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Protein

Acetone carboxylase beta subunit

Gene

acxA

Organism
Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the carboxylation of acetone to form acetoacetate. Has a reduced activity on butanone, and no activity on 2-pentatone, 3-pentatone, 2-hexanone, chloroacetone, pyruvate, phosphoenolpyruvate, acetaldehyde, propionaldehyde and propylene oxide.1 Publication

Catalytic activityi

Acetone + CO2 + ATP + 2 H2O = acetoacetate + AMP + 2 phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:

Kineticsi

  1. KM=7.80 µM for acetone (in the presence of CO2)1 Publication
  2. KM=7.68 µM for acetone (in the absence of CO2)1 Publication
  3. KM=0.122 mM for ATP1 Publication
  4. KM=4.17 mM for CO21 Publication
  1. Vmax=0.485 µmol/min/mg enzyme toward acetone (in the presence of CO2)1 Publication
  2. Vmax=0.616 µmol/min/mg enzyme toward acetone (in the absence of CO2)1 Publication
  3. Vmax=0.463 µmol/min/mg enzyme toward ATP1 Publication
  4. Vmax=0.225 µmol/min/mg enzyme toward CO21 Publication

pH dependencei

Optimum pH is 7.6.1 Publication

GO - Molecular functioni

  • acetone carboxylase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • hydrolase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13281

Names & Taxonomyi

Protein namesi
Recommended name:
Acetone carboxylase beta subunitImported (EC:6.4.1.6)
Gene namesi
Name:acxAImported
Ordered Locus Names:Xaut_3509
OrganismiXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Taxonomic identifieri78245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter
Proteomesi
  • UP000002417 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004030511 – 717Acetone carboxylase beta subunitAdd BLAST717

Post-translational modificationi

The N-terminus is blocked.1 Publication

Proteomic databases

PRIDEiQ8RM04

Interactioni

Subunit structurei

Heterohexamer of two alpha, two beta and two gamma subunits.1 Publication

Protein-protein interaction databases

STRINGi78245.Xaut_3509

Structurei

Secondary structure

1717
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiQ8RM04
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the oxoprolinase family.Curated

Phylogenomic databases

eggNOGiENOG4107RID Bacteria
COG0145 LUCA
HOGENOMiHOG000146376
KOiK10855
OMAiIHLNTHR
OrthoDBiPOG091H0EVX

Family and domain databases

InterProiView protein in InterPro
IPR008040 Hydant_A_N
IPR002821 Hydantoinase_A
PfamiView protein in Pfam
PF05378 Hydant_A_N, 1 hit
PF01968 Hydantoinase_A, 1 hit

Sequencei

Sequence statusi: Complete.

Q8RM04-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNVPVGHLRN VQVLGIDAGG TMTDTFFVDQ DGDFVVGKAQ STPQNEALGL
60 70 80 90 100
IASSEDGLAN WGMSLHEALA QLQTGVYSGT AMLNRVVQRK GLKCGLIVNR
110 120 130 140 150
GMEDFHRMGR AVQSHLGYAY EDRIHLNTHR YDPPLVPRHL TRGVVERTDM
160 170 180 190 200
IGTQVIPLRE DTARDAARDL IAADAEGIVI SLLHSYKNPE NERRVRDIVL
210 220 230 240 250
EEVEKSGKKI PVFASADYYP VRKETHRTNT TILEGYAAEP SRQTLSKISN
260 270 280 290 300
AFKERGTKFD FRVMATHGGT ISWKAKELAR TIVSGPIGGV IGAKYLGEVL
310 320 330 340 350
GYKNIACSDI GGTSFDVALI TQGEMTIKND PDMARLVLSL PLVAMDSVGA
360 370 380 390 400
GAGSFIRLDP YTRAIKLGPD SAGYRVGVCW KESGIETVTI SDCHMVLGYL
410 420 430 440 450
NPDNFLGGAV KLDRQRSVDA IKAQIADPLG LSVEDAAAGV IELLDSDLRD
460 470 480 490 500
YLRSMISGKG YSPASFVCFS YGGAGPVHTY GYTEGLGFED VIVPAWAAGF
510 520 530 540 550
SAFGCAAADF EYRYDKSLDI NMPTETPDTD KEKAAATLQA AWEELTKNVL
560 570 580 590 600
EEFKLNGYSA DQVTLQPGYR MQYRGQLNDL EIESPLAQAH TAADWDQLTD
610 620 630 640 650
AFNATYGRVY AASARSPELG YSVTGAIMRG MVPIPKPKIP KEPEEGETPP
660 670 680 690 700
ESAKIGTRKF YRKKRWVDAQ LYHMESLRPG NRVMGPAVIE SDATTFVVPD
710
GFETWLDGHR LFHLREV
Length:717
Mass (Da):78,509
Last modified:June 1, 2002 - v1
Checksum:iAF30CD9B7078A283
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti352A → R in AAM20736 (PubMed:12003937).Curated1

Mass spectrometryi

Molecular mass is 78300 Da from positions 1 - 717. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF251789 Genomic DNA Translation: AAM20736.1
AY055852 Genomic DNA Translation: AAL17710.1
CP000781 Genomic DNA Translation: ABS68738.1
RefSeqiWP_012115484.1, NC_009720.1

Genome annotation databases

EnsemblBacteriaiABS68738; ABS68738; Xaut_3509
KEGGixau:Xaut_3509

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF251789 Genomic DNA Translation: AAM20736.1
AY055852 Genomic DNA Translation: AAL17710.1
CP000781 Genomic DNA Translation: ABS68738.1
RefSeqiWP_012115484.1, NC_009720.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5M45X-ray1.87B/E/H/K1-717[»]
5SVBX-ray2.65B/E2-717[»]
5SVCX-ray2.70B/E1-717[»]
SMRiQ8RM04
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi78245.Xaut_3509

Proteomic databases

PRIDEiQ8RM04

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS68738; ABS68738; Xaut_3509
KEGGixau:Xaut_3509

Phylogenomic databases

eggNOGiENOG4107RID Bacteria
COG0145 LUCA
HOGENOMiHOG000146376
KOiK10855
OMAiIHLNTHR
OrthoDBiPOG091H0EVX

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13281

Family and domain databases

InterProiView protein in InterPro
IPR008040 Hydant_A_N
IPR002821 Hydantoinase_A
PfamiView protein in Pfam
PF05378 Hydant_A_N, 1 hit
PF01968 Hydantoinase_A, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACXA_XANP2
AccessioniPrimary (citable) accession number: Q8RM04
Secondary accession number(s): Q8L3A7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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