UniProtKB - Q8SS85 (TDX_ENCCU)
Protein
Putative thioredoxin peroxidase
Gene
ECU03_1190
Organism
Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events.By similarity
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity
Catalytic activityi
- EC:1.11.1.24By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 45 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- peroxidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Protein family/group databases
PeroxiBasei | 5977, Ecu2CysPrx |
Names & Taxonomyi
Protein namesi | Recommended name: Putative thioredoxin peroxidase (EC:1.11.1.24By similarity)Alternative name(s): Peroxiredoxin Thioredoxin-dependent peroxide reductase Thioredoxin-dependent peroxiredoxinCurated |
Gene namesi | Ordered Locus Names:ECU03_1190 |
Organismi | Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) |
Taxonomic identifieri | 284813 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Fungi incertae sedis › Microsporidia › Unikaryonidae › Encephalitozoon › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | MicrosporidiaDB:ECU03_1190 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000383334 | 1 – 177 | Putative thioredoxin peroxidaseAdd BLAST | 177 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 45 | Interchain (with C-166); in linked formBy similarity | ||
Disulfide bondi | 166 | Interchain (with C-45); in linked formBy similarity |
Keywords - PTMi
Disulfide bondExpressioni
Developmental stagei
Expressed in late sporogonial stages.1 Publication
Interactioni
Subunit structurei
Homodimer; disulfide-linked, upon oxidation.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 158 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 158 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
HOGENOMi | CLU_042529_21_3_1 |
InParanoidi | Q8SS85 |
OMAi | WWPKDFT |
OrthoDBi | 1326484at2759 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q8SS85-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFPKTLTDSK YKAFVDGEIK EISLQDYIGK YVVLAFYPLD FTFVCPTEIN
60 70 80 90 100
RFSDLKGAFL RRNAVVLLIS CDSVYTHKAW ASIPREQNGV LGTAWPMVWD
110 120 130 140 150
AKRELCNQFG LYDEENGHPM RSTVILAKDL SVRHISSNYH AIGRSVDEII
160 170
RLIDAITFND ENGDICPAEW RSENKDN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL590443 Genomic DNA Translation: CAD26263.1 |
RefSeqi | NP_597628.1, NM_001040992.1 |
Genome annotation databases
GeneIDi | 858790 |
KEGGi | ecu:ECU03_1190 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL590443 Genomic DNA Translation: CAD26263.1 |
RefSeqi | NP_597628.1, NM_001040992.1 |
3D structure databases
SMRi | Q8SS85 |
ModBasei | Search... |
Protein family/group databases
PeroxiBasei | 5977, Ecu2CysPrx |
Genome annotation databases
GeneIDi | 858790 |
KEGGi | ecu:ECU03_1190 |
Organism-specific databases
VEuPathDBi | MicrosporidiaDB:ECU03_1190 |
Phylogenomic databases
HOGENOMi | CLU_042529_21_3_1 |
InParanoidi | Q8SS85 |
OMAi | WWPKDFT |
OrthoDBi | 1326484at2759 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TDX_ENCCU | |
Accessioni | Q8SS85Primary (citable) accession number: Q8SS85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 1, 2009 |
Last sequence update: | June 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families