Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
The new UniProt website is here! Take me to UniProt BETA

UniProtKB - Q8TBC4 (UBA3_HUMAN)

Entry version 192 (25 May 2022)
Sequence version 2 (06 Dec 2005)
Previous versions | rss
Add a publicationFeedback
Protein

NEDD8-activating enzyme E1 catalytic subunit

Gene

UBA3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

3 Publications

Miscellaneous

Arg-211 acts as a selectivity gate, preventing misactivation of ubiquitin by this NEDD8-specific E1 complex.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Binding of TP53BP2 to the regulatory subunit NAE1 decreases activity.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei211Determines specificity for NEDD81
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei237Glycyl thioester intermediate1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi100 – 124ATP1 PublicationAdd BLAST25
Nucleotide bindingi148 – 171ATP1 PublicationAdd BLAST24

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigase
Biological processCell cycle, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.3.2.23, 2681
6.2.1.64, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		Q8TBC4

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-5607761, Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5676590, NIK-->noncanonical NF-kB signaling
R-HSA-8951664, Neddylation
R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		Q8TBC4

SIGNOR Signaling Network Open Resource

More...SIGNORi		Q8TBC4

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00885

Protein family/group databases

MoonDB Database of extreme multifunctional and moonlighting proteins

More...MoonDBi		Q8TBC4, Predicted

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
NEDD8-activating enzyme E1 catalytic subunit (EC:6.2.1.641 Publication)
Alternative name(s):
NEDD8-activating enzyme E1C
Ubiquitin-activating enzyme E1C
Ubiquitin-like modifier-activating enzyme 3
Short name:
Ubiquitin-activating enzyme 3
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:UBA3
Synonyms:UBE1C
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:12470, UBA3

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		603172, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_Q8TBC4

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000144744

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi65F → G: Reduces affinity for UBE2M. 1 Publication1
Mutagenesisi148I → A: No effect on NEDD8 adenylation. 1 Publication1
Mutagenesisi160 – 161HI → AA: Reduces affinity for UBE2M. 1 Publication2
Mutagenesisi167D → A: Abolishes NEDD8 adenylation. 1 Publication1
Mutagenesisi192P → A: Reduces affinity for UBE2M; when associated with A-195 and A-197. 1 Publication1
Mutagenesisi195I → A: Reduces affinity for UBE2M; when associated with A-192 and A-197. 1 Publication1
Mutagenesisi197P → A: Reduces affinity for UBE2M; when associated with A-192 and A-195. 1 Publication1
Mutagenesisi211R → Q: Abolishes specificity for NEDD8. 1 Publication1
Mutagenesisi214L → A: Reduces affinity for UBE2M; when associated with A-217. 1 Publication1
Mutagenesisi217M → A: Reduces affinity for UBE2M; when associated with A-214. 1 Publication1
Mutagenesisi227 – 228LY → DD: Strongly reduces NEDD8 adenylation. 1 Publication2
Mutagenesisi237C → S: Abolishes thioester intermediate formation. 1 Publication1
Mutagenesisi238T → A: No effect on NEDD8 adenylation; impairs thioester intermediate formation. 1 Publication1
Mutagenesisi310I → A: No effect on NEDD8 adenylation or thioester intermediate formation; impairs NEDD8 transfer to UBE2M. 1 Publication1
Mutagenesisi331I → A: Reduces affinity for UBE2M. 1 Publication1
Mutagenesisi352 – 357YTYTFE → ATATA: Abolishes NEDD8 adenylation. 1 Publication6
Mutagenesisi368S → P: Impairs NEDD8 transfer to UBE2M. 1 Publication1
Mutagenesisi369Q → P: No effect on NEDD8 transfer to UBE2M. 1 Publication1
Mutagenesisi370L → P: Impairs NEDD8 transfer to UBE2M. 1 Publication1
Mutagenesisi412T → A: Impairs NEDD8 transfer to UBE2M. 1 Publication1
Mutagenesisi415L → A: Impairs NEDD8 transfer to UBE2M. 1 Publication1
Mutagenesisi418V → A: Impairs NEDD8 transfer to UBE2M. 1 Publication1
Mutagenesisi421I → A: Impairs NEDD8 transfer to UBE2M. 1 Publication1
Mutagenesisi424R → A: No effect on NEDD8 transfer to UBE2M. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		9039

Open Targets

More...OpenTargetsi		ENSG00000144744

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA162407622

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		Q8TBC4, Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL2016430

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		UBA3

Domain mapping of disease mutations (DMDM)

More...DMDMi		83305811

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001949412 – 463NEDD8-activating enzyme E1 catalytic subunitAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q8TBC4

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q8TBC4

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		Q8TBC4

MaxQB - The MaxQuant DataBase

More...MaxQBi		Q8TBC4

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q8TBC4

PeptideAtlas

More...PeptideAtlasi		Q8TBC4

PRoteomics IDEntifications database

More...PRIDEi		Q8TBC4

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		73991 [Q8TBC4-1]
73992 [Q8TBC4-2]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		Q8TBC4, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q8TBC4

MetOSite database of methionine sulfoxide sites

More...MetOSitei		Q8TBC4

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q8TBC4

SwissPalm database of S-palmitoylation events

More...SwissPalmi		Q8TBC4

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000144744, Expressed in substantia nigra and 249 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		Q8TBC4, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q8TBC4, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000144744, Tissue enriched (brain)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of UBA3 and NAE1.

Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand (By similarity).

Interacts with TBATA (By similarity).

By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		114503, 76 interactors

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		Q8TBC4

Protein interaction database and analysis system

More...IntActi		Q8TBC4, 23 interactors

Molecular INTeraction database

More...MINTi		Q8TBC4

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000354340

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		Q8TBC4

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q8TBC4, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1463
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		Q8TBC4

Biological Magnetic Resonance Data Bank

More...BMRBi		Q8TBC4

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q8TBC4

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		Q8TBC4

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni53 – 70Interaction with UBE2M N-terminusAdd BLAST18
Regioni157 – 161Interaction with UBE2M N-terminus5
Regioni192 – 217Interaction with UBE2M N-terminusAdd BLAST26
Regioni227 – 229Interaction with NEDD83
Regioni242 – 248Interaction with NAE11 Publication7
Regioni292 – 295Interaction with NAE11 Publication4
Regioni331 – 338Interaction with UBE2M N-terminus8
Regioni352 – 357Interaction with NEDD86
Regioni368 – 463Interaction with UBE2M core domainAdd BLAST96

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG2015, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00550000074831

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_013325_13_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q8TBC4

Identification of Orthologs from Complete Genome Data

More...OMAi		LENYMMY

Database of Orthologous Groups

More...OrthoDBi		686413at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q8TBC4

TreeFam database of animal gene trees

More...TreeFami		TF300499

Family and domain databases

Database of protein disorder

More...DisProti		DP02306

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.10.520, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR014929, E2-binding
IPR045886, ThiF/MoeB/HesA
IPR000594, ThiF_NAD_FAD-bd
IPR023318, Ub_act_enz_dom_a_sf
IPR030468, Uba3
IPR035985, Ubiquitin-activating_enz
IPR033127, UBQ-activ_enz_E1_Cys_AS

The PANTHER Classification System

More...PANTHERi		PTHR10953, PTHR10953, 1 hit
PTHR10953:SF6, PTHR10953:SF6, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF08825, E2_bind, 1 hit
PF00899, ThiF, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM01181, E2_bind, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF69572, SSF69572, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00865, UBIQUITIN_ACTIVAT_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q8TBC4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MADGEEPEKK RRRIEELLAE KMAVDGGCGD TGDWEGRWNH VKKFLERSGP 
        60         70         80         90        100
FTHPDFEPST ESLQFLLDTC KVLVIGAGGL GCELLKNLAL SGFRQIHVID 
       110        120        130        140        150
MDTIDVSNLN RQFLFRPKDI GRPKAEVAAE FLNDRVPNCN VVPHFNKIQD 
       160        170        180        190        200
FNDTFYRQFH IIVCGLDSII ARRWINGMLI SLLNYEDGVL DPSSIVPLID 
       210        220        230        240        250
GGTEGFKGNA RVILPGMTAC IECTLELYPP QVNFPMCTIA SMPRLPEHCI 
       260        270        280        290        300
EYVRMLQWPK EQPFGEGVPL DGDDPEHIQW IFQKSLERAS QYNIRGVTYR 
       310        320        330        340        350
LTQGVVKRII PAVASTNAVI AAVCATEVFK IATSAYIPLN NYLVFNDVDG 
       360        370        380        390        400
LYTYTFEAER KENCPACSQL PQNIQFSPSA KLQEVLDYLT NSASLQMKSP 
       410        420        430        440        450
AITATLEGKN RTLYLQSVTS IEERTRPNLS KTLKELGLVD GQELAVADVT 
       460 
TPQTVLFKLH FTS
Length:463
Mass (Da):51,852
Last modified:December 6, 2005 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4C16DC0EEDE31A76
GO
Isoform 2 (identifier: Q8TBC4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     8-21: Missing.

Show »
Length:449
Mass (Da):50,072
Checksum:iC624B0B917B2482B
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8W8D4F8W8D4_HUMAN
NEDD8-activating enzyme E1 catalyti...
UBA3
422Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F8WF86F8WF86_HUMAN
NEDD8-activating enzyme E1 catalyti...
UBA3
129Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F8WAT6F8WAT6_HUMAN
NEDD8-activating enzyme E1 catalyti...
UBA3
64Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0D9SF81A0A0D9SF81_HUMAN
NEDD8-activating enzyme E1 catalyti...
UBA3
41Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAC27648 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti146N → Y in CAB55996 (PubMed:11230166).Curated1
Sequence conflicti271D → G in CAB55996 (PubMed:11230166).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_0239459K → R2 PublicationsCorresponds to variant dbSNP:rs17852113Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_0411278 – 21Missing in isoform 2. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AL117566 mRNA Translation: CAB55996.2
AK002159 mRNA Translation: BAG51021.1
AK289392 mRNA Translation: BAF82081.1
AC092060 Genomic DNA No translation available.
AC109587 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW65470.1
BC022853 mRNA Translation: AAH22853.1
AF046024 mRNA Translation: AAC27648.1 Different initiation.
AB012190 mRNA Translation: BAA33144.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS2909.1 [Q8TBC4-1]
CCDS2910.1 [Q8TBC4-2]

Protein sequence database of the Protein Information Resource

More...PIRi		T17306

NCBI Reference Sequences

More...RefSeqi		NP_003959.3, NM_003968.3 [Q8TBC4-1]
NP_937838.1, NM_198195.1 [Q8TBC4-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000349511.8; ENSP00000340041.4; ENSG00000144744.17 [Q8TBC4-2]
ENST00000361055.9; ENSP00000354340.4; ENSG00000144744.17

Database of genes from NCBI RefSeq genomes

More...GeneIDi		9039

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:9039

Matched Annotation from NCBI and EMBL-EBI (MANE) - Phase one

More...MANE-Selecti		ENST00000361055.9; ENSP00000354340.4; NM_003968.4; NP_003959.3

UCSC genome browser

More...UCSCi		uc003dno.4, human [Q8TBC4-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL117566 mRNA Translation: CAB55996.2
AK002159 mRNA Translation: BAG51021.1
AK289392 mRNA Translation: BAF82081.1
AC092060 Genomic DNA No translation available.
AC109587 Genomic DNA No translation available.
CH471055 Genomic DNA Translation: EAW65470.1
BC022853 mRNA Translation: AAH22853.1
AF046024 mRNA Translation: AAC27648.1 Different initiation.
AB012190 mRNA Translation: BAA33144.1
CCDSiCCDS2909.1 [Q8TBC4-1]
CCDS2910.1 [Q8TBC4-2]
PIRiT17306
RefSeqiNP_003959.3, NM_003968.3 [Q8TBC4-1]
NP_937838.1, NM_198195.1 [Q8TBC4-2]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R4MX-ray3.00B/D/F/H33-463[»]
1R4NX-ray3.60B/D/F/H33-463[»]
1TT5X-ray2.60B/D33-463[»]
1Y8XX-ray2.40B368-463[»]
1YOVX-ray2.60B/D22-463[»]
2LQ7NMR-A369-463[»]
2NVUX-ray2.80B33-463[»]
3DBHX-ray2.85B/D/F/H33-463[»]
3DBLX-ray2.90B/D/F/H33-463[»]
3DBRX-ray3.05B/D/F/H33-463[»]
3FN1X-ray2.50A368-463[»]
3GZNX-ray3.00B/D1-463[»]
5JJMX-ray2.15G/H/I/J62-69[»]
AlphaFoldDBiQ8TBC4
BMRBiQ8TBC4
SMRiQ8TBC4
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi114503, 76 interactors
CORUMiQ8TBC4
IntActiQ8TBC4, 23 interactors
MINTiQ8TBC4
STRINGi9606.ENSP00000354340

Chemistry databases

BindingDBiQ8TBC4
ChEMBLiCHEMBL2016430

Protein family/group databases

MoonDBiQ8TBC4, Predicted

PTM databases

GlyGeniQ8TBC4, 1 site, 1 O-linked glycan (1 site)
iPTMnetiQ8TBC4
MetOSiteiQ8TBC4
PhosphoSitePlusiQ8TBC4
SwissPalmiQ8TBC4

Genetic variation databases

BioMutaiUBA3
DMDMi83305811

Proteomic databases

EPDiQ8TBC4
jPOSTiQ8TBC4
MassIVEiQ8TBC4
MaxQBiQ8TBC4
PaxDbiQ8TBC4
PeptideAtlasiQ8TBC4
PRIDEiQ8TBC4
ProteomicsDBi73991 [Q8TBC4-1]
73992 [Q8TBC4-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		31863, 176 antibodies from 29 providers

The DNASU plasmid repository

More...DNASUi		9039

Genome annotation databases

EnsembliENST00000349511.8; ENSP00000340041.4; ENSG00000144744.17 [Q8TBC4-2]
ENST00000361055.9; ENSP00000354340.4; ENSG00000144744.17
GeneIDi9039
KEGGihsa:9039
MANE-SelectiENST00000361055.9; ENSP00000354340.4; NM_003968.4; NP_003959.3
UCSCiuc003dno.4, human [Q8TBC4-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		9039
DisGeNETi9039

GeneCards: human genes, protein and diseases

More...GeneCardsi		UBA3
HGNCiHGNC:12470, UBA3
HPAiENSG00000144744, Tissue enriched (brain)
MIMi603172, gene
neXtProtiNX_Q8TBC4
OpenTargetsiENSG00000144744
PharmGKBiPA162407622
VEuPathDBiHostDB:ENSG00000144744

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG2015, Eukaryota
GeneTreeiENSGT00550000074831
HOGENOMiCLU_013325_13_1_1
InParanoidiQ8TBC4
OMAiLENYMMY
OrthoDBi686413at2759
PhylomeDBiQ8TBC4
TreeFamiTF300499

Enzyme and pathway databases

UniPathwayiUPA00885
BRENDAi2.3.2.23, 2681
6.2.1.64, 2681
PathwayCommonsiQ8TBC4
ReactomeiR-HSA-5607761, Dectin-1 mediated noncanonical NF-kB signaling
R-HSA-5676590, NIK-->noncanonical NF-kB signaling
R-HSA-8951664, Neddylation
R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ8TBC4
SIGNORiQ8TBC4

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		9039, 571 hits in 1107 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		UBA3, human
EvolutionaryTraceiQ8TBC4

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		UBE1C

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		9039
PharosiQ8TBC4, Tchem

Protein Ontology

More...PROi		PR:Q8TBC4
RNActiQ8TBC4, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000144744, Expressed in substantia nigra and 249 other tissues
ExpressionAtlasiQ8TBC4, baseline and differential
GenevisibleiQ8TBC4, HS

Family and domain databases

DisProtiDP02306
Gene3Di1.10.10.520, 1 hit
InterProiView protein in InterPro
IPR014929, E2-binding
IPR045886, ThiF/MoeB/HesA
IPR000594, ThiF_NAD_FAD-bd
IPR023318, Ub_act_enz_dom_a_sf
IPR030468, Uba3
IPR035985, Ubiquitin-activating_enz
IPR033127, UBQ-activ_enz_E1_Cys_AS
PANTHERiPTHR10953, PTHR10953, 1 hit
PTHR10953:SF6, PTHR10953:SF6, 1 hit
PfamiView protein in Pfam
PF08825, E2_bind, 1 hit
PF00899, ThiF, 1 hit
SMARTiView protein in SMART
SM01181, E2_bind, 1 hit
SUPFAMiSSF69572, SSF69572, 1 hit
PROSITEiView protein in PROSITE
PS00865, UBIQUITIN_ACTIVAT_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiUBA3_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q8TBC4
Secondary accession number(s): A6NLB5
, A8K027, O76088, Q9NTU3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: December 6, 2005
Last modified: May 25, 2022
This is version 192 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again