UniProtKB - Q8TB24 (RIN3_HUMAN)
Protein
Ras and Rab interactor 3
Gene
RIN3
Organism
Homo sapiens (Human)
Status
Functioni
Ras effector protein that functions as a guanine nucleotide exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free GTP. Required for normal RAB31 function.2 Publications
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB-KW
- Rab GTPase binding Source: UniProtKB
- Rab guanyl-nucleotide exchange factor activity Source: UniProtKB
GO - Biological processi
- endocytosis Source: UniProtKB
- signal transduction Source: InterPro
Keywordsi
Molecular function | GTPase activation |
Enzyme and pathway databases
PathwayCommonsi | Q8TB24 |
Reactomei | R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
Protein family/group databases
MoonDBi | Q8TB24, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Ras and Rab interactor 3Alternative name(s): Ras interaction/interference protein 3 |
Gene namesi | Name:RIN3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000100599.15 |
HGNCi | HGNC:18751, RIN3 |
MIMi | 610223, gene |
neXtProti | NX_Q8TB24 |
Subcellular locationi
Endosome
- Early endosome 1 Publication
Other locations
- Cytoplasm 2 Publications
- Cytoplasmic vesicle 3 Publications
Note: Activation of tyrosine phosphorylation signaling induces translocation to cytoplasmic vesicles.1 Publication
Cytosol
- cytosol Source: Reactome
Endosome
- early endosome Source: UniProtKB
Other locations
- cytoplasmic vesicle Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, EndosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 825 | Y → A: Strongly reduced guanine nucleotide exchange factor activity toward RAB31; when associated with A-828. 1 Publication | 1 | |
Mutagenesisi | 828 | T → A: Strongly reduced guanine nucleotide exchange factor activity toward RAB31; when associated with A-825. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 79890 |
NIAGADSi | ENSG00000100599 |
OpenTargetsi | ENSG00000100599 |
PharmGKBi | PA38673 |
Miscellaneous databases
Pharosi | Q8TB24, Tbio |
Polymorphism and mutation databases
BioMutai | RIN3 |
DMDMi | 209572780 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191322 | 1 – 985 | Ras and Rab interactor 3Add BLAST | 985 |
Proteomic databases
EPDi | Q8TB24 |
jPOSTi | Q8TB24 |
MassIVEi | Q8TB24 |
PaxDbi | Q8TB24 |
PeptideAtlasi | Q8TB24 |
PRIDEi | Q8TB24 |
ProteomicsDBi | 73951 [Q8TB24-1] 73952 [Q8TB24-4] |
PTM databases
iPTMneti | Q8TB24 |
PhosphoSitePlusi | Q8TB24 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000100599, Expressed in blood and 183 other tissues |
ExpressionAtlasi | Q8TB24, baseline and differential |
Genevisiblei | Q8TB24, HS |
Organism-specific databases
HPAi | ENSG00000100599, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with CD2AP, RAB5B, RAB31 and BIN1.
3 PublicationsBinary interactionsi
Hide detailsQ8TB24
With | #Exp. | IntAct |
---|---|---|
CRK [P46108] | 2 | EBI-1570523,EBI-886 |
NCK1 [P16333] | 2 | EBI-1570523,EBI-389883 |
PLCG1 [P19174] | 3 | EBI-1570523,EBI-79387 |
GO - Molecular functioni
- Rab GTPase binding Source: UniProtKB
- Rab guanyl-nucleotide exchange factor activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 122974, 41 interactors |
IntActi | Q8TB24, 46 interactors |
MINTi | Q8TB24 |
STRINGi | 9606.ENSP00000216487 |
Miscellaneous databases
RNActi | Q8TB24, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 63 – 158 | SH2PROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 703 – 846 | VPS9PROSITE-ProRule annotationAdd BLAST | 144 | |
Domaini | 877 – 963 | Ras-associatingPROSITE-ProRule annotationAdd BLAST | 87 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 587 – 732 | Interaction with RAB5B1 PublicationAdd BLAST | 146 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 24 – 29 | Poly-Glu | 6 | |
Compositional biasi | 248 – 514 | Pro-richAdd BLAST | 267 | |
Compositional biasi | 722 – 725 | Poly-Thr | 4 | |
Compositional biasi | 965 – 972 | Poly-Gly | 8 |
Sequence similaritiesi
Belongs to the RIN (Ras interaction/interference) family.Curated
Keywords - Domaini
SH2 domainPhylogenomic databases
eggNOGi | KOG2320, Eukaryota |
GeneTreei | ENSGT00940000158622 |
InParanoidi | Q8TB24 |
OMAi | QEIRMMM |
PhylomeDBi | Q8TB24 |
TreeFami | TF331067 |
Family and domain databases
CDDi | cd10395, SH2_RIN3, 1 hit |
Gene3Di | 1.20.1050.80, 1 hit 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR000159, RA_dom IPR035869, RIN3_SH2 IPR000980, SH2 IPR036860, SH2_dom_sf IPR003123, VPS9 IPR037191, VPS9_dom_sf |
Pfami | View protein in Pfam PF02204, VPS9, 1 hit |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00167, VPS9, 1 hit |
SUPFAMi | SSF109993, SSF109993, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50200, RA, 1 hit PS50001, SH2, 1 hit PS51205, VPS9, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8TB24-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIRHAGAPAR GDPTGPVPVV GKGEEEEEED GMRLCLPANP KNCLPHRRGI
60 70 80 90 100
SILEKLIKTC PVWLQLSLGQ AEVARILHRV VAGMFLVRRD SSSKQLVLCV
110 120 130 140 150
HFPSLNESSA EVLEYTIKEE KSILYLEGSA LVFEDIFRLI AFYCVSRDLL
160 170 180 190 200
PFTLRLPQAI LEASSFTDLE TIANLGLGFW DSSLNPPQER GKPAEPPRDR
210 220 230 240 250
APGFPLVSSL RPTAHDANCA CEIELSVGND RLWFVNPIFI EDCSSALPTD
260 270 280 290 300
QPPLGNCPAR PLPPTSDATS PTSRWAPRRP PPPPPVLPLQ PCSPAQPPVL
310 320 330 340 350
PALAPAPACP LPTSPPVPAP HVTPHAPGPP DHPNQPPMMT CERLPCPTAG
360 370 380 390 400
LGPLREEAMK PGAASSPLQQ VPAPPLPAKK NLPTAPPRRR VSERVSLEDQ
410 420 430 440 450
SPGMAAEGDQ LSLPPQGTSD GPEDTPREST EQGQDTEVKA SDPHSMPELP
460 470 480 490 500
RTAKQPPVPP PRKKRISRQL ASTLPAPLEN AELCTQAMAL ETPTPGPPRE
510 520 530 540 550
GQSPASQAGT QHPPAQATAH SQSSPEFKGS LASLSDSLGV SVMATDQDSY
560 570 580 590 600
STSSTEEELE QFSSPSVKKK PSMILGKARH RLSFASFSSM FHAFLSNNRK
610 620 630 640 650
LYKKVVELAQ DKGSYFGSLV QDYKVYSLEM MARQTSSTEM LQEIRTMMTQ
660 670 680 690 700
LKSYLLQSTE LKALVDPALH SEEELEAIVE SALYKCVLKP LKEAINSCLH
710 720 730 740 750
QIHSKDGSLQ QLKENQLVIL ATTTTDLGVT TSVPEVPMME KILQKFTSMH
760 770 780 790 800
KAYSPEKKIS ILLKTCKLIY DSMALGNPGK PYGADDFLPV LMYVLARSNL
810 820 830 840 850
TEMLLNVEYM MELMDPALQL GEGSYYLTTT YGALEHIKSY DKITVTRQLS
860 870 880 890 900
VEVQDSIHRW ERRRTLNKAR ASRSSVQDFI CVSYLEPEQQ ARTLASRADT
910 920 930 940 950
QAQALCAQCA EKFAVERPQA HRLFVLVDGR CFQLADDALP HCIKGYLLRS
960 970 980
EPKRDFHFVY RPLDGGGGGG GGSPPCLVVR EPNFL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WWY9 | A0A087WWY9_HUMAN | Ras and Rab interactor 3 | RIN3 | 913 | Annotation score: | ||
H0YJ83 | H0YJ83_HUMAN | Ras and Rab interactor 3 | RIN3 | 420 | Annotation score: | ||
G3V534 | G3V534_HUMAN | Ras and Rab interactor 3 | RIN3 | 78 | Annotation score: | ||
G3V2I7 | G3V2I7_HUMAN | Ras and Rab interactor 3 | RIN3 hCG_2029104 | 159 | Annotation score: |
Sequence cautioni
The sequence BAB15357 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 742 | I → F in AAH25248 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 965 | Missing in BAB15357 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 965 | Missing in AAH25248 (PubMed:15489334).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_046645 | 111 | E → K. Corresponds to variant dbSNP:rs2274542Ensembl. | 1 | |
Natural variantiVAR_059960 | 215 | H → L. Corresponds to variant dbSNP:rs3829947Ensembl. | 1 | |
Natural variantiVAR_046646 | 215 | H → P. Corresponds to variant dbSNP:rs3829947Ensembl. | 1 | |
Natural variantiVAR_059961 | 215 | H → R. Corresponds to variant dbSNP:rs3829947Ensembl. | 1 | |
Natural variantiVAR_052946 | 425 | T → I. Corresponds to variant dbSNP:rs3742717Ensembl. | 1 | |
Natural variantiVAR_046647 | 425 | T → M1 PublicationCorresponds to variant dbSNP:rs3742717Ensembl. | 1 | |
Natural variantiVAR_046648 | 613 | G → A. Corresponds to variant dbSNP:rs12434929Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007587 | 878 – 903 | DFICV…DTQAQ → VRPESGRGPVGPCPRHHPCC LLAKEP in isoform 4. 1 PublicationAdd BLAST | 26 | |
Alternative sequenceiVSP_007588 | 904 – 985 | Missing in isoform 4. 1 PublicationAdd BLAST | 82 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB081753 mRNA Translation: BAC16513.1 AL136332 Genomic DNA No translation available. AL159141 Genomic DNA No translation available. AK021762 mRNA Translation: BAB13888.1 Sequence problems. AK026092 mRNA Translation: BAB15357.1 Different initiation. AK074176 mRNA Translation: BAB85002.1 AK090451 mRNA Translation: BAC03432.1 Sequence problems. BC025248 mRNA Translation: AAH25248.2 AB060338 mRNA Translation: BAB84316.1 |
CCDSi | CCDS32144.1 [Q8TB24-1] |
PIRi | T50623 |
RefSeqi | NP_001306916.1, NM_001319987.1 NP_079108.3, NM_024832.4 [Q8TB24-1] |
Genome annotation databases
Ensembli | ENST00000216487; ENSP00000216487; ENSG00000100599 [Q8TB24-1] |
GeneIDi | 79890 |
KEGGi | hsa:79890 |
UCSCi | uc001yap.4, human [Q8TB24-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB081753 mRNA Translation: BAC16513.1 AL136332 Genomic DNA No translation available. AL159141 Genomic DNA No translation available. AK021762 mRNA Translation: BAB13888.1 Sequence problems. AK026092 mRNA Translation: BAB15357.1 Different initiation. AK074176 mRNA Translation: BAB85002.1 AK090451 mRNA Translation: BAC03432.1 Sequence problems. BC025248 mRNA Translation: AAH25248.2 AB060338 mRNA Translation: BAB84316.1 |
CCDSi | CCDS32144.1 [Q8TB24-1] |
PIRi | T50623 |
RefSeqi | NP_001306916.1, NM_001319987.1 NP_079108.3, NM_024832.4 [Q8TB24-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3U23 | X-ray | 1.11 | B | 452-467 | [»] | |
4WCI | X-ray | 1.65 | B/D/F | 378-393 | [»] | |
SMRi | Q8TB24 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 122974, 41 interactors |
IntActi | Q8TB24, 46 interactors |
MINTi | Q8TB24 |
STRINGi | 9606.ENSP00000216487 |
Protein family/group databases
MoonDBi | Q8TB24, Predicted |
PTM databases
iPTMneti | Q8TB24 |
PhosphoSitePlusi | Q8TB24 |
Polymorphism and mutation databases
BioMutai | RIN3 |
DMDMi | 209572780 |
Proteomic databases
EPDi | Q8TB24 |
jPOSTi | Q8TB24 |
MassIVEi | Q8TB24 |
PaxDbi | Q8TB24 |
PeptideAtlasi | Q8TB24 |
PRIDEi | Q8TB24 |
ProteomicsDBi | 73951 [Q8TB24-1] 73952 [Q8TB24-4] |
Protocols and materials databases
Antibodypediai | 26773, 73 antibodies |
Genome annotation databases
Ensembli | ENST00000216487; ENSP00000216487; ENSG00000100599 [Q8TB24-1] |
GeneIDi | 79890 |
KEGGi | hsa:79890 |
UCSCi | uc001yap.4, human [Q8TB24-1] |
Organism-specific databases
CTDi | 79890 |
DisGeNETi | 79890 |
EuPathDBi | HostDB:ENSG00000100599.15 |
GeneCardsi | RIN3 |
HGNCi | HGNC:18751, RIN3 |
HPAi | ENSG00000100599, Low tissue specificity |
MIMi | 610223, gene |
neXtProti | NX_Q8TB24 |
NIAGADSi | ENSG00000100599 |
OpenTargetsi | ENSG00000100599 |
PharmGKBi | PA38673 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2320, Eukaryota |
GeneTreei | ENSGT00940000158622 |
InParanoidi | Q8TB24 |
OMAi | QEIRMMM |
PhylomeDBi | Q8TB24 |
TreeFami | TF331067 |
Enzyme and pathway databases
PathwayCommonsi | Q8TB24 |
Reactomei | R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
Miscellaneous databases
BioGRID-ORCSi | 79890, 4 hits in 840 CRISPR screens |
ChiTaRSi | RIN3, human |
GenomeRNAii | 79890 |
Pharosi | Q8TB24, Tbio |
PROi | PR:Q8TB24 |
RNActi | Q8TB24, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100599, Expressed in blood and 183 other tissues |
ExpressionAtlasi | Q8TB24, baseline and differential |
Genevisiblei | Q8TB24, HS |
Family and domain databases
CDDi | cd10395, SH2_RIN3, 1 hit |
Gene3Di | 1.20.1050.80, 1 hit 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR000159, RA_dom IPR035869, RIN3_SH2 IPR000980, SH2 IPR036860, SH2_dom_sf IPR003123, VPS9 IPR037191, VPS9_dom_sf |
Pfami | View protein in Pfam PF02204, VPS9, 1 hit |
SMARTi | View protein in SMART SM00252, SH2, 1 hit SM00167, VPS9, 1 hit |
SUPFAMi | SSF109993, SSF109993, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50200, RA, 1 hit PS50001, SH2, 1 hit PS51205, VPS9, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RIN3_HUMAN | |
Accessioni | Q8TB24Primary (citable) accession number: Q8TB24 Secondary accession number(s): Q76LB3 Q9HAG1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2003 |
Last sequence update: | October 14, 2008 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 14
Human chromosome 14: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations