UniProtKB - Q8T9Z7 (PNPH_PLAFA)
Purine nucleoside phosphorylase
PNP
Functioni
As part of the purine salvage pathway, catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate (PubMed:11707439, PubMed:30602534).
Preferentially acts on inosine and guanosine, and to a lesser extent on 2'-deoxyinosine and 2'-deoxyguanosine (PubMed:11707439).
Also catalyzes the phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine; MTI is produced by adenosine deaminase (ADA)-mediated breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis (By similarity).
Generates hypoxanthine from both the purine salvage pathway and from polyamine metabolism which is required for nucleic acids synthesis (By similarity).
Has no activity towards adenosine (PubMed:11707439).
By similarity2 PublicationsCatalytic activityi
- EC:2.4.2.12 Publications
- EC:2.4.2.11 Publication
- EC:2.4.2.11 Publication
- EC:2.4.2.11 Publication
- phosphate + S-methyl-5'-thioinosine = hypoxanthine + S-methyl-5-thio-α-D-ribose 1-phosphateBy similarityEC:2.4.2.44By similarity
Activity regulationi
Kineticsi
- KM=5 µM for inosine1 Publication
- KM=104 µM for 2'-deoxyinosine1 Publication
- KM=9.7 µM for guanosine1 Publication
- KM=69 µM for 2'-deoxyguanosine1 Publication
- KM=85 µM for uridine1 Publication
: purine nucleoside salvage Pathwayi
This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 7 | Purine nucleoside; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 45 | Phosphate; shared with dimeric partnerBy similarity | 1 | |
Active sitei | 206 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- guanosine phosphorylase activity Source: RHEA
- purine-nucleoside phosphorylase activity Source: UniProtKB-EC
GO - Biological processi
- purine ribonucleoside salvage Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine salvage |
Enzyme and pathway databases
BRENDAi | 2.4.2.1, 4889 |
UniPathwayi | UPA00606 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PNPImported |
Organismi | Plasmodium falciparumImported |
Taxonomic identifieri | 5833 [NCBI] |
Taxonomic lineagei | Eukaryota › Sar › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodiidae › Plasmodium › Plasmodium (Laverania) |
Organism-specific databases
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000451826 | 1 – 245 | Purine nucleoside phosphorylaseAdd BLAST | 245 |
Interactioni
Subunit structurei
Homohexamer; trimer of homodimers.
By similarityChemistry databases
BindingDBi | Q8T9Z7 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q8T9Z7 |
SMRi | Q8T9Z7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 23 – 27 | Phosphate bindingCombined sources1 Publication | 5 | |
Regioni | 88 – 91 | Phosphate bindingCombined sources1 Publication | 4 | |
Regioni | 183 – 184 | Purine nucleoside bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
OMAi | MSDVFHL |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDNLLRHLKI SKEQITPVVL VVGDPGRVDK IKVVCDSYVD LAYNREYKSV
60 70 80 90 100
ECHYKGQKFL CVSHGVGSAG CAVCFEELCQ NGAKVIIRAG SCGSLQPDLI
110 120 130 140 150
KRGDICICNA AVREDRVSHL LIHGDFPAVG DFDVYDTLNK CAQELNVPVF
160 170 180 190 200
NGISVSSDMY YPNKIIPSRL EDYSKANAAV VEMELATLMV IGTLRKVKTG
210 220 230 240
GILIVDGCPF KWDEGDFDNN LVPHQLENMI KIALGACAKL ATKYA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF426159 Genomic DNA Translation: AAL74412.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF426159 Genomic DNA Translation: AAL74412.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5ZNC | X-ray | 1.66 | A | 1-245 | [»] | |
5ZNI | X-ray | 2.30 | A | 1-245 | [»] | |
AlphaFoldDBi | Q8T9Z7 | |||||
SMRi | Q8T9Z7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
BindingDBi | Q8T9Z7 |
ChEMBLi | CHEMBL5648 |
DrugCentrali | Q8T9Z7 |
Organism-specific databases
Phylogenomic databases
OMAi | MSDVFHL |
Enzyme and pathway databases
UniPathwayi | UPA00606 |
BRENDAi | 2.4.2.1, 4889 |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PNPH_PLAFA | |
Accessioni | Q8T9Z7Primary (citable) accession number: Q8T9Z7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 10, 2021 |
Last sequence update: | June 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 82 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families