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Protein

PRA1 family protein 3

Gene

Arl6ip5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates intracellular concentrations of taurine and glutamate (By similarity). Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity-dependent manner (PubMed:12119102, PubMed:18684713). May be involved in membrane traffic (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-210500 Glutamate Neurotransmitter Release Cycle

Names & Taxonomyi

Protein namesi
Recommended name:
PRA1 family protein 3
Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name:
ARL-6-interacting protein 5
Short name:
Aip-5
Addicsin
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
Prenylated Rab acceptor protein 2
Protein JWa
Gene namesi
Name:Arl6ip5
Synonyms:Aip5, Jwa, Pra2, Praf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1929501 Arl6ip5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 39CytoplasmicBy similarityAdd BLAST39
Transmembranei40 – 60HelicalSequence analysisAdd BLAST21
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Topological domaini85 – 92CytoplasmicBy similarity8
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Topological domaini136 – 188CytoplasmicBy similarityAdd BLAST53

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi110Y → A: Significant decrease in interaction with ARL6IP1 and no influence on SLC1A1/EAAC1-mediated glutamate transport; when associated with A-112. 1 Publication1
Mutagenesisi112L → A: Significant decrease in interaction with ARL6IP1 and no influence on SLC1A1/EAAC1-mediated glutamate transport; when associated with A-110. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208841 – 188PRA1 family protein 3Add BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8R5J9
MaxQBiQ8R5J9
PaxDbiQ8R5J9
PeptideAtlasiQ8R5J9
PRIDEiQ8R5J9

PTM databases

iPTMnetiQ8R5J9
PhosphoSitePlusiQ8R5J9

Expressioni

Tissue specificityi

Expressed in the cerebral cortex, cerebellum, hippocampus, olfactory bulbs, medulla oblongate and limbic system (at protein level) (PubMed:18684713). Ubiquitous.3 Publications

Inductioni

By methyl-beta-cyclodextrin. Up-regulated upon chronic morphine injection, in amygdala only, other brain regions remain unaffected. Induction by morphine may affect glutamate uptake in the amygdala, causing mice to develop morphine tolerance and dependence (PubMed:12438930). Was originally reported to be induced by retinoic acid (PubMed:12562531).1 Publication1 Publication

Gene expression databases

BgeeiENSMUSG00000035199 Expressed in 290 organ(s), highest expression level in diaphragm
GenevisibleiQ8R5J9 MM

Interactioni

Subunit structurei

Homodimer. Heterodimer with ARL6IP1 (PubMed:18684713). Forms multimers. Interacts with prenylated RAB1A and RAB3A. Does not interact with VAMP1, VAMP2 or VAMP3 (By similarity). Interacts with ARL6 (PubMed:10508919). Interacts with SLC1A1/EAAC1 (PubMed:18684713, PubMed:12119102).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8R5J9, 3 interactors
MINTiQ8R5J9
STRINGi10090.ENSMUSP00000041503

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 117Required for homodimer formation and heterodimer formation with ARL6IP11 PublicationAdd BLAST15
Regioni136 – 188Targeting to endoplasmic reticulum membraneBy similarityAdd BLAST53

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4050 Eukaryota
ENOG4111MUC LUCA
GeneTreeiENSGT00390000008631
HOGENOMiHOG000231363
HOVERGENiHBG053661
InParanoidiQ8R5J9
KOiK20393
OMAiISNLMYY
OrthoDBiEOG091G0QS5
PhylomeDBiQ8R5J9
TreeFamiTF105479

Family and domain databases

InterProiView protein in InterPro
IPR004895 Prenylated_rab_accept_PRA1
PfamiView protein in Pfam
PF03208 PRA1, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R5J9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDVNLAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV
60 70 80 90 100
AAMMISVVGF LSPFNMILGG VIVVLVFMGF VWAAHNKDIL RRMKKQYPTA
110 120 130 140 150
FVMVVMLASY FLISMFGGVM VFVFGITLPL LLMFIHASLR LRNLKNKLEN
160 170 180
KMEGIGLKKT PMGIILDALE QQEDNINKFA DYISKARE
Length:188
Mass (Da):21,558
Last modified:January 4, 2005 - v2
Checksum:i5A679FC5071319F0
GO

Sequence cautioni

The sequence AAD33050 differs from that shown. Reason: Frameshift at positions 94 and 108.Curated
The sequence AAD33050 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11W → R in BAC40209 (PubMed:16141072).Curated1
Sequence conflicti14F → S in BAC40209 (PubMed:16141072).Curated1
Sequence conflicti19Missing (PubMed:10508919).Curated1
Sequence conflicti86Missing (PubMed:10508919).Curated1
Sequence conflicti103M → T in BAB25717 (PubMed:16141072).Curated1
Sequence conflicti117G → R (PubMed:10508919).Curated1
Sequence conflicti140R → K in BAB25717 (PubMed:16141072).Curated1
Sequence conflicti163G → V in AAL77876 (PubMed:12119102).Curated1
Sequence conflicti178K → E in BAC40209 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF421860 mRNA Translation: AAL77876.1
D87211 mRNA Translation: BAC24103.1
AF265214 mRNA Translation: AAL74056.1
AK005259 mRNA Translation: BAB23912.1
AK008519 mRNA Translation: BAB25717.1
AK088205 mRNA Translation: BAC40209.1
AK076519 mRNA Translation: BAC36376.1
BC003897 mRNA Translation: AAH03897.1
AF133912 mRNA Translation: AAD33050.1 Sequence problems.
CCDSiCCDS20384.1
RefSeqiNP_075368.1, NM_022992.2
UniGeneiMm.291014

Genome annotation databases

EnsembliENSMUST00000044681; ENSMUSP00000041503; ENSMUSG00000035199
GeneIDi65106
KEGGimmu:65106
UCSCiuc009dar.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF421860 mRNA Translation: AAL77876.1
D87211 mRNA Translation: BAC24103.1
AF265214 mRNA Translation: AAL74056.1
AK005259 mRNA Translation: BAB23912.1
AK008519 mRNA Translation: BAB25717.1
AK088205 mRNA Translation: BAC40209.1
AK076519 mRNA Translation: BAC36376.1
BC003897 mRNA Translation: AAH03897.1
AF133912 mRNA Translation: AAD33050.1 Sequence problems.
CCDSiCCDS20384.1
RefSeqiNP_075368.1, NM_022992.2
UniGeneiMm.291014

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8R5J9, 3 interactors
MINTiQ8R5J9
STRINGi10090.ENSMUSP00000041503

PTM databases

iPTMnetiQ8R5J9
PhosphoSitePlusiQ8R5J9

Proteomic databases

EPDiQ8R5J9
MaxQBiQ8R5J9
PaxDbiQ8R5J9
PeptideAtlasiQ8R5J9
PRIDEiQ8R5J9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044681; ENSMUSP00000041503; ENSMUSG00000035199
GeneIDi65106
KEGGimmu:65106
UCSCiuc009dar.1 mouse

Organism-specific databases

CTDi10550
MGIiMGI:1929501 Arl6ip5

Phylogenomic databases

eggNOGiKOG4050 Eukaryota
ENOG4111MUC LUCA
GeneTreeiENSGT00390000008631
HOGENOMiHOG000231363
HOVERGENiHBG053661
InParanoidiQ8R5J9
KOiK20393
OMAiISNLMYY
OrthoDBiEOG091G0QS5
PhylomeDBiQ8R5J9
TreeFamiTF105479

Enzyme and pathway databases

ReactomeiR-MMU-210500 Glutamate Neurotransmitter Release Cycle

Miscellaneous databases

ChiTaRSiArl6ip5 mouse
PROiPR:Q8R5J9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035199 Expressed in 290 organ(s), highest expression level in diaphragm
GenevisibleiQ8R5J9 MM

Family and domain databases

InterProiView protein in InterPro
IPR004895 Prenylated_rab_accept_PRA1
PfamiView protein in Pfam
PF03208 PRA1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRAF3_MOUSE
AccessioniPrimary (citable) accession number: Q8R5J9
Secondary accession number(s): Q8C2Q1
, Q9D838, Q9DB37, Q9WUG9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 4, 2005
Last modified: November 7, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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