UniProtKB - Q8RUW5 (SCP8_ARATH)
Serine carboxypeptidase-like 8
SCPL8
Functioni
Involved in plants secondary metabolism. Functions as acyltransferase to form the sinapate ester sinapoylmalate. Also capable of catalyzing the formation of 1,2-bis-O-sinapoyl beta-D-glucoside.
5 PublicationsMiscellaneous
Caution
Catalytic activityi
- EC:2.3.1.923 PublicationsThis reaction proceeds in the forward3 Publications direction.
- EC:2.3.1.1033 PublicationsThis reaction proceeds in the forward3 Publications direction.
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 173 | By similarity | 1 | |
Active sitei | 358 | By similarity | 1 | |
Active sitei | 411 | By similarity | 1 |
GO - Molecular functioni
- acyltransferase activity, transferring groups other than amino-acyl groups Source: GO_Central
- serine-type carboxypeptidase activity Source: InterPro
- sinapoylglucose-malate O-sinapoyltransferase activity Source: TAIR
- sinapoylglucose-sinapoylglucose O-sinapoyltransferase activity Source: UniProtKB-EC
GO - Biological processi
- phenylpropanoid metabolic process Source: TAIR
- secondary metabolic process Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | ARA:AT2G22990-MONOMER |
BRENDAi | 2.3.1.92, 399 |
Protein family/group databases
ESTHERi | arath-SCP8, Carboxypeptidase_S10 |
MEROPSi | S10.A14 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine carboxypeptidase-like 81 PublicationAlternative name(s): |
Gene namesi | Name:SCPL81 Publication Synonyms:SMT1 Publication, SNG11 Publication Ordered Locus Names:At2g22990Imported ORF Names:F21P24.5Imported, T20K9.18Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G22990 |
TAIRi | locus:2045374, AT2G22990 |
Subcellular locationi
Vacuole
- Vacuole 1 Publication
Vacuole
- vacuole Source: TAIR
Other locations
- secretory vesicle Source: TAIR
Keywords - Cellular componenti
VacuolePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 73 | N → A: 87% reduction of activity. 1 Publication | 1 | |
Mutagenesisi | 172 – 175 | DSYS → ESYA: 85% reduction of activity. 1 Publication | 4 | |
Mutagenesisi | 173 | S → A: Total loss of activity. 1 Publication | 1 | |
Mutagenesisi | 268 | K → E: 25% reduction of activity. 1 Publication | 1 | |
Mutagenesisi | 272 | H → D: 78% reduction of activity. 1 Publication | 1 | |
Mutagenesisi | 322 | R → E: 99% reduction of activity. 1 Publication | 1 | |
Mutagenesisi | 358 | D → A: 80% reduction of activity. 1 Publication | 1 | |
Mutagenesisi | 411 | H → A: Total loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | 2 PublicationsAdd BLAST | 19 | |
ChainiPRO_0000274622 | 20 – 433 | Serine carboxypeptidase-like 8Add BLAST | 414 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 78 ↔ 323 | By similarity | ||
Glycosylationi | 99 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 241 ↔ 255 | By similarity | ||
Disulfide bondi | 279 ↔ 289 | By similarity | ||
Glycosylationi | 283 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 324 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 342 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 418 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q8RUW5 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | Q8RUW5, baseline and differential |
Genevisiblei | Q8RUW5, AT |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1282, Eukaryota |
HOGENOMi | CLU_008523_0_1_1 |
InParanoidi | Q8RUW5 |
OrthoDBi | 607679at2759 |
PhylomeDBi | Q8RUW5 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR001563, Peptidase_S10 |
PANTHERi | PTHR11802, PTHR11802, 1 hit |
Pfami | View protein in Pfam PF00450, Peptidase_S10, 1 hit |
PRINTSi | PR00724, CRBOXYPTASEC |
SUPFAMi | SSF53474, SSF53474, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLKIKFLLL LVLYHHVDSA SIVKFLPGFE GPLPFELETG YIGIGEDENV
60 70 80 90 100
QFFYYFIKSE NNPKEDPLLI WLNGGPGCSC LGGIIFENGP VGLKFEVFNG
110 120 130 140 150
SAPSLFSTTY SWTKMANIIF LDQPVGSGFS YSKTPIDKTG DISEVKRTHE
160 170 180 190 200
FLQKWLSRHP QYFSNPLYVV GDSYSGMIVP ALVQEISQGN YICCEPPINL
210 220 230 240 250
QGYMLGNPVT YMDFEQNFRI PYAYGMGLIS DEIYEPMKRI CNGNYYNVDP
260 270 280 290 300
SNTQCLKLTE EYHKCTAKIN IHHILTPDCD VTNVTSPDCY YYPYHLIECW
310 320 330 340 350
ANDESVREAL HIEKGSKGKW ARCNRTIPYN HDIVSSIPYH MNNSISGYRS
360 370 380 390 400
LIYSGDHDIA VPFLATQAWI RSLNYSPIHN WRPWMINNQI AGYTRAYSNK
410 420 430
MTFATIKGGG HTAEYRPNET FIMFQRWISG QPL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8MQW0 | A8MQW0_ARATH | Sinapoylglucose 1 | SNG1 MALATE SINAPOYLTRANSFERASE, SCPL8, SERINE CARBOXYPEPTIDASE-LIKE 8, sinapoylglucose 1, At2g22990 | 424 | Annotation score: | ||
A8MR78 | A8MR78_ARATH | Sinapoylglucose 1 | SNG1 MALATE SINAPOYLTRANSFERASE, SCPL8, SERINE CARBOXYPEPTIDASE-LIKE 8, sinapoylglucose 1, At2g22990 | 416 | Annotation score: | ||
F4IKK4 | F4IKK4_ARATH | Sinapoylglucose 1 | SNG1 MALATE SINAPOYLTRANSFERASE, SCPL8, SERINE CARBOXYPEPTIDASE-LIKE 8, sinapoylglucose 1, At2g22990 | 319 | Annotation score: | ||
Q3EBW0 | Q3EBW0_ARATH | Sinapoylglucose 1 | SNG1 MALATE SINAPOYLTRANSFERASE, SCPL8, SERINE CARBOXYPEPTIDASE-LIKE 8, sinapoylglucose 1, At2g22990 | 319 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_027464 | 408 – 433 | GGGHT…SGQPL → ASVDTRQSIDQTRPLSCSKG GSVANPCNKRLMTFTYNYLP TNIHVKRSCLC in isoform 2. CuratedAdd BLAST | 26 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF275313 mRNA Translation: AAF78760.1 AC004401 Genomic DNA Translation: AAC17816.2 Sequence problems. AC004786 Genomic DNA Translation: AAM15006.1 Sequence problems. CP002685 Genomic DNA Translation: AEC07390.1 CP002685 Genomic DNA Translation: AEC07393.1 AF361601 mRNA Translation: AAK32769.1 Frameshift. AY035052 mRNA Translation: AAK59557.1 AY051060 mRNA Translation: AAK93737.1 AY143880 mRNA Translation: AAN28819.1 Sequence problems. |
PIRi | C84619 |
RefSeqi | NP_850034.1, NM_179703.2 [Q8RUW5-1] NP_850035.1, NM_179704.1 [Q8RUW5-2] |
Genome annotation databases
EnsemblPlantsi | AT2G22990.1; AT2G22990.1; AT2G22990 [Q8RUW5-1] AT2G22990.3; AT2G22990.3; AT2G22990 [Q8RUW5-2] |
GeneIDi | 816830 |
Gramenei | AT2G22990.1; AT2G22990.1; AT2G22990 [Q8RUW5-1] AT2G22990.3; AT2G22990.3; AT2G22990 [Q8RUW5-2] |
KEGGi | ath:AT2G22990 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF275313 mRNA Translation: AAF78760.1 AC004401 Genomic DNA Translation: AAC17816.2 Sequence problems. AC004786 Genomic DNA Translation: AAM15006.1 Sequence problems. CP002685 Genomic DNA Translation: AEC07390.1 CP002685 Genomic DNA Translation: AEC07393.1 AF361601 mRNA Translation: AAK32769.1 Frameshift. AY035052 mRNA Translation: AAK59557.1 AY051060 mRNA Translation: AAK93737.1 AY143880 mRNA Translation: AAN28819.1 Sequence problems. |
PIRi | C84619 |
RefSeqi | NP_850034.1, NM_179703.2 [Q8RUW5-1] NP_850035.1, NM_179704.1 [Q8RUW5-2] |
3D structure databases
AlphaFoldDBi | Q8RUW5 |
SMRi | Q8RUW5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G22990.3 |
Protein family/group databases
ESTHERi | arath-SCP8, Carboxypeptidase_S10 |
MEROPSi | S10.A14 |
Proteomic databases
PaxDbi | Q8RUW5 |
Genome annotation databases
EnsemblPlantsi | AT2G22990.1; AT2G22990.1; AT2G22990 [Q8RUW5-1] AT2G22990.3; AT2G22990.3; AT2G22990 [Q8RUW5-2] |
GeneIDi | 816830 |
Gramenei | AT2G22990.1; AT2G22990.1; AT2G22990 [Q8RUW5-1] AT2G22990.3; AT2G22990.3; AT2G22990 [Q8RUW5-2] |
KEGGi | ath:AT2G22990 |
Organism-specific databases
Araporti | AT2G22990 |
TAIRi | locus:2045374, AT2G22990 |
Phylogenomic databases
eggNOGi | KOG1282, Eukaryota |
HOGENOMi | CLU_008523_0_1_1 |
InParanoidi | Q8RUW5 |
OrthoDBi | 607679at2759 |
PhylomeDBi | Q8RUW5 |
Enzyme and pathway databases
BioCyci | ARA:AT2G22990-MONOMER |
BRENDAi | 2.3.1.92, 399 |
Miscellaneous databases
PROi | PR:Q8RUW5 |
Gene expression databases
ExpressionAtlasi | Q8RUW5, baseline and differential |
Genevisiblei | Q8RUW5, AT |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR001563, Peptidase_S10 |
PANTHERi | PTHR11802, PTHR11802, 1 hit |
Pfami | View protein in Pfam PF00450, Peptidase_S10, 1 hit |
PRINTSi | PR00724, CRBOXYPTASEC |
SUPFAMi | SSF53474, SSF53474, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SCP8_ARATH | |
Accessioni | Q8RUW5Primary (citable) accession number: Q8RUW5 Secondary accession number(s): O64809, Q3EBV9, Q9ASY4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2007 |
Last sequence update: | February 6, 2007 | |
Last modified: | May 25, 2022 | |
This is version 125 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families