UniProtKB - Q8R5M4 (OPTN_RAT)
Optineurin
Optn
Functioni
Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection. Plays a neuroprotective role in the eye and optic nerve. Probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy) and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52.
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 563 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 566 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 579 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 583 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 555 – 585 | CCHC NOA-typePROSITE-ProRule annotationAdd BLAST | 31 |
GO - Molecular functioni
- identical protein binding Source: RGD
- K63-linked polyubiquitin modification-dependent protein binding Source: RGD
- metal ion binding Source: UniProtKB-KW
- polyubiquitin modification-dependent protein binding Source: RGD
- protein C-terminus binding Source: RGD
- protein-macromolecule adaptor activity Source: RGD
- small GTPase binding Source: RGD
- TFIIIA-class transcription factor binding Source: RGD
- ubiquitin binding Source: RGD
GO - Biological processi
- autophagy Source: UniProtKB-KW
- cellular protein localization Source: RGD
- cellular response to hydrogen peroxide Source: RGD
- cellular response to L-glutamate Source: RGD
- cellular response to nerve growth factor stimulus Source: RGD
- cellular response to tumor necrosis factor Source: RGD
- cellular response to unfolded protein Source: UniProtKB
- defense response to Gram-negative bacterium Source: RGD
- Golgi organization Source: UniProtKB
- Golgi ribbon formation Source: UniProtKB
- Golgi to plasma membrane protein transport Source: RGD
- negative regulation of cell population proliferation Source: RGD
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: RGD
- negative regulation of neuron apoptotic process Source: RGD
- negative regulation of receptor recycling Source: RGD
- neural retina development Source: RGD
- parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization Source: RGD
- positive regulation of autophagy Source: RGD
- positive regulation of cell population proliferation Source: RGD
- positive regulation of cytokine production Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of neural precursor cell proliferation Source: RGD
- positive regulation of neuron apoptotic process Source: RGD
- positive regulation of xenophagy Source: RGD
- protein localization to Golgi apparatus Source: RGD
- regulation of I-kappaB kinase/NF-kappaB signaling Source: GO_Central
- wound healing Source: RGD
Keywordsi
Biological process | Autophagy |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-2565942, Regulation of PLK1 Activity at G2/M Transition R-RNO-8854214, TBC/RABGAPs |
Names & Taxonomyi
Protein namesi | Recommended name: OptineurinAlternative name(s): FIP-2-like protein |
Gene namesi | Name:Optn |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628886, Optn |
Subcellular locationi
Golgi apparatus
- Golgi apparatus By similarity
- trans-Golgi network By similarity
Endosome
- Recycling endosome By similarity
Other locations
- perinuclear region By similarity
- autophagosome By similarity
- Cytoplasmic vesicle By similarity
Note: Found in the perinuclear region and associates with the Golgi apparatus. Colocalizes with MYO6 and RAB8 at the Golgi complex and in vesicular structures close to the plasma membrane. Localizes to LC3-positive cytoplasmic vesicles upon induction of autophagy.By similarity
Endosome
- recycling endosome Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- trans-Golgi network Source: UniProtKB
Nucleus
- nucleus Source: RGD
Other locations
- autophagosome Source: UniProtKB-SubCell
- axon Source: RGD
- cytoplasm Source: RGD
- cytoplasmic vesicle Source: UniProtKB
- neuronal cell body Source: RGD
- perinuclear region of cytoplasm Source: RGD
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, Endosome, Golgi apparatusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058072 | 1 – 585 | OptineurinAdd BLAST | 585 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 188 | PhosphoserineCombined sources | 1 | |
Modified residuei | 209 | PhosphoserineBy similarity | 1 | |
Modified residuei | 346 | PhosphoserineCombined sources | 1 | |
Modified residuei | 531 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8R5M4 |
PRIDEi | Q8R5M4 |
PTM databases
iPTMneti | Q8R5M4 |
PhosphoSitePlusi | Q8R5M4 |
Interactioni
Subunit structurei
Self-associates (By similarity).
Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains.
Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increases the association (at least with MAP1LC3B).
Interacts with RAB12; the interaction may be indirect (By similarity).
Interacts with palmitoyltransferase ZDHHC17/HIP14; the interaction does not lead to palmitoylation of OPTN (By similarity).
Interacts with CYLD (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- K63-linked polyubiquitin modification-dependent protein binding Source: RGD
- polyubiquitin modification-dependent protein binding Source: RGD
- protein C-terminus binding Source: RGD
- small GTPase binding Source: RGD
- TFIIIA-class transcription factor binding Source: RGD
- ubiquitin binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251574, 1 interactor |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 32 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 58 – 220 | Interaction with Rab8By similarityAdd BLAST | 163 | |
Regioni | 200 – 220 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 269 – 299 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 415 – 585 | Interaction with HDBy similarityAdd BLAST | 171 | |
Regioni | 416 – 525 | Interaction with MYO6By similarityAdd BLAST | 110 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 38 – 181 | Sequence analysisAdd BLAST | 144 | |
Coiled coili | 244 – 512 | Sequence analysisAdd BLAST | 269 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 187 – 192 | LIR | 6 | |
Motifi | 478 – 483 | UBAN | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 285 – 299 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 555 – 585 | CCHC NOA-typePROSITE-ProRule annotationAdd BLAST | 31 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QTG2, Eukaryota |
InParanoidi | Q8R5M4 |
OMAi | AQMMTLF |
OrthoDBi | 745047at2759 |
PhylomeDBi | Q8R5M4 |
Family and domain databases
InterProi | View protein in InterPro IPR032419, CC2-LZ_dom IPR021063, NEMO_N IPR034735, NEMO_ZF IPR032939, Optineurin |
PANTHERi | PTHR31553:SF2, PTHR31553:SF2, 1 hit |
Pfami | View protein in Pfam PF16516, CC2-LZ, 1 hit PF11577, NEMO, 1 hit |
PROSITEi | View protein in PROSITE PS51801, ZF_CCHC_NOA, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSHQPLSCLT EKGDSSCETP GNGPSNMVHP NLDTFTPEEL LQQMKELLVE
60 70 80 90 100
NHQLKEAMKL NNQAMKGRFE ELSAWTEKQK EERQLFEIQS KEAKERLKAL
110 120 130 140 150
SHENERLKEE LGKLKEKSER PFEDITGRCG FPRTDLEQEV EQLKRQVEQE
160 170 180 190 200
VEHLKIQVRR LQAEKADLLG IVSELQLKLN SGGSSEDSFV EIRMTEGEAE
210 220 230 240 250
GAMKEMRNSA GPTRTDSISM GKCTEDARTC VEFEELTVSQ LLLCLREGNQ
260 270 280 290 300
KVERLEIALR EAKERISDFE KKANGHSAIE TQTEGSTQKE EEDKDPESVG
310 320 330 340 350
IEVETLNVQV ASLFKELQEA HTKLSEAELM KKRLQEKCQA LERKNSATPS
360 370 380 390 400
ELNEKQELVY SNRKLELQVE SMRSEIKMEQ AKTEEEKSRL ATLQATHDKL
410 420 430 440 450
LQEHNKALRT IEELTKQQAE KVDKVQLQEL SEKLELAEQA LASKQLQMDE
460 470 480 490 500
MKQTIAKQEE DLETMAVLRA QMEVYCSDFH AERAAREKIH EEKEQLALQL
510 520 530 540 550
AILLKENNDF EDGGSRQSLM EMQCRHGART SDSDQQAYLF QRGAEDMSWQ
560 570 580
HGQQPRSIPI HSCPKCGEVL PDIDTLQIHV MDCII
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1M9C4 | F1M9C4_RAT | Optineurin | Optn | 647 | Annotation score: | ||
A0A0G2JUB3 | A0A0G2JUB3_RAT | Optineurin | Optn | 585 | Annotation score: | ||
G8JLS4 | G8JLS4_RAT | Optineurin | Optn | 583 | Annotation score: | ||
A0A0G2JT32 | A0A0G2JT32_RAT | Optineurin | Optn | 509 | Annotation score: | ||
A0A0G2K3Z1 | A0A0G2K3Z1_RAT | Optineurin | Optn | 422 | Annotation score: | ||
M0RAB4 | M0RAB4_RAT | Optineurin | Optn | 355 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 78 | K → R in BAB84696 (Ref. 1) Curated | 1 | |
Sequence conflicti | 172 – 174 | VSE → RLR (Ref. 1) Curated | 3 | |
Sequence conflicti | 219 | S → I in BAB84696 (Ref. 1) Curated | 1 | |
Sequence conflicti | 316 | E → G in BAB84696 (Ref. 1) Curated | 1 | |
Sequence conflicti | 363 | R → W in BAB84696 (Ref. 1) Curated | 1 | |
Sequence conflicti | 545 | E → K in BAB84696 (Ref. 1) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013263 | 156 – 195 | IQVRR…EIRMT → NQ in isoform 2. 1 PublicationAdd BLAST | 40 | |
Alternative sequenceiVSP_013264 | 515 | Missing in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB069907 mRNA Translation: BAB84696.2 Frameshift. BC086976 mRNA Translation: AAH86976.1 |
RefSeqi | NP_659549.2, NM_145081.3 |
Genome annotation databases
GeneIDi | 246294 |
KEGGi | rno:246294 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB069907 mRNA Translation: BAB84696.2 Frameshift. BC086976 mRNA Translation: AAH86976.1 |
RefSeqi | NP_659549.2, NM_145081.3 |
3D structure databases
SMRi | Q8R5M4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 251574, 1 interactor |
PTM databases
iPTMneti | Q8R5M4 |
PhosphoSitePlusi | Q8R5M4 |
Proteomic databases
PaxDbi | Q8R5M4 |
PRIDEi | Q8R5M4 |
Genome annotation databases
GeneIDi | 246294 |
KEGGi | rno:246294 |
Organism-specific databases
CTDi | 10133 |
RGDi | 628886, Optn |
Phylogenomic databases
eggNOGi | ENOG502QTG2, Eukaryota |
InParanoidi | Q8R5M4 |
OMAi | AQMMTLF |
OrthoDBi | 745047at2759 |
PhylomeDBi | Q8R5M4 |
Enzyme and pathway databases
Reactomei | R-RNO-2565942, Regulation of PLK1 Activity at G2/M Transition R-RNO-8854214, TBC/RABGAPs |
Miscellaneous databases
PROi | PR:Q8R5M4 |
Family and domain databases
InterProi | View protein in InterPro IPR032419, CC2-LZ_dom IPR021063, NEMO_N IPR034735, NEMO_ZF IPR032939, Optineurin |
PANTHERi | PTHR31553:SF2, PTHR31553:SF2, 1 hit |
Pfami | View protein in Pfam PF16516, CC2-LZ, 1 hit PF11577, NEMO, 1 hit |
PROSITEi | View protein in PROSITE PS51801, ZF_CCHC_NOA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OPTN_RAT | |
Accessioni | Q8R5M4Primary (citable) accession number: Q8R5M4 Secondary accession number(s): Q5PQX8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | March 29, 2005 | |
Last modified: | February 23, 2022 | |
This is version 117 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |