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UniProtKB - Q8RXN3 (PPT1_ARATH)
Protein
Phosphoenolpyruvate/phosphate translocator 1, chloroplastic
Gene
PPT1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Phosphoenolpyruvate/phosphate translocator that transports phosphoenolpyruvate (PEP), 2-phosphoglycerate, 3-phosphoglycerate and dihydroxyacetone phosphate. Imports PEP to the chloroplast stroma as one substrate of the shikimate pathway, from which aromatic amino acids and a variety of secondary products derive. Required for correct leaf mesophyll cell development and expression of chlorophyll a/b binding protein 3 (CAB3).
4 PublicationsGO - Molecular functioni
- antiporter activity Source: GO_Central
- phosphoenolpyruvate:phosphate antiporter activity Source: UniProtKB
- phosphoglycerate transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- carbohydrate transport Source: UniProtKB-KW
- phosphoenolpyruvate transport Source: UniProtKB
- phosphoglycerate transmembrane transport Source: UniProtKB
Keywordsi
Biological process | Sugar transport, Transport |
Ligand | Pyruvate |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoenolpyruvate/phosphate translocator 1, chloroplasticShort name: AtPPT1 Alternative name(s): Protein CAB UNDEREXPRESSED 1 |
Gene namesi | Name:PPT1 Synonyms:CUE1 Ordered Locus Names:At5g33320 ORF Names:F19N2.40 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G33320 |
TAIRi | locus:2145944, AT5G33320 |
Subcellular locationi
Chloroplast
Chloroplast
- chloroplast Source: TAIR
- chloroplast envelope Source: TAIR
- chloroplast membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- plastid Source: TAIR
- plastid inner membrane Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 105 – 125 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 137 – 157 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 165 – 185 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 198 – 218 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 222 – 242 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 283 – 303 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 324 – 346 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 377 – 396 | HelicalSequence analysisAdd BLAST | 20 |
Keywords - Cellular componenti
Chloroplast, Membrane, PlastidPathology & Biotechi
Disruption phenotypei
Reticulate leaf phenotype. Reduced number of leaf palisade mesophyll cells and increased bundle sheath cells. Small chloroplasts in mesophyll cells. Reduced photosynthetic electron transport rate. Under-expression of CAB3 protein. Reduced levels of aromatic amino acids and phenolic compounds derived from shikimate pathway.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 251 | G → E in cue1-7; strong reticulate leaf phenotype. 1 Publication | 1 | |
Mutagenesisi | 265 | R → C in cue1-5; weak reticulate leaf phenotype. 1 Publication | 1 | |
Mutagenesisi | 388 | A → V in cue1-3; weak reticulate leaf phenotype. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 85 | ChloroplastCombined sources1 PublicationAdd BLAST | 85 | |
ChainiPRO_0000406099 | 86 – 408 | Phosphoenolpyruvate/phosphate translocator 1, chloroplasticAdd BLAST | 323 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 86 | N-acetylalanineCombined sources1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q8RXN3 |
PRIDEi | Q8RXN3 |
ProteomicsDBi | 226493 |
PTM databases
iPTMneti | Q8RXN3 |
Expressioni
Tissue specificityi
Expressed in root columella, lateral root cap and root vasculature tissue. In leaves, highly expressed in xylem parenchyma cells. In flowers, expressed in sepals, petals, filaments of the stamens, anthers and stigma.1 Publication
Gene expression databases
ExpressionAtlasi | Q8RXN3, baseline and differential |
Genevisiblei | Q8RXN3, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 18566, 3 interactors |
STRINGi | 3702.AT5G33320.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 124 – 241 | EamAAdd BLAST | 118 |
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1441, Eukaryota |
HOGENOMi | CLU_019048_0_0_1 |
InParanoidi | Q8RXN3 |
OMAi | FWYTVSS |
OrthoDBi | 1453018at2759 |
PhylomeDBi | Q8RXN3 |
Family and domain databases
InterProi | View protein in InterPro IPR004853, Sugar_P_trans_dom IPR004696, Tpt_PEP_transl |
Pfami | View protein in Pfam PF03151, TPT, 1 hit |
TIGRFAMsi | TIGR00817, tpt, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q8RXN3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQSSAVFSLS PSLPLLKPRR LSLRHHPITT AASSSDLNVS PNVVSIPSLS
60 70 80 90 100
RRSWRLASSD SPLRAWSGVP SPISHSLDTN RFRTAATAVP ESAEEGDNSG
110 120 130 140 150
KLTKVLELGL LFAMWYLFNI YFNIYNKQVL KALHAPMTVT LVQFAVGSVL
160 170 180 190 200
ITIMWVLNLY KRPKISGAQL AAILPLAVVH TLGNLFTNMS LGKVSVSFTH
210 220 230 240 250
TIKAMEPFFS VLLSAMFLGE KPTPWVLGAI VPIVGGVALA SISEVSFNWA
260 270 280 290 300
GFSSAMASNL TNQSRNVLSK KVMVKKDDSL DNITLFSIIT LMSLVLMAPV
310 320 330 340 350
TFFTEGIKFT PSYIQSAGVN VKQIYTKSLI AALCFHAYQQ VSYMILARVS
360 370 380 390 400
PVTHSVGNCV KRVVVIVSSV IFFKTPVSPV NAFGTGIALA GVFLYSRVKG
IKPKPKTA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 153 | I → T in AAF63704 (Ref. 1) Curated | 1 | |
Sequence conflicti | 191 | L → I in AAB40646 (Ref. 1) Curated | 1 | |
Sequence conflicti | 322 | K → Q in AAM63308 (Ref. 5) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U66321 mRNA Translation: AAB40646.1 AF209210 Genomic DNA Translation: AAF63704.1 AC051625 Genomic DNA No translation available. CP002688 Genomic DNA Translation: AED93894.1 AY080788 mRNA Translation: AAL87271.1 AY133809 mRNA Translation: AAM91743.1 AY086100 mRNA Translation: AAM63308.1 |
RefSeqi | NP_198317.1, NM_122856.3 |
Genome annotation databases
EnsemblPlantsi | AT5G33320.1; AT5G33320.1; AT5G33320 |
GeneIDi | 833308 |
Gramenei | AT5G33320.1; AT5G33320.1; AT5G33320 |
KEGGi | ath:AT5G33320 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U66321 mRNA Translation: AAB40646.1 AF209210 Genomic DNA Translation: AAF63704.1 AC051625 Genomic DNA No translation available. CP002688 Genomic DNA Translation: AED93894.1 AY080788 mRNA Translation: AAL87271.1 AY133809 mRNA Translation: AAM91743.1 AY086100 mRNA Translation: AAM63308.1 |
RefSeqi | NP_198317.1, NM_122856.3 |
3D structure databases
SMRi | Q8RXN3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 18566, 3 interactors |
STRINGi | 3702.AT5G33320.1 |
PTM databases
iPTMneti | Q8RXN3 |
Proteomic databases
PaxDbi | Q8RXN3 |
PRIDEi | Q8RXN3 |
ProteomicsDBi | 226493 |
Genome annotation databases
EnsemblPlantsi | AT5G33320.1; AT5G33320.1; AT5G33320 |
GeneIDi | 833308 |
Gramenei | AT5G33320.1; AT5G33320.1; AT5G33320 |
KEGGi | ath:AT5G33320 |
Organism-specific databases
Araporti | AT5G33320 |
TAIRi | locus:2145944, AT5G33320 |
Phylogenomic databases
eggNOGi | KOG1441, Eukaryota |
HOGENOMi | CLU_019048_0_0_1 |
InParanoidi | Q8RXN3 |
OMAi | FWYTVSS |
OrthoDBi | 1453018at2759 |
PhylomeDBi | Q8RXN3 |
Miscellaneous databases
PROi | PR:Q8RXN3 |
Gene expression databases
ExpressionAtlasi | Q8RXN3, baseline and differential |
Genevisiblei | Q8RXN3, AT |
Family and domain databases
InterProi | View protein in InterPro IPR004853, Sugar_P_trans_dom IPR004696, Tpt_PEP_transl |
Pfami | View protein in Pfam PF03151, TPT, 1 hit |
TIGRFAMsi | TIGR00817, tpt, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPT1_ARATH | |
Accessioni | Q8RXN3Primary (citable) accession number: Q8RXN3 Secondary accession number(s): P92991, Q8LDB3, Q9M669 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 8, 2011 |
Last sequence update: | June 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families