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Protein

Photoactivated adenylate cyclase subunit alpha

Gene

pacA

Organism
Euglena gracilis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a blue light photoreceptor for the step-up photophobic response. Mediates photoavoidance.1 Publication

Miscellaneous

The 1224-5/9F strain is deficient in phototaxis. It is not known if this is due to defective adenylate cyclase activity or defective BLUF domains in the beta subunit of this protein.1 Publication

Catalytic activityi

ATP = 3',5'-cyclic AMP + diphosphate.1 Publication

Cofactori

FAD1 Publication

Enzyme regulationi

Activity increased by up to 80-fold under blue light.1 Publication

Kineticsi

  1. KM=0.5 µM for ATP1 Publication
  1. Vmax=3.5 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

  • adenylate cyclase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • blue light photoreceptor activity Source: InterPro
  • FAD binding Source: InterPro
  • photoreceptor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionLyase, Photoreceptor protein, Receptor
Biological processcAMP biosynthesis, Sensory transduction
LigandATP-binding, Chromophore, FAD, Flavoprotein, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Photoactivated adenylate cyclase subunit alpha (EC:4.6.1.1)
Alternative name(s):
Photoactivated adenylyl cyclase subunit alpha
Gene namesi
Name:pacAImported
OrganismiEuglena gracilis
Taxonomic identifieri3039 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaEuglenidaEuglenalesEuglenaceaeEuglena

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • motile cilium Source: UniProtKB

Keywords - Cellular componenti

Cell projection, Cilium, Flagellum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001957171 – 1019Photoactivated adenylate cyclase subunit alphaAdd BLAST1019

Proteomic databases

PRIDEiQ8S9F2

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ8S9F2
SMRiQ8S9F2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 148BLUF 1PROSITE-ProRule annotationAdd BLAST94
Domaini204 – 332Guanylate cyclase 1PROSITE-ProRule annotationAdd BLAST129
Domaini467 – 559BLUF 2PROSITE-ProRule annotationAdd BLAST93
Domaini615 – 744Guanylate cyclase 2PROSITE-ProRule annotationAdd BLAST130

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di3.30.70.1230, 2 hits
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR036046 Acylphosphatase-like_dom_sf
IPR007024 BLUF_domain
IPR032675 LRR_dom_sf
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF04940 BLUF, 2 hits
PF00211 Guanylate_cyc, 2 hits
SMARTiView protein in SMART
SM01034 BLUF, 2 hits
SUPFAMiSSF54975 SSF54975, 2 hits
SSF55073 SSF55073, 2 hits
PROSITEiView protein in PROSITE
PS50925 BLUF, 2 hits
PS50125 GUANYLATE_CYCLASE_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q8S9F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYILVWKEGQ QIRTFQDLEE CGQFQTASNI TDGQIFSINV TPTMSKGGET
60 70 80 90 100
GETQLRRLMY LSASTEPEKC NAEYLADMAH VATLRNKQIG VSGFLLYSSP
110 120 130 140 150
FFFQVIEGTD EDLDFLFAKI SADPRHERCI VLANGPCTGR MYGEWHMKDS
160 170 180 190 200
HIDNITKHPA IKTILFQIAR SFSSMWSYLP KNAANMLLLG KNPNKQAPEP
210 220 230 240 250
MSVVVTFIYL VEFSSILAHP GLTEQCADIL AAFVDACVRN VEGTGGQVAK
260 270 280 290 300
FITGICMAYW PINRAEDALV GLQQLSEDLA ELRSQQPPGS ALSLIYSRCG
310 320 330 340 350
VHYGRALLCN AGFRKADFTL LGDCINTASR ITSLSVKLKV PLLLSFEVRC
360 370 380 390 400
LLGDEMREEL ESSGLHKVKG RDKPVQVYQF NAPELDSAMV RAKIEQFNPG
410 420 430 440 450
RYRALCPVKP YESLHPAQRP PIFDDTPREN QPKLSQVQRR DSLVDRLSLI
460 470 480 490 500
AKLAFPSSMM AGGEGQLITL TYISQAAHPM SRLDLASIQR IAFARNESSN
510 520 530 540 550
ITGSLLYVSG LFVQTLEGPK GAVVSLYLKI RQDKRHKDVV AVFMAPIDER
560 570 580 590 600
VYGSPLDMTS ATEEMLATFP PLQDVLSQLA KSFISLETYV PSTVVRYLTA
610 620 630 640 650
GNNPRNLQPV SVEVVMLATD ICSFTPLSEK CSLTEVWTIC NTFIDACTSA
660 670 680 690 700
ICNEGGEVIK LIGDCVTAYF PPTGADNAVH ACQEIVSFCA QLRDAFHDVL
710 720 730 740 750
DCRSVVACGV GLDFGQVIMA QCGSLGMTEF VVAGEVSARV MEVEALTREA
760 770 780 790 800
GRAIVITEPV ADRLSPKLRD TGIVPCQEGV DGVPCYGILG PEWELDVATI
810 820 830 840 850
KKNIYGFHDA RALAAMKKVD DGTNAPGRGA PAGGIPSSPK VRPPGRTNSV
860 870 880 890 900
SSYTPDPNEA LDPRMAESVF LDMCHQRGDT ANNSIAVKLR QAANDDRLDL
910 920 930 940 950
GRMLQGPHEL MPVMQAIKHL TNLRMLNMSD NFVDDNNVGE LVESCIPMRS
960 970 980 990 1000
LQVLDLSNNP GLTKVIALKR LIKHNTQVRE ILLNGTRIAP TEQRKLQSSM
1010
NVNRLCASTD LKGSHKYEH
Length:1,019
Mass (Da):111,990
Last modified:June 1, 2002 - v1
Checksum:iC28F8B3D1EAB1F34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031225 mRNA Translation: BAB85619.1
AM181334 mRNA Translation: CAJ57393.1

Similar proteinsi

Entry informationi

Entry nameiPCYAA_EUGGR
AccessioniPrimary (citable) accession number: Q8S9F2
Secondary accession number(s): Q2P9N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2002
Last modified: July 18, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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