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Protein

Wiskott-Aldrich syndrome protein family member 1

Gene

Wasf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 1
Short name:
WASP family protein member 1
Alternative name(s):
Protein WAVE-1
Gene namesi
Name:Wasf1
Synonyms:Wave1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1890563 Wasf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889931 – 559Wiskott-Aldrich syndrome protein family member 1Add BLAST559

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei341Omega-N-methylarginine; alternateCombined sources1
Modified residuei489PhosphoserineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ8R5H6
PaxDbiQ8R5H6
PeptideAtlasiQ8R5H6
PRIDEiQ8R5H6

PTM databases

iPTMnetiQ8R5H6
PhosphoSitePlusiQ8R5H6

Miscellaneous databases

PMAP-CutDBiQ8R5H6

Expressioni

Tissue specificityi

Highly expressed in brain.2 Publications

Gene expression databases

BgeeiENSMUSG00000019831 Expressed in 248 organ(s), highest expression level in dentate gyrus of hippocampal formation
CleanExiMM_WASF1
GenevisibleiQ8R5H6 MM

Interactioni

Subunit structurei

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites (By similarity).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219971, 18 interactors
CORUMiQ8R5H6
ELMiQ8R5H6
IntActiQ8R5H6, 15 interactors
MINTiQ8R5H6
STRINGi10090.ENSMUSP00000019975

Structurei

3D structure databases

ProteinModelPortaliQ8R5H6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini497 – 514WH2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi278 – 283Poly-Pro6
Compositional biasi322 – 332Poly-ProAdd BLAST11
Compositional biasi348 – 359Poly-ProAdd BLAST12
Compositional biasi369 – 374Poly-Pro6
Compositional biasi424 – 435Poly-ProAdd BLAST12

Domaini

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.By similarity

Sequence similaritiesi

Belongs to the SCAR/WAVE family.Curated

Phylogenomic databases

eggNOGiKOG1830 Eukaryota
ENOG410ZMD1 LUCA
GeneTreeiENSGT00730000110593
HOGENOMiHOG000021456
HOVERGENiHBG058482
InParanoidiQ8R5H6
KOiK05753
OMAiGPHAPLM
OrthoDBiEOG091G08O1
PhylomeDBiQ8R5H6
TreeFamiTF315031

Family and domain databases

InterProiView protein in InterPro
IPR028288 SCAR/WAVE_fam
IPR003124 WH2_dom
PANTHERiPTHR12902 PTHR12902, 1 hit
PfamiView protein in Pfam
PF02205 WH2, 1 hit
SMARTiView protein in SMART
SM00246 WH2, 1 hit
PROSITEiView protein in PROSITE
PS51082 WH2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8R5H6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED
60 70 80 90 100
IFGELFNEAH SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA
110 120 130 140 150
FRSSTIQDQQ LFDRKTLPIP LQETYDVCEQ PPPLNILTPY RDDGKEGLKF
160 170 180 190 200
YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK QKNLDRPHEP EKVPRAPHDR
210 220 230 240 250
RREWQKLAQG PELAEDDADL LHKHIEVANG PASHYETRPQ TYVDHMDGSY
260 270 280 290 300
SLSALPFSQM SELLTRAEER VLVRPHEPPP PPPMHGAGDA KPTPTCISSA
310 320 330 340 350
TGLIENRPQS PAAGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP
360 370 380 390 400
PVPPPPPPPA TALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV
410 420 430 440 450
ARAAPVCETV PVHPLPQGEV QGLPPPPPPP PLPPPGIRPS SPVAVAALAH
460 470 480 490 500
PPSGLHPAPS TAPGPHAPLM PPSPPSQVLP ASEPKRHPST LPVISDARSV
510 520 530 540 550
LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA VEYSDSEDDS

EFDEVDWLE
Length:559
Mass (Da):61,509
Last modified:February 1, 2005 - v2
Checksum:i8746910987D80D16
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2P → L in AAL75574 (PubMed:12062808).Curated1
Sequence conflicti324P → S in AAH16896 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290877 mRNA Translation: AAG02214.1
AF467773 mRNA Translation: AAL75574.1
BC016896 mRNA Translation: AAH16896.1
BC053423 mRNA Translation: AAH53423.1
CCDSiCCDS23801.1
RefSeqiNP_114083.1, NM_031877.3
XP_006512995.1, XM_006512932.2
UniGeneiMm.41353

Genome annotation databases

EnsembliENSMUST00000019975; ENSMUSP00000019975; ENSMUSG00000019831
ENSMUST00000105509; ENSMUSP00000101148; ENSMUSG00000019831
GeneIDi83767
KEGGimmu:83767
UCSCiuc007exi.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290877 mRNA Translation: AAG02214.1
AF467773 mRNA Translation: AAL75574.1
BC016896 mRNA Translation: AAH16896.1
BC053423 mRNA Translation: AAH53423.1
CCDSiCCDS23801.1
RefSeqiNP_114083.1, NM_031877.3
XP_006512995.1, XM_006512932.2
UniGeneiMm.41353

3D structure databases

ProteinModelPortaliQ8R5H6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219971, 18 interactors
CORUMiQ8R5H6
ELMiQ8R5H6
IntActiQ8R5H6, 15 interactors
MINTiQ8R5H6
STRINGi10090.ENSMUSP00000019975

PTM databases

iPTMnetiQ8R5H6
PhosphoSitePlusiQ8R5H6

Proteomic databases

MaxQBiQ8R5H6
PaxDbiQ8R5H6
PeptideAtlasiQ8R5H6
PRIDEiQ8R5H6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019975; ENSMUSP00000019975; ENSMUSG00000019831
ENSMUST00000105509; ENSMUSP00000101148; ENSMUSG00000019831
GeneIDi83767
KEGGimmu:83767
UCSCiuc007exi.2 mouse

Organism-specific databases

CTDi8936
MGIiMGI:1890563 Wasf1

Phylogenomic databases

eggNOGiKOG1830 Eukaryota
ENOG410ZMD1 LUCA
GeneTreeiENSGT00730000110593
HOGENOMiHOG000021456
HOVERGENiHBG058482
InParanoidiQ8R5H6
KOiK05753
OMAiGPHAPLM
OrthoDBiEOG091G08O1
PhylomeDBiQ8R5H6
TreeFamiTF315031

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Miscellaneous databases

ChiTaRSiWasf1 mouse
PMAP-CutDBiQ8R5H6
PROiPR:Q8R5H6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019831 Expressed in 248 organ(s), highest expression level in dentate gyrus of hippocampal formation
CleanExiMM_WASF1
GenevisibleiQ8R5H6 MM

Family and domain databases

InterProiView protein in InterPro
IPR028288 SCAR/WAVE_fam
IPR003124 WH2_dom
PANTHERiPTHR12902 PTHR12902, 1 hit
PfamiView protein in Pfam
PF02205 WH2, 1 hit
SMARTiView protein in SMART
SM00246 WH2, 1 hit
PROSITEiView protein in PROSITE
PS51082 WH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWASF1_MOUSE
AccessioniPrimary (citable) accession number: Q8R5H6
Secondary accession number(s): Q91W51, Q9ERQ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: November 7, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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