UniProtKB - Q8R500 (MFN2_RAT)
Mitofusin-2
Mfn2
Functioni
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 305 | GTPBy similarity | 1 | |
Binding sitei | 307 | GTPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 106 – 111 | GTPBy similarity | 6 | |
Nucleotide bindingi | 258 – 261 | GTPBy similarity | 4 |
GO - Molecular functioni
- GTPase activity Source: GO_Central
- GTPase binding Source: RGD
- GTP binding Source: RGD
- identical protein binding Source: RGD
- proteoglycan binding Source: RGD
- small GTPase binding Source: RGD
- ubiquitin protein ligase binding Source: RGD
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- blastocyst formation Source: RGD
- camera-type eye morphogenesis Source: RGD
- cellular response to dexamethasone stimulus Source: RGD
- cellular response to glucose stimulus Source: RGD
- cellular response to hypoxia Source: RGD
- cellular response to ionizing radiation Source: RGD
- cellular response to L-glutamate Source: RGD
- cellular response to low-density lipoprotein particle stimulus Source: RGD
- cellular response to manganese ion Source: RGD
- cellular response to mercaptoethanol Source: RGD
- cellular response to oxygen-glucose deprivation Source: RGD
- cellular response to testosterone stimulus Source: RGD
- intracellular distribution of mitochondria Source: RGD
- male gonad development Source: RGD
- mitochondrial calcium ion homeostasis Source: RGD
- mitochondrial fusion Source: RGD
- mitochondrial membrane organization Source: UniProtKB
- mitochondrion localization Source: RGD
- mitochondrion morphogenesis Source: RGD
- mitochondrion organization Source: RGD
- mitotic cell cycle Source: RGD
- mitotic cell cycle arrest Source: RGD
- negative regulation of cardiac muscle cell apoptotic process Source: RGD
- negative regulation of cell cycle Source: RGD
- negative regulation of cell death Source: RGD
- negative regulation of cell population proliferation Source: RGD
- negative regulation of cell size Source: RGD
- negative regulation of glial cell proliferation Source: RGD
- negative regulation of mitochondrial fission Source: RGD
- negative regulation of neuron death Source: RGD
- negative regulation of Ras protein signal transduction Source: RGD
- negative regulation of reactive oxygen species biosynthetic process Source: RGD
- negative regulation of release of cytochrome c from mitochondria Source: RGD
- negative regulation of sarcomere organization Source: RGD
- negative regulation of smooth muscle cell proliferation Source: UniProtKB
- negative regulation of vascular associated smooth muscle cell proliferation Source: RGD
- parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization Source: RGD
- positive regulation of cellular respiration Source: RGD
- positive regulation of chromatin binding Source: RGD
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of dendritic spine morphogenesis Source: RGD
- positive regulation of estradiol secretion Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of glucose import Source: RGD
- positive regulation of hydrogen peroxide biosynthetic process Source: RGD
- positive regulation of insulin receptor signaling pathway Source: RGD
- positive regulation of mitochondrial membrane potential Source: RGD
- positive regulation of ovarian follicle development Source: RGD
- positive regulation of progesterone secretion Source: RGD
- positive regulation of reactive oxygen species biosynthetic process Source: RGD
- positive regulation of vascular associated smooth muscle cell apoptotic process Source: RGD
- positive regulation of vascular associated smooth muscle cell proliferation Source: RGD
- protein localization to phagophore assembly site Source: RGD
- protein targeting to mitochondrion Source: UniProtKB
- response to axon injury Source: RGD
- response to cadmium ion Source: RGD
- response to calcium ion Source: RGD
- response to D-galactose Source: RGD
- response to electrical stimulus Source: RGD
- response to fatty acid Source: RGD
- response to flavonoid Source: RGD
- response to hypobaric hypoxia Source: RGD
- response to L-arginine Source: RGD
- response to muscle activity Source: RGD
- response to nutrient levels Source: RGD
- response to testosterone Source: RGD
- response to unfolded protein Source: UniProtKB-KW
- spermatogenesis Source: RGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Apoptosis, Autophagy, Unfolded protein response |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-5205685, PINK1-PRKN Mediated Mitophagy R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: Mitofusin-2 (EC:3.6.5.-)Alternative name(s): Mitochondrial transmembrane GTPase FZO1A Short name: Protein HSG Transmembrane GTPase MFN2 |
Gene namesi | Name:Mfn2 Synonyms:Fzo1a |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628843, Mfn2 |
Subcellular locationi
Mitochondrion
- Mitochondrion outer membrane 2 Publications; Multi-pass membrane protein 1 Publication
Note: Colocalizes with BAX during apoptosis.By similarity
Cytoskeleton
- microtubule cytoskeleton Source: RGD
Cytosol
- cytosol Source: UniProtKB
Mitochondrion
- intrinsic component of mitochondrial outer membrane Source: UniProtKB
- mitochondrial outer membrane Source: RGD
- mitochondrion Source: RGD
Other locations
- cytoplasm Source: RGD
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 604 | CytoplasmicSequence analysisAdd BLAST | 604 | |
Transmembranei | 605 – 625 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 626 | Mitochondrial intermembraneSequence analysis | 1 | |
Transmembranei | 627 – 647 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 648 – 757 | CytoplasmicSequence analysisAdd BLAST | 110 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion outer membranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 109 | K → T: Induces mitochondria fragmentation when overexpressed. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127677 | 1 – 757 | Mitofusin-2Add BLAST | 757 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 111 | Phosphothreonine; by PINK1By similarity | 1 | |
Modified residuei | 442 | Phosphoserine; by PINK1By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q8R500 |
PaxDbi | Q8R500 |
PRIDEi | Q8R500 |
PTM databases
iPTMneti | Q8R500 |
PhosphoSitePlusi | Q8R500 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Forms homomultimers and heteromultimers with MFN1 (PubMed:14561718). Oligomerization is essential for mitochondrion fusion (Probable).
Interacts with VAT1 (PubMed:17105775).
Interacts with STOML2; may form heterooligomers (By similarity).
Interacts (phosphorylated) with PRKN (By similarity).
Interacts with EIF2AK3 (By similarity).
Interacts with THG1L; THG1L probably functions as a guanyl-nucleotide exchange factor/GEF, activating MFN2.
By similarityCurated2 PublicationsGO - Molecular functioni
- GTPase binding Source: RGD
- identical protein binding Source: RGD
- proteoglycan binding Source: RGD
- small GTPase binding Source: RGD
- ubiquitin protein ligase binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000059463 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 93 – 342 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 250 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 30 – 94 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 65 | |
Regioni | 103 – 110 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 129 – 130 | G2 motifPROSITE-ProRule annotation | 2 | |
Regioni | 199 – 202 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 258 – 261 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 288 | G5 motifPROSITE-ProRule annotation | 1 | |
Regioni | 359 – 385 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 27 | |
Regioni | 722 – 753 | Part of a helix bundle domain, formed by helices from N-terminal and C-terminal regionsBy similarityAdd BLAST | 32 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 406 – 435 | Sequence analysisAdd BLAST | 30 | |
Coiled coili | 696 – 738 | Sequence analysisAdd BLAST | 43 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0448, Eukaryota |
InParanoidi | Q8R500 |
PhylomeDBi | Q8R500 |
Family and domain databases
InterProi | View protein in InterPro IPR022812, Dynamin_SF IPR006884, Fzo/mitofusin_HR2 IPR030381, G_DYNAMIN_dom IPR027089, Mitofusin-2 IPR027094, Mitofusin_fam IPR027417, P-loop_NTPase |
PANTHERi | PTHR10465, PTHR10465, 1 hit PTHR10465:SF1, PTHR10465:SF1, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit PF04799, Fzo_mitofusin, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSLLFSRCNS IVTVKKDKRH MAEVNASPLK HFVTAKKKIN GIFEQLGAYI
60 70 80 90 100
QESAGFLEDT HRNTELDPVT TEEQVLDVKG YLSKVRGISE VLARRHMKVA
110 120 130 140 150
FFGRTSNGKS TVINAMLWDK VLPSGIGHTT NCFLRVGGTD GHEAFLLTEG
160 170 180 190 200
SEEKKSVKTV NQLAHALHQD EQLHAGSLVS VMWPNSKCPL LKDGLVLMDS
210 220 230 240 250
PGIDVTTELD SWIDKFCLDA DVFVLVANSE STLMQTEKQF FHKVSERLSR
260 270 280 290 300
PNIFILNNRW DASASEPEYM EEVRRQHMER CTSFLVDELG VVDRAQAGDR
310 320 330 340 350
IFFVSAKEVL SARVQKAQGM PEGGGALAEG FQVRMFEFQN FERRFEECIS
360 370 380 390 400
QSAVKTKFEQ HTVRAKQIAE AVRLIMDSLH IAAQEQRVYC LEMREERQDR
410 420 430 440 450
LRFIDKQLEL LAQDYKLRIK QMTEEVERQV STAMAEEIRR LSVLVDEYQM
460 470 480 490 500
DFHPSPVVLK VYKNELHRHI EEGLGRNMSD RCSTAIASSL QTMQQDMIDG
510 520 530 540 550
LKPLLPVSVR NQIDMLVPRQ CFSLSYDLNC DKLCADFQED IEFHFSLGWT
560 570 580 590 600
MLVNRFLGPK NSRRALLGYN DQVQRPLPLT PANPSMPPLP QGSLTQEELM
610 620 630 640 650
VSMVTGLASL TSRTSMGILV VGGVVWKAVG WRLIALSFGL YGLLYVYERL
660 670 680 690 700
TWTTRAKERA FKRQFVEYAS EKLQLIISYT GSNCSHQVQQ ELSGTFAHLC
710 720 730 740 750
QQVDITRDNL EQEIAAMNKK VEALDSLQSK AKLLRNKAGW LDSELNMFIH
QYLQPSR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ6IRL2 | Q6IRL2_RAT | Mitofusin 2, isoform CRA_a | LOC100911485 Mfn2, rCG_31319 | 757 | Annotation score: | ||
F7FHF7 | F7FHF7_RAT | Mitofusin-2 | LOC100911485 Mfn2 | 762 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 183 | W → L in AAB87720 (PubMed:15322553).Curated | 1 | |
Sequence conflicti | 194 | G → D in AAB87720 (PubMed:15322553).Curated | 1 | |
Sequence conflicti | 265 | S → P in AAB87720 (PubMed:15322553).Curated | 1 | |
Sequence conflicti | 404 | I → M in AAB87720 (PubMed:15322553).Curated | 1 | |
Sequence conflicti | 431 | S → T in AAB87720 (PubMed:15322553).Curated | 1 | |
Sequence conflicti | 756 | S → G in AAB87720 (PubMed:15322553).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB084165 mRNA Translation: BAB90982.1 U41803 mRNA Translation: AAB87720.3 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB084165 mRNA Translation: BAB90982.1 U41803 mRNA Translation: AAB87720.3 |
3D structure databases
SMRi | Q8R500 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000059463 |
PTM databases
iPTMneti | Q8R500 |
PhosphoSitePlusi | Q8R500 |
Proteomic databases
jPOSTi | Q8R500 |
PaxDbi | Q8R500 |
PRIDEi | Q8R500 |
Protocols and materials databases
ABCDi | Q8R500, 1 sequenced antibody |
Organism-specific databases
RGDi | 628843, Mfn2 |
Phylogenomic databases
eggNOGi | KOG0448, Eukaryota |
InParanoidi | Q8R500 |
PhylomeDBi | Q8R500 |
Enzyme and pathway databases
Reactomei | R-RNO-5205685, PINK1-PRKN Mediated Mitophagy R-RNO-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
PROi | PR:Q8R500 |
Family and domain databases
InterProi | View protein in InterPro IPR022812, Dynamin_SF IPR006884, Fzo/mitofusin_HR2 IPR030381, G_DYNAMIN_dom IPR027089, Mitofusin-2 IPR027094, Mitofusin_fam IPR027417, P-loop_NTPase |
PANTHERi | PTHR10465, PTHR10465, 1 hit PTHR10465:SF1, PTHR10465:SF1, 1 hit |
Pfami | View protein in Pfam PF00350, Dynamin_N, 1 hit PF04799, Fzo_mitofusin, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51718, G_DYNAMIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MFN2_RAT | |
Accessioni | Q8R500Primary (citable) accession number: Q8R500 Secondary accession number(s): O09013 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2004 |
Last sequence update: | June 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families