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Protein

Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial

Gene

At3g13930

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).1 Publication

Catalytic activityi

Acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine = CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine.

Cofactori

(R)-lipoateNote: Binds 1 lipoyl cofactor covalently.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei512Sequence analysis1
Active sitei516Sequence analysis1

GO - Molecular functioni

  • copper ion binding Source: TAIR
  • dihydrolipoyllysine-residue acetyltransferase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processGlycolysis

Enzyme and pathway databases

BioCyciARA:AT3G13930-MONOMER
ReactomeiR-ATH-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-ATH-5362517 Signaling by Retinoic Acid
R-ATH-70268 Pyruvate metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrial (EC:2.3.1.12)
Alternative name(s):
Dihydrolipoamide S-acetyltransferase component 2 of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2 2
Short name:
PDC-E2 2
Short name:
PDCE2 2
Gene namesi
Ordered Locus Names:At3g13930
ORF Names:MDC16.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G13930
TAIRilocus:2088247 AT3G13930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 102Mitochondrion1 PublicationAdd BLAST102
ChainiPRO_0000260026103 – 539Dihydrolipoyllysine-residue acetyltransferase component 2 of pyruvate dehydrogenase complex, mitochondrialAdd BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

PaxDbiQ8RWN9
PRIDEiQ8RWN9

PTM databases

iPTMnetiQ8RWN9

Expressioni

Gene expression databases

ExpressionAtlasiQ8RWN9 baseline and differential
GenevisibleiQ8RWN9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G13930.1

Structurei

3D structure databases

ProteinModelPortaliQ8RWN9
SMRiQ8RWN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 187Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST77
Domaini248 – 285Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST38

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Curated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG0557 Eukaryota
COG0508 LUCA
HOGENOMiHOG000281566
InParanoidiQ8RWN9
KOiK00627
OMAiSWRLGCD
OrthoDBiEOG093607WT
PhylomeDBiQ8RWN9

Family and domain databases

Gene3Di3.30.559.10, 1 hit
4.10.320.10, 1 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR001078 2-oxoacid_DH_actylTfrase
IPR000089 Biotin_lipoyl
IPR023213 CAT-like_dom_sf
IPR036625 E3-bd_dom_sf
IPR006257 LAT1
IPR004167 PSBD
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF00198 2-oxoacid_dh, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02817 E3_binding, 1 hit
SUPFAMiSSF47005 SSF47005, 1 hit
SSF51230 SSF51230, 1 hit
TIGRFAMsiTIGR01349 PDHac_trf_mito, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00189 LIPOYL, 1 hit
PS51826 PSBD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8RWN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRIINHSK KLKHVSALLR RDHAVAVRCF SNSTHPSLVG REDIFKARLN
60 70 80 90 100
YSSVERISKC GTGNVTMLSG ISTTSTKLSS PMAGPKLFKE FISSQMRSVR
110 120 130 140 150
GFSSSSDLPP HQEIGMPSLS PTMTEGNIAR WLKKEGDKVA PGEVLCEVET
160 170 180 190 200
DKATVEMECM EEGFLAKIVK EEGAKEIQVG EVIAITVEDE DDIQKFKDYT
210 220 230 240 250
PSSDTGPAAP EAKPAPSLPK EEKVEKPASA PEAKISKPSS APSEDRIFAS
260 270 280 290 300
PLARKLAEDN NVPLSSIKGT GPEGRIVKAD VEDFLASGSK ETTAKPSKQV
310 320 330 340 350
DSKVPALDYV DIPHTQIRKV TASRLAFSKQ TIPHYYLTVD TCVDKMMGLR
360 370 380 390 400
SQLNSFQEAS GGKRISVNDL VIKAAALALR KVPQCNSSWT DEYIRQFKNV
410 420 430 440 450
NINVAVQTEN GLYVPVVKDA DKKGLSTIGE EVRFLAQKAK ENSLKPEDYE
460 470 480 490 500
GGTFTVSNLG GPFGIKQFCA VINPPQAAIL AIGSAEKRVV PGTGPDQYNV
510 520 530
ASYMSVTLSC DHRVIDGAIG AEWLKAFKGY IETPESMLL
Length:539
Mass (Da):58,468
Last modified:November 28, 2006 - v2
Checksum:i219B4BE0E7ACD05A
GO

Sequence cautioni

The sequence BAB02323 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124T → A in AAM12967 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019229 Genomic DNA Translation: BAB02323.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE75442.1
AF367302 mRNA Translation: AAK32889.1
AY091691 mRNA Translation: AAM10290.1
AY092968 mRNA Translation: AAM12967.1
BT000444 mRNA Translation: AAN17421.1
BT000702 mRNA Translation: AAN31846.1
BT001223 mRNA Translation: AAN65110.1
RefSeqiNP_566470.1, NM_112247.3
UniGeneiAt.21338

Genome annotation databases

EnsemblPlantsiAT3G13930.1; AT3G13930.1; AT3G13930
GeneIDi820606
GrameneiAT3G13930.1; AT3G13930.1; AT3G13930
KEGGiath:AT3G13930

Similar proteinsi

Entry informationi

Entry nameiODP22_ARATH
AccessioniPrimary (citable) accession number: Q8RWN9
Secondary accession number(s): Q9ASS8, Q9LVK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: April 25, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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