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UniProtKB - Q8RWS6 (FRO6_ARATH)
Protein
Ferric reduction oxidase 6
Gene
FRO6
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Ferric chelate reductase involved in iron uptake by shoot and leaf cells. May participate in the transport of electrons to a Fe3+ ion via FAD and heme intermediates.
3 PublicationsCatalytic activityi
- EC:1.16.1.7
Cofactori
FADCurated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 214 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 228 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 289 | Iron (heme axial ligand)Curated | 1 | |
Metal bindingi | 302 | Iron (heme axial ligand)Curated | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 377 – 380 | FADSequence analysis | 4 | |
Nucleotide bindingi | 437 – 440 | NADSequence analysis | 4 |
GO - Molecular functioni
- ferric-chelate reductase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- ion transport Source: UniProtKB-KW
- response to light stimulus Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Ion transport, Transport |
Ligand | FAD, Flavoprotein, Heme, Iron, Metal-binding, NAD |
Names & Taxonomyi
Protein namesi | Recommended name: Ferric reduction oxidase 6 (EC:1.16.1.7)Short name: AtFRO6 Alternative name(s): Ferric-chelate reductase 6 |
Gene namesi | Name:FRO6 Ordered Locus Names:At5g49730 ORF Names:K2I5.9 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G49730 |
TAIRi | locus:2157027, AT5G49730 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
- plastid Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 29 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 30 – 49 | HelicalBy similarityAdd BLAST | 20 | |
Topological domaini | 50 – 71 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 72 – 90 | HelicalBy similarityAdd BLAST | 19 | |
Topological domaini | 91 – 122 | CytoplasmicSequence analysisAdd BLAST | 32 | |
Transmembranei | 123 – 146 | HelicalBy similarityAdd BLAST | 24 | |
Topological domaini | 147 – 212 | ExtracellularSequence analysisAdd BLAST | 66 | |
Transmembranei | 213 – 236 | HelicalBy similarityAdd BLAST | 24 | |
Topological domaini | 237 – 287 | CytoplasmicSequence analysisAdd BLAST | 51 | |
Transmembranei | 288 – 312 | HelicalBy similarityAdd BLAST | 25 | |
Topological domaini | 313 – 334 | ExtracellularSequence analysisAdd BLAST | 22 | |
Transmembranei | 335 – 355 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 356 – 564 | CytoplasmicSequence analysisAdd BLAST | 209 | |
Transmembranei | 565 – 587 | HelicalBy similarityAdd BLAST | 23 | |
Topological domaini | 588 – 602 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 603 – 625 | HelicalBy similarityAdd BLAST | 23 | |
Topological domaini | 626 – 738 | CytoplasmicSequence analysisAdd BLAST | 113 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000413204 | 1 – 738 | Ferric reduction oxidase 6Add BLAST | 738 |
Proteomic databases
PaxDbi | Q8RWS6 |
ProteomicsDBi | 230562 |
Expressioni
Tissue specificityi
Expressed in shoots and leaves. Detected in cotyledons, stems, siliques, flowers, sepals, anther filaments and stigmas, but not in roots.2 Publications
Developmental stagei
Not expressed in germinating seeds. Expressed during cell or organ differentiation.1 Publication
Inductioni
Down-regulated in shoots by copper deficiency. Not regulated by iron availability. Light dependent expression.2 Publications
Gene expression databases
ExpressionAtlasi | Q8RWS6, baseline and differential |
Genevisiblei | Q8RWS6, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 178 – 299 | Ferric oxidoreductaseAdd BLAST | 122 | |
Domaini | 328 – 445 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 118 |
Sequence similaritiesi
Belongs to the ferric reductase (FRE) family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
HOGENOMi | CLU_014777_0_0_1 |
InParanoidi | Q8RWS6 |
OrthoDBi | 190356at2759 |
PhylomeDBi | Q8RWS6 |
Family and domain databases
Gene3Di | 3.40.50.80, 2 hits |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8RWS6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDDYETPLLS KDSSSSSSVI TSSLKWILKV VMSMIFVTWV VFLMMMYPEQ
60 70 80 90 100
LGDDFLTFVS SKTFLGTTGT MFLIFSCPIL VISVLASLYL IISGEDKVFT
110 120 130 140 150
KKKISKFPRF RLWTFPVLVD GPFGVVSAAE FLGIMVFSVF FLWSIYAYTL
160 170 180 190 200
RNLDLLERFH VLPKNRSILL LEVTGLRLGM IGLLCMVFLF LPISRGSILL
210 220 230 240 250
RLIDIPFEHA TRYHVWLGHI TMAFFSLHGL CYVVGWIIQG QLLELIFSWN
260 270 280 290 300
AIGIAILPGV ISLVAGLLMW VTSLHTVRKN YFELFFYTHQ LYIVFIVFLA
310 320 330 340 350
LHVGDYMFSI VAGGIFLFIL DRFLRFCQSR RTVDVISAKS LPCGTLELVL
360 370 380 390 400
SKPPNMRYNA LSFIFLQVRE LSWLQWHPFS VSSSPLDGNH HVAVLIKVLG
410 420 430 440 450
GWTAKLRDQL SNLYEAENQD QLISPQSYPK ITTCVEGPYG HESPYHLAYE
460 470 480 490 500
NLVLVAGGIG ITPFFAILSD ILHRKRDGKA CLPSKVLVVW AIKNSDELSL
510 520 530 540 550
LSAIDIPSIC PFFSKKLNLE IHIYITRQSE PRLEDGMVHK VVHPSVKLPR
560 570 580 590 600
TNGCSMSVLV GTGDNIWSGL YLIISTIGFI SMITLLDIFY IKRYNITTWW
610 620 630 640 650
YKGLLFVGCM VASVLIFGGL VVVFWHRWEH KTGEVEANGN DKVDLNGEET
660 670 680 690 700
HNPSAAELKG LAIEEDVQNY TTIRYGTRPA FREIFESLNG KWGSVDVGVI
710 720 730
VCGPATLQTT VAKEIRSHSI WRSANHPLFH FNSHSFDL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8BH71 | A0A1P8BH71_ARATH | Ferric reduction oxidase 6 | FRO6 ATFRO6, ferric reduction oxidase 6, At5g49730, K2I5.9, K2I5_9 | 537 | Annotation score: |
Sequence cautioni
The sequence BAA98161 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB025613 Genomic DNA Translation: BAA98161.1 Sequence problems. CP002688 Genomic DNA Translation: AED95851.1 AY091140 mRNA Translation: AAM14089.1 AY114002 mRNA Translation: AAM45050.1 AK230058 mRNA Translation: BAF01880.1 |
RefSeqi | NP_199784.2, NM_124351.4 |
Genome annotation databases
EnsemblPlantsi | AT5G49730.1; AT5G49730.1; AT5G49730 |
GeneIDi | 835036 |
Gramenei | AT5G49730.1; AT5G49730.1; AT5G49730 |
KEGGi | ath:AT5G49730 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB025613 Genomic DNA Translation: BAA98161.1 Sequence problems. CP002688 Genomic DNA Translation: AED95851.1 AY091140 mRNA Translation: AAM14089.1 AY114002 mRNA Translation: AAM45050.1 AK230058 mRNA Translation: BAF01880.1 |
RefSeqi | NP_199784.2, NM_124351.4 |
3D structure databases
AlphaFoldDBi | Q8RWS6 |
SMRi | Q8RWS6 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G49730.1 |
Proteomic databases
PaxDbi | Q8RWS6 |
ProteomicsDBi | 230562 |
Genome annotation databases
EnsemblPlantsi | AT5G49730.1; AT5G49730.1; AT5G49730 |
GeneIDi | 835036 |
Gramenei | AT5G49730.1; AT5G49730.1; AT5G49730 |
KEGGi | ath:AT5G49730 |
Organism-specific databases
Araporti | AT5G49730 |
TAIRi | locus:2157027, AT5G49730 |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
HOGENOMi | CLU_014777_0_0_1 |
InParanoidi | Q8RWS6 |
OrthoDBi | 190356at2759 |
PhylomeDBi | Q8RWS6 |
Miscellaneous databases
PROi | PR:Q8RWS6 |
Gene expression databases
ExpressionAtlasi | Q8RWS6, baseline and differential |
Genevisiblei | Q8RWS6, AT |
Family and domain databases
Gene3Di | 3.40.50.80, 2 hits |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | FRO6_ARATH | |
Accessioni | Q8RWS6Primary (citable) accession number: Q8RWS6 Secondary accession number(s): Q0WLX7, Q9LTA0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2011 |
Last sequence update: | June 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families