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Protein

Lysozyme A

Gene

alyA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has antibacterial activity against the Gram-positive bacteria B.subtilis, B.megaterium and M.luteus. No antibacterial activity detected against the Gram-positive bacterium S.aureus or against the Gram-negative bacterium E.coli. Lacks chitinase activity.1 Publication

Catalytic activityi

Hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan.1 Publication

Temperature dependencei

Stable when incubated at 50 degrees Celsius for 10 minutes at pH 5.5. Activity decreases by 50% when incubated at 60 degrees Celsius for 10 minutes at pH 5.5.1 Publication

GO - Molecular functioni

  • lysozyme activity Source: UniProtKB

GO - Biological processi

  • cytolysis Source: UniProtKB-KW
  • defense response to Gram-positive bacterium Source: UniProtKB
  • peptidoglycan catabolic process Source: dictyBase
  • phagocytosis Source: dictyBase

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lysozyme A (EC:3.2.1.n3)
Alternative name(s):
1,4-beta-N-acetylmuramidase A
Gene namesi
Name:alyAImported
ORF Names:DDB_G0275123
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0275123 alyA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasmic vesicle

Pathology & Biotechi

Disruption phenotypei

Mutants have only 40% of the wild-type lysozyme activity and initially form plaques on bacterial lawns that are only half the size of wild type plaques. Despite the lysozyme activity remaining constantly at 40% of wild-type, the efficiency of plaque formation gradually increases over several weeks repeated plating until the size of mutant plaques finally exceeds that of wild-type cells by almost 2-fold.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000031591420 – 138Lysozyme A1 PublicationAdd BLAST119
PropeptideiPRO_0000315915139 – 1811 PublicationAdd BLAST43

Post-translational modificationi

Contains six disulfide bonds.Curated1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8T1G4

Family & Domainsi

Sequence similaritiesi

Belongs to the dictyostelium lysozyme family.Sequence analysis

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ8T1G4
OMAiFGCGKYL
PhylomeDBiQ8T1G4

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8T1G4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRIAFFLLVL AVIIGFAYGY SCPKPCYGNM CCSTSPNNQY YLTDFCGSTS
60 70 80 90 100
ACGPVPSCSG ALYFTADSQR FGCGKYLNLC RSGKCVKAQI YDAGPAMWVE
110 120 130 140 150
QDAGMMIIDA SPTICHVLTG GSSCGWSDHF LITATVTSLT DSRPLGPFNV
160 170 180
TESEMAQLFI DHEIAMAQCE AEKTCNGFDL E
Length:181
Mass (Da):19,526
Last modified:June 1, 2002 - v1
Checksum:i27B032BE137C5A3F
GO

Mass spectrometryi

Molecular mass is 12652 Da from positions 20 - 138. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA Translation: EAL69842.1
C92846 mRNA No translation available.
RefSeqiXP_643739.1, XM_638647.1

Genome annotation databases

EnsemblProtistsiEAL69842; EAL69842; DDB_G0275123
GeneIDi8619783
KEGGiddi:DDB_G0275123

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000013 Genomic DNA Translation: EAL69842.1
C92846 mRNA No translation available.
RefSeqiXP_643739.1, XM_638647.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ8T1G4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL69842; EAL69842; DDB_G0275123
GeneIDi8619783
KEGGiddi:DDB_G0275123

Organism-specific databases

dictyBaseiDDB_G0275123 alyA

Phylogenomic databases

InParanoidiQ8T1G4
OMAiFGCGKYL
PhylomeDBiQ8T1G4

Miscellaneous databases

PROiPR:Q8T1G4

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiLYSA_DICDI
AccessioniPrimary (citable) accession number: Q8T1G4
Secondary accession number(s): Q554H2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 1, 2002
Last modified: February 28, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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