000194941	2 – 463	NEDD8-activating enzyme E1 catalytic subunitAdd BLAST		462

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylalanineCombined sources		1

Keywords - PTMⁱ

Acetylation

Proteomic databases

EPDⁱ	Q8TBC4
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q8TBC4
PaxDbⁱ	Q8TBC4
PeptideAtlas More...PeptideAtlasⁱ	Q8TBC4
PRIDEⁱ	Q8TBC4
ProteomicsDBⁱ	73991 73992 [Q8TBC4-2]

PTM databases

iPTMnetⁱ	Q8TBC4
PhosphoSitePlusⁱ	Q8TBC4

Expressionⁱ

Tissue specificityⁱ

Ubiquitously expressed.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000144744 Expressed in 235 organ(s), highest expression level in substantia nigra
CleanExⁱ	HS_UBA3
ExpressionAtlasⁱ	Q8TBC4 baseline and differential
Genevisibleⁱ	Q8TBC4 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA034873 HPA034874 HPA065335

HPAⁱ

HPA034873
HPA034874
HPA065335

Interactionⁱ

Subunit structureⁱ

Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand (By similarity). Interacts with TBATA (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
itself		3	EBI-717567,EBI-717567
CPNE2	Q96FN4	5	EBI-717567,EBI-7097057
IMPA2	O14732	5	EBI-717567,EBI-725233
RAD23B	P54727	2	EBI-717567,EBI-954531
UBE2M	P61081	2	EBI-717567,EBI-1041660

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 25416956
nuclear receptor binding Source: Ensembl
protein heterodimerization activity
Source: UniProtKB
Inferred from physical interactionⁱ
- 12740388

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	114503, 46 interactors
CORUMⁱ	Q8TBC4
IntActⁱ	Q8TBC4, 16 interactors
Molecular INTeraction database More...MINTⁱ	Q8TBC4
STRINGⁱ	9606.ENSP00000354340

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	31 – 33	Combined sources	3
Turnⁱ	35 – 38	Combined sources	4
Helixⁱ	39 – 46	Combined sources	8
Helixⁱ	63 – 67	Combined sources	5
Beta strandⁱ	72 – 75	Combined sources	4
Helixⁱ	80 – 90	Combined sources	11
Beta strandⁱ	96 – 100	Combined sources	5
Helixⁱ	106 – 108	Combined sources	3
Turnⁱ	109 – 111	Combined sources	3
Helixⁱ	117 – 119	Combined sources	3
Helixⁱ	124 – 135	Combined sources	12
Beta strandⁱ	142 – 146	Combined sources	5
Helixⁱ	148 – 150	Combined sources	3
Helixⁱ	153 – 156	Combined sources	4
Beta strandⁱ	160 – 164	Combined sources	5
Helixⁱ	169 – 181	Combined sources	13
Beta strandⁱ	185 – 188	Combined sources	4
Beta strandⁱ	189 – 191	Combined sources	3
Helixⁱ	192 – 194	Combined sources	3
Beta strandⁱ	198 – 204	Combined sources	7
Beta strandⁱ	207 – 213	Combined sources	7
Turnⁱ	215 – 217	Combined sources	3
Helixⁱ	221 – 227	Combined sources	7
Helixⁱ	236 – 241	Combined sources	6
Helixⁱ	246 – 255	Combined sources	10
Helixⁱ	257 – 260	Combined sources	4
Helixⁱ	275 – 291	Combined sources	17
Helixⁱ	299 – 306	Combined sources	8
Helixⁱ	314 – 333	Combined sources	20
Beta strandⁱ	341 – 346	Combined sources	6
Beta strandⁱ	348 – 350	Combined sources	3
Beta strandⁱ	352 – 356	Combined sources	5
Turnⁱ	365 – 367	Combined sources	3
Beta strandⁱ	372 – 376	Combined sources	5
Beta strandⁱ	377 – 379	Combined sources	3
Helixⁱ	382 – 391	Combined sources	10
Turnⁱ	393 – 395	Combined sources	3
Beta strandⁱ	401 – 406	Combined sources	6
Beta strandⁱ	409 – 414	Combined sources	6
Helixⁱ	419 – 430	Combined sources	12
Beta strandⁱ	431 – 433	Combined sources	3
Helixⁱ	434 – 436	Combined sources	3
Helixⁱ	437 – 440	Combined sources	4
Beta strandⁱ	443 – 447	Combined sources	5
Beta strandⁱ	451 – 453	Combined sources	3
Beta strandⁱ	455 – 460	Combined sources	6

Show more details

3D structure databases

ProteinModelPortalⁱ	Q8TBC4
SMRⁱ	Q8TBC4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q8TBC4

EvolutionaryTraceⁱ

Q8TBC4

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	53 – 70	Interaction with UBE2M N-terminusAdd BLAST	18
Regionⁱ	157 – 161	Interaction with UBE2M N-terminus	5
Regionⁱ	192 – 217	Interaction with UBE2M N-terminusAdd BLAST	26
Regionⁱ	227 – 229	Interaction with NEDD8	3
Regionⁱ	242 – 248	Interaction with NAE11 Publication	7
Regionⁱ	292 – 295	Interaction with NAE11 Publication	4
Regionⁱ	331 – 338	Interaction with UBE2M N-terminus	8
Regionⁱ	352 – 357	Interaction with NEDD8	6
Regionⁱ	368 – 463	Interaction with UBE2M core domainAdd BLAST	96

Sequence similaritiesⁱ

Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG2015 Eukaryota COG0476 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074831
HOGENOMⁱ	HOG000166793
HOVERGENⁱ	HBG082736
InParanoidⁱ	Q8TBC4
KEGG Orthology (KO) More...KOⁱ	K10686
OMAⁱ	TAFPICT
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0B0K
PhylomeDBⁱ	Q8TBC4
TreeFamⁱ	TF300499

Family and domain databases

Gene3Dⁱ	1.10.10.520, 1 hit
InterProⁱ	View protein in InterPro IPR014929 E2_binding IPR000594 ThiF_NAD_FAD-bd IPR023318 Ub_act_enz_dom_a_sf IPR030468 Uba3 IPR035985 Ubiquitin-activating_enz IPR033127 UBQ-activ_enz_E1_Cys_AS
PANTHERⁱ	PTHR10953:SF6 PTHR10953:SF6, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08825 E2_bind, 1 hit PF00899 ThiF, 1 hit
SMARTⁱ	View protein in SMART SM01181 E2_bind, 1 hit
SUPFAMⁱ	SSF69572 SSF69572, 1 hit
PROSITEⁱ	View protein in PROSITE PS00865 UBIQUITIN_ACTIVAT_2, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q8TBC4-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADGEEPEKK RRRIEELLAE KMAVDGGCGD TGDWEGRWNH VKKFLERSGP 
        60         70         80         90        100
FTHPDFEPST ESLQFLLDTC KVLVIGAGGL GCELLKNLAL SGFRQIHVID 
       110        120        130        140        150
MDTIDVSNLN RQFLFRPKDI GRPKAEVAAE FLNDRVPNCN VVPHFNKIQD 
       160        170        180        190        200
FNDTFYRQFH IIVCGLDSII ARRWINGMLI SLLNYEDGVL DPSSIVPLID 
       210        220        230        240        250
GGTEGFKGNA RVILPGMTAC IECTLELYPP QVNFPMCTIA SMPRLPEHCI 
       260        270        280        290        300
EYVRMLQWPK EQPFGEGVPL DGDDPEHIQW IFQKSLERAS QYNIRGVTYR 
       310        320        330        340        350
LTQGVVKRII PAVASTNAVI AAVCATEVFK IATSAYIPLN NYLVFNDVDG 
       360        370        380        390        400
LYTYTFEAER KENCPACSQL PQNIQFSPSA KLQEVLDYLT NSASLQMKSP 
       410        420        430        440        450
AITATLEGKN RTLYLQSVTS IEERTRPNLS KTLKELGLVD GQELAVADVT 
       460 
TPQTVLFKLH FTS

Length:463

Mass (Da):51,852

Last modified:December 6, 2005 - v2

Checksum:ⁱ4C16DC0EEDE31A76

GO

Isoform 2 (identifier: Q8TBC4-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
8-21: Missing.

« Hide

        10         20         30         40         50
MADGEEPMAV DGGCGDTGDW EGRWNHVKKF LERSGPFTHP DFEPSTESLQ 
        60         70         80         90        100
FLLDTCKVLV IGAGGLGCEL LKNLALSGFR QIHVIDMDTI DVSNLNRQFL 
       110        120        130        140        150
FRPKDIGRPK AEVAAEFLND RVPNCNVVPH FNKIQDFNDT FYRQFHIIVC 
       160        170        180        190        200
GLDSIIARRW INGMLISLLN YEDGVLDPSS IVPLIDGGTE GFKGNARVIL 
       210        220        230        240        250
PGMTACIECT LELYPPQVNF PMCTIASMPR LPEHCIEYVR MLQWPKEQPF 
       260        270        280        290        300
GEGVPLDGDD PEHIQWIFQK SLERASQYNI RGVTYRLTQG VVKRIIPAVA 
       310        320        330        340        350
STNAVIAAVC ATEVFKIATS AYIPLNNYLV FNDVDGLYTY TFEAERKENC 
       360        370        380        390        400
PACSQLPQNI QFSPSAKLQE VLDYLTNSAS LQMKSPAITA TLEGKNRTLY 
       410        420        430        440 
LQSVTSIEER TRPNLSKTLK ELGLVDGQEL AVADVTTPQT VLFKLHFTS

Show »

Length:449

Mass (Da):50,072

Checksum:ⁱC624B0B917B2482B

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F8W8D4	F8W8D4_HUMAN	NEDD8-activating enzyme E1 catalyti... NEDD8-activating enzyme E1 catalytic subunit	UBA3	422	Annotation score:
F8WF86	F8WF86_HUMAN	NEDD8-activating enzyme E1 catalyti... NEDD8-activating enzyme E1 catalytic subunit	UBA3	129	Annotation score:
F8WAT6	F8WAT6_HUMAN	NEDD8-activating enzyme E1 catalyti... NEDD8-activating enzyme E1 catalytic subunit	UBA3	64	Annotation score:
A0A0D9SF81	A0A0D9SF81_HUMAN	NEDD8-activating enzyme E1 catalyti... NEDD8-activating enzyme E1 catalytic subunit	UBA3	41	Annotation score:

Sequence cautionⁱ

The sequence AAC27648 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	146	N → Y in CAB55996 (PubMed:11230166).Curated		1
Sequence conflictⁱ	271	D → G in CAB55996 (PubMed:11230166).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_023945	9	K → R2 PublicationsCorresponds to variant dbSNP:rs17852113Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_041127	8 – 21	Missing in isoform 2. 1 PublicationAdd BLAST		14

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL117566 mRNA Translation: CAB55996.2 AK002159 mRNA Translation: BAG51021.1 AK289392 mRNA Translation: BAF82081.1 AC092060 Genomic DNA No translation available. AC109587 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW65470.1 BC022853 mRNA Translation: AAH22853.1 AF046024 mRNA Translation: AAC27648.1 Different initiation. AB012190 mRNA Translation: BAA33144.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS2909.1 [Q8TBC4-1] CCDS2910.1 [Q8TBC4-2]
PIRⁱ	T17306
NCBI Reference Sequences More...RefSeqⁱ	NP_003959.3, NM_003968.3 [Q8TBC4-1] NP_937838.1, NM_198195.1 [Q8TBC4-2]
UniGeneⁱ	Hs.154320

Genome annotation databases

Ensemblⁱ	ENST00000349511; ENSP00000340041; ENSG00000144744 [Q8TBC4-2] ENST00000361055; ENSP00000354340; ENSG00000144744 [Q8TBC4-1]
GeneIDⁱ	9039
KEGGⁱ	hsa:9039
UCSC genome browser More...UCSCⁱ	uc003dno.4 human [Q8TBC4-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Protein	Similar proteins		Species	Score	Length	Source
Q8TBC4	UBA3 isoform 2		PANTR		463	UniRef100_Q8TBC4
Macaca fascicularis brain cDNA clone: QtrA-15145, similar to human ubiquitin-activating enzyme E1C (UBA3 homolog, yeast)(UBE1C), transcript variant 1, mRNA, RefSeq: NM_003968.3		MACFA		463
NEDD8-activating enzyme E1 catalytic subunit isoform 1		MACMU		463
Uncharacterized protein		COLAP		463
Uncharacterized protein		CERAT		463
+6
Q8TBC4-2	UBA3 isoform 1		PANTR		449	UniRef100_A0A2I3SF87
Uncharacterized protein		COLAP		449
Uncharacterized protein		CERAT		449
Uncharacterized protein		RHIRO		449
Uncharacterized protein		PAPAN		449
+3

Protein	Similar proteins		Species	Score	Length	Source
Q8TBC4	NEDD8-activating enzyme E1 catalytic subunit		PONAB		463	UniRef90_Q8TBC4
NEDD8-activating enzyme E1 catalytic subunit		MOUSE		462
NEDD8-activating enzyme E1 catalytic subunit		RAT		462
UBA3 isoform 2		PANTR		463
Macaca fascicularis brain cDNA clone: QtrA-15145, similar to human ubiquitin-activating enzyme E1C (UBA3 homolog, yeast)(UBE1C), transcript variant 1, mRNA, RefSeq: NM_003968.3		MACFA		463
+125
Q8TBC4-2	Uncharacterized protein		CHICK		444	UniRef90_E1BT61
Ubiquitin-like modifier activating enzyme 3, transcript variant X1		COLLI		463
Uncharacterized protein		FICAL		463
NEDD8-activating enzyme E1 catalytic subunit		Patagioenas fasciata monilis		463
NEDD8-activating enzyme E1 catalytic subunit isoform A		ALLMI		463
+211

Protein	Similar proteins		Species	Score	Length	Source
Q8TBC4	NEDD8-activating enzyme E1 catalytic subunit		PONAB		463	UniRef50_Q8TBC4
NEDD8-activating enzyme E1 catalytic subunit		RAT		462
NEDD8-activating enzyme E1 catalytic subunit		MOUSE		462
Nedd8-activating enzyme E1 catalytic subunit		DROME		450
NEDD8-activating enzyme E1 catalytic subunit		DICDI		442
+682
Q8TBC4-2	NEDD8-activating enzyme E1 catalytic subunit		DANRE		462	UniRef50_Q7ZVX6
CLUMA_CG013370, isoform A		Clunio marinus		546
Uncharacterized protein		ANOCA		511
Uncharacterized protein		Micrurus lemniscatus carvalhoi		472
Uncharacterized protein (Fragment)		Micrurus lemniscatus lemniscatus		465
+357

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL117566 mRNA Translation: CAB55996.2 AK002159 mRNA Translation: BAG51021.1 AK289392 mRNA Translation: BAF82081.1 AC092060 Genomic DNA No translation available. AC109587 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW65470.1 BC022853 mRNA Translation: AAH22853.1 AF046024 mRNA Translation: AAC27648.1 Different initiation. AB012190 mRNA Translation: BAA33144.1
CCDSⁱ	CCDS2909.1 [Q8TBC4-1] CCDS2910.1 [Q8TBC4-2]
PIRⁱ	T17306
RefSeqⁱ	NP_003959.3, NM_003968.3 [Q8TBC4-1] NP_937838.1, NM_198195.1 [Q8TBC4-2]
UniGeneⁱ	Hs.154320

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1R4M	X-ray	3.00	B/D/F/H	33-463	[»]
	1R4N	X-ray	3.60	B/D/F/H	33-463	[»]
	1TT5	X-ray	2.60	B/D	33-463	[»]
	1Y8X	X-ray	2.40	B	368-463	[»]
	1YOV	X-ray	2.60	B/D	22-463	[»]
	2LQ7	NMR	-	A	369-463	[»]
	2NVU	X-ray	2.80	B	33-463	[»]
	3DBH	X-ray	2.85	B/D/F/H	33-463	[»]
	3DBL	X-ray	2.90	B/D/F/H	33-463	[»]
	3DBR	X-ray	3.05	B/D/F/H	33-463	[»]
	3FN1	X-ray	2.50	A	368-463	[»]
	3GZN	X-ray	3.00	B/D	1-463	[»]
	5JJM	X-ray	2.15	G/H/I/J	62-69	[»]
ProteinModelPortalⁱ	Q8TBC4
SMRⁱ	Q8TBC4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	114503, 46 interactors
CORUMⁱ	Q8TBC4
IntActⁱ	Q8TBC4, 16 interactors
MINTⁱ	Q8TBC4
STRINGⁱ	9606.ENSP00000354340

Chemistry databases

ChEMBLⁱ	CHEMBL2016430

ChEMBLⁱ

CHEMBL2016430

Protein family/group databases

MoonDBⁱ	Q8TBC4 Predicted

MoonDBⁱ

Q8TBC4 Predicted

PTM databases

iPTMnetⁱ	Q8TBC4
PhosphoSitePlusⁱ	Q8TBC4

Polymorphism and mutation databases

BioMutaⁱ	UBA3
DMDMⁱ	83305811

Proteomic databases

EPDⁱ	Q8TBC4
MaxQBⁱ	Q8TBC4
PaxDbⁱ	Q8TBC4
PeptideAtlasⁱ	Q8TBC4
PRIDEⁱ	Q8TBC4
ProteomicsDBⁱ	73991 73992 [Q8TBC4-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	9039
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000349511; ENSP00000340041; ENSG00000144744 [Q8TBC4-2] ENST00000361055; ENSP00000354340; ENSG00000144744 [Q8TBC4-1]
GeneIDⁱ	9039
KEGGⁱ	hsa:9039
UCSCⁱ	uc003dno.4 human [Q8TBC4-1]

Organism-specific databases

CTDⁱ	9039
DisGeNETⁱ	9039
EuPathDBⁱ	HostDB:ENSG00000144744.16
GeneCardsⁱ	UBA3
HGNCⁱ	HGNC:12470 UBA3
HPAⁱ	HPA034873 HPA034874 HPA065335
MIMⁱ	603172 gene
neXtProtⁱ	NX_Q8TBC4
OpenTargetsⁱ	ENSG00000144744
PharmGKBⁱ	PA162407622
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG2015 Eukaryota COG0476 LUCA
GeneTreeⁱ	ENSGT00550000074831
HOGENOMⁱ	HOG000166793
HOVERGENⁱ	HBG082736
InParanoidⁱ	Q8TBC4
KOⁱ	K10686
OMAⁱ	TAFPICT
OrthoDBⁱ	EOG091G0B0K
PhylomeDBⁱ	Q8TBC4
TreeFamⁱ	TF300499

Enzyme and pathway databases

UniPathwayⁱ	UPA00885
BioCycⁱ	MetaCyc:HS07200-MONOMER
BRENDAⁱ	2.3.2.B5 2681 6.2.1.B9 2681
Reactomeⁱ	R-HSA-5607761 Dectin-1 mediated noncanonical NF-kB signaling R-HSA-5676590 NIK-->noncanonical NF-kB signaling R-HSA-8951664 Neddylation R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SIGNORⁱ	Q8TBC4

Miscellaneous databases

ChiTaRSⁱ	UBA3 human
EvolutionaryTraceⁱ	Q8TBC4
GeneWikiⁱ	UBE1C
GenomeRNAiⁱ	9039
Protein Ontology More...PROⁱ	PR:Q8TBC4
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000144744 Expressed in 235 organ(s), highest expression level in substantia nigra
CleanExⁱ	HS_UBA3
ExpressionAtlasⁱ	Q8TBC4 baseline and differential
Genevisibleⁱ	Q8TBC4 HS

Family and domain databases

Gene3Dⁱ	1.10.10.520, 1 hit
InterProⁱ	View protein in InterPro IPR014929 E2_binding IPR000594 ThiF_NAD_FAD-bd IPR023318 Ub_act_enz_dom_a_sf IPR030468 Uba3 IPR035985 Ubiquitin-activating_enz IPR033127 UBQ-activ_enz_E1_Cys_AS
PANTHERⁱ	PTHR10953:SF6 PTHR10953:SF6, 1 hit
Pfamⁱ	View protein in Pfam PF08825 E2_bind, 1 hit PF00899 ThiF, 1 hit
SMARTⁱ	View protein in SMART SM01181 E2_bind, 1 hit
SUPFAMⁱ	SSF69572 SSF69572, 1 hit
PROSITEⁱ	View protein in PROSITE PS00865 UBIQUITIN_ACTIVAT_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBA3_HUMAN
Accessionⁱ	Q8TBC4Primary (citable) accession number: Q8TBC4 Secondary accession number(s): A6NLB5 , A8K027, O76088, Q9NTU3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
	Last sequence update:	December 6, 2005
	Last modified:	November 7, 2018
	This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - Q8TBC4 (UBA3_HUMAN)

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NEDD8-activating enzyme E1 catalytic subunit

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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Keywords - PTMi

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Gene expression databases

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<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

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Keywords - Coding sequence diversityi

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<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

Pathwayⁱ: protein neddylation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ