UniProtKB - Q8T6B9 (PUF68_DROME)
Protein
Poly(U)-binding-splicing factor half pint
Gene
hfp
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Splicing factor that regulates oogenesis and controls both mitosis and mRNA localization in the germline by regulating mRNA splicing of a subset of genes within the ovary. Probably acts by regulating the alternative splice site selection of the otu transcript. Also regulates the alternative splicing of eIF4E1 and grk, while it is not involved in the splicing of par-1, sqd and psq.1 Publication
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- alternative mRNA splicing, via spliceosome Source: FlyBase
- cystoblast division Source: FlyBase
- mRNA splice site selection Source: FlyBase
- mRNA splicing, via spliceosome Source: FlyBase
- multicellular organism development Source: UniProtKB-KW
- negative regulation of cell population proliferation Source: FlyBase
- negative regulation of growth Source: FlyBase
- oogenesis Source: UniProtKB-KW
- regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
- regulation of cell growth Source: FlyBase
Keywordsi
Molecular function | Developmental protein, RNA-binding |
Biological process | Differentiation, mRNA processing, mRNA splicing, Oogenesis |
Enzyme and pathway databases
Reactomei | R-DME-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Poly(U)-binding-splicing factor half pint1 PublicationShort name: Protein half pint1 Publication Alternative name(s): 68 kDa poly(U)-binding-splicing factorCurated PUF60 homolog1 Publication |
Gene namesi | ORF Names:CG12085Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0028577, hfp |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- catalytic step 2 spliceosome Source: FlyBase
- nucleus Source: FlyBase
- precatalytic spliceosome Source: FlyBase
Other locations
- protein-containing complex Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081744 | 1 – 637 | Poly(U)-binding-splicing factor half pintAdd BLAST | 637 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | Phosphoserine1 Publication | 1 | |
Modified residuei | 30 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8T6B9 |
PRIDEi | Q8T6B9 |
PTM databases
iPTMneti | Q8T6B9 |
Expressioni
Tissue specificityi
Expressed in all germline cells and within the follicle cell.1 Publication
Gene expression databases
Bgeei | FBgn0028577, Expressed in brain and 47 other tissues |
ExpressionAtlasi | Q8T6B9, baseline and differential |
Genevisiblei | Q8T6B9, DM |
Interactioni
Subunit structurei
Interacts with enc. However, given the cytoplasmic localization of enc, the relevance of such interaction is unclear.
1 PublicationProtein-protein interaction databases
BioGRIDi | 63711, 56 interactors |
IntActi | Q8T6B9, 15 interactors |
MINTi | Q8T6B9 |
STRINGi | 7227.FBpp0072593 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 130 – 208 | RRM 1PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 227 – 305 | RRM 2PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 537 – 627 | RRM 3; atypicalPROSITE-ProRule annotationAdd BLAST | 91 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 322 – 479 | Ala-richAdd BLAST | 158 |
Domaini
The third RNA recognition motif, called PUMP domain in PubMed:10606266, is atypical and may rather mediate protein-protein interactions.1 Publication
Sequence similaritiesi
Belongs to the RRM half pint family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0124, Eukaryota |
GeneTreei | ENSGT00940000155594 |
InParanoidi | Q8T6B9 |
PhylomeDBi | Q8T6B9 |
Family and domain databases
CDDi | cd12370, RRM1_PUF60, 1 hit cd12371, RRM2_PUF60, 1 hit cd12648, RRM3_UHM_PUF60, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR006532, PolyU-bd IPR034209, PUF60_RRM1 IPR034211, PUF60_RRM2 IPR034212, PUF60_RRM3 IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits SM00361, RRM_1, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
TIGRFAMsi | TIGR01645, half-pint, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform A (identifier: Q8T6B9-1) [UniParc]FASTAAdd to basket
Also known as: Long
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSNDRASRS PRSDDQREIS DMPATKRTRS DSGKSTDSKI PYLSQPLYDL
60 70 80 90 100
KQTGDVKFGP GTRSALLGLL GGALPKLSSE QHDLVSKAKK YAMEQSIKMV
110 120 130 140 150
LMKQTLAHQQ QQLATQRTQV QRQQALALMC RVYVGSISFE LKEDTIRVAF
160 170 180 190 200
TPFGPIKSIN MSWDPITQKH KGFAFVEYEI PEGAQLALEQ MNGALMGGRN
210 220 230 240 250
IKVGRPSNMP QAQQVIDEVQ EEAKSFNRIY VASIHPDLSE EDIKSVFEAF
260 270 280 290 300
GPILYCKLAQ GTSLHTHKGY GFIEYANKQA MDEAIASMNL FDLGGQLLRV
310 320 330 340 350
GRSITPPNAL ACPTTNSTMP TAAAVAAAAA TAKIQALDAV ASNAVLGLSQ
360 370 380 390 400
NTPVMAAGAV VTKVGAMPVV SAATSAAALH PALAQAAPAL LPPGIFQAPT
410 420 430 440 450
PVAPSLLGVP AGLQPLQAVV PTLPPPALLA TPTLPMTVGG VGVGLVPTVA
460 470 480 490 500
TLAGAEASKG AAAAAALSAA ANNAAVTAAN LSENIKKAHE KQQEELQKKL
510 520 530 540 550
MDEGDVQTLQ QQENMSIKGQ SARQLVMQRL MRPVDSRVII LRNMVGPEDV
560 570 580 590 600
DETLQEEIQE ECSKFGTVSR VIIFNEKQTE NEDDDEAEII VKIFVEFSAG
610 620 630
AEAMRGKEAL DGRFFGGRRV VAELYDQGIF DQGDLSG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA4V193 | A4V193_DROME | Half pint, isoform D | hfp anon-WO0118547.408, dFIR, Dmel\CG12085, DPUF68, Fir | 545 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 583 | D → A in AAL86452 (PubMed:11879639).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009328 | 1 – 92 | Missing in isoform B. 1 PublicationAdd BLAST | 92 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF190745 mRNA Translation: AAF04132.1 AF479079 mRNA Translation: AAL86452.1 AE014296 Genomic DNA Translation: AAF47501.1 AE014296 Genomic DNA Translation: AAG22221.1 AY061006 mRNA Translation: AAL28554.1 BT003317 mRNA Translation: AAO25077.1 |
RefSeqi | NP_001163315.1, NM_001169844.2 [Q8T6B9-2] NP_001163316.1, NM_001169845.2 [Q8T6B9-2] NP_001163317.1, NM_001169846.2 [Q8T6B9-2] NP_001163318.1, NM_001169847.2 [Q8T6B9-2] NP_001163319.1, NM_001169848.2 [Q8T6B9-2] NP_001163320.1, NM_001169849.2 [Q8T6B9-2] NP_525123.2, NM_080384.4 [Q8T6B9-1] NP_728610.1, NM_167880.3 [Q8T6B9-2] NP_728611.1, NM_167881.3 [Q8T6B9-2] NP_728612.1, NM_167882.3 [Q8T6B9-2] |
Genome annotation databases
EnsemblMetazoai | FBtr0072707; FBpp0072591; FBgn0028577 [Q8T6B9-2] FBtr0072708; FBpp0072592; FBgn0028577 [Q8T6B9-2] FBtr0072709; FBpp0072593; FBgn0028577 [Q8T6B9-1] FBtr0072710; FBpp0072594; FBgn0028577 [Q8T6B9-2] FBtr0300416; FBpp0289645; FBgn0028577 [Q8T6B9-2] FBtr0300417; FBpp0289646; FBgn0028577 [Q8T6B9-2] FBtr0300418; FBpp0289647; FBgn0028577 [Q8T6B9-2] FBtr0300419; FBpp0289648; FBgn0028577 [Q8T6B9-2] FBtr0300420; FBpp0289649; FBgn0028577 [Q8T6B9-2] FBtr0301523; FBpp0290738; FBgn0028577 [Q8T6B9-2] |
GeneIDi | 38173 |
KEGGi | dme:Dmel_CG12085 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF190745 mRNA Translation: AAF04132.1 AF479079 mRNA Translation: AAL86452.1 AE014296 Genomic DNA Translation: AAF47501.1 AE014296 Genomic DNA Translation: AAG22221.1 AY061006 mRNA Translation: AAL28554.1 BT003317 mRNA Translation: AAO25077.1 |
RefSeqi | NP_001163315.1, NM_001169844.2 [Q8T6B9-2] NP_001163316.1, NM_001169845.2 [Q8T6B9-2] NP_001163317.1, NM_001169846.2 [Q8T6B9-2] NP_001163318.1, NM_001169847.2 [Q8T6B9-2] NP_001163319.1, NM_001169848.2 [Q8T6B9-2] NP_001163320.1, NM_001169849.2 [Q8T6B9-2] NP_525123.2, NM_080384.4 [Q8T6B9-1] NP_728610.1, NM_167880.3 [Q8T6B9-2] NP_728611.1, NM_167881.3 [Q8T6B9-2] NP_728612.1, NM_167882.3 [Q8T6B9-2] |
3D structure databases
SMRi | Q8T6B9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63711, 56 interactors |
IntActi | Q8T6B9, 15 interactors |
MINTi | Q8T6B9 |
STRINGi | 7227.FBpp0072593 |
PTM databases
iPTMneti | Q8T6B9 |
Proteomic databases
PaxDbi | Q8T6B9 |
PRIDEi | Q8T6B9 |
Genome annotation databases
EnsemblMetazoai | FBtr0072707; FBpp0072591; FBgn0028577 [Q8T6B9-2] FBtr0072708; FBpp0072592; FBgn0028577 [Q8T6B9-2] FBtr0072709; FBpp0072593; FBgn0028577 [Q8T6B9-1] FBtr0072710; FBpp0072594; FBgn0028577 [Q8T6B9-2] FBtr0300416; FBpp0289645; FBgn0028577 [Q8T6B9-2] FBtr0300417; FBpp0289646; FBgn0028577 [Q8T6B9-2] FBtr0300418; FBpp0289647; FBgn0028577 [Q8T6B9-2] FBtr0300419; FBpp0289648; FBgn0028577 [Q8T6B9-2] FBtr0300420; FBpp0289649; FBgn0028577 [Q8T6B9-2] FBtr0301523; FBpp0290738; FBgn0028577 [Q8T6B9-2] |
GeneIDi | 38173 |
KEGGi | dme:Dmel_CG12085 |
Organism-specific databases
CTDi | 38173 |
FlyBasei | FBgn0028577, hfp |
Phylogenomic databases
eggNOGi | KOG0124, Eukaryota |
GeneTreei | ENSGT00940000155594 |
InParanoidi | Q8T6B9 |
PhylomeDBi | Q8T6B9 |
Enzyme and pathway databases
Reactomei | R-DME-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 38173, 0 hits in 1 CRISPR screen |
ChiTaRSi | pUf68, fly |
GenomeRNAii | 38173 |
PROi | PR:Q8T6B9 |
Gene expression databases
Bgeei | FBgn0028577, Expressed in brain and 47 other tissues |
ExpressionAtlasi | Q8T6B9, baseline and differential |
Genevisiblei | Q8T6B9, DM |
Family and domain databases
CDDi | cd12370, RRM1_PUF60, 1 hit cd12371, RRM2_PUF60, 1 hit cd12648, RRM3_UHM_PUF60, 1 hit |
Gene3Di | 3.30.70.330, 3 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR006532, PolyU-bd IPR034209, PUF60_RRM1 IPR034211, PUF60_RRM2 IPR034212, PUF60_RRM3 IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 3 hits SM00361, RRM_1, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
TIGRFAMsi | TIGR01645, half-pint, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUF68_DROME | |
Accessioni | Q8T6B9Primary (citable) accession number: Q8T6B9 Secondary accession number(s): Q0E8J8 Q9W0E7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | February 2, 2004 | |
Last modified: | December 2, 2020 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families