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UniProtKB - Q8RWN2 (ZIF1_ARATH)
Protein
Protein ZINC INDUCED FACILITATOR 1
Gene
ZIF1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Major facilitator superfamily (MFS) transporter involved in zinc tolerance by participating in vacuolar sequestration of zinc.
1 PublicationMiscellaneous
Overexpression of ZIF1 confers increased zinc tolerance and causes interveinal leaf chlorosis.
GO - Molecular functioni
- potassium ion antiporter activity Source: GO_Central
GO - Biological processi
- regulation of stomatal closure Source: GO_Central
- response to nematode Source: TAIR
- response to zinc ion Source: TAIR
- zinc ion homeostasis Source: TAIR
Keywordsi
Biological process | Transport |
Protein family/group databases
TCDBi | 2.A.1.2.58, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Protein ZINC INDUCED FACILITATOR 1 |
Gene namesi | Name:ZIF1 Ordered Locus Names:At5g13740 ORF Names:MSH12.21 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G13740 |
TAIRi | locus:2173254, AT5G13740 |
Subcellular locationi
Vacuole
- Vacuole membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: GO_Central
Vacuole
- plant-type vacuole membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 41 – 61 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 82 – 102 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 109 – 129 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 131 – 151 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 170 – 190 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 212 – 232 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 288 – 308 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 327 – 347 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 362 – 384 | HelicalSequence analysisAdd BLAST | 23 | |
Transmembranei | 391 – 408 | HelicalSequence analysisAdd BLAST | 18 | |
Transmembranei | 423 – 443 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 461 – 481 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, VacuolePathology & Biotechi
Disruption phenotypei
Zinc sensitivity resulting in increased chlorosis and decreased shoot fresh weight and root length. Accumulation of high levels of zinc in shoots.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 472 | G → R in zif1-3; zinc sensitivity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000400080 | 1 – 486 | Protein ZINC INDUCED FACILITATOR 1Add BLAST | 486 |
Proteomic databases
PaxDbi | Q8RWN2 |
ProteomicsDBi | 232306 |
Expressioni
Tissue specificityi
Strongly expressed in developing leaves, differentiating zones of root tips and sepals of developing flowers. Restricted to vascular tissues in older leaves, mature roots, flowers, anthers and filaments. Not expressed in developing anthers.1 Publication
Inductioni
Up-regulated by zinc and manganese. Not induced by cadmium, copper or iron. Down-regulated upon nematode infection.2 Publications
Gene expression databases
ExpressionAtlasi | Q8RWN2, baseline and differential |
Genevisiblei | Q8RWN2, AT |
Structurei
Family & Domainsi
Sequence similaritiesi
Belongs to the major facilitator superfamily.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2615, Eukaryota |
HOGENOMi | CLU_001265_54_2_1 |
InParanoidi | Q8RWN2 |
OMAi | IYPFAPQ |
OrthoDBi | 1090653at2759 |
PhylomeDBi | Q8RWN2 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR001958, Tet-R_TetA/multi-R_MdtG |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
PRINTSi | PR01035, TCRTETA |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q8RWN2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEEYKEALL EKQNYHDGCP GCKVEQMKQL RRGYPYLELS FVWIIVLSTS
60 70 80 90 100
LPISSLYPFL YYMIEDFGVA KTEKDIGFYA GFVGCSFMLG RALTSVFWGI
110 120 130 140 150
VADRYGRKPI ILLGTISIAI FNALFGLSSN FWMAIGTRFL LGSFNCLLGT
160 170 180 190 200
MKAYASEIFR DEYQATAMSA VSTAWGIGLI IGPALGGFLA QPADKYPNVF
210 220 230 240 250
SQESLFGRFR YALPCFTISA FALLVTVLCC FIPETLHNHK LDSLSHDDSY
260 270 280 290 300
DILEAASHES SPSTGKAGKN ERKASQSLLK NWPLMSSIIV YCVLCLHDTA
310 320 330 340 350
YSEIFALWAN SPRKYGGLSY STNEVGTVLA ISGLGLFSFQ VFVYPLAEKL
360 370 380 390 400
LGPVLVTRYA GALMIPIQMS YPFIAGLSGL SLSLMLNCAS ILINVLSVSA
410 420 430 440 450
ITGLLILQNR AVDQSQRGAA NGIAMTAMSL FKTVGPAGAG ILFSWSERRL
460 470 480
NAAFLPGSHM VFFVLNVIVV VGVALTFKPF LTTSRR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8BEY5 | A0A1P8BEY5_ARATH | Zinc induced facilitator 1 | ZIF1 MSH12.21, MSH12_21, zinc induced facilitator 1, At5g13740 | 442 | Annotation score: |
Sequence cautioni
The sequence AAM12978 differs from that shown. Reason: Frameshift.Curated
The sequence BAB10595 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB011484, AB006704 Genomic DNA Translation: BAB10595.1 Sequence problems. CP002688 Genomic DNA Translation: AED91934.1 AY092979 mRNA Translation: AAM12978.1 Frameshift. AY128800 mRNA Translation: AAM91200.1 |
RefSeqi | NP_196878.2, NM_121377.5 |
Genome annotation databases
EnsemblPlantsi | AT5G13740.1; AT5G13740.1; AT5G13740 |
GeneIDi | 831219 |
Gramenei | AT5G13740.1; AT5G13740.1; AT5G13740 |
KEGGi | ath:AT5G13740 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB011484, AB006704 Genomic DNA Translation: BAB10595.1 Sequence problems. CP002688 Genomic DNA Translation: AED91934.1 AY092979 mRNA Translation: AAM12978.1 Frameshift. AY128800 mRNA Translation: AAM91200.1 |
RefSeqi | NP_196878.2, NM_121377.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 3702.AT5G13740.1 |
Protein family/group databases
TCDBi | 2.A.1.2.58, the major facilitator superfamily (mfs) |
Proteomic databases
PaxDbi | Q8RWN2 |
ProteomicsDBi | 232306 |
Genome annotation databases
EnsemblPlantsi | AT5G13740.1; AT5G13740.1; AT5G13740 |
GeneIDi | 831219 |
Gramenei | AT5G13740.1; AT5G13740.1; AT5G13740 |
KEGGi | ath:AT5G13740 |
Organism-specific databases
Araporti | AT5G13740 |
TAIRi | locus:2173254, AT5G13740 |
Phylogenomic databases
eggNOGi | KOG2615, Eukaryota |
HOGENOMi | CLU_001265_54_2_1 |
InParanoidi | Q8RWN2 |
OMAi | IYPFAPQ |
OrthoDBi | 1090653at2759 |
PhylomeDBi | Q8RWN2 |
Miscellaneous databases
PROi | PR:Q8RWN2 |
Gene expression databases
ExpressionAtlasi | Q8RWN2, baseline and differential |
Genevisiblei | Q8RWN2, AT |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR001958, Tet-R_TetA/multi-R_MdtG |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
PRINTSi | PR01035, TCRTETA |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ZIF1_ARATH | |
Accessioni | Q8RWN2Primary (citable) accession number: Q8RWN2 Secondary accession number(s): Q9FKI8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 2, 2010 |
Last sequence update: | November 2, 2010 | |
Last modified: | February 23, 2022 | |
This is version 109 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families