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Protein

Adenylosuccinate lyase

Gene

At4g18440

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
N6-(1,2-dicarboxyethyl)AMP = fumarate + AMP.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase, chloroplastic (PURA), Adenylosuccinate synthetase, chloroplastic (PURA), Adenylosuccinate synthetase, chloroplastic (PURA)
  2. Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (AXX17_At1g37010), Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (F15C21.8), Adenylosuccinate lyase (AXX17_At4g21680), Adenylosuccinate lyase (At4g18440)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic (PUR7)
  2. Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (AXX17_At1g37010), Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (F15C21.8), Adenylosuccinate lyase (AXX17_At4g21680), Adenylosuccinate lyase (At4g18440)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationImported
Biological processPurine biosynthesisUniRule annotation

Enzyme and pathway databases

BioCyciARA:AT4G18440-MONOMER
ReactomeiR-ATH-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00132
UPA00075; UER00336

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate lyaseUniRule annotation (EC:4.3.2.2UniRule annotation)
Short name:
ASLUniRule annotation
Alternative name(s):
AdenylosuccinaseUniRule annotation
Gene namesi
Ordered Locus Names:At4g18440Imported
ORF Names:F28J12.100Imported, F28J12_100Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G18440
TAIRilocus:2124494 AT4G18440

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Proteomic databases

PRIDEiQ8RY94

Expressioni

Gene expression databases

ExpressionAtlasiQ8RY94 baseline and differential

Interactioni

Protein-protein interaction databases

IntActiQ8RY94, 1 interactor
STRINGi3702.AT4G18440.1

Structurei

3D structure databases

SMRiQ8RY94
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 388Lyase_1InterPro annotationAdd BLAST263
Domaini404 – 518ASL_CInterPro annotationAdd BLAST115

Sequence similaritiesi

Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2700 Eukaryota
COG0015 LUCA
HOGENOMiHOG000252916
KOiK01756
OMAiFRVFVEI
OrthoDBiEOG093607L3

Family and domain databases

Gene3Di1.10.275.10, 1 hit
InterProiView protein in InterPro
IPR024083 Fumarase/histidase_N
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
IPR004769 Pur_lyase
IPR013539 PurB_C
PfamiView protein in Pfam
PF08328 ASL_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00928 purB, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q8RY94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELNFSSMAI THPKIPSFGF TPTGIFLNPS KSVCLASHHR LPRVSCSVST
60 70 80 90 100
TTDSPKLVTS TKVTAMDGVS SRDLEMSNLT ALSPLDGRYW SKVKDLASSL
110 120 130 140 150
SEFGLIYFRV FVEIKWLLKL SNIPEVTEVP SFSKEAQSFL QGIIDGFSID
160 170 180 190 200
DALEIKKIER VTNHDVKAVE YFLKQKCESQ PEIAKVLEFF HFACTSEDIN
210 220 230 240 250
NLSHALMLQE ALSSVILPTM DELIKSISLI AKNFAYVPML SRTHGQPATP
260 270 280 290 300
TTLGKEMANF AVRLSEERRY LSETKIKGKF AGAVGNYNAH ISAYSNIDWP
310 320 330 340 350
HVSEEFVTSL GLTFNPYVTQ IEPHDYMARL FNNISQFNTI LIDFDRDIWS
360 370 380 390 400
YISLGYFKQT TKAGEIGSST MPHKVNPIDF ENSEGNLGKA NAELTFLSMK
410 420 430 440 450
LPISRMQRDL TDSTVLRNMG GALGHSLLAY KSAIQGIGKL QVNEARLKED
460 470 480 490 500
LDDNWEVLAE PIQTVMRRYG VPEPYEKLKE LTRGKAVNEE TIRTFIKGLE
510 520 530
LPSEAKDQLL ELTPHTYVGA AAALALAVDE ALHLGH
Length:536
Mass (Da):59,757
Last modified:June 1, 2002 - v1
Checksum:i0D2157431FF2D590
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY074500 mRNA Translation: AAL69484.1
AY150476 mRNA Translation: AAN13001.1
CP002687 Genomic DNA Translation: AEE84046.1
RefSeqiNP_193579.2, NM_117957.5
UniGeneiAt.23844

Genome annotation databases

EnsemblPlantsiAT4G18440.1; AT4G18440.1; AT4G18440
GeneIDi827575
GrameneiAT4G18440.1; AT4G18440.1; AT4G18440
KEGGiath:AT4G18440

Similar proteinsi

Entry informationi

Entry nameiQ8RY94_ARATH
AccessioniPrimary (citable) accession number: Q8RY94
Entry historyiIntegrated into UniProtKB/TrEMBL: June 1, 2002
Last sequence update: June 1, 2002
Last modified: June 20, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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