UniProtKB - Q8RWZ6 (PIN4_ARATH)
Protein
Auxin efflux carrier component 4
Gene
PIN4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Acts as a component of the auxin efflux carrier. Plays a role in generating a sink for auxin into columella cells (PubMed:20439545). Maintains the endogenous auxin gradient, which is essential for correct root patterning (PubMed:20439545). Involved in EXO70A3-regulated gravitropic responses in columella cells and in root system architecture (RSA) (PubMed:31299202).2 Publications
GO - Molecular functioni
- auxin efflux transmembrane transporter activity Source: GO_Central
GO - Biological processi
- auxin-activated signaling pathway Source: UniProtKB-KW
- auxin efflux Source: GO_Central
- auxin homeostasis Source: GO_Central
- auxin polar transport Source: TAIR
- gravitropism Source: UniProtKB
- root development Source: TAIR
- root morphogenesis Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Biological process | Auxin signaling pathway, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Auxin efflux carrier component 41 PublicationShort name: AtPIN41 Publication |
Gene namesi | Name:PIN41 Publication Ordered Locus Names:At2g01420Imported ORF Names:F2I9.4Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G01420 |
TAIRi | locus:2038781, AT2G01420 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein Curated
Note: Distribution in columella cells is regulated by EXO70A3.1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Mitochondrion
- mitochondrion Source: TAIR
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 39 – 59 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 71 – 91 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 102 – 122 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 132 – 152 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 477 – 497 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 501 – 521 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 536 – 556 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 562 – 582 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 596 – 616 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000123783 | 1 – 616 | Auxin efflux carrier component 4Add BLAST | 616 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 223 | PhosphoserineBy similarity | 1 | ||
Glycosylationi | 224 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Modified residuei | 240 | PhosphoserineBy similarity | 1 | ||
Glycosylationi | 243 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Modified residuei | 280 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 358 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 395 | PhosphoserineCombined sources | 1 | ||
Isoform 2 (identifier: Q8RWZ6-2) | |||||
Modified residuei | 395 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q8RWZ6 |
PRIDEi | Q8RWZ6 |
ProteomicsDBi | 235030 [Q8RWZ6-1] |
PTM databases
iPTMneti | Q8RWZ6 |
Expressioni
Tissue specificityi
Expressed in the quiescent center precursors and surrounding cells (PubMed:22540348). Present in columella cells of primary roots (PubMed:31299202). Detected in pollen (PubMed:22540348).2 Publications
Developmental stagei
Expressed during embryogenesis (PubMed:14614497). Detected in the embryonic and seedling root meristems (PubMed:14614497).1 Publication
Inductioni
Down-regulated by endoplasmic reticulum stress treatment.1 Publication
Gene expression databases
ExpressionAtlasi | Q8RWZ6, baseline and differential |
Genevisiblei | Q8RWZ6, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 73, 62 interactors |
IntActi | Q8RWZ6, 59 interactors |
STRINGi | 3702.AT2G01420.2 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QRM7, Eukaryota |
InParanoidi | Q8RWZ6 |
OMAi | KATHMPP |
PhylomeDBi | Q8RWZ6 |
Family and domain databases
InterProi | View protein in InterPro IPR014024, Auxin_eff_plant IPR004776, Mem_trans |
Pfami | View protein in Pfam PF03547, Mem_trans, 1 hit |
TIGRFAMsi | TIGR00946, 2a69, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q8RWZ6-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MITWHDLYTV LTAVVPLYVA MILAYGSVQW WKIFSPDQCS GINRFVAIFA
60 70 80 90 100
VPLLSFHFIS TNDPYAMNFR FVAADTLQKI IMLVLLALWA NLTKNGSLEW
110 120 130 140 150
MITIFSLSTL PNTLVMGIPL LIAMYGTYAG SLMVQVVVLQ CIIWYTLLLF
160 170 180 190 200
LFEYRGAKLL IMEQFPETGA SIVSFKVESD VVSLDGHDFL ETDAEIGNDG
210 220 230 240 250
KLHVTVRKSN ASRRSLMMTP RPSNLTGAEI YSLSSTPRGS NFNHSDFYSV
260 270 280 290 300
MGFPGGRLSN FGPADLYSVQ SSRGPTPRPS NFEENNAVKY GFYNNTNSSV
310 320 330 340 350
PAAGSYPAPN PEFSTGTGVS TKPNKIPKEN QQQLQEKDSK ASHDAKELHM
360 370 380 390 400
FVWSSSASPV SDVFGGGAGD NVATEQSEQG AKEIRMVVSD QPRKSNARGG
410 420 430 440 450
GDDIGGLDSG EGEREIEKAT AGLNKMGSNS TAELEAAGGD GGGNNGTHMP
460 470 480 490 500
PTSVMTRLIL IMVWRKLIRN PNTYSSLIGL IWALVAYRWH VAMPKILQQS
510 520 530 540 550
ISILSDAGLG MAMFSLGLFM ALQPKIIACG NSVATFAMAV RFITGPAIMA
560 570 580 590 600
VAGIAIGLHG DLLRIAIVQA ALPQGIVPFV FAKEYNVHPT ILSTGVIFGM
610
LIALPITLVY YILLGL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8AY30 | A0A1P8AY30_ARATH | Auxin efflux carrier component | PIN4 ARABIDOPSIS PIN-FORMED 4, ATPIN4, PIN-FORMED 4, At2g01420, F10A8.27 | 519 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009419 | 396 – 399 | Missing in isoform 2. 1 Publication | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF087016 mRNA Translation: AAF36769.1 AC005560 Genomic DNA Translation: AAC67319.2 AC006200 Genomic DNA Translation: AAM15143.1 CP002685 Genomic DNA Translation: AEC05448.1 CP002685 Genomic DNA Translation: AEC05449.1 AY091009 mRNA Translation: AAM14031.1 BT008691 mRNA Translation: AAP40497.1 |
PIRi | E84424 |
RefSeqi | NP_565261.1, NM_126203.3 [Q8RWZ6-2] NP_849923.1, NM_179592.2 [Q8RWZ6-1] |
Genome annotation databases
EnsemblPlantsi | AT2G01420.1; AT2G01420.1; AT2G01420 [Q8RWZ6-2] AT2G01420.2; AT2G01420.2; AT2G01420 [Q8RWZ6-1] |
GeneIDi | 814670 |
Gramenei | AT2G01420.1; AT2G01420.1; AT2G01420 [Q8RWZ6-2] AT2G01420.2; AT2G01420.2; AT2G01420 [Q8RWZ6-1] |
KEGGi | ath:AT2G01420 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF087016 mRNA Translation: AAF36769.1 AC005560 Genomic DNA Translation: AAC67319.2 AC006200 Genomic DNA Translation: AAM15143.1 CP002685 Genomic DNA Translation: AEC05448.1 CP002685 Genomic DNA Translation: AEC05449.1 AY091009 mRNA Translation: AAM14031.1 BT008691 mRNA Translation: AAP40497.1 |
PIRi | E84424 |
RefSeqi | NP_565261.1, NM_126203.3 [Q8RWZ6-2] NP_849923.1, NM_179592.2 [Q8RWZ6-1] |
3D structure databases
SMRi | Q8RWZ6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 73, 62 interactors |
IntActi | Q8RWZ6, 59 interactors |
STRINGi | 3702.AT2G01420.2 |
PTM databases
iPTMneti | Q8RWZ6 |
Proteomic databases
PaxDbi | Q8RWZ6 |
PRIDEi | Q8RWZ6 |
ProteomicsDBi | 235030 [Q8RWZ6-1] |
Genome annotation databases
EnsemblPlantsi | AT2G01420.1; AT2G01420.1; AT2G01420 [Q8RWZ6-2] AT2G01420.2; AT2G01420.2; AT2G01420 [Q8RWZ6-1] |
GeneIDi | 814670 |
Gramenei | AT2G01420.1; AT2G01420.1; AT2G01420 [Q8RWZ6-2] AT2G01420.2; AT2G01420.2; AT2G01420 [Q8RWZ6-1] |
KEGGi | ath:AT2G01420 |
Organism-specific databases
Araporti | AT2G01420 |
TAIRi | locus:2038781, AT2G01420 |
Phylogenomic databases
eggNOGi | ENOG502QRM7, Eukaryota |
InParanoidi | Q8RWZ6 |
OMAi | KATHMPP |
PhylomeDBi | Q8RWZ6 |
Miscellaneous databases
PROi | PR:Q8RWZ6 |
Gene expression databases
ExpressionAtlasi | Q8RWZ6, baseline and differential |
Genevisiblei | Q8RWZ6, AT |
Family and domain databases
InterProi | View protein in InterPro IPR014024, Auxin_eff_plant IPR004776, Mem_trans |
Pfami | View protein in Pfam PF03547, Mem_trans, 1 hit |
TIGRFAMsi | TIGR00946, 2a69, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIN4_ARATH | |
Accessioni | Q8RWZ6Primary (citable) accession number: Q8RWZ6 Secondary accession number(s): Q9M7Q6, Q9MAS3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 16, 2004 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families