UniProtKB - Q8SPU8 (DHRS4_BOVIN)
Dehydrogenase/reductase SDR family member 4
DHRS4
Functioni
NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones. Reduces all-trans-retinal and 9-cis retinal. Reduces 3-ketosteroids and benzil into 3alpha-hydroxysteroids and S-benzoin, respectively, in contrast to the stereoselectivity of primates DHRS4s which produce 3beta-hydroxysteroids and R-benzoin. In the reverse reaction, catalyzes the NADP-dependent oxidation of 3alpha-hydroxysteroids and alcohol, but with much lower efficiency. Involved in the metabolism of 3alpha-hydroxysteroids, retinoid, isatin and xenobiotic carbonyl compounds.
By similarityMiscellaneous
Catalytic activityi
- EC:1.1.1.184By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- EC:1.1.1.300By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 170 | SubstrateBy similarity | 1 | |
Sitei | 177 | Responsible for the stereoselective reduction of 3-ketosteroids into 3alpha-hydroxysteroids and benzil into S-benzoinBy similarity | 1 | |
Sitei | 180 | Responsible for the stereoselective reduction of 3-ketosteroids into 3alpha-hydroxysteroids and benzil into S-benzoinBy similarity | 1 | |
Active sitei | 183 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 187 | NADPBy similarity | 1 | |
Sitei | 196 | Important for the maintenance of the quaternary structure, the catalytic activity and cold stabilityBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 37 – 61 | NADPBy similarityAdd BLAST | 25 |
GO - Molecular functioni
- carbonyl reductase (NADPH) activity Source: UniProtKB-EC
- identical protein binding Source: UniProtKB
- NADP-retinol dehydrogenase activity Source: UniProtKB
GO - Biological processi
- cellular ketone metabolic process Source: UniProtKB
- retinoid metabolic process Source: UniProtKB
- steroid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Names & Taxonomyi
Protein namesi | Recommended name: Dehydrogenase/reductase SDR family member 4By similarity (EC:1.1.1.184By similarity, EC:1.1.1.300By similarity)Alternative name(s): NADPH-dependent carbonyl reductaseBy similarity Short name: CRBy similarity NADPH-dependent retinol dehydrogenase/reductaseBy similarity Short name: NDRDBy similarity Peroxisomal short-chain alcohol dehydrogenase Short name: PSCD Short chain dehydrogenase/reductase family 25C member 2By similarity Short name: Protein SDR25C2By similarity |
Gene namesi | Name:DHRS4 Synonyms:NDRD |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Peroxisome
- Peroxisome By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Peroxisome
- peroxisome Source: UniProtKB
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054646 | 1 – 279 | Dehydrogenase/reductase SDR family member 4Add BLAST | 279 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 93 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 93 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 106 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 221 | PhosphoserineBy similarity | 1 | |
Modified residuei | 235 | N6-succinyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q8SPU8 |
PeptideAtlasi | Q8SPU8 |
PRIDEi | Q8SPU8 |
Interactioni
Subunit structurei
Homotetramer.
By similarityGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
IntActi | Q8SPU8, 1 interactor |
STRINGi | 9913.ENSBTAP00000023493 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 277 – 279 | Peroxisomal targeting signal | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0725, Eukaryota |
InParanoidi | Q8SPU8 |
OrthoDBi | 1194344at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR029511, DHRS4-like IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PANTHERi | PTHR43943:SF8, PTHR43943:SF8, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLKVGLPLGA CARSWKSVRM ASCGMARRNP LDNKVALVTA STDGIGFAIA
60 70 80 90 100
RRLAQDGAHV VVSSRKQQNV DRAVATLKGE GLSVTGTVCH VGKAEDRERL
110 120 130 140 150
VATAVKLHGG VDILISNAAV SPFFGSLMDV PEEVWDKILD VNVKATALLT
160 170 180 190 200
KAVVPEMAKR GGGSIVIVSS IAAYSPFPSL GPYNVSKTAL LGLTKNLALE
210 220 230 240 250
LAESNVRVNC LAPGLIRTSF SRVLWEDPAR QESIKATFQI KRIGKPEECA
260 270
GIVSFLCSED ASYITGETVV VAGGSLSHL
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 | G → A in AAI12879 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112878 mRNA Translation: AAI12879.1 AF487454 mRNA Translation: AAL93248.1 Different initiation. |
RefSeqi | NP_777247.2, NM_174822.3 |
Genome annotation databases
GeneIDi | 281360 |
KEGGi | bta:281360 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112878 mRNA Translation: AAI12879.1 AF487454 mRNA Translation: AAL93248.1 Different initiation. |
RefSeqi | NP_777247.2, NM_174822.3 |
3D structure databases
AlphaFoldDBi | Q8SPU8 |
SMRi | Q8SPU8 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q8SPU8, 1 interactor |
STRINGi | 9913.ENSBTAP00000023493 |
Proteomic databases
PaxDbi | Q8SPU8 |
PeptideAtlasi | Q8SPU8 |
PRIDEi | Q8SPU8 |
Genome annotation databases
GeneIDi | 281360 |
KEGGi | bta:281360 |
Organism-specific databases
CTDi | 10901 |
Phylogenomic databases
eggNOGi | KOG0725, Eukaryota |
InParanoidi | Q8SPU8 |
OrthoDBi | 1194344at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR029511, DHRS4-like IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PANTHERi | PTHR43943:SF8, PTHR43943:SF8, 1 hit |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHRS4_BOVIN | |
Accessioni | Q8SPU8Primary (citable) accession number: Q8SPU8 Secondary accession number(s): Q2KHU6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2003 |
Last sequence update: | October 5, 2010 | |
Last modified: | May 25, 2022 | |
This is version 98 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families