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Protein

Protein Gawky

Gene

gw

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for gene silencing mediated by micro-RNAs (miRNAs). Silences both polyadenylated and deadenylated mRNAs. Required for miRNA-mediated translational repression and mRNA decay. Not required for miRNA target recognition. Necessary to initiate but not to maintain silencing. Promotes mRNA deadenylation through the recruitment of the CCR4-NOT and PAN complexes and promotes decapping by the DCP1-DCP2 complex. Dissociates from silenced mRNAs after deadenylation. Required for completion of nuclear divisions during early embryonic development.6 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, RNA-binding
Biological processRNA-mediated gene silencing, Translation regulation

Enzyme and pathway databases

ReactomeiR-DME-426496 Post-transcriptional silencing by small RNAs
R-DME-5578749 Transcriptional regulation by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Protein GawkyImported
Gene namesi
Name:gw
Synonyms:GW1821 Publication
ORF Names:CG31992
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 4

Organism-specific databases

FlyBaseiFBgn0051992 gw

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004159481 – 1384Protein GawkyAdd BLAST1384

Proteomic databases

PaxDbiQ8SY33
PRIDEiQ8SY33

Expressioni

Developmental stagei

Highest levels are found during early embryonic development until approximately 18 hours and during pupariation.1 Publication

Gene expression databases

BgeeiFBgn0051992 Expressed in 31 organ(s), highest expression level in embryo
GenevisibleiQ8SY33 DM

Interactioni

Subunit structurei

Interacts (via N-terminal region) with AGO1 (via Piwi domain); the interaction is essential for localization of AGO1 in P-bodies and for miRNA-mediated silencing. Interacts with pAbp/PABPC1; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing. Interacts with CCR4-NOT complex members Not1, Rga/NOT2, twin/CCR4, Pop2 and NOT3/5 and with PAN complex members CG8232/PAN2 and CG11486/PAN3.4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi68639, 20 interactors
DIPiDIP-35525N
ELMiQ8SY33
IntActiQ8SY33, 12 interactors
MINTiQ8SY33
STRINGi7227.FBpp0088165

Structurei

Secondary structure

11384
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ8SY33
SMRiQ8SY33
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8SY33

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini547 – 588UBAPROSITE-ProRule annotationAdd BLAST42
Domaini1117 – 1189RRMSequence analysisAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 605Sufficient for miRNA-mediated silencing1 PublicationAdd BLAST605
Regioni1 – 205Required for interaction with AGO11 PublicationAdd BLAST205
Regioni205 – 490Minimal N-terminal region required for miRNA-mediated silencing1 PublicationAdd BLAST286
Regioni605 – 830Sufficient for miRNA-mediated silencing1 PublicationAdd BLAST226
Regioni862 – 1115Not required for interaction with AGO1 or miRNAs or for localization to P-bodies but necessary for miRNA-mediated silencing and for interaction with pAbp3 PublicationsAdd BLAST254
Regioni940 – 1215Sufficient for miRNA-mediated silencing1 PublicationAdd BLAST276
Regioni1200 – 1384Not required for interaction with AGO1 or miRNAs or for localization to P-bodies but necessary for miRNA-mediated silencing, dissociation from AGO1 and miRNAs and interaction with pAbp3 PublicationsAdd BLAST185

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi81 – 281Gly-richSequence analysisAdd BLAST201
Compositional biasi527 – 530Poly-GlnSequence analysis4
Compositional biasi713 – 824Gln-richSequence analysisAdd BLAST112
Compositional biasi1204 – 1366Ser-richSequence analysisAdd BLAST163

Domaini

The UBA domain is not required for correct subcellular location, gene silencing or interaction with pAbp.1 Publication
The RRM domain lacks RNA-binding properties and does not bind RNA in vitro. It is not required for P-body localization or for interaction with AGO1 or miRNAs but is required for silencing. May play a role in protein-protein interactions.1 Publication1 Publication

Sequence similaritiesi

Belongs to the GW182 family.Sequence analysis

Phylogenomic databases

eggNOGiENOG410IFAU Eukaryota
ENOG41110AX LUCA
GeneTreeiENSGT00410000025966
InParanoidiQ8SY33
KOiK18412
OMAiNCVLSNT
OrthoDBiEOG091G0JQM
PhylomeDBiQ8SY33

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR019486 Argonaute_hook_dom
IPR033503 GW182
IPR026805 GW182_M_dom
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR015940 UBA
IPR009060 UBA-like_sf
PANTHERiPTHR13020:SF25 PTHR13020:SF25, 1 hit
PfamiView protein in Pfam
PF10427 Ago_hook, 1 hit
PF12938 M_domain, 1 hit
PF00627 UBA, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform A (identifier: Q8SY33-1) [UniParc]FASTAAdd to basket
Also known as: B, E, F

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MREALFSQDG WGCQHVNQDT NWEVPSSPEP ANKDAPGPPM WKPSINNGTD
60 70 80 90 100
LWESNLRNGG QPAAQQVPKP SWGHTPSSNL GGTWGEDDDG ADSSSVWTGG
110 120 130 140 150
AVSNAGSGAA VGVNQAGVNV GPGGVVSSGG PQWGQGVVGV GLGSTGGNGS
160 170 180 190 200
SNITGSSGVA TGSSGNSSNA GNGWGDPREI RPLGVGGSMD IRNVEHRGGN
210 220 230 240 250
GSGATSSDPR DIRMIDPRDP IRGDPRGISG RLNGTSEMWG HHPQMSHNQL
260 270 280 290 300
QGINKMVGQS VATASTSVGT SGSGIGPGGP GPSTVSGNIP TQWGPAQPVS
310 320 330 340 350
VGVSGPKDMS KQISGWEEPS PPPQRRSIPN YDDGTSLWGQ QTRVPAASGH
360 370 380 390 400
WKDMTDSIGR SSHLMRGQSQ TGGIGIAGVG NSNVPVGANP SNPISSVVGP
410 420 430 440 450
QARIPSVGGV QHKPDGGAMW VHSGNVGGRN NVAAVTTWGD DTHSVNVGAP
460 470 480 490 500
SSGSVSSNNW VDDKSNSTLA QNSWSDPAPV GVSWGNKQSK PPSNSASSGW
510 520 530 540 550
STAAGVVDGV DLGSEWNTHG GIIGKSQQQQ KLAGLNVGMV NVINAEIIKQ
560 570 580 590 600
SKQYRILVEN GFKKEDVERA LVIANMNIEE AADMLRANSS LSMDGWRRHD
610 620 630 640 650
ESLGSYADHN SSTSSGGFAG RYPVNSGQPS MSFPHNNLMN NMGGTAVTGG
660 670 680 690 700
NNNTNMTALQ VQKYLNQGQH GVAVGPQAVG NSSAVSVGFG QNTSNAAVAG
710 720 730 740 750
AASVNIAANT NNQPSGQQIR MLGQQIQLAI HSGFISSQIL TQPLTQTTLN
760 770 780 790 800
LLNQLLSNIK HLQAAQQSLT RGGNVNPMAV NVAISKYKQQ IQNLQNQINA
810 820 830 840 850
QQAVYVKQQN MQPTSQQQQP QQQQLPSVHL SNSGNDYLRG HDAINNLQSN
860 870 880 890 900
FSELNINKPS GYQGASNQQS RLNQWKLPVL DKEINSDSTE FSRAPGATKQ
910 920 930 940 950
NLTANTSNIN SLGLQNDSTW STGRSIGDGW PDPSSDNENK DWSVAQPTSA
960 970 980 990 1000
ATAYTDLVQE FEPGKPWKGS QIKSIEDDPS ITPGSVARSP LSINSTPKDA
1010 1020 1030 1040 1050
DIFANTGKNS PTDLPPLSLS SSTWSFNPNQ NYPSHSWSDN SQQCTATSEL
1060 1070 1080 1090 1100
WTSPLNKSSS RGPPPGLTAN SNKSANSNAS TPTTITGGAN GWLQPRSGGV
1110 1120 1130 1140 1150
QTTNTNWTGG NTTWGSSWLL LKNLTAQIDG PTLRTLCMQH GPLVSFHPYL
1160 1170 1180 1190 1200
NQGIALCKYT TREEANKAQM ALNNCVLANT TIFAESPSEN EVQSIMQHLP
1210 1220 1230 1240 1250
QTPSSTSSSG TSGGNVGGVG TSANNANSGS AACLSGNNSG NGNGSASGAG
1260 1270 1280 1290 1300
SGNNGNSSCN NSAAGGGSSS NNTITTVANS NLVGSSGSVS NSSGVTANSS
1310 1320 1330 1340 1350
TVSVVSCTAS GNSINGAGTA NSSGSKSSAN NLASGQSSAS NLTNSTNSTW
1360 1370 1380
RQTSQNQALQ SQSRPSGREA DFDYISLVYS IVDD
Length:1,384
Mass (Da):142,973
Last modified:June 1, 2002 - v1
Checksum:i4E87DFE49050883E
GO
Isoform I (identifier: Q8SY33-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     969-971: Missing.

Note: No experimental confirmation available.
Show »
Length:1,381
Mass (Da):142,701
Checksum:i9823EFFFE67253C4
GO
Isoform J (identifier: Q8SY33-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1035-1036: Missing.

Note: No experimental confirmation available.
Show »
Length:1,382
Mass (Da):142,749
Checksum:iBCBC248F4836B070
GO

Sequence cautioni

The sequence AAM50720 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047895969 – 971Missing in isoform I. Curated3
Alternative sequenceiVSP_0478961035 – 1036Missing in isoform J. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014135 Genomic DNA Translation: AAF59322.2
AE014135 Genomic DNA Translation: AAF59323.2
AE014135 Genomic DNA Translation: AAN06507.2
AE014135 Genomic DNA Translation: AAN06508.1
AE014135 Genomic DNA Translation: AAN06509.1
AE014135 Genomic DNA Translation: AAX52511.2
AY075429 mRNA Translation: AAL68245.1
AY118860 mRNA Translation: AAM50720.1 Different initiation.
RefSeqiNP_001014691.2, NM_001014691.2 [Q8SY33-3]
NP_726596.1, NM_166780.2 [Q8SY33-1]
NP_726597.1, NM_166781.2 [Q8SY33-1]
NP_726599.2, NM_166783.2 [Q8SY33-2]
NP_726600.1, NM_166784.2 [Q8SY33-1]
NP_726601.1, NM_166785.2 [Q8SY33-1]
UniGeneiDm.1403

Genome annotation databases

EnsemblMetazoaiFBtr0089096; FBpp0088165; FBgn0051992 [Q8SY33-1]
FBtr0089097; FBpp0088166; FBgn0051992 [Q8SY33-1]
FBtr0089100; FBpp0088169; FBgn0051992 [Q8SY33-1]
FBtr0089101; FBpp0088170; FBgn0051992 [Q8SY33-1]
FBtr0310542; FBpp0302679; FBgn0051992 [Q8SY33-2]
FBtr0310543; FBpp0302680; FBgn0051992 [Q8SY33-3]
GeneIDi43808
KEGGidme:Dmel_CG31992
UCSCiCG31992-RA d. melanogaster [Q8SY33-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014135 Genomic DNA Translation: AAF59322.2
AE014135 Genomic DNA Translation: AAF59323.2
AE014135 Genomic DNA Translation: AAN06507.2
AE014135 Genomic DNA Translation: AAN06508.1
AE014135 Genomic DNA Translation: AAN06509.1
AE014135 Genomic DNA Translation: AAX52511.2
AY075429 mRNA Translation: AAL68245.1
AY118860 mRNA Translation: AAM50720.1 Different initiation.
RefSeqiNP_001014691.2, NM_001014691.2 [Q8SY33-3]
NP_726596.1, NM_166780.2 [Q8SY33-1]
NP_726597.1, NM_166781.2 [Q8SY33-1]
NP_726599.2, NM_166783.2 [Q8SY33-2]
NP_726600.1, NM_166784.2 [Q8SY33-1]
NP_726601.1, NM_166785.2 [Q8SY33-1]
UniGeneiDm.1403

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WBRNMR-A1114-1198[»]
ProteinModelPortaliQ8SY33
SMRiQ8SY33
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68639, 20 interactors
DIPiDIP-35525N
ELMiQ8SY33
IntActiQ8SY33, 12 interactors
MINTiQ8SY33
STRINGi7227.FBpp0088165

Proteomic databases

PaxDbiQ8SY33
PRIDEiQ8SY33

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089096; FBpp0088165; FBgn0051992 [Q8SY33-1]
FBtr0089097; FBpp0088166; FBgn0051992 [Q8SY33-1]
FBtr0089100; FBpp0088169; FBgn0051992 [Q8SY33-1]
FBtr0089101; FBpp0088170; FBgn0051992 [Q8SY33-1]
FBtr0310542; FBpp0302679; FBgn0051992 [Q8SY33-2]
FBtr0310543; FBpp0302680; FBgn0051992 [Q8SY33-3]
GeneIDi43808
KEGGidme:Dmel_CG31992
UCSCiCG31992-RA d. melanogaster [Q8SY33-1]

Organism-specific databases

CTDi43808
FlyBaseiFBgn0051992 gw

Phylogenomic databases

eggNOGiENOG410IFAU Eukaryota
ENOG41110AX LUCA
GeneTreeiENSGT00410000025966
InParanoidiQ8SY33
KOiK18412
OMAiNCVLSNT
OrthoDBiEOG091G0JQM
PhylomeDBiQ8SY33

Enzyme and pathway databases

ReactomeiR-DME-426496 Post-transcriptional silencing by small RNAs
R-DME-5578749 Transcriptional regulation by small RNAs

Miscellaneous databases

ChiTaRSigw fly
EvolutionaryTraceiQ8SY33
GenomeRNAii43808
PROiPR:Q8SY33

Gene expression databases

BgeeiFBgn0051992 Expressed in 31 organ(s), highest expression level in embryo
GenevisibleiQ8SY33 DM

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR019486 Argonaute_hook_dom
IPR033503 GW182
IPR026805 GW182_M_dom
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR015940 UBA
IPR009060 UBA-like_sf
PANTHERiPTHR13020:SF25 PTHR13020:SF25, 1 hit
PfamiView protein in Pfam
PF10427 Ago_hook, 1 hit
PF12938 M_domain, 1 hit
PF00627 UBA, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGAWKY_DROME
AccessioniPrimary (citable) accession number: Q8SY33
Secondary accession number(s): Q8MSF0, Q9V4F1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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